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The map label for this gene is eda [H]

Identifier: 37678246

GI number: 37678246

Start: 73238

End: 73849

Strand: Direct

Name: eda [H]

Synonym: VV0062

Alternate gene names: 37678246

Gene position: 73238-73849 (Clockwise)

Preceding gene: 37678245

Following gene: 37678247

Centisome position: 2.18

GC content: 52.78

Gene sequence:

>612_bases
ATGACAACACTTGAACAACGATTAAGAGAGATCAAAATCGTCCCAGTTATCGCTATCAACGATGTAGCACACGCCTTGCC
ATTGGCAAAAGTGTTGGTAGAAAACGGTTTACCCTGTGCGGAAGTGACATTCCGCACGGAAGCGGCGGCGGAATCCATTC
GCATCATGCGCGAAGCCTACCCTGATTTGTTGATTGGTGCGGGCACGGTATTAACCACAGAACAAGTGGACATCGCCATC
GAAGCGGGCGTGGATTTCATTGTCAGCCCAGGCTTTAACCCAACCACCGTGAAATACTGTCAGCAACGCGGCGTGACCAT
TGTACCGGGCGTCAACAACCCAAGCTTGGTTGAGCAAGCGATGGAAATGGGTCTACGTACTCTGAAGTTCTTCCCTGCTG
AGCCATCAGGCGGCGTCAACATGCTTAAAGCGCTGACGGCGGTTTACCCAGTGGAATTCATGCCAACAGGTGGCGTGAGC
CCATCTAACGTGGAAGATTATCTCGCGCTGAAATCGGTTGTCGCGTGTGGCGGTACTTGGATGGTTCCAACCTCCATGAT
GGACAGCGGCGATTGGGAAGGCATTGCGGAACTGGTTCGCGCGGTGAAGTAA

Upstream 100 bases:

>100_bases
GAAAACCACGCTGAAAACTTGGACGGTAATGGAAACCTTGCTTGGTGGTATTGCTTTCCTTATCGTCGCTGTATTGAGCT
TTATTCTTTAGAGGTTATCT

Downstream 100 bases:

>100_bases
ACACGATTTTTGCACAGCAATAAGGCTATAAATTAAATTGATAGCCTTATTGTTTGATTTTTAAGTAGTTATCCGTTCAA
GCTTTATATTTTTTATTGTT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTTLEQRLREIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTEAAAESIRIMREAYPDLLIGAGTVLTTEQVDIAI
EAGVDFIVSPGFNPTTVKYCQQRGVTIVPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGVNMLKALTAVYPVEFMPTGGVS
PSNVEDYLALKSVVACGGTWMVPTSMMDSGDWEGIAELVRAVK

Sequences:

>Translated_203_residues
MTTLEQRLREIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTEAAAESIRIMREAYPDLLIGAGTVLTTEQVDIAI
EAGVDFIVSPGFNPTTVKYCQQRGVTIVPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGVNMLKALTAVYPVEFMPTGGVS
PSNVEDYLALKSVVACGGTWMVPTSMMDSGDWEGIAELVRAVK
>Mature_202_residues
TTLEQRLREIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTEAAAESIRIMREAYPDLLIGAGTVLTTEQVDIAIE
AGVDFIVSPGFNPTTVKYCQQRGVTIVPGVNNPSLVEQAMEMGLRTLKFFPAEPSGGVNMLKALTAVYPVEFMPTGGVSP
SNVEDYLALKSVVACGGTWMVPTSMMDSGDWEGIAELVRAVK

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=178, Percent_Identity=43.2584269662921, Blast_Score=159, Evalue=9e-41,
Organism=Escherichia coli, GI48994954, Length=148, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21766; Mature: 21634

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLEQRLREIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTEAAAESIRIMREAY
CCCHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHC
PDLLIGAGTVLTTEQVDIAIEAGVDFIVSPGFNPTTVKYCQQRGVTIVPGVNNPSLVEQA
CCCEECCCCEEEECHHEEEEECCCCEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHHHH
MEMGLRTLKFFPAEPSGGVNMLKALTAVYPVEFMPTGGVSPSNVEDYLALKSVVACGGTW
HHHHHHHHEECCCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHCCCCE
MVPTSMMDSGDWEGIAELVRAVK
ECCHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTLEQRLREIKIVPVIAINDVAHALPLAKVLVENGLPCAEVTFRTEAAAESIRIMREAY
CCHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHC
PDLLIGAGTVLTTEQVDIAIEAGVDFIVSPGFNPTTVKYCQQRGVTIVPGVNNPSLVEQA
CCCEECCCCEEEECHHEEEEECCCCEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHHHH
MEMGLRTLKFFPAEPSGGVNMLKALTAVYPVEFMPTGGVSPSNVEDYLALKSVVACGGTW
HHHHHHHHEECCCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHCCCCE
MVPTSMMDSGDWEGIAELVRAVK
ECCHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]