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The map label for this gene is dppA [H]

Identifier: 37678228

GI number: 37678228

Start: 52677

End: 54233

Strand: Direct

Name: dppA [H]

Synonym: VV0044

Alternate gene names: 37678228

Gene position: 52677-54233 (Clockwise)

Preceding gene: 37678227

Following gene: 37678229

Centisome position: 1.57

GC content: 49.52

Gene sequence:

>1557_bases
ATGAAAACCATGAAAAGCAAACTGACCGTGGCTTTAATGGCTGCAGGCCTAAGCCTGAGTGCTGCTGCGGCAGACATCAA
AGTTGCTTATGATGCAGATCCAGTGTCACTTGACCCGCATGAGCAGTTGTCTGGTGGTACGCTACAAATGTCGCACATGG
TGTTTGATCCACTAGTTCGTTATACCCAGAAATTGGATTTTGAACCTCGCTTGGCTGAAAAATGGGAACGTGTCAATGAC
ACCACTTACCGCTTCCAGCTACGTAAAGGGGTGAAGTTCCATTCCGGTAACGAACTGACGGCAGATGATGTCGTGTGGAC
ATTCGATCGTCTGAAAGATTCTCCAGACTTTAAAGCGATCTTTGAACCGTATGAAAAAATGGTCAAAGTGGACGATTACA
CCGTTGAGTTGGTGTCCAAAGGGGCTTACCCATTGGTGCTACAAACCGCCACTTACATCTTCCCGATGGACAGCAAGTTC
TACTCTGGCACGACAGCCGATGGCAAAGACAAAGCGGAAGTGGTGAAGCACGGTAACTCATTTGCTTCGACCAACGTTTC
TGGTACGGGTCCGTTCATCGTCACTTCTCGTGAGCAAGGCGTGAAAGTAGAGTTTGAGCGCTTTAAAGATTACTGGGACA
AAGAGTCAAAAGGTAACGTTGATAAGCTGACGCTTGTGCCAATCAAAGAAGACGCGACTCGTGTCGCTGCGCTCCTTTCT
GGTGGTGTTGACATGATTGCGCCAGTGGCACCTAACGATCACCAACGCGTGAAAGATGCGAAGGGCATCGATCTTGTGAC
GCTGCCTGGTACGCGTATCATCACTTTCCAAATGAACCAAAACAGCAACGAAGCGCTGAAAGATGTGCGCGTGCGTCAGG
CGATCGTTCATGCCATCAACAATCAAGGTATCGTTGATAAGATCATGAAAGGTTTCGCGACAGCGGCTGGCCAGCAGGGC
CCTGAAGGCTACGCGGGCTACAACGCGGAGCTGGTTCCTCGTTTCGATCTGAAAAAAGCGAAGCAGTTGATGAAAGAAGC
GGGTTATGAGAAAGGCTTTACGCTGACGATGATCGCGCCGAACAACCGTTACGTTAACGATGCGAAAGTGGCTCAAGCGG
CGTCTGCCATGCTATCGAAAATCGGTATCAAGGTAGACCTAAAGACCATGCCTAAAGCGCAATACTGGCCTGAGTTTGAT
AAGTGTGCAGCAGACATGTTGATGATCGGCTGGCACTCAGACACAGAAGATTCGGCGAACTTCTCTGAGTTCCTCACCAT
GACGCGTAACGAAGAAACGGGCCGTGGTCAGTACAACTGTGGTCACTACTCAAACCCAGAAGTGGACAAGTTGGTGGAAG
CGGCGAACGTAGAAACCGATCCTGCTAAACGTGCAGCGATGCTACAGAAAGTCGAAACCACGCTGTACAACGAAGCAGCA
TTCGTTCCTCTACACTGGCAAAACCTAGCGTGGGGCGCGAAATCTACGCTGGATATCAAACCAATTGTCAACGCGATGGA
CTTCCCATACTTTGGTGACTTGGTAGTTAAAGAGTAA

Upstream 100 bases:

>100_bases
GAGGGAAGAGGTGAAAACGGCATAACCAAGCTTGCTTGGCACAATAACAGACGCAAATACAACAACTAGGGATTCGGATT
CCCGCATAAGGAGTTATGCA

Downstream 100 bases:

>100_bases
CACTCGCTTTCTCCCACTCAAGCGTTGGGTGGGATGAGTGATTTTAGGCGCTCAACTTTGTTTCAGTCGGGTTGAGCGCC
GTTTTCAGACTGAAGTTTAT

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKTMKSKLTVALMAAGLSLSAAAADIKVAYDADPVSLDPHEQLSGGTLQMSHMVFDPLVRYTQKLDFEPRLAEKWERVND
TTYRFQLRKGVKFHSGNELTADDVVWTFDRLKDSPDFKAIFEPYEKMVKVDDYTVELVSKGAYPLVLQTATYIFPMDSKF
YSGTTADGKDKAEVVKHGNSFASTNVSGTGPFIVTSREQGVKVEFERFKDYWDKESKGNVDKLTLVPIKEDATRVAALLS
GGVDMIAPVAPNDHQRVKDAKGIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAINNQGIVDKIMKGFATAAGQQG
PEGYAGYNAELVPRFDLKKAKQLMKEAGYEKGFTLTMIAPNNRYVNDAKVAQAASAMLSKIGIKVDLKTMPKAQYWPEFD
KCAADMLMIGWHSDTEDSANFSEFLTMTRNEETGRGQYNCGHYSNPEVDKLVEAANVETDPAKRAAMLQKVETTLYNEAA
FVPLHWQNLAWGAKSTLDIKPIVNAMDFPYFGDLVVKE

Sequences:

>Translated_518_residues
MKTMKSKLTVALMAAGLSLSAAAADIKVAYDADPVSLDPHEQLSGGTLQMSHMVFDPLVRYTQKLDFEPRLAEKWERVND
TTYRFQLRKGVKFHSGNELTADDVVWTFDRLKDSPDFKAIFEPYEKMVKVDDYTVELVSKGAYPLVLQTATYIFPMDSKF
YSGTTADGKDKAEVVKHGNSFASTNVSGTGPFIVTSREQGVKVEFERFKDYWDKESKGNVDKLTLVPIKEDATRVAALLS
GGVDMIAPVAPNDHQRVKDAKGIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAINNQGIVDKIMKGFATAAGQQG
PEGYAGYNAELVPRFDLKKAKQLMKEAGYEKGFTLTMIAPNNRYVNDAKVAQAASAMLSKIGIKVDLKTMPKAQYWPEFD
KCAADMLMIGWHSDTEDSANFSEFLTMTRNEETGRGQYNCGHYSNPEVDKLVEAANVETDPAKRAAMLQKVETTLYNEAA
FVPLHWQNLAWGAKSTLDIKPIVNAMDFPYFGDLVVKE
>Mature_518_residues
MKTMKSKLTVALMAAGLSLSAAAADIKVAYDADPVSLDPHEQLSGGTLQMSHMVFDPLVRYTQKLDFEPRLAEKWERVND
TTYRFQLRKGVKFHSGNELTADDVVWTFDRLKDSPDFKAIFEPYEKMVKVDDYTVELVSKGAYPLVLQTATYIFPMDSKF
YSGTTADGKDKAEVVKHGNSFASTNVSGTGPFIVTSREQGVKVEFERFKDYWDKESKGNVDKLTLVPIKEDATRVAALLS
GGVDMIAPVAPNDHQRVKDAKGIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAINNQGIVDKIMKGFATAAGQQG
PEGYAGYNAELVPRFDLKKAKQLMKEAGYEKGFTLTMIAPNNRYVNDAKVAQAASAMLSKIGIKVDLKTMPKAQYWPEFD
KCAADMLMIGWHSDTEDSANFSEFLTMTRNEETGRGQYNCGHYSNPEVDKLVEAANVETDPAKRAAMLQKVETTLYNEAA
FVPLHWQNLAWGAKSTLDIKPIVNAMDFPYFGDLVVKE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=505, Percent_Identity=29.3069306930693, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=25, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787762, Length=505, Percent_Identity=25.3465346534653, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081878, Length=549, Percent_Identity=24.0437158469945, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=24.7169811320755, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787551, Length=449, Percent_Identity=25.3897550111359, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787495, Length=523, Percent_Identity=25.2390057361377, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789887, Length=529, Percent_Identity=24.3856332703214, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57630; Mature: 57630

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMKSKLTVALMAAGLSLSAAAADIKVAYDADPVSLDPHEQLSGGTLQMSHMVFDPLVR
CCCHHHHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCHHHCCCCEEEHHHHHHHHHHH
YTQKLDFEPRLAEKWERVNDTTYRFQLRKGVKFHSGNELTADDVVWTFDRLKDSPDFKAI
HHHHCCCCCHHHHHHHHCCCCEEEEEEECCCEECCCCCCCHHHHHEEHHHHCCCCCHHHH
FEPYEKMVKVDDYTVELVSKGAYPLVLQTATYIFPMDSKFYSGTTADGKDKAEVVKHGNS
HHHHHHHEECCCEEEEEHHCCCCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCC
FASTNVSGTGPFIVTSREQGVKVEFERFKDYWDKESKGNVDKLTLVPIKEDATRVAALLS
CCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHH
GGVDMIAPVAPNDHQRVKDAKGIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAIN
CCCCEEECCCCCCHHHHHCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHC
NQGIVDKIMKGFATAAGQQGPEGYAGYNAELVPRFDLKKAKQLMKEAGYEKGFTLTMIAP
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEEECC
NNRYVNDAKVAQAASAMLSKIGIKVDLKTMPKAQYWPEFDKCAADMLMIGWHSDTEDSAN
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCEEEEECCCCCCCCCC
FSEFLTMTRNEETGRGQYNCGHYSNPEVDKLVEAANVETDPAKRAAMLQKVETTLYNEAA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEE
FVPLHWQNLAWGAKSTLDIKPIVNAMDFPYFGDLVVKE
EEEEECCCCCCCCCCCEEHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure
MKTMKSKLTVALMAAGLSLSAAAADIKVAYDADPVSLDPHEQLSGGTLQMSHMVFDPLVR
CCCHHHHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCHHHCCCCEEEHHHHHHHHHHH
YTQKLDFEPRLAEKWERVNDTTYRFQLRKGVKFHSGNELTADDVVWTFDRLKDSPDFKAI
HHHHCCCCCHHHHHHHHCCCCEEEEEEECCCEECCCCCCCHHHHHEEHHHHCCCCCHHHH
FEPYEKMVKVDDYTVELVSKGAYPLVLQTATYIFPMDSKFYSGTTADGKDKAEVVKHGNS
HHHHHHHEECCCEEEEEHHCCCCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCC
FASTNVSGTGPFIVTSREQGVKVEFERFKDYWDKESKGNVDKLTLVPIKEDATRVAALLS
CCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHH
GGVDMIAPVAPNDHQRVKDAKGIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAIN
CCCCEEECCCCCCHHHHHCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHC
NQGIVDKIMKGFATAAGQQGPEGYAGYNAELVPRFDLKKAKQLMKEAGYEKGFTLTMIAP
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEEECC
NNRYVNDAKVAQAASAMLSKIGIKVDLKTMPKAQYWPEFDKCAADMLMIGWHSDTEDSAN
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCEEEEECCCCCCCCCC
FSEFLTMTRNEETGRGQYNCGHYSNPEVDKLVEAANVETDPAKRAAMLQKVETTLYNEAA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEE
FVPLHWQNLAWGAKSTLDIKPIVNAMDFPYFGDLVVKE
EEEEECCCCCCCCCCCEEHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]