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The map label for this gene is prfA

Identifier: 37523990

GI number: 37523990

Start: 4646998

End: 4648092

Strand: Direct

Name: prfA

Synonym: glr4421

Alternate gene names: 37523990

Gene position: 4646998-4648092 (Clockwise)

Preceding gene: 37523989

Following gene: 37523991

Centisome position: 99.74

GC content: 60.91

Gene sequence:

>1095_bases
ATGGCCAGTCCCGATTTAATGGTTCAAAAGCTCAACGACATCGAGCGAACCTATAACGATTTGACTACTCGCCTAGGCGA
CCCAAGCCTGGCAAGCAACCCCCAGGAGATGCTGCGCATCGTGCGCATGCGCGCGGGCCTGGAGCGTACGGTCGAAGCCT
ACGACCATTGGCAGCGGCTGTGTGCAGAACGCAACGCCGTTCGCCAGATGATTCGCGACGAGCAAGATCCCGAGTTGCGC
GAACTGGCCGAAGAGGAGCACACCGGCCTCGAGGAGCGCATCGCCCGGCTCGAAGACGAGATTACGCTGCTGCTGTTGCC
GCGCGATCCCAATGACGACAAGAACGTCATGCTCGAAATTCGGGCTGGGACAGGCGGTGATGAGGCGGCTATCTTCGCGG
GCGACCTGATGCGCATGTACATCCGCTACGCGGAGGGCCAGGGCTGGTCGGTGAAGCTTGCCAGCGAATCGCCCGCCGAG
ATGGGCGGCTTCAAAGAAGTGATCCTCGAAATTCGCGGCGAGAGCGTGTATTCCAAGCTCAAATTCGAAGCGGGGGTCCA
CCGTGTCCAGCGCGTGCCGGTCACCGAGACCCAGGGGCGCGTGCATACTTCCACGGCCACGGTCGCGATCATGCCCGAGG
TCGAAGAAGTGGACGTCGAGATCAATCCCAACGACATCGAGATCACCACGACCCGTTCGGGCGGTGCGGGCGGTCAGAAC
GTCAACAAGGTAGAAACGGCGGTGCATCTGGTCCACAAGCCCTCCGGCATCCATATCCACTGCCAGGAGGAGCGCTCGCA
GTTGCAAAATAAAGCGCGCGCCATGCAGATTCTGCGCGTCAAGCTCTACGACATCAAACAGCGCGAGCAGCACGAGCAGG
TCTCCGGGCTGCGTCGTTCCCAGGTCGGTACCGGCGATCGATCTGAAAAAATCCGCACCTACAACTACAAAGACAACCGG
GTGACCGACCACCGCCTGAGCCAGAACTTCACCCTCAACACCGCCATTGAGGGCGAAATCGAAACGCTCATCCAATCGGC
CATCGCTGCCGCCCAGCGCGAACAACTCGCGGAACTGGCCCGCAGCAGCGGATGA

Upstream 100 bases:

>100_bases
CTAAAGCCGCCATGTACCGTTGTGCTCCTCTGCCGTTCCATTAGCGTCGCTGCCTGTTGACCGATCGACAGCACACTTTG
ATTTGCGGAGGAGCACACCC

Downstream 100 bases:

>100_bases
TTATCCCCGAAGCGCCGCCGGGCTTTCGCTCGGGTTTTGTCGCCATCGTCGGCCGCCCCAACGTCGGCAAATCGACGCTG
GTCAACCGGCTGGTGGGCGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MASPDLMVQKLNDIERTYNDLTTRLGDPSLASNPQEMLRIVRMRAGLERTVEAYDHWQRLCAERNAVRQMIRDEQDPELR
ELAEEEHTGLEERIARLEDEITLLLLPRDPNDDKNVMLEIRAGTGGDEAAIFAGDLMRMYIRYAEGQGWSVKLASESPAE
MGGFKEVILEIRGESVYSKLKFEAGVHRVQRVPVTETQGRVHTSTATVAIMPEVEEVDVEINPNDIEITTTRSGGAGGQN
VNKVETAVHLVHKPSGIHIHCQEERSQLQNKARAMQILRVKLYDIKQREQHEQVSGLRRSQVGTGDRSEKIRTYNYKDNR
VTDHRLSQNFTLNTAIEGEIETLIQSAIAAAQREQLAELARSSG

Sequences:

>Translated_364_residues
MASPDLMVQKLNDIERTYNDLTTRLGDPSLASNPQEMLRIVRMRAGLERTVEAYDHWQRLCAERNAVRQMIRDEQDPELR
ELAEEEHTGLEERIARLEDEITLLLLPRDPNDDKNVMLEIRAGTGGDEAAIFAGDLMRMYIRYAEGQGWSVKLASESPAE
MGGFKEVILEIRGESVYSKLKFEAGVHRVQRVPVTETQGRVHTSTATVAIMPEVEEVDVEINPNDIEITTTRSGGAGGQN
VNKVETAVHLVHKPSGIHIHCQEERSQLQNKARAMQILRVKLYDIKQREQHEQVSGLRRSQVGTGDRSEKIRTYNYKDNR
VTDHRLSQNFTLNTAIEGEIETLIQSAIAAAQREQLAELARSSG
>Mature_363_residues
ASPDLMVQKLNDIERTYNDLTTRLGDPSLASNPQEMLRIVRMRAGLERTVEAYDHWQRLCAERNAVRQMIRDEQDPELRE
LAEEEHTGLEERIARLEDEITLLLLPRDPNDDKNVMLEIRAGTGGDEAAIFAGDLMRMYIRYAEGQGWSVKLASESPAEM
GGFKEVILEIRGESVYSKLKFEAGVHRVQRVPVTETQGRVHTSTATVAIMPEVEEVDVEINPNDIEITTTRSGGAGGQNV
NKVETAVHLVHKPSGIHIHCQEERSQLQNKARAMQILRVKLYDIKQREQHEQVSGLRRSQVGTGDRSEKIRTYNYKDNRV
TDHRLSQNFTLNTAIEGEIETLIQSAIAAAQREQLAELARSSG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=44.8979591836735, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI34577120, Length=340, Percent_Identity=36.4705882352941, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI166795305, Length=187, Percent_Identity=45.4545454545455, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=42.9775280898876, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=32.6409495548961, Blast_Score=187, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=272, Percent_Identity=38.6029411764706, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=297, Percent_Identity=41.0774410774411, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=36.6863905325444, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_GLOVI (Q7ND15)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927367.1
- ProteinModelPortal:   Q7ND15
- SMR:   Q7ND15
- GeneID:   2602824
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr4421
- NMPDR:   fig|251221.1.peg.4421
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   GVIO251221:GLR4421-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41132; Mature: 41001

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPDLMVQKLNDIERTYNDLTTRLGDPSLASNPQEMLRIVRMRAGLERTVEAYDHWQRL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAERNAVRQMIRDEQDPELRELAEEEHTGLEERIARLEDEITLLLLPRDPNDDKNVMLEI
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEE
RAGTGGDEAAIFAGDLMRMYIRYAEGQGWSVKLASESPAEMGGFKEVILEIRGESVYSKL
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCHHHHHHHHCCHHHHHHH
KFEAGVHRVQRVPVTETQGRVHTSTATVAIMPEVEEVDVEINPNDIEITTTRSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCHHHEEEEECCCEEEEEEECCCCCCCCC
VNKVETAVHLVHKPSGIHIHCQEERSQLQNKARAMQILRVKLYDIKQREQHEQVSGLRRS
CHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGDRSEKIRTYNYKDNRVTDHRLSQNFTLNTAIEGEIETLIQSAIAAAQREQLAELA
CCCCCCCCCCEEEECCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
RSSG
HCCC
>Mature Secondary Structure 
ASPDLMVQKLNDIERTYNDLTTRLGDPSLASNPQEMLRIVRMRAGLERTVEAYDHWQRL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAERNAVRQMIRDEQDPELRELAEEEHTGLEERIARLEDEITLLLLPRDPNDDKNVMLEI
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEE
RAGTGGDEAAIFAGDLMRMYIRYAEGQGWSVKLASESPAEMGGFKEVILEIRGESVYSKL
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCHHHHHHHHCCHHHHHHH
KFEAGVHRVQRVPVTETQGRVHTSTATVAIMPEVEEVDVEINPNDIEITTTRSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCHHHEEEEECCCEEEEEEECCCCCCCCC
VNKVETAVHLVHKPSGIHIHCQEERSQLQNKARAMQILRVKLYDIKQREQHEQVSGLRRS
CHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGDRSEKIRTYNYKDNRVTDHRLSQNFTLNTAIEGEIETLIQSAIAAAQREQLAELA
CCCCCCCCCCEEEECCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
RSSG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292