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The map label for this gene is rsuA [C]

Identifier: 37523966

GI number: 37523966

Start: 4624801

End: 4625547

Strand: Direct

Name: rsuA [C]

Synonym: glr4397

Alternate gene names: 37523966

Gene position: 4624801-4625547 (Clockwise)

Preceding gene: 37523965

Following gene: 37523967

Centisome position: 99.27

GC content: 63.05

Gene sequence:

>747_bases
ATGGGAGCAGCTATCCGTCTTCAAAAGCTTCTGGCTGAGCACGGTATCGCCTCCCGCCGTAAAGCGGAGGCATTGATCGT
CTCAGGTCAGGTGTGCGTCAACGGCACGGTCGTCGATCGGTTGGGCACTCGGGTCGATCCCCAAATTGATCTCATCGAAG
TGTTCGGCCAGCCGTTGCACTGCATGCCGCCGTTGCTCTATGTCCTTTTGCACAAGCCGGTCGGTTGGCTTTCGACCTGC
TTTGATCCGCGTGGGCGGCGGACGGTACTCGATTTGTTGCCGGCTGAATGGCGGACGGGCCAGGGTCTGCACCCGGTGGG
CCGTCTCGATTGCGACAGTTCGGGGGCATTGATTGTCAGCAACGATGGGGAGTTCACCTTCCGGCTTACCCATCCGCGCC
ACCAGTTGCCTAAAACCTATCGAGTCTGGGTGCGTGGCCGACCGACGGCGGCAGTACTCGGGCGCTGGCGCGCGGGCGTC
GTGCTTGAGGGCCAAAAGACGTTGCCGGCGGCGGTCACGGTCGTGAGCCGGGCTGCTGAGGCGACGGAGCTGGAGGTTGT
CCTGGTCGAAGGCCGCAACCGCCAGATCCGCAAGGTGGCCGAGCTTCTGGGGCATCCGGTCGAGCGGCTGCACCGCACGG
CCATCGGCCCTGTTGCCCTGGCGGGACTGCCCCCGGGCGCCCACCGTTTATTATCCGCTTCTCAAATCGAACGACTACTG
CAAGCCACTGGAACCTTCGACTTATGA

Upstream 100 bases:

>100_bases
GAGGTCCACAACCTGGCGCGGGAATTTGAATGTATCCTGGTTCACACCCCCCCCGAAGATGCCAACAGTTACCAGGTAGG
TTTTTTCAACAGCGGCGGCA

Downstream 100 bases:

>100_bases
CAACCGAGAGTTTTCAACCCGACTCGCTCAAATCGGTGGGCTCATTGCTTGCGAGAGGCCGCCAGCAACGCGGCTGGTCG
GTTGACGACGTCTCGGAGCG

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MGAAIRLQKLLAEHGIASRRKAEALIVSGQVCVNGTVVDRLGTRVDPQIDLIEVFGQPLHCMPPLLYVLLHKPVGWLSTC
FDPRGRRTVLDLLPAEWRTGQGLHPVGRLDCDSSGALIVSNDGEFTFRLTHPRHQLPKTYRVWVRGRPTAAVLGRWRAGV
VLEGQKTLPAAVTVVSRAAEATELEVVLVEGRNRQIRKVAELLGHPVERLHRTAIGPVALAGLPPGAHRLLSASQIERLL
QATGTFDL

Sequences:

>Translated_248_residues
MGAAIRLQKLLAEHGIASRRKAEALIVSGQVCVNGTVVDRLGTRVDPQIDLIEVFGQPLHCMPPLLYVLLHKPVGWLSTC
FDPRGRRTVLDLLPAEWRTGQGLHPVGRLDCDSSGALIVSNDGEFTFRLTHPRHQLPKTYRVWVRGRPTAAVLGRWRAGV
VLEGQKTLPAAVTVVSRAAEATELEVVLVEGRNRQIRKVAELLGHPVERLHRTAIGPVALAGLPPGAHRLLSASQIERLL
QATGTFDL
>Mature_247_residues
GAAIRLQKLLAEHGIASRRKAEALIVSGQVCVNGTVVDRLGTRVDPQIDLIEVFGQPLHCMPPLLYVLLHKPVGWLSTCF
DPRGRRTVLDLLPAEWRTGQGLHPVGRLDCDSSGALIVSNDGEFTFRLTHPRHQLPKTYRVWVRGRPTAAVLGRWRAGVV
LEGQKTLPAAVTVVSRAAEATELEVVLVEGRNRQIRKVAELLGHPVERLHRTAIGPVALAGLPPGAHRLLSASQIERLLQ
ATGTFDL

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=32.4894514767932, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787524, Length=252, Percent_Identity=37.3015873015873, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=32.3651452282158, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=35.3591160220994, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27104; Mature: 26973

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50889 S4 ; PS00070 ALDEHYDE_DEHYDR_CYS ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAIRLQKLLAEHGIASRRKAEALIVSGQVCVNGTVVDRLGTRVDPQIDLIEVFGQPLH
CCHHHHHHHHHHHCCCCCCCCCCEEEEECCEEECCEEHHHCCCCCCCCHHHHHHCCCCCH
CMPPLLYVLLHKPVGWLSTCFDPRGRRTVLDLLPAEWRTGQGLHPVGRLDCDSSGALIVS
HHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
NDGEFTFRLTHPRHQLPKTYRVWVRGRPTAAVLGRWRAGVVLEGQKTLPAAVTVVSRAAE
CCCCEEEEECCCHHHCCCEEEEEECCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCC
ATELEVVLVEGRNRQIRKVAELLGHPVERLHRTAIGPVALAGLPPGAHRLLSASQIERLL
CCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
QATGTFDL
HHCCCCCC
>Mature Secondary Structure 
GAAIRLQKLLAEHGIASRRKAEALIVSGQVCVNGTVVDRLGTRVDPQIDLIEVFGQPLH
CHHHHHHHHHHHCCCCCCCCCCEEEEECCEEECCEEHHHCCCCCCCCHHHHHHCCCCCH
CMPPLLYVLLHKPVGWLSTCFDPRGRRTVLDLLPAEWRTGQGLHPVGRLDCDSSGALIVS
HHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
NDGEFTFRLTHPRHQLPKTYRVWVRGRPTAAVLGRWRAGVVLEGQKTLPAAVTVVSRAAE
CCCCEEEEECCCHHHCCCEEEEEECCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCC
ATELEVVLVEGRNRQIRKVAELLGHPVERLHRTAIGPVALAGLPPGAHRLLSASQIERLL
CCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
QATGTFDL
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]