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The map label for this gene is rsmE [H]

Identifier: 37523950

GI number: 37523950

Start: 4612111

End: 4612827

Strand: Reverse

Name: rsmE [H]

Synonym: gll4381

Alternate gene names: 37523950

Gene position: 4612827-4612111 (Counterclockwise)

Preceding gene: 37523951

Following gene: 37523949

Centisome position: 99.01

GC content: 66.25

Gene sequence:

>717_bases
ATGGGCAGCACCGCCCGGCTGTTTCTGGACCCGGTTCAGTTTAGCGGTGATACCGCCCGGCTCGACCCGGCCCAGTCGCA
TTACCTCAGGCAGGTACTGCGGCTGCGGGACGGAGCAAGTTGCATCGTAGGCGACGGCCTGGGCAATACCTGGCAGGCGA
CCCTCGCGGGCGACACCGTCAGGCTCGGCTCGCTTTTGCCCCGAACGGGCGAATTGCCCCTGCCGGTCACCATCGCCTGC
GCGGTTCCCAAGGGCGATCGCTGGGACTGGCTGTTGCAGAAGTCTACAGAGCTGGGGGCAGACCGGATTGTGCCGCTGGT
GTGCGAGCGCTCGGTGGTGCAGCCGGATGCCAAGCGGCACAAGCGCTGGCAGGCGATCGTCCGCGAAGCCGCGGAGCAGT
CGGAGCGCGCCGTGCTGCCCGTCGTCGAACCACCGGCCTTGTTTGGCATGTTTCTGAGGCACCCTGCCCGGGGTACGCGG
CTTATCTGCACTGCCCGCGGTGTGCGGCCACCTTTGTTGAATTATTTGCCGGCCGCCGCCCTGACATTGCTATTTGGGCC
GGAAGGGGGGTTCAGTGAGACCGAAGTGGCCGCCGCCACCGACGCCGGTTACCAGGTATGCAGCCTGGGCAGCCGTATCC
TGCGCACCGAGACCGCACCGTTGCTTGCCCTCGGTTGGATTGCCGCCTGGTACGAAGGGAAGCCTGAGGACACTTGA

Upstream 100 bases:

>100_bases
GCGACGAATTGTTGGGCGGCGGCTTCAAGTTCAACAACCCCAACGCCGAGCGCTCCTGCTCCTGCGGCAGTTCCTTCTCG
GCCTAGCGGCGCACCGCCCC

Downstream 100 bases:

>100_bases
CGCTCCCTGGCGCAAATGCCACGATCAAATTACAAGATGTTACGCACAAGCCGCCAAGGATGCCATGCTTGTCCCTTTCG
ACCCTTCGCAATTCGAATCC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MGSTARLFLDPVQFSGDTARLDPAQSHYLRQVLRLRDGASCIVGDGLGNTWQATLAGDTVRLGSLLPRTGELPLPVTIAC
AVPKGDRWDWLLQKSTELGADRIVPLVCERSVVQPDAKRHKRWQAIVREAAEQSERAVLPVVEPPALFGMFLRHPARGTR
LICTARGVRPPLLNYLPAAALTLLFGPEGGFSETEVAAATDAGYQVCSLGSRILRTETAPLLALGWIAAWYEGKPEDT

Sequences:

>Translated_238_residues
MGSTARLFLDPVQFSGDTARLDPAQSHYLRQVLRLRDGASCIVGDGLGNTWQATLAGDTVRLGSLLPRTGELPLPVTIAC
AVPKGDRWDWLLQKSTELGADRIVPLVCERSVVQPDAKRHKRWQAIVREAAEQSERAVLPVVEPPALFGMFLRHPARGTR
LICTARGVRPPLLNYLPAAALTLLFGPEGGFSETEVAAATDAGYQVCSLGSRILRTETAPLLALGWIAAWYEGKPEDT
>Mature_237_residues
GSTARLFLDPVQFSGDTARLDPAQSHYLRQVLRLRDGASCIVGDGLGNTWQATLAGDTVRLGSLLPRTGELPLPVTIACA
VPKGDRWDWLLQKSTELGADRIVPLVCERSVVQPDAKRHKRWQAIVREAAEQSERAVLPVVEPPALFGMFLRHPARGTRL
ICTARGVRPPLLNYLPAAALTLLFGPEGGFSETEVAAATDAGYQVCSLGSRILRTETAPLLALGWIAAWYEGKPEDT

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=231, Percent_Identity=27.7056277056277, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25833; Mature: 25702

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSTARLFLDPVQFSGDTARLDPAQSHYLRQVLRLRDGASCIVGDGLGNTWQATLAGDTV
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCEE
RLGSLLPRTGELPLPVTIACAVPKGDRWDWLLQKSTELGADRIVPLVCERSVVQPDAKRH
EHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHH
KRWQAIVREAAEQSERAVLPVVEPPALFGMFLRHPARGTRLICTARGVRPPLLNYLPAAA
HHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
LTLLFGPEGGFSETEVAAATDAGYQVCSLGSRILRTETAPLLALGWIAAWYEGKPEDT
HEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GSTARLFLDPVQFSGDTARLDPAQSHYLRQVLRLRDGASCIVGDGLGNTWQATLAGDTV
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCEE
RLGSLLPRTGELPLPVTIACAVPKGDRWDWLLQKSTELGADRIVPLVCERSVVQPDAKRH
EHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHH
KRWQAIVREAAEQSERAVLPVVEPPALFGMFLRHPARGTRLICTARGVRPPLLNYLPAAA
HHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
LTLLFGPEGGFSETEVAAATDAGYQVCSLGSRILRTETAPLLALGWIAAWYEGKPEDT
HEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]