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The map label for this gene is rlmI [H]

Identifier: 37523934

GI number: 37523934

Start: 4599386

End: 4600609

Strand: Direct

Name: rlmI [H]

Synonym: glr4365

Alternate gene names: 37523934

Gene position: 4599386-4600609 (Clockwise)

Preceding gene: 37523933

Following gene: 37523935

Centisome position: 98.72

GC content: 65.36

Gene sequence:

>1224_bases
GTGCGGAACCGTGTTGCGCGCCGGGAGGTTGGGGTGAGTGCGCCCGGGGCGGTGCAGCTGAAGCCCCAACGCCGCAACCG
CCGCACCCATCCGTGGATTTTTGCGAGTGAAGTCGCCCGTTTTCCACCGCTTGAGCAGGTGGCGGACGGCAGCATGGTCG
ATGTGCTCGATGCCCAGGGCCGCTTTTGCGGGCGGGGATTTCTCAACCGTCAATCACAAATCGCGGTGCGCTATCTGGAG
CGCGGCGCGGATGTGGTAATCGACCGGGCCTGGTGGATCGAACGGTTCGGTAAGGCCCTGGAGCACCGGCACCGTTTTTT
GGGGCCGCAGGTGAAGACTTTCCGCTGGATCCACGGCGAGGCGGACGGCCTGCCGGGGCTCACGGTCGATCGCTACGGCG
ATTTTGTCGTCGTGCAGCTGTTGGCGCTGGGGCTTGAGCCCTGGCGCGAAGTGATCGTCGAGTGTCTGGCGCAACTGGGG
TCGCCTCTGGGCATCTTCGAGCGCTCCGACGCACCGGTGCGCCGCCTGGAGGGGCTCAAGGAGCGGGTGGGTTGTTTGTG
GGGCGAGGCGCCGCCGACGCTGGTTGAGATCGAAGAAGGTTCCGCCCGGCTCCTGGTGGACATCCAGCAGGGGCAAAAGA
CGGGCCTTTTTCTCGATCAGCGCTTCAACCGCCAGCACGCCGCCCGCTTTGCCGAGGGGCGCAACGTGCTCAACGCCTTC
GGCTACACCGGTGCTTTTGGGGTGCAATGCGCCCTGGCCGGGGCCAGTTCGGTCCTCAACGTCGATATCGCCGAGGCGGC
CACCGCGATCGCCGAGCAAAACGCCCAGCTCAATGGGGTGGGCGAGCAGTGCCGGGCGCTCACCGCCAACGCCTTCGATG
TATTGCGCGAATTCGACCGGGAGGGTCGGCGCTTCGAGATGATTATTCTCGATCCGCCCGCCTTCGCCAAAAGCCGCAGC
GTGCTGGAGGGGGCTCTGCGCGGCTACAAGGAGATCAACCTCCGGGCAATGAAGCTGCTCACCCCAGGGGGGATCCTGGT
CACCTGCTCGTGCTCGTCGCACCTGAGCATGGAGATGTTCCGCGACGTCATCCGCGACGCCGCCCAGGATACCGGCCGCA
CCGCCTGTCTGGTCGAACAGCGCGGCCAGGCGCCCGATCACCCGATCTTGCTGCATATCCCCGAGACCGAGTACCTGAAA
TATCTTCTGATCAGCATCGACTGA

Upstream 100 bases:

>100_bases
CGAACCCGGCCGCCGCTGACAAAACTGTTGTTTCGTCTGTATTATTCCCGCAGAGGGGTTGTCTAATGTTTCAGCAACGT
ATAGCAGTTTTTTCCTCCAG

Downstream 100 bases:

>100_bases
GGCGGGGCACGATAGGGATAAAAGCGTTCCGGGGGGCGATGAAAATCGAGATCAAGTTGTTCGCCAGCTACCGCGAGGCG
TTCGAGCGCGACGAGTTGGT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MRNRVARREVGVSAPGAVQLKPQRRNRRTHPWIFASEVARFPPLEQVADGSMVDVLDAQGRFCGRGFLNRQSQIAVRYLE
RGADVVIDRAWWIERFGKALEHRHRFLGPQVKTFRWIHGEADGLPGLTVDRYGDFVVVQLLALGLEPWREVIVECLAQLG
SPLGIFERSDAPVRRLEGLKERVGCLWGEAPPTLVEIEEGSARLLVDIQQGQKTGLFLDQRFNRQHAARFAEGRNVLNAF
GYTGAFGVQCALAGASSVLNVDIAEAATAIAEQNAQLNGVGEQCRALTANAFDVLREFDREGRRFEMIILDPPAFAKSRS
VLEGALRGYKEINLRAMKLLTPGGILVTCSCSSHLSMEMFRDVIRDAAQDTGRTACLVEQRGQAPDHPILLHIPETEYLK
YLLISID

Sequences:

>Translated_407_residues
MRNRVARREVGVSAPGAVQLKPQRRNRRTHPWIFASEVARFPPLEQVADGSMVDVLDAQGRFCGRGFLNRQSQIAVRYLE
RGADVVIDRAWWIERFGKALEHRHRFLGPQVKTFRWIHGEADGLPGLTVDRYGDFVVVQLLALGLEPWREVIVECLAQLG
SPLGIFERSDAPVRRLEGLKERVGCLWGEAPPTLVEIEEGSARLLVDIQQGQKTGLFLDQRFNRQHAARFAEGRNVLNAF
GYTGAFGVQCALAGASSVLNVDIAEAATAIAEQNAQLNGVGEQCRALTANAFDVLREFDREGRRFEMIILDPPAFAKSRS
VLEGALRGYKEINLRAMKLLTPGGILVTCSCSSHLSMEMFRDVIRDAAQDTGRTACLVEQRGQAPDHPILLHIPETEYLK
YLLISID
>Mature_407_residues
MRNRVARREVGVSAPGAVQLKPQRRNRRTHPWIFASEVARFPPLEQVADGSMVDVLDAQGRFCGRGFLNRQSQIAVRYLE
RGADVVIDRAWWIERFGKALEHRHRFLGPQVKTFRWIHGEADGLPGLTVDRYGDFVVVQLLALGLEPWREVIVECLAQLG
SPLGIFERSDAPVRRLEGLKERVGCLWGEAPPTLVEIEEGSARLLVDIQQGQKTGLFLDQRFNRQHAARFAEGRNVLNAF
GYTGAFGVQCALAGASSVLNVDIAEAATAIAEQNAQLNGVGEQCRALTANAFDVLREFDREGRRFEMIILDPPAFAKSRS
VLEGALRGYKEINLRAMKLLTPGGILVTCSCSSHLSMEMFRDVIRDAAQDTGRTACLVEQRGQAPDHPILLHIPETEYLK
YLLISID

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=375, Percent_Identity=41.0666666666667, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787181, Length=247, Percent_Identity=31.1740890688259, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45365; Mature: 45365

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRVARREVGVSAPGAVQLKPQRRNRRTHPWIFASEVARFPPLEQVADGSMVDVLDAQG
CCCCHHHHHCCCCCCCEEEECCHHCCCCCCCEEEHHHHHCCCCHHHHCCCCEEEECCCCC
RFCGRGFLNRQSQIAVRYLERGADVVIDRAWWIERFGKALEHRHRFLGPQVKTFRWIHGE
CHHCCHHCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
ADGLPGLTVDRYGDFVVVQLLALGLEPWREVIVECLAQLGSPLGIFERSDAPVRRLEGLK
CCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHH
ERVGCLWGEAPPTLVEIEEGSARLLVDIQQGQKTGLFLDQRFNRQHAARFAEGRNVLNAF
HHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHC
GYTGAFGVQCALAGASSVLNVDIAEAATAIAEQNAQLNGVGEQCRALTANAFDVLREFDR
CCCCCHHHHEEECCCCHHEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EGRRFEMIILDPPAFAKSRSVLEGALRGYKEINLRAMKLLTPGGILVTCSCSSHLSMEMF
CCCEEEEEEECCCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCCEEEEECCCCCCCHHHH
RDVIRDAAQDTGRTACLVEQRGQAPDHPILLHIPETEYLKYLLISID
HHHHHHHHHHCCCEEEEEHHCCCCCCCCEEEECCCHHHEEEEEEECC
>Mature Secondary Structure
MRNRVARREVGVSAPGAVQLKPQRRNRRTHPWIFASEVARFPPLEQVADGSMVDVLDAQG
CCCCHHHHHCCCCCCCEEEECCHHCCCCCCCEEEHHHHHCCCCHHHHCCCCEEEECCCCC
RFCGRGFLNRQSQIAVRYLERGADVVIDRAWWIERFGKALEHRHRFLGPQVKTFRWIHGE
CHHCCHHCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
ADGLPGLTVDRYGDFVVVQLLALGLEPWREVIVECLAQLGSPLGIFERSDAPVRRLEGLK
CCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHH
ERVGCLWGEAPPTLVEIEEGSARLLVDIQQGQKTGLFLDQRFNRQHAARFAEGRNVLNAF
HHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHC
GYTGAFGVQCALAGASSVLNVDIAEAATAIAEQNAQLNGVGEQCRALTANAFDVLREFDR
CCCCCHHHHEEECCCCHHEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EGRRFEMIILDPPAFAKSRSVLEGALRGYKEINLRAMKLLTPGGILVTCSCSSHLSMEMF
CCCEEEEEEECCCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCCEEEEECCCCCCCHHHH
RDVIRDAAQDTGRTACLVEQRGQAPDHPILLHIPETEYLKYLLISID
HHHHHHHHHHCCCEEEEEHHCCCCCCCCEEEECCCHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA