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The map label for this gene is rimO

Identifier: 37523886

GI number: 37523886

Start: 4549438

End: 4550751

Strand: Reverse

Name: rimO

Synonym: gll4317

Alternate gene names: 37523886

Gene position: 4550751-4549438 (Counterclockwise)

Preceding gene: 37523888

Following gene: 37523885

Centisome position: 97.68

GC content: 64.16

Gene sequence:

>1314_bases
ATGCCAGCCACGATCGCCGTCACCCACTTGGGCTGCGAGAAAAACCGCATCGACACCGAACACATGCTGGGGCTTCTGGC
CCAGGCCGGTTACCAGATTGATTCCGACGAGGACAAAGCCGAGTACGTCCTGGTCAACACCTGCAGCTTCATCGGCCCGG
CGCGCACCGAGTCGGTGCGCACCCTCGTCAACCTGGCGGAGCAGGGCAAGAAGATTATCATCGCCGGCTGCCTGCCCCAG
ATGTTCCGCGACGAATTGCTCAAAGAGATTCCCGAAGCGGTGGCTATCGTCGGCACCGGCGACTACCACCGCATCGTCGA
GGTGGTCGAGCGCAGCCGTACAGGGGAACGGGTCAGCCTGGTGAGCGCCACACCGACGTACATCGCCGACGAGCATCTAC
CGCGCTACCGCACCACCACCGAGGCGGTGGCCTACCTCAAGATTGCCGAAGGCTGCGACTACCGCTGCGCGTTTTGCATA
ATTCCCCACCTGCGGGGCGACCAGCGCAGCCGCCCGATCGAATCGATCGTGGCCGAAGCGAAGCAACTGGCGAGCGAAGG
GGTCAAAGAACTGATCTTGATTTCCCAAATCTCGACCAACTACGGCCTCGACCTCTACGGCAAACCCCGCCTCGCGGACC
TGCTGCGCGCCCTTGGGGAAGTGGACATCCCCTGGGTACGGGTGCACTACGCCTACCCGACCGGCCTCACCGACGAATTG
CTTACTGCCGTCGAGCAGACCGCCAACGTGCTGCCCTACTTCGATGTGCCCCTCCAGCACAGCCACCCGGAGGTGCTGAG
GGCGATGAATCGCCCCTGGCAGGCCGACCTTAACACCCGGCTGTTGGAGCGTATCCGCGAGCGCCTTCCCGAAGCGACCC
TGCGCACGACGCTCATCGTCGGCTTTCCCGGCGAGAGCGAGGCGCACTTTGCCCATCTGTGCGCCTTTGTCGAGCGCTCC
GAGTTCGATCACGTGGGCGTGTTCGCCTACTCGCGCGAAGAGAACACCGCCGCCGCCAACCTGGAGGGCCAGATCCCCGA
AGCGATCAAAAAGCGCCGCCGCCGCGATCTGATGCGCCTGCAGCAGACGATTTCCCAGCGCCGCAACGCCTCGCAGGTGG
GCCGGGTGGTGCCGGTGCTCATCGAGCAGGAGAATACCCAGAAAAAAGTATGGCTCGGCCGTTCGGCACGCTTTTCCCCG
GAGATCGACGGTCAGGTGATCGTCTGCGGCGGGGCCGCCCTCAACCAGTTGATCCCCGTGCGCATCACCGCCGCCACGCC
CTACGATCTCTGCGGCGAAGTGTTCCAAGCCTGA

Upstream 100 bases:

>100_bases
TCGCCTGCCACCAGTCTGACAGAGCCCACGCCCGGCGGCGCATCCGAAGCTCGCGATCCCCCATGCCATAATGAAGTGTT
TGCAAGCATTCACCATTTCT

Downstream 100 bases:

>100_bases
TACAAAAAGTTCTCCAAGAACACCCGGGATCGCTAGGGCGTGCTCTGATGGAGACTAAGGCGAGCACTATCGCCTGGATA
AGTTGTCCTTATTCGAACCC

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPATIAVTHLGCEKNRIDTEHMLGLLAQAGYQIDSDEDKAEYVLVNTCSFIGPARTESVRTLVNLAEQGKKIIIAGCLPQ
MFRDELLKEIPEAVAIVGTGDYHRIVEVVERSRTGERVSLVSATPTYIADEHLPRYRTTTEAVAYLKIAEGCDYRCAFCI
IPHLRGDQRSRPIESIVAEAKQLASEGVKELILISQISTNYGLDLYGKPRLADLLRALGEVDIPWVRVHYAYPTGLTDEL
LTAVEQTANVLPYFDVPLQHSHPEVLRAMNRPWQADLNTRLLERIRERLPEATLRTTLIVGFPGESEAHFAHLCAFVERS
EFDHVGVFAYSREENTAAANLEGQIPEAIKKRRRRDLMRLQQTISQRRNASQVGRVVPVLIEQENTQKKVWLGRSARFSP
EIDGQVIVCGGAALNQLIPVRITAATPYDLCGEVFQA

Sequences:

>Translated_437_residues
MPATIAVTHLGCEKNRIDTEHMLGLLAQAGYQIDSDEDKAEYVLVNTCSFIGPARTESVRTLVNLAEQGKKIIIAGCLPQ
MFRDELLKEIPEAVAIVGTGDYHRIVEVVERSRTGERVSLVSATPTYIADEHLPRYRTTTEAVAYLKIAEGCDYRCAFCI
IPHLRGDQRSRPIESIVAEAKQLASEGVKELILISQISTNYGLDLYGKPRLADLLRALGEVDIPWVRVHYAYPTGLTDEL
LTAVEQTANVLPYFDVPLQHSHPEVLRAMNRPWQADLNTRLLERIRERLPEATLRTTLIVGFPGESEAHFAHLCAFVERS
EFDHVGVFAYSREENTAAANLEGQIPEAIKKRRRRDLMRLQQTISQRRNASQVGRVVPVLIEQENTQKKVWLGRSARFSP
EIDGQVIVCGGAALNQLIPVRITAATPYDLCGEVFQA
>Mature_436_residues
PATIAVTHLGCEKNRIDTEHMLGLLAQAGYQIDSDEDKAEYVLVNTCSFIGPARTESVRTLVNLAEQGKKIIIAGCLPQM
FRDELLKEIPEAVAIVGTGDYHRIVEVVERSRTGERVSLVSATPTYIADEHLPRYRTTTEAVAYLKIAEGCDYRCAFCII
PHLRGDQRSRPIESIVAEAKQLASEGVKELILISQISTNYGLDLYGKPRLADLLRALGEVDIPWVRVHYAYPTGLTDELL
TAVEQTANVLPYFDVPLQHSHPEVLRAMNRPWQADLNTRLLERIRERLPEATLRTTLIVGFPGESEAHFAHLCAFVERSE
FDHVGVFAYSREENTAAANLEGQIPEAIKKRRRRDLMRLQQTISQRRNASQVGRVVPVLIEQENTQKKVWLGRSARFSPE
IDGQVIVCGGAALNQLIPVRITAATPYDLCGEVFQA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=441, Percent_Identity=29.0249433106576, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI28872784, Length=414, Percent_Identity=27.7777777777778, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI28872782, Length=414, Percent_Identity=27.7777777777778, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=36.6292134831461, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=28.0623608017817, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=440, Percent_Identity=27.2727272727273, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71996771, Length=367, Percent_Identity=26.158038147139, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356207, Length=429, Percent_Identity=27.5058275058275, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922432, Length=453, Percent_Identity=28.9183222958057, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_GLOVI (Q7NDB8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927263.1
- ProteinModelPortal:   Q7NDB8
- SMR:   Q7NDB8
- GeneID:   2602720
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll4317
- NMPDR:   fig|251221.1.peg.4317
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK893584
- BioCyc:   GVIO251221:GLL4317-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48877; Mature: 48745

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATIAVTHLGCEKNRIDTEHMLGLLAQAGYQIDSDEDKAEYVLVNTCSFIGPARTESVR
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHH
TLVNLAEQGKKIIIAGCLPQMFRDELLKEIPEAVAIVGTGDYHRIVEVVERSRTGERVSL
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCEEEE
VSATPTYIADEHLPRYRTTTEAVAYLKIAEGCDYRCAFCIIPHLRGDQRSRPIESIVAEA
EECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
KQLASEGVKELILISQISTNYGLDLYGKPRLADLLRALGEVDIPWVRVHYAYPTGLTDEL
HHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
LTAVEQTANVLPYFDVPLQHSHPEVLRAMNRPWQADLNTRLLERIRERLPEATLRTTLIV
HHHHHHHHCCCCEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEEE
GFPGESEAHFAHLCAFVERSEFDHVGVFAYSREENTAAANLEGQIPEAIKKRRRRDLMRL
ECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHH
QQTISQRRNASQVGRVVPVLIEQENTQKKVWLGRSARFSPEIDGQVIVCGGAALNQLIPV
HHHHHHHCCHHHHHCEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCHHHCCEEEE
RITAATPYDLCGEVFQA
EEEECCCHHHHHHHHCC
>Mature Secondary Structure 
PATIAVTHLGCEKNRIDTEHMLGLLAQAGYQIDSDEDKAEYVLVNTCSFIGPARTESVR
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHH
TLVNLAEQGKKIIIAGCLPQMFRDELLKEIPEAVAIVGTGDYHRIVEVVERSRTGERVSL
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCEEEE
VSATPTYIADEHLPRYRTTTEAVAYLKIAEGCDYRCAFCIIPHLRGDQRSRPIESIVAEA
EECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
KQLASEGVKELILISQISTNYGLDLYGKPRLADLLRALGEVDIPWVRVHYAYPTGLTDEL
HHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
LTAVEQTANVLPYFDVPLQHSHPEVLRAMNRPWQADLNTRLLERIRERLPEATLRTTLIV
HHHHHHHHCCCCEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEEE
GFPGESEAHFAHLCAFVERSEFDHVGVFAYSREENTAAANLEGQIPEAIKKRRRRDLMRL
ECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHH
QQTISQRRNASQVGRVVPVLIEQENTQKKVWLGRSARFSPEIDGQVIVCGGAALNQLIPV
HHHHHHHCCHHHHHCEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCHHHCCEEEE
RITAATPYDLCGEVFQA
EEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292