Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 37523883

GI number: 37523883

Start: 4547221

End: 4547985

Strand: Reverse

Name: hisF

Synonym: gvip560

Alternate gene names: 37523883

Gene position: 4547985-4547221 (Counterclockwise)

Preceding gene: 37523885

Following gene: 37523882

Centisome position: 97.62

GC content: 70.2

Gene sequence:

>765_bases
ATGCTGGCAAAACGCATCGTTCCCTGCCTCGACGTCAAGGCGGGCAGGGTGGTCAAGGGGGTCAACTTCGTCGGGCTGCG
CGACGCGGGCGACCCGGTGGAACTGGCGAAGCTCTACAACGACCAGGGCGCAGACGAACTGGTGTTCCTGGATATTGCCG
CCACCCACGAAGAGCGGGCAATCCTCATCGACGTGGTCTACCGGACCGCCGAGCAGGTGTTCATCCCCCTGACCGTGGGC
GGCGGCATCGGCGATCTGGACACGATTCAGGCCTTGCTCGACGCCGGGGCCGACAAAGTGAGCATCAATTCCGCCGCCGT
GCGCGACCCCGAACTGATTGCGCGCGCCGGCGAGCGCTTTGGTGCGCAGTGCATCGTGCTTGCCATCGACGCGCGCGCCC
GCGCAGGCGGCGCGGGCTGGGAGGTCTACGTGCGCGGCGGCCGTACCCCCACCGGCCTCGACGCGGTGGCCTGGGCCGTC
GAGGGCGAGCGGCGGGGGGCGGGGGAGATTTTGCTCACCAGCATGGACGCGGACGGCACCCGGGCCGGTTACGACCTGGC
CCTCACCCGGGCGGTGGCCGAGGCGGTGCAGCTGCCGGTGATCGCCTCGGGGGGGGCGGGCAACTGCGAACACATCCGCA
CCGCCCTCACCGAGGGGGCGGCGCAGGCGGCATTGCTCGCTTCGCTGTTGCACTACGGCGAACTCACCGTGGAGCAGATC
AAAGCGCACCTCGCCGCGCACGGCGTGCCCGTGCGACGGGGTTGA

Upstream 100 bases:

>100_bases
GCGTATACCACGTAGGTTCGGCACGGCCCGGCTCCTGGACGAAAATCTTCAAATTTTTTCATAATGGTACCATTACCGGG
CACATTCAGGCCGCGCATCC

Downstream 100 bases:

>100_bases
TGTCGTCGGTGCTGCTGCGCAATCTGCCCATCGCCGTGGGCCTCCTGGGGGGCAGTCTGGTGGTGCTCAACCGGCTGTTG
GGTACCGCGAACCTCGCCCC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLAKRIVPCLDVKAGRVVKGVNFVGLRDAGDPVELAKLYNDQGADELVFLDIAATHEERAILIDVVYRTAEQVFIPLTVG
GGIGDLDTIQALLDAGADKVSINSAAVRDPELIARAGERFGAQCIVLAIDARARAGGAGWEVYVRGGRTPTGLDAVAWAV
EGERRGAGEILLTSMDADGTRAGYDLALTRAVAEAVQLPVIASGGAGNCEHIRTALTEGAAQAALLASLLHYGELTVEQI
KAHLAAHGVPVRRG

Sequences:

>Translated_254_residues
MLAKRIVPCLDVKAGRVVKGVNFVGLRDAGDPVELAKLYNDQGADELVFLDIAATHEERAILIDVVYRTAEQVFIPLTVG
GGIGDLDTIQALLDAGADKVSINSAAVRDPELIARAGERFGAQCIVLAIDARARAGGAGWEVYVRGGRTPTGLDAVAWAV
EGERRGAGEILLTSMDADGTRAGYDLALTRAVAEAVQLPVIASGGAGNCEHIRTALTEGAAQAALLASLLHYGELTVEQI
KAHLAAHGVPVRRG
>Mature_254_residues
MLAKRIVPCLDVKAGRVVKGVNFVGLRDAGDPVELAKLYNDQGADELVFLDIAATHEERAILIDVVYRTAEQVFIPLTVG
GGIGDLDTIQALLDAGADKVSINSAAVRDPELIARAGERFGAQCIVLAIDARARAGGAGWEVYVRGGRTPTGLDAVAWAV
EGERRGAGEILLTSMDADGTRAGYDLALTRAVAEAVQLPVIASGGAGNCEHIRTALTEGAAQAALLASLLHYGELTVEQI
KAHLAAHGVPVRRG

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=46.484375, Blast_Score=210, Evalue=6e-56,
Organism=Escherichia coli, GI87082028, Length=238, Percent_Identity=29.8319327731092, Blast_Score=80, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=34.8242811501597, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_GLOVI (Q7NDC1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927260.1
- ProteinModelPortal:   Q7NDC1
- SMR:   Q7NDC1
- GeneID:   2602717
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.4314
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   GVIO251221:GLL4314-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26493; Mature: 26493

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIVPCLDVKAGRVVKGVNFVGLRDAGDPVELAKLYNDQGADELVFLDIAATHEERA
CCHHHCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCE
ILIDVVYRTAEQVFIPLTVGGGIGDLDTIQALLDAGADKVSINSAAVRDPELIARAGERF
EEEHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GAQCIVLAIDARARAGGAGWEVYVRGGRTPTGLDAVAWAVEGERRGAGEILLTSMDADGT
CCEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHEEEEECCCCCCCCEEEEEECCCCCC
RAGYDLALTRAVAEAVQLPVIASGGAGNCEHIRTALTEGAAQAALLASLLHYGELTVEQI
CCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KAHLAAHGVPVRRG
HHHHHHCCCCCCCC
>Mature Secondary Structure
MLAKRIVPCLDVKAGRVVKGVNFVGLRDAGDPVELAKLYNDQGADELVFLDIAATHEERA
CCHHHCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCE
ILIDVVYRTAEQVFIPLTVGGGIGDLDTIQALLDAGADKVSINSAAVRDPELIARAGERF
EEEHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHC
GAQCIVLAIDARARAGGAGWEVYVRGGRTPTGLDAVAWAVEGERRGAGEILLTSMDADGT
CCEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHEEEEECCCCCCCCEEEEEECCCCCC
RAGYDLALTRAVAEAVQLPVIASGGAGNCEHIRTALTEGAAQAALLASLLHYGELTVEQI
CCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KAHLAAHGVPVRRG
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292