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The map label for this gene is tig

Identifier: 37523764

GI number: 37523764

Start: 4390674

End: 4392059

Strand: Direct

Name: tig

Synonym: glr4195

Alternate gene names: 37523764

Gene position: 4390674-4392059 (Clockwise)

Preceding gene: 37523761

Following gene: 37523765

Centisome position: 94.24

GC content: 62.12

Gene sequence:

>1386_bases
ATGAAAGTCACGCAAGAGAAGCTCCCGCGCTCCCAGATGGGCCTCAATGTCGAGGTCGAAGGCGAAAAATCCAAGCAAGC
TTACGAGAAACTGGTGCGCGACACCATGCGCACCGCCCGCATCCCCGGTTTTCGCCCCGGCAAAGCCCCGCGCCAGCTGG
TGCTGCAGTTCTACGGCAAGGAGCGCCTCAGGGCGCAGGCGCTCGAAAATCTGATCGACAGCTCCCTCAAAGAGGCGATT
GAACAGGAGTCGATTGCTTCGTTGGGCAACCTGCAACTGCGCGATTCGTTCGAGGAGTTGCTCGGCCGCTACCAGCCGGG
AGAACCGCTCAGCTTCAAAGCCGCCGTCGATGTGCAACCGGAGGTCCAGCTGGGCACCTACACCGGCCTGACGGTGCGCT
ACAGCGAAGTGCCCTACGAGGCCAAGCAGGTAGATGATCAGCTCGAGCAGTACCGCGAGCAACGCGCCGTGCTCGTCCCC
GTCGAGGGTCGGGCGGCCGAGGTCGGCGACACCGCTGTGATCGACTTTGCGGGCACCAAGGCGGCGGACGGCAACGAGAT
CGTCGGGGGTAAAGCCACCGATTTCGAAGTCGAACTGCTGCCGGGTCGGCTCATCGCCGGCTTCACCGAGGGAATCATCG
GTATGCAGATTGGTGAGAGCACCGAGCTTGCATTGCGCTTTCCCGACGATTACCCGCAGCAGGAACTGGCGGGGGTCGAT
GCCAAATTCGCCGTCAGTCTCAAAGATCTCAAGATCAAAGAGCTGCCCGTCCTCGACGACGATTTTGCCGGTGACATCAG
CGAATTTGAGACTCTCGAAGCACTGCGCGCATTTCTGGAGCAGCAGCAGCAGGAGCAGGCGGCGGAGAAGACCCGCGCCA
ACCGCGACGCCGCCATCATCAAGGCGCTGGTGGCCGAGACGACCGTCGACTTACCTGAGACGCTGGTCAACCGCGAAGTG
CAGTTTCTAGCCGAGCAGAGCTTTCGCAACCTCCAACAGCAGGGCATCGACCCCAGCCGCATCTTCACCGAGGAGAACAT
GCCCCGGGTACGCGAAACGCTCAGAGTTGACGCCGAAAATCGCCTCAAGCGCACCCTGGCCCTGGCGCAAGTGGCCCGCG
CCGAAAATATCGTGGTCGAAGAGGAGCAGGTGGCTGCGCGCATCGTCGAGTTGCGCAGCGAACTGGAGGAGGAAGTCTCC
GAGCAGGCCCTGGCCGAATTTGCGCGCGAAGAAATGCTTACGGAGAAGATTTTGGAGTGGCTCGCGGAGCACTCTACAAT
AGAGTTGACTTTGCCGGGTGAGGCGATTGAGCCGGGCTCGGGCGAAGACGCTCCCCCCGAAGTTGCCGCCGGGGCCACCG
AGCCCGAGGCGCAACCCAACTCCTGA

Upstream 100 bases:

>100_bases
ACTCCGGCCTTTGCGCCGGGGAGCGTGTACAATTAGCCTCTGGCTGGTCGCCCATGAATCCGGCTAAGATCATCGTCCGC
TTTTGTTACCTGCCATTTCG

Downstream 100 bases:

>100_bases
AACCTCGTTTCCTCAACACCCAGGCTGCCCCATGCCCAAGTACGACTCCCATCTCAAATGCTCCTTCTGCGGCAAGTCCC
AGGAGCAGGTTCGAAAGCTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKVTQEKLPRSQMGLNVEVEGEKSKQAYEKLVRDTMRTARIPGFRPGKAPRQLVLQFYGKERLRAQALENLIDSSLKEAI
EQESIASLGNLQLRDSFEELLGRYQPGEPLSFKAAVDVQPEVQLGTYTGLTVRYSEVPYEAKQVDDQLEQYREQRAVLVP
VEGRAAEVGDTAVIDFAGTKAADGNEIVGGKATDFEVELLPGRLIAGFTEGIIGMQIGESTELALRFPDDYPQQELAGVD
AKFAVSLKDLKIKELPVLDDDFAGDISEFETLEALRAFLEQQQQEQAAEKTRANRDAAIIKALVAETTVDLPETLVNREV
QFLAEQSFRNLQQQGIDPSRIFTEENMPRVRETLRVDAENRLKRTLALAQVARAENIVVEEEQVAARIVELRSELEEEVS
EQALAEFAREEMLTEKILEWLAEHSTIELTLPGEAIEPGSGEDAPPEVAAGATEPEAQPNS

Sequences:

>Translated_461_residues
MKVTQEKLPRSQMGLNVEVEGEKSKQAYEKLVRDTMRTARIPGFRPGKAPRQLVLQFYGKERLRAQALENLIDSSLKEAI
EQESIASLGNLQLRDSFEELLGRYQPGEPLSFKAAVDVQPEVQLGTYTGLTVRYSEVPYEAKQVDDQLEQYREQRAVLVP
VEGRAAEVGDTAVIDFAGTKAADGNEIVGGKATDFEVELLPGRLIAGFTEGIIGMQIGESTELALRFPDDYPQQELAGVD
AKFAVSLKDLKIKELPVLDDDFAGDISEFETLEALRAFLEQQQQEQAAEKTRANRDAAIIKALVAETTVDLPETLVNREV
QFLAEQSFRNLQQQGIDPSRIFTEENMPRVRETLRVDAENRLKRTLALAQVARAENIVVEEEQVAARIVELRSELEEEVS
EQALAEFAREEMLTEKILEWLAEHSTIELTLPGEAIEPGSGEDAPPEVAAGATEPEAQPNS
>Mature_461_residues
MKVTQEKLPRSQMGLNVEVEGEKSKQAYEKLVRDTMRTARIPGFRPGKAPRQLVLQFYGKERLRAQALENLIDSSLKEAI
EQESIASLGNLQLRDSFEELLGRYQPGEPLSFKAAVDVQPEVQLGTYTGLTVRYSEVPYEAKQVDDQLEQYREQRAVLVP
VEGRAAEVGDTAVIDFAGTKAADGNEIVGGKATDFEVELLPGRLIAGFTEGIIGMQIGESTELALRFPDDYPQQELAGVD
AKFAVSLKDLKIKELPVLDDDFAGDISEFETLEALRAFLEQQQQEQAAEKTRANRDAAIIKALVAETTVDLPETLVNREV
QFLAEQSFRNLQQQGIDPSRIFTEENMPRVRETLRVDAENRLKRTLALAQVARAENIVVEEEQVAARIVELRSELEEEVS
EQALAEFAREEMLTEKILEWLAEHSTIELTLPGEAIEPGSGEDAPPEVAAGATEPEAQPNS

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=369, Percent_Identity=30.8943089430894, Blast_Score=145, Evalue=8e-36,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_GLOVI (Q7NDP0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927141.1
- ProteinModelPortal:   Q7NDP0
- GeneID:   2602598
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr4195
- NMPDR:   fig|251221.1.peg.4195
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   GVIO251221:GLR4195-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 51220; Mature: 51220

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTQEKLPRSQMGLNVEVEGEKSKQAYEKLVRDTMRTARIPGFRPGKAPRQLVLQFYGK
CCCCHHHCCHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ERLRAQALENLIDSSLKEAIEQESIASLGNLQLRDSFEELLGRYQPGEPLSFKAAVDVQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCC
EVQLGTYTGLTVRYSEVPYEAKQVDDQLEQYREQRAVLVPVEGRAAEVGDTAVIDFAGTK
CEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCC
AADGNEIVGGKATDFEVELLPGRLIAGFTEGIIGMQIGESTELALRFPDDYPQQELAGVD
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHEEEECCCCEEEEECCCCCCHHHHHCCC
AKFAVSLKDLKIKELPVLDDDFAGDISEFETLEALRAFLEQQQQEQAAEKTRANRDAAII
CCEEEEHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KALVAETTVDLPETLVNREVQFLAEQSFRNLQQQGIDPSRIFTEENMPRVRETLRVDAEN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCHHH
RLKRTLALAQVARAENIVVEEEQVAARIVELRSELEEEVSEQALAEFAREEMLTEKILEW
HHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEHSTIELTLPGEAIEPGSGEDAPPEVAAGATEPEAQPNS
HHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVTQEKLPRSQMGLNVEVEGEKSKQAYEKLVRDTMRTARIPGFRPGKAPRQLVLQFYGK
CCCCHHHCCHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ERLRAQALENLIDSSLKEAIEQESIASLGNLQLRDSFEELLGRYQPGEPLSFKAAVDVQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCC
EVQLGTYTGLTVRYSEVPYEAKQVDDQLEQYREQRAVLVPVEGRAAEVGDTAVIDFAGTK
CEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCC
AADGNEIVGGKATDFEVELLPGRLIAGFTEGIIGMQIGESTELALRFPDDYPQQELAGVD
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHCHHEEEECCCCEEEEECCCCCCHHHHHCCC
AKFAVSLKDLKIKELPVLDDDFAGDISEFETLEALRAFLEQQQQEQAAEKTRANRDAAII
CCEEEEHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KALVAETTVDLPETLVNREVQFLAEQSFRNLQQQGIDPSRIFTEENMPRVRETLRVDAEN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCHHH
RLKRTLALAQVARAENIVVEEEQVAARIVELRSELEEEVSEQALAEFAREEMLTEKILEW
HHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEHSTIELTLPGEAIEPGSGEDAPPEVAAGATEPEAQPNS
HHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292