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The map label for this gene is grpE

Identifier: 37523763

GI number: 37523763

Start: 4389896

End: 4390486

Strand: Reverse

Name: grpE

Synonym: gvip551

Alternate gene names: 37523763

Gene position: 4390486-4389896 (Counterclockwise)

Preceding gene: 37523769

Following gene: 37523762

Centisome position: 94.24

GC content: 61.08

Gene sequence:

>591_bases
ATGGCAGAAAACGAGCGCACCACCGAGAACTTCCAACCGCAGGATCCTTCCTATGCAGAGGCCGCAACCACCGAGGCGTC
CGCTGCGGAAGCGACGGGATTCATCGCCCGCATCGACCAGCTCGCCGCCGAGAACTCCGACCTGCAAAAAAAACTCGCCG
ACTACGAGCAAAAGTACACGCGGCTGATGGCCGACTTCGACAATTTCCGCAAGCGCACCCAGCGCGAAAAAGACGAACTG
GCCTATTTTGTTTCCGCCAAGCTCCTCAAGGACATCCTGCCGGTCTTCGACAACTTCGATCGCGCCCGCGCCTTCGCCCA
GCCCGACAACGAGCGCGAAGAGAAGCTGCACAACAGCTACCAGCAGGTTTACCGGCAGTTTCTCTCGGTACTCGAAAAGA
TGGGGGTGACGGCCATGGAGGCGATCGGTCAGCCGTTCGACCCGGCCCAGCACGAGGCCATCCTGCGCGAAGAATCGGCC
GGGGTGAGTCAGGAGACGGTCGTGGCGGAGTTGCAAAAGGGCTATTTGCTCGCCGACAAGGTGCTGCGCCCGGCCATGGT
CAAAGTGGCGATCCCCGAAGCGGGTTCCTAG

Upstream 100 bases:

>100_bases
CAAAGGCCGGAGTCTTCATTTTTCCTGGCGGCGGCAGCCCGAAGGTTGGGTCCGCGAGCGTTTAAAATCAAGGTTGCAAA
CGGCACTATATGGCAGAGCG

Downstream 100 bases:

>100_bases
CTGCGGGCAGTTAAGAATTTCGGTGGCCTTCCCGCGCCTTGCAGCGCAGGGTGATAACCTGTTGAGGTCCAAAAGCGGGA
AAGTCCGGTGCGATCCCGGT

Product: heat shock protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MAENERTTENFQPQDPSYAEAATTEASAAEATGFIARIDQLAAENSDLQKKLADYEQKYTRLMADFDNFRKRTQREKDEL
AYFVSAKLLKDILPVFDNFDRARAFAQPDNEREEKLHNSYQQVYRQFLSVLEKMGVTAMEAIGQPFDPAQHEAILREESA
GVSQETVVAELQKGYLLADKVLRPAMVKVAIPEAGS

Sequences:

>Translated_196_residues
MAENERTTENFQPQDPSYAEAATTEASAAEATGFIARIDQLAAENSDLQKKLADYEQKYTRLMADFDNFRKRTQREKDEL
AYFVSAKLLKDILPVFDNFDRARAFAQPDNEREEKLHNSYQQVYRQFLSVLEKMGVTAMEAIGQPFDPAQHEAILREESA
GVSQETVVAELQKGYLLADKVLRPAMVKVAIPEAGS
>Mature_195_residues
AENERTTENFQPQDPSYAEAATTEASAAEATGFIARIDQLAAENSDLQKKLADYEQKYTRLMADFDNFRKRTQREKDELA
YFVSAKLLKDILPVFDNFDRARAFAQPDNEREEKLHNSYQQVYRQFLSVLEKMGVTAMEAIGQPFDPAQHEAILREESAG
VSQETVVAELQKGYLLADKVLRPAMVKVAIPEAGS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=155, Percent_Identity=29.6774193548387, Blast_Score=70, Evalue=8e-13,
Organism=Homo sapiens, GI40255109, Length=152, Percent_Identity=30.9210526315789, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788967, Length=171, Percent_Identity=30.9941520467836, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653432, Length=170, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_GLOVI (Q7NDP1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_927140.1
- ProteinModelPortal:   Q7NDP1
- SMR:   Q7NDP1
- GeneID:   2602597
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.4194
- HOGENOM:   HBG732630
- OMA:   HEAIAYV
- BioCyc:   GVIO251221:GLL4194-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22158; Mature: 22027

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENERTTENFQPQDPSYAEAATTEASAAEATGFIARIDQLAAENSDLQKKLADYEQKYT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLMADFDNFRKRTQREKDELAYFVSAKLLKDILPVFDNFDRARAFAQPDNEREEKLHNSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHH
QQVYRQFLSVLEKMGVTAMEAIGQPFDPAQHEAILREESAGVSQETVVAELQKGYLLADK
HHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHH
VLRPAMVKVAIPEAGS
HHHHHHHHEECCCCCC
>Mature Secondary Structure 
AENERTTENFQPQDPSYAEAATTEASAAEATGFIARIDQLAAENSDLQKKLADYEQKYT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLMADFDNFRKRTQREKDELAYFVSAKLLKDILPVFDNFDRARAFAQPDNEREEKLHNSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHH
QQVYRQFLSVLEKMGVTAMEAIGQPFDPAQHEAILREESAGVSQETVVAELQKGYLLADK
HHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHH
VLRPAMVKVAIPEAGS
HHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292