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The map label for this gene is nccB [H]

Identifier: 37523740

GI number: 37523740

Start: 4371203

End: 4372642

Strand: Reverse

Name: nccB [H]

Synonym: gll4171

Alternate gene names: 37523740

Gene position: 4372642-4371203 (Counterclockwise)

Preceding gene: 37523744

Following gene: 37523739

Centisome position: 93.85

GC content: 70.21

Gene sequence:

>1440_bases
GTGCGCAGCAAAGCGGCAAGGATTTTGATGGTGGCGGTGCTGATGGGAGCGGCGGTGAGCGCTCCCGTGGAAGCCGGCGA
CGAGCGGGGTCACGGGGAGACGACTTTCGCTCCGCAGGAAGTGGCCGGTGGCGGCGAGGTGCGCATCGAGCCGCAGATGC
AGCGGGCATTGGGTTTAAAGGTGGCTGCCGTGGGCGAGCGACCGGTGCGGGCGGGGCTGGCCGTCAACGGTCAAATCGAG
GCGATCCCCGGCCGTTCTTCTGAGATCAACGCCCCGGTGGCGGGCCGGGTCGTGCGCCTGCAGGTGCAGCGCGGCCGGGC
GGTGCGCGCGGGCCAGCCCCTGGCGGTGCTCGATAGTCCCGAGGTGCGGACCCTGGCGCTTGAGGCGGAGCGCGAGCGCG
CCCAGGCCCGCTCCGAGGTGGACCGGCTCAGCGCCGGGGTGCAGCTGGCCCACCAGAACTACGAGCGGGAAAAAGAATTG
GTGGCACTGAAGATCTCCGCCCGCCGCGAATTTCAGCAGGCCGAGGCCGCTTTGCGCGCTTCGGAGGCGGATTTGCGGGC
GGCCCGTTCCCGGCTCGCCCTCAGCGGGGCGGCCTTGAGCACCCGGCTTGCCCAACTGGGGCAAAGCGGCGTGCGTGCGC
GCTCGGACGGCACGGTGGTGCTCTTTGCACCGATTGTCGGGGTGGTGGCAGATCAGCAGGTGACGGCGGGGGAAGCCGTC
GAACCCGGCAAGGCGCTCTTTCGGATTCTCGATGCGACGCAAGTCTGGGCAACCGCCCAGGTCTACGAAAAAGATCTGGG
GCTGGTGCGGGCGGGTCGGTCCATCGAGGTGACGACCCAGAGCTATCCGGGCAGGACGTTTCGCGGCCGCATCGAGAGCA
TCGACCCGGCGGTGGACCCCCAAACGCGCACGCTCGCGGTGCGGGCGGTGCTCACCAACCCGGAGGGGCTGTTGAAGCCG
CAGATGTACGCCCGGTTGCTCCTGGTAAGCGACGGGGCGGCCCGGCCCGTATCGGTCGTGCCCCGCTCGGCGGTGCTGAC
GGCCGACGGTCTTTCGGTGGTCTACCGCAAAGCGGGAACGGACCGCTTTCGGCCGGTGCCGGTCGAATTGGGCCGCAGCT
TCGAAGAGGCGGTGGAGGTGCGCGCAGGCGTGCGCGCGGGCGACGAAGTGGTCGTCGAGCGCGTTTGGCAGTTGCGCGCC
CAGAGTTTGAAGCAGCAGGGGACGGGTGTCGGCGGCGACGAGCACAGCCAAAAGAAAGCATCGAAGCCATCCACGGCCCG
GACCGCGGCGCCCGGGCCGGACGGCGAAATCCCCGCGTGGATCTGGCTTGCGGCAGCGGTGGCACTGACGGTCCTGGGTT
TTCTGGCCGGTCTGCAGGTGGCGGGCCGCCGGGGCGGGCAAAGCGCGGGCGTCTCGACCGGCGCACTCCCCAAGCCCTAG

Upstream 100 bases:

>100_bases
CACAATGCCCATTTCCATTAATTTGAACAGGGTTCAAAATTAGAGGAGTGGACGCGCCGCCGGTTGTCGCGGTCTTAGCG
TCCCCAAGGAGAGACGCCAG

Downstream 100 bases:

>100_bases
GAGGCGGCGATGCTCAATCGGTTGATTTTGCTGTCGCTCGGCGCCCGCTGGCTGGTGGTGCTGGGGGCGGTGGCGGTGCT
GGGCCTGGGCATTGCCACCG

Product: cation efflux system protein CzcB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRSKAARILMVAVLMGAAVSAPVEAGDERGHGETTFAPQEVAGGGEVRIEPQMQRALGLKVAAVGERPVRAGLAVNGQIE
AIPGRSSEINAPVAGRVVRLQVQRGRAVRAGQPLAVLDSPEVRTLALEAERERAQARSEVDRLSAGVQLAHQNYEREKEL
VALKISARREFQQAEAALRASEADLRAARSRLALSGAALSTRLAQLGQSGVRARSDGTVVLFAPIVGVVADQQVTAGEAV
EPGKALFRILDATQVWATAQVYEKDLGLVRAGRSIEVTTQSYPGRTFRGRIESIDPAVDPQTRTLAVRAVLTNPEGLLKP
QMYARLLLVSDGAARPVSVVPRSAVLTADGLSVVYRKAGTDRFRPVPVELGRSFEEAVEVRAGVRAGDEVVVERVWQLRA
QSLKQQGTGVGGDEHSQKKASKPSTARTAAPGPDGEIPAWIWLAAAVALTVLGFLAGLQVAGRRGGQSAGVSTGALPKP

Sequences:

>Translated_479_residues
MRSKAARILMVAVLMGAAVSAPVEAGDERGHGETTFAPQEVAGGGEVRIEPQMQRALGLKVAAVGERPVRAGLAVNGQIE
AIPGRSSEINAPVAGRVVRLQVQRGRAVRAGQPLAVLDSPEVRTLALEAERERAQARSEVDRLSAGVQLAHQNYEREKEL
VALKISARREFQQAEAALRASEADLRAARSRLALSGAALSTRLAQLGQSGVRARSDGTVVLFAPIVGVVADQQVTAGEAV
EPGKALFRILDATQVWATAQVYEKDLGLVRAGRSIEVTTQSYPGRTFRGRIESIDPAVDPQTRTLAVRAVLTNPEGLLKP
QMYARLLLVSDGAARPVSVVPRSAVLTADGLSVVYRKAGTDRFRPVPVELGRSFEEAVEVRAGVRAGDEVVVERVWQLRA
QSLKQQGTGVGGDEHSQKKASKPSTARTAAPGPDGEIPAWIWLAAAVALTVLGFLAGLQVAGRRGGQSAGVSTGALPKP
>Mature_479_residues
MRSKAARILMVAVLMGAAVSAPVEAGDERGHGETTFAPQEVAGGGEVRIEPQMQRALGLKVAAVGERPVRAGLAVNGQIE
AIPGRSSEINAPVAGRVVRLQVQRGRAVRAGQPLAVLDSPEVRTLALEAERERAQARSEVDRLSAGVQLAHQNYEREKEL
VALKISARREFQQAEAALRASEADLRAARSRLALSGAALSTRLAQLGQSGVRARSDGTVVLFAPIVGVVADQQVTAGEAV
EPGKALFRILDATQVWATAQVYEKDLGLVRAGRSIEVTTQSYPGRTFRGRIESIDPAVDPQTRTLAVRAVLTNPEGLLKP
QMYARLLLVSDGAARPVSVVPRSAVLTADGLSVVYRKAGTDRFRPVPVELGRSFEEAVEVRAGVRAGDEVVVERVWQLRA
QSLKQQGTGVGGDEHSQKKASKPSTARTAAPGPDGEIPAWIWLAAAVALTVLGFLAGLQVAGRRGGQSAGVSTGALPKP

Specific function: Component of the NCC cation efflux system that confers resistance to nickel, cobalt and cadmium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=326, Percent_Identity=24.5398773006135, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786787, Length=357, Percent_Identity=24.6498599439776, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082036, Length=310, Percent_Identity=24.5161290322581, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 50578; Mature: 50578

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKAARILMVAVLMGAAVSAPVEAGDERGHGETTFAPQEVAGGGEVRIEPQMQRALGLK
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEECHHHHHHHCCE
VAAVGERPVRAGLAVNGQIEAIPGRSSEINAPVAGRVVRLQVQRGRAVRAGQPLAVLDSP
EEECCCCCCCCCEEECCEEEECCCCCCCCCCCCCCEEEEEEEECCCEECCCCCEEEECCC
EVRTLALEAERERAQARSEVDRLSAGVQLAHQNYEREKELVALKISARREFQQAEAALRA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHH
SEADLRAARSRLALSGAALSTRLAQLGQSGVRARSDGTVVLFAPIVGVVADQQVTAGEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCC
EPGKALFRILDATQVWATAQVYEKDLGLVRAGRSIEVTTQSYPGRTFRGRIESIDPAVDP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCCCC
QTRTLAVRAVLTNPEGLLKPQMYARLLLVSDGAARPVSVVPRSAVLTADGLSVVYRKAGT
CCCEEEEEEEEECCCCCCCHHHHHEEEEEECCCCCCCEECCCCEEEECCCHHHHHHHCCC
DRFRPVPVELGRSFEEAVEVRAGVRAGDEVVVERVWQLRAQSLKQQGTGVGGDEHSQKKA
CCCCCCCHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
SKPSTARTAAPGPDGEIPAWIWLAAAVALTVLGFLAGLQVAGRRGGQSAGVSTGALPKP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRSKAARILMVAVLMGAAVSAPVEAGDERGHGETTFAPQEVAGGGEVRIEPQMQRALGLK
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEECHHHHHHHCCE
VAAVGERPVRAGLAVNGQIEAIPGRSSEINAPVAGRVVRLQVQRGRAVRAGQPLAVLDSP
EEECCCCCCCCCEEECCEEEECCCCCCCCCCCCCCEEEEEEEECCCEECCCCCEEEECCC
EVRTLALEAERERAQARSEVDRLSAGVQLAHQNYEREKELVALKISARREFQQAEAALRA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHH
SEADLRAARSRLALSGAALSTRLAQLGQSGVRARSDGTVVLFAPIVGVVADQQVTAGEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCC
EPGKALFRILDATQVWATAQVYEKDLGLVRAGRSIEVTTQSYPGRTFRGRIESIDPAVDP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCCCC
QTRTLAVRAVLTNPEGLLKPQMYARLLLVSDGAARPVSVVPRSAVLTADGLSVVYRKAGT
CCCEEEEEEEEECCCCCCCHHHHHEEEEEECCCCCCCEECCCCEEEECCCHHHHHHHCCC
DRFRPVPVELGRSFEEAVEVRAGVRAGDEVVVERVWQLRAQSLKQQGTGVGGDEHSQKKA
CCCCCCCHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
SKPSTARTAAPGPDGEIPAWIWLAAAVALTVLGFLAGLQVAGRRGGQSAGVSTGALPKP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961470 [H]