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The map label for this gene is dfrA [H]

Identifier: 37523725

GI number: 37523725

Start: 4355392

End: 4356408

Strand: Reverse

Name: dfrA [H]

Synonym: gll4156

Alternate gene names: 37523725

Gene position: 4356408-4355392 (Counterclockwise)

Preceding gene: 37523728

Following gene: 37523723

Centisome position: 93.5

GC content: 66.96

Gene sequence:

>1017_bases
GTGCGGGCATTCGTGACGGGGGGAACCGGACTGTTGGGCAGCAACCTGGTGCGCCTGCTGGTGGAGCGGGGGCATGCGGT
GCGGGTGTTGGCCCGCGATCCCGAGCGGGCGCGGCGGGTGCTGGGCGAGCTGCCGGTAGAAGTGGTCGCGGGCGATCTGG
CCGAGGTGGACGGGTTTGCCGGTCACCTGGCCGGTTGTGATGTTCTCTTTCACGCGGCGGCCTACTTTCGGGAGTACTTT
CAGCCGGGCGATCACTGGCGGCAGTTGGAACACCTCAACGTGCGCTCGACGGTCGCGTTGCTCAAGGCGGCCGAGCGCTG
GGGCGTTCAAAAAGCGATTTACGTCAGTTCCTCAGGGGTGATCGGGCCAAGGCCGGACGGGCAGCCCGCCGACGAGTCGG
ACCTGCCGGGAGCGCTTGCCATCGACAATCTGTACTTTCGCAGCAAAGTGCTCGCCGAGGCGGAGGTGTACCGGTTTTTA
GCCCATCACGACCTTTCGGTGACGCTCATCTTGCCGGGTTGGATGTTCGGTCCCGGCGACAGCGCCCCCACCGAGAGCGG
CCAGTTGGTGCTCGACTTTCTAGAGGGCAAACTGCCGGGGGTGCTGGAGATTCCCGGTTCGGTGAGCATCGCCGACGCGC
GCGACGTGGCCCTCGCCATGCTGCAGGCCGTGGAGCACGGTCGCAGTGGCGGGCGCTACATCGTCTCGGGCGAGAGTTAC
GACTTTGTGCAATTGATGGAGAGCCTCGCCCGGGTGAGCGGGAAGACCACCCCGGCCCTGCGCATTCCCTACAGCGCCGC
TCTGGCGATCGCCTGGGTCTCGCAGAACTTTGCAAAGCTCAGTGGCGGCAAGACGGTGCTCACCACCAGCGGCCTGCGCA
CGCTGGCCGATTCGGTGCGCCTCGACAGCGGCAAAGCCCAGCGGGAACTGGGCTTTGTGGCCCGGCCGCTGGAGGAGACC
CTGCGCGACGAGGTCGCTTGGTTCCGCACCCGGGGCAAAGTTGCGGCCGGCCGCTAG

Upstream 100 bases:

>100_bases
GTCCTTTCCCATGGTCAGCTAGAGGCGGCCGGTTGACCAACCGCTTGCCTTTTTGTACTTTAGATATATATCTAGAAGAT
ATAAGCAAAGGAGTGCAATT

Downstream 100 bases:

>100_bases
ACGAAGCTGCGGTCGACATAGCACCAGCGCCAGTCTTCGCCGGGCTCGAAGGAGCGCACGATCGGGTGGCCGCTTTGCTG
ATAATGTCGCAATCGATGTA

Product: dihydroflavonol-4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRAFVTGGTGLLGSNLVRLLVERGHAVRVLARDPERARRVLGELPVEVVAGDLAEVDGFAGHLAGCDVLFHAAAYFREYF
QPGDHWRQLEHLNVRSTVALLKAAERWGVQKAIYVSSSGVIGPRPDGQPADESDLPGALAIDNLYFRSKVLAEAEVYRFL
AHHDLSVTLILPGWMFGPGDSAPTESGQLVLDFLEGKLPGVLEIPGSVSIADARDVALAMLQAVEHGRSGGRYIVSGESY
DFVQLMESLARVSGKTTPALRIPYSAALAIAWVSQNFAKLSGGKTVLTTSGLRTLADSVRLDSGKAQRELGFVARPLEET
LRDEVAWFRTRGKVAAGR

Sequences:

>Translated_338_residues
MRAFVTGGTGLLGSNLVRLLVERGHAVRVLARDPERARRVLGELPVEVVAGDLAEVDGFAGHLAGCDVLFHAAAYFREYF
QPGDHWRQLEHLNVRSTVALLKAAERWGVQKAIYVSSSGVIGPRPDGQPADESDLPGALAIDNLYFRSKVLAEAEVYRFL
AHHDLSVTLILPGWMFGPGDSAPTESGQLVLDFLEGKLPGVLEIPGSVSIADARDVALAMLQAVEHGRSGGRYIVSGESY
DFVQLMESLARVSGKTTPALRIPYSAALAIAWVSQNFAKLSGGKTVLTTSGLRTLADSVRLDSGKAQRELGFVARPLEET
LRDEVAWFRTRGKVAAGR
>Mature_338_residues
MRAFVTGGTGLLGSNLVRLLVERGHAVRVLARDPERARRVLGELPVEVVAGDLAEVDGFAGHLAGCDVLFHAAAYFREYF
QPGDHWRQLEHLNVRSTVALLKAAERWGVQKAIYVSSSGVIGPRPDGQPADESDLPGALAIDNLYFRSKVLAEAEVYRFL
AHHDLSVTLILPGWMFGPGDSAPTESGQLVLDFLEGKLPGVLEIPGSVSIADARDVALAMLQAVEHGRSGGRYIVSGESY
DFVQLMESLARVSGKTTPALRIPYSAALAIAWVSQNFAKLSGGKTVLTTSGLRTLADSVRLDSGKAQRELGFVARPLEET
LRDEVAWFRTRGKVAAGR

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081792, Length=349, Percent_Identity=27.5071633237822, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71987463, Length=343, Percent_Identity=27.6967930029155, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17570557, Length=358, Percent_Identity=27.0949720670391, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36469; Mature: 36469

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFVTGGTGLLGSNLVRLLVERGHAVRVLARDPERARRVLGELPVEVVAGDLAEVDGFA
CCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCHH
GHLAGCDVLFHAAAYFREYFQPGDHWRQLEHLNVRSTVALLKAAERWGVQKAIYVSSSGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCC
IGPRPDGQPADESDLPGALAIDNLYFRSKVLAEAEVYRFLAHHDLSVTLILPGWMFGPGD
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
SAPTESGQLVLDFLEGKLPGVLEIPGSVSIADARDVALAMLQAVEHGRSGGRYIVSGESY
CCCCCCCCEEEEECCCCCCCEEECCCCEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCC
DFVQLMESLARVSGKTTPALRIPYSAALAIAWVSQNFAKLSGGKTVLTTSGLRTLADSVR
CHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHC
LDSGKAQRELGFVARPLEETLRDEVAWFRTRGKVAAGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRAFVTGGTGLLGSNLVRLLVERGHAVRVLARDPERARRVLGELPVEVVAGDLAEVDGFA
CCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCHH
GHLAGCDVLFHAAAYFREYFQPGDHWRQLEHLNVRSTVALLKAAERWGVQKAIYVSSSGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCC
IGPRPDGQPADESDLPGALAIDNLYFRSKVLAEAEVYRFLAHHDLSVTLILPGWMFGPGD
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
SAPTESGQLVLDFLEGKLPGVLEIPGSVSIADARDVALAMLQAVEHGRSGGRYIVSGESY
CCCCCCCCEEEEECCCCCCCEEECCCCEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCC
DFVQLMESLARVSGKTTPALRIPYSAALAIAWVSQNFAKLSGGKTVLTTSGLRTLADSVR
CHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHC
LDSGKAQRELGFVARPLEETLRDEVAWFRTRGKVAAGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]