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The map label for this gene is dnaG [H]

Identifier: 37523652

GI number: 37523652

Start: 4280864

End: 4282780

Strand: Reverse

Name: dnaG [H]

Synonym: gvip547

Alternate gene names: 37523652

Gene position: 4282780-4280864 (Counterclockwise)

Preceding gene: 37523655

Following gene: 37523650

Centisome position: 91.92

GC content: 62.23

Gene sequence:

>1917_bases
GTGAGCGCCCCGCAGTTACACCCACGCACCATCGACGAGGTCCGCGCCAAGGCGGATCTGGTCGATGTCGTGTCCGAGCG
GGTAGTCCTGCGCAAGGCGGGGCGCGACTTCAAGGGACTGTGCCCCTTTCACGAGGACCGCAGCCCCAGTTTTTATGTCT
CGCCGGGCAAGCAGATCTATAAGTGCTTTGCCTGCGGCGCCTCGGGCGACGTGTTCAAGTTCGTTATGGAACTGGACAAG
TCCGCCTTTGGCGAGGTGGTGCTGGAACTGGCCCGCCGCTTCGGCGTGCCGGTCCAGACCCTCAAGCCCGAGCAAAAAGC
CGAGTACACCCGCAAGCTCTCCAAACAGCAGCAACTGGGCGAAATTCTCGAACTGGCGGCCCAGTTTTATAGTTACGCCC
TCTGGGGCGAGCGCGGAGCGGCGGCGAGGCAGTATCTGCTGGAGGCGCGTGCGCTCAGCGAAAAGACGATTCGCCAGTTT
CGCCTGGGCTTTGCCCCCGAGGGCTGGCAGTCGCTTTATACCTACTTGGTCGACCAGAAGCGCTTTCCAGCTTCGCTGGT
GGAAGAGGCGGGGCTGATCATTCCTAGAAGCAGCGGCCAGGGTTATTACGACCGCTTTCGTCACCGACTGATCATTCCTA
TATGCGATCTCAAAGGCAAGGTGATCGCCTTCGGGGGGCGGGCGATGGGCGACGAGCAGCCCAAGTACCTCAATTCGCCC
GAGACGGAACTCTTTAACAAGGGTCAGACGCTCTACGCCCTCGATCAGGCGCGCGAGTCGGTGGGCCGCACCGACGGGGC
GATCGTCGTCGAAGGGTACTTCGATGCGATTGCCCTGCACCAGTCGGGGATCAGCCACGTGGTGGCCACGCTCGGCACCT
CGCTGCGGACCGACCAGATCAAGCAGCTGTTGCGCTACACCGAATCGAAGCGCGTCGTGCTCAATTTCGACGCCGATGCG
GCGGGGGTGCAGGCTGCCGAGCGTGCGATTGCCGAGTTGCGGCCCCTGGTGGTCAAATCCGGTGTGCAGCTGCGGATCGT
GGCGCTGCCGGCGGGCAAGGACCCGGACGAATTTTTGCGTTCCCACCCGCCCCAGGAGTATCTGAAGCTTGCCAGTGAAG
CGCCTTTGTGGATCGACTGGCAAATCGAGCGGCTGTTTGCCGGGCGGGATCTGAGCTTGGCCGCTGATTTTCAGCAGGTG
AGCCAGGGATTGGTGGAACTGCTGTCGGGTCTGCTCAGTTCGATGACCCGGGCGCACTATATCCACCTCATCGCCGGACA
GCTTTCCCAGGGCAACGGCCGGCTCGCTTCCCAACTCGAAGACGAACTGCGGCGGCGCATCCGCACCCATCGCTGGGGCG
GTGGGGACGGCAAGTCCAAAAAAAAGCGCGAACCGTTTCCGCCCGCCTGTTTTCAGGCCGAGGTGCAGCTCATCCAGATC
TATTTGCACTTTGCGGAGTACCGCGAGGACATTCACCGCGGCCTCGACGAGCACGATCTCGAATTTTCGGTGCTGCACCA
CCGCCAGCTGTGGCAGAAGATACTGCAGTTGCGCGAGGAAATTGGCGAGGACGACGACGTGGTGGTGGCCTTGCGCACGA
TGTACGCGGGCGATCCTGAACTCAATCAGCGCCTCGGGCAACTGCTCTGGCTCAACGAACACAACCGCATCGCCCTGATG
CGGCCGCGCATGGTGATTCGGGCGGGCCTTGCCACGCTGCAGCTCGATCGCTGCGAGCGGCGCTACAACTACCTCAACCA
GCTGTGCGACGAAGCGGAGCGGCGCGGCGCCTGGGAGGAAGCCGACTACTTCGTCGAGCAACGCAACGCCGAATACCACC
GCATCAACGACCTCAAGACCCACCTTACGCTCAAGCTTGGTGAGATATCTGAAACCGCTGTCTGGCAGGAGTCCTGA

Upstream 100 bases:

>100_bases
TGCGAAACCGGCCGGATCGCAGCCCCAGATATGGTGTGGTCTGAGCCTTTTGATCGGAGTTTTCACGCTACAATAATTTC
CCTTCACCGGCACGATCCCC

Downstream 100 bases:

>100_bases
GTTGGAGCGATTTGGGGTGGCCCAGATGTCGGCAGGCCGATGAGAAATCTTGATGGTCAACGATTTTTAACCCGACGCCT
GATATTTGGCGGTTGGTTTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MSAPQLHPRTIDEVRAKADLVDVVSERVVLRKAGRDFKGLCPFHEDRSPSFYVSPGKQIYKCFACGASGDVFKFVMELDK
SAFGEVVLELARRFGVPVQTLKPEQKAEYTRKLSKQQQLGEILELAAQFYSYALWGERGAAARQYLLEARALSEKTIRQF
RLGFAPEGWQSLYTYLVDQKRFPASLVEEAGLIIPRSSGQGYYDRFRHRLIIPICDLKGKVIAFGGRAMGDEQPKYLNSP
ETELFNKGQTLYALDQARESVGRTDGAIVVEGYFDAIALHQSGISHVVATLGTSLRTDQIKQLLRYTESKRVVLNFDADA
AGVQAAERAIAELRPLVVKSGVQLRIVALPAGKDPDEFLRSHPPQEYLKLASEAPLWIDWQIERLFAGRDLSLAADFQQV
SQGLVELLSGLLSSMTRAHYIHLIAGQLSQGNGRLASQLEDELRRRIRTHRWGGGDGKSKKKREPFPPACFQAEVQLIQI
YLHFAEYREDIHRGLDEHDLEFSVLHHRQLWQKILQLREEIGEDDDVVVALRTMYAGDPELNQRLGQLLWLNEHNRIALM
RPRMVIRAGLATLQLDRCERRYNYLNQLCDEAERRGAWEEADYFVEQRNAEYHRINDLKTHLTLKLGEISETAVWQES

Sequences:

>Translated_638_residues
MSAPQLHPRTIDEVRAKADLVDVVSERVVLRKAGRDFKGLCPFHEDRSPSFYVSPGKQIYKCFACGASGDVFKFVMELDK
SAFGEVVLELARRFGVPVQTLKPEQKAEYTRKLSKQQQLGEILELAAQFYSYALWGERGAAARQYLLEARALSEKTIRQF
RLGFAPEGWQSLYTYLVDQKRFPASLVEEAGLIIPRSSGQGYYDRFRHRLIIPICDLKGKVIAFGGRAMGDEQPKYLNSP
ETELFNKGQTLYALDQARESVGRTDGAIVVEGYFDAIALHQSGISHVVATLGTSLRTDQIKQLLRYTESKRVVLNFDADA
AGVQAAERAIAELRPLVVKSGVQLRIVALPAGKDPDEFLRSHPPQEYLKLASEAPLWIDWQIERLFAGRDLSLAADFQQV
SQGLVELLSGLLSSMTRAHYIHLIAGQLSQGNGRLASQLEDELRRRIRTHRWGGGDGKSKKKREPFPPACFQAEVQLIQI
YLHFAEYREDIHRGLDEHDLEFSVLHHRQLWQKILQLREEIGEDDDVVVALRTMYAGDPELNQRLGQLLWLNEHNRIALM
RPRMVIRAGLATLQLDRCERRYNYLNQLCDEAERRGAWEEADYFVEQRNAEYHRINDLKTHLTLKLGEISETAVWQES
>Mature_637_residues
SAPQLHPRTIDEVRAKADLVDVVSERVVLRKAGRDFKGLCPFHEDRSPSFYVSPGKQIYKCFACGASGDVFKFVMELDKS
AFGEVVLELARRFGVPVQTLKPEQKAEYTRKLSKQQQLGEILELAAQFYSYALWGERGAAARQYLLEARALSEKTIRQFR
LGFAPEGWQSLYTYLVDQKRFPASLVEEAGLIIPRSSGQGYYDRFRHRLIIPICDLKGKVIAFGGRAMGDEQPKYLNSPE
TELFNKGQTLYALDQARESVGRTDGAIVVEGYFDAIALHQSGISHVVATLGTSLRTDQIKQLLRYTESKRVVLNFDADAA
GVQAAERAIAELRPLVVKSGVQLRIVALPAGKDPDEFLRSHPPQEYLKLASEAPLWIDWQIERLFAGRDLSLAADFQQVS
QGLVELLSGLLSSMTRAHYIHLIAGQLSQGNGRLASQLEDELRRRIRTHRWGGGDGKSKKKREPFPPACFQAEVQLIQIY
LHFAEYREDIHRGLDEHDLEFSVLHHRQLWQKILQLREEIGEDDDVVVALRTMYAGDPELNQRLGQLLWLNEHNRIALMR
PRMVIRAGLATLQLDRCERRYNYLNQLCDEAERRGAWEEADYFVEQRNAEYHRINDLKTHLTLKLGEISETAVWQES

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=354, Percent_Identity=40.9604519774011, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72704; Mature: 72573

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPQLHPRTIDEVRAKADLVDVVSERVVLRKAGRDFKGLCPFHEDRSPSFYVSPGKQIY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHH
KCFACGASGDVFKFVMELDKSAFGEVVLELARRFGVPVQTLKPEQKAEYTRKLSKQQQLG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHH
EILELAAQFYSYALWGERGAAARQYLLEARALSEKTIRQFRLGFAPEGWQSLYTYLVDQK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
RFPASLVEEAGLIIPRSSGQGYYDRFRHRLIIPICDLKGKVIAFGGRAMGDEQPKYLNSP
CCCHHHHHHCCEEEECCCCCCHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
ETELFNKGQTLYALDQARESVGRTDGAIVVEGYFDAIALHQSGISHVVATLGTSLRTDQI
HHHHHCCCCEEEHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KQLLRYTESKRVVLNFDADAAGVQAAERAIAELRPLVVKSGVQLRIVALPAGKDPDEFLR
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
SHPPQEYLKLASEAPLWIDWQIERLFAGRDLSLAADFQQVSQGLVELLSGLLSSMTRAHY
CCCCHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHLIAGQLSQGNGRLASQLEDELRRRIRTHRWGGGDGKSKKKREPFPPACFQAEVQLIQI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YLHFAEYREDIHRGLDEHDLEFSVLHHRQLWQKILQLREEIGEDDDVVVALRTMYAGDPE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCHH
LNQRLGQLLWLNEHNRIALMRPRMVIRAGLATLQLDRCERRYNYLNQLCDEAERRGAWEE
HHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ADYFVEQRNAEYHRINDLKTHLTLKLGEISETAVWQES
HHHHHHHCCCCHHHHHHHHHEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SAPQLHPRTIDEVRAKADLVDVVSERVVLRKAGRDFKGLCPFHEDRSPSFYVSPGKQIY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHH
KCFACGASGDVFKFVMELDKSAFGEVVLELARRFGVPVQTLKPEQKAEYTRKLSKQQQLG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHH
EILELAAQFYSYALWGERGAAARQYLLEARALSEKTIRQFRLGFAPEGWQSLYTYLVDQK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
RFPASLVEEAGLIIPRSSGQGYYDRFRHRLIIPICDLKGKVIAFGGRAMGDEQPKYLNSP
CCCHHHHHHCCEEEECCCCCCHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
ETELFNKGQTLYALDQARESVGRTDGAIVVEGYFDAIALHQSGISHVVATLGTSLRTDQI
HHHHHCCCCEEEHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KQLLRYTESKRVVLNFDADAAGVQAAERAIAELRPLVVKSGVQLRIVALPAGKDPDEFLR
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
SHPPQEYLKLASEAPLWIDWQIERLFAGRDLSLAADFQQVSQGLVELLSGLLSSMTRAHY
CCCCHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHLIAGQLSQGNGRLASQLEDELRRRIRTHRWGGGDGKSKKKREPFPPACFQAEVQLIQI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YLHFAEYREDIHRGLDEHDLEFSVLHHRQLWQKILQLREEIGEDDDVVVALRTMYAGDPE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCHH
LNQRLGQLLWLNEHNRIALMRPRMVIRAGLATLQLDRCERRYNYLNQLCDEAERRGAWEE
HHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ADYFVEQRNAEYHRINDLKTHLTLKLGEISETAVWQES
HHHHHHHCCCCHHHHHHHHHEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA