Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 37523584

GI number: 37523584

Start: 4217674

End: 4218999

Strand: Direct

Name: miaB

Synonym: glr4015

Alternate gene names: 37523584

Gene position: 4217674-4218999 (Clockwise)

Preceding gene: 37523581

Following gene: 37523585

Centisome position: 90.53

GC content: 62.97

Gene sequence:

>1326_bases
ATGGAATCCACCACCAACCCCCAGGGCAAAACCGCCTGCCTCGTCACCTTCGGCTGCCAGATGAACAAGGCCGACTCCGA
GCGCATGGCCGGGGCACTCACCCACCTGGGCTACCGGATCGTCGATGAAAGCGACACGGCCGACTTGGTCCTGTTCAACA
CCTGCACCATCCGCGACAACGCCGAGCAAAAGGTCTATTCCTACCTGGGTCAGCAGGCCAGGCGCAAACAGCGCGACCCG
CACATCACGCTGGTATTGGCGGGCTGTGTGGCCCAGCAGGAGGGTGAAAAATTGCTCCGGCGCGTGCCGGAACTGGATCT
GGTGATGGGGCCGCAGCACGTCAATCGCCTTTCGGATTTGCTCGAGCGGGTGGCCGAGGGTGAGCAGGTGGTGGCCACCG
AGCCCATCGAAATTCTCGAAGACATCACCAAGCCCCGCCGCGACAGCGCCGTCACCGCCTGGGCCAACATCATCTACGGC
TGCAACGAAGGATGCACCTACTGCATCGTGCCCTCGGTGCGCGGGCGCGAACAATCGCGCACTCCCGAGGCGATCAAAGC
CGAAATTTGTGAACTGGGCGCGTCGGGCTACAAAGAAGTGACGCTTCTCGGCCAAAATATCGACGCCTACGGCCGCGACA
TCGGCACCAACCTGGCTTCACTGCTGCGCTTTATCCACAACGCGCCGGGCATCGAACGCCTGCGCTTCGCCACTTCCCAC
CCGCGCTACTTTAGCGACGAACTGATTGCCACCTGTGCCGAATTGCCGAAAGTTTGCGAGCACTTTCATATTCCTTTTCA
GTCGGGCGACAACGAAGTGCTCCGGCGCATGGCCCGGGGGTATACCCACGAAAAATATCGCGCCATCATCGAAAAGATCC
GCGCCATCCTGCCCGACGCCGCCATCTCGGCGGACCTCATCGTCGGCTTTCCCGGCGAAACGGAGGCGCAGTTTGAAAAT
TCCCTGCGCCTGGTGGACGAACTGGAATTCGACGCCCTCAATACGGCGGCCTATTCGCCCCGCCCGGGCACCCCGGCGGC
GCGCTGGCCCGGGCAATTGGACGACGAAGTCAAGCAGGACCGCCTGCAGCGTACCAACCGGCTGGTGGCCCAAAAAGCTT
TCGAGCGCTCCCAGCGCTATCTGGGCCGCACCGAGCAGGTGCTGGTCGAAGAGACCAACCCCCGCGACCGCTCCCAGGTG
GTGGGCCGCACCCGCACCAACCGCCTGGTCTTTTTTGCGGGCGAACTTGCGAAACTGCGCGGCCAACTGGTCGATGTGCA
GATCACCGCGGCGCGCGCCTTTTCGCTGACGGGCACGGCAAGCTAA

Upstream 100 bases:

>100_bases
TACCTCGCGTAGGCTGATCCTTTCAAGAATAACAGGCTTGGGCAGTCGGCCGATGAAAGTAGTAGCCTGGAGGGTGTCCG
CAACCCGCCCCCATTTCGCC

Downstream 100 bases:

>100_bases
CCGATGGGCGAACGGCTCGGTATCTTCGGCGGCACCTTCAATCCGGTCCACCGGGGACACCTCGCCATGGCCCGCGCCGC
GCGCGACCGCTGCGGGCTCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MESTTNPQGKTACLVTFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDNAEQKVYSYLGQQARRKQRDP
HITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDLLERVAEGEQVVATEPIEILEDITKPRRDSAVTAWANIIYG
CNEGCTYCIVPSVRGREQSRTPEAIKAEICELGASGYKEVTLLGQNIDAYGRDIGTNLASLLRFIHNAPGIERLRFATSH
PRYFSDELIATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDAAISADLIVGFPGETEAQFEN
SLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQDRLQRTNRLVAQKAFERSQRYLGRTEQVLVEETNPRDRSQV
VGRTRTNRLVFFAGELAKLRGQLVDVQITAARAFSLTGTAS

Sequences:

>Translated_441_residues
MESTTNPQGKTACLVTFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDNAEQKVYSYLGQQARRKQRDP
HITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDLLERVAEGEQVVATEPIEILEDITKPRRDSAVTAWANIIYG
CNEGCTYCIVPSVRGREQSRTPEAIKAEICELGASGYKEVTLLGQNIDAYGRDIGTNLASLLRFIHNAPGIERLRFATSH
PRYFSDELIATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDAAISADLIVGFPGETEAQFEN
SLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQDRLQRTNRLVAQKAFERSQRYLGRTEQVLVEETNPRDRSQV
VGRTRTNRLVFFAGELAKLRGQLVDVQITAARAFSLTGTAS
>Mature_441_residues
MESTTNPQGKTACLVTFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDNAEQKVYSYLGQQARRKQRDP
HITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDLLERVAEGEQVVATEPIEILEDITKPRRDSAVTAWANIIYG
CNEGCTYCIVPSVRGREQSRTPEAIKAEICELGASGYKEVTLLGQNIDAYGRDIGTNLASLLRFIHNAPGIERLRFATSH
PRYFSDELIATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDAAISADLIVGFPGETEAQFEN
SLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQDRLQRTNRLVAQKAFERSQRYLGRTEQVLVEETNPRDRSQV
VGRTRTNRLVFFAGELAKLRGQLVDVQITAARAFSLTGTAS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=470, Percent_Identity=32.9787234042553, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI28872782, Length=470, Percent_Identity=32.9787234042553, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI93277076, Length=447, Percent_Identity=29.3064876957494, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=40.5466970387244, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=29.010989010989, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=34.1991341991342, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=366, Percent_Identity=30.327868852459, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI21356207, Length=436, Percent_Identity=33.7155963302752, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=31.0043668122271, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_GLOVI (Q7NE65)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926961.1
- ProteinModelPortal:   Q7NE65
- SMR:   Q7NE65
- GeneID:   2602418
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr4015
- NMPDR:   fig|251221.1.peg.4015
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14333
- BioCyc:   GVIO251221:GLR4015-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49263; Mature: 49263

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTTNPQGKTACLVTFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDN
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEECCCC
AEQKVYSYLGQQARRKQRDPHITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDL
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LERVAEGEQVVATEPIEILEDITKPRRDSAVTAWANIIYGCNEGCTYCIVPSVRGREQSR
HHHHHCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
TPEAIKAEICELGASGYKEVTLLGQNIDAYGRDIGTNLASLLRFIHNAPGIERLRFATSH
CHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
PRYFSDELIATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
AISADLIVGFPGETEAQFENSLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
RLQRTNRLVAQKAFERSQRYLGRTEQVLVEETNPRDRSQVVGRTRTNRLVFFAGELAKLR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEECHHHHHC
GQLVDVQITAARAFSLTGTAS
CCEEEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MESTTNPQGKTACLVTFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDN
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEECCCC
AEQKVYSYLGQQARRKQRDPHITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDL
HHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LERVAEGEQVVATEPIEILEDITKPRRDSAVTAWANIIYGCNEGCTYCIVPSVRGREQSR
HHHHHCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
TPEAIKAEICELGASGYKEVTLLGQNIDAYGRDIGTNLASLLRFIHNAPGIERLRFATSH
CHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
PRYFSDELIATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
AISADLIVGFPGETEAQFENSLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
RLQRTNRLVAQKAFERSQRYLGRTEQVLVEETNPRDRSQVVGRTRTNRLVFFAGELAKLR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEECHHHHHC
GQLVDVQITAARAFSLTGTAS
CCEEEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292