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The map label for this gene is tuf

Identifier: 37523497

GI number: 37523497

Start: 4122116

End: 4123345

Strand: Direct

Name: tuf

Synonym: gvip535

Alternate gene names: 37523497

Gene position: 4122116-4123345 (Clockwise)

Preceding gene: 37523496

Following gene: 37523498

Centisome position: 88.48

GC content: 63.09

Gene sequence:

>1230_bases
ATGGCTCGCGCTAAGTTCGAACGGAATAAACCGCACGTCAACATCGGGACCATCGGTCACGTCGACCATGGCAAGACGAC
GCTGACGGCGGCCATCACCATGACGCTGGCCGCGCTCGGCCGCGCCAAGGCCAAGAAGTACGACGAAATTGACCAGGCCC
CCGAAGAGAAAGCCCGCGGCATCACGATCAACACCGCCCACGTCGAGTACGAGACCGAGAAGCGCCACTACGCCCACGTG
GATTGCCCCGGTCACGCCGACTACGTCAAGAACATGATCACCGGCGCCGCCCAGATGGATGGTGCCATCCTGGTGGTCTC
GGCGGCGGACGGTCCGATGCCCCAGACCCGCGAGCACATCCTGCTTGCCCGTCAGGTCGGCGTGCCCAATATCGTCGTCT
TCTTGAACAAAAAAGACCAGCTCGACGACCCCGAATTGCTGGAACTGGTCGAACTGGAAGTGCGCGAGCTGCTTTCTAAG
TACGACTTTCCCGGCGATGACGTCCCGATCGTGGCGGGTTCGGCCCTGATGGCCCTCGAGAAGATGGCCTCCGAGCCGAA
GCTCATCCGCGGCAAGGACGATTGGGTCGATTGCATCTACTCGCTGATGGACGCGGTGGACGCCTACATCCCCACTCCCG
AGCGGGCGATCGACAAGCCGTTCTTGATGGCGGTCGAAGACGTCTTCTCGATCACCGGCCGCGGCACTGTCGCCACCGGC
CGCATCGAGCGCGGCAAGGTCAAAGTCGGCGAGACCATCGAGTTGGTGGGCATCCGCGGGACCCGCTCCACCACCGTCAC
CGGTCTGGAGATGTTCCAGAAGTCCCTCGATGAGGGCCTGGCGGGCGACAACATCGGCGTCCTCCTGCGCGGGATCAAAA
AAGAGGACGTCGAGCGCGGCATGGTGCTTGCCAAGCCCGGCTCGATCACCCCCCACACCCAGTTCGAGGGCGAGGTCTAC
ATCCTCAGCAAAGAAGAAGGTGGCCGCCATACCCCCTTCTTCGCCGGCTACCGCCCGCAGTTCTACGTGCGCACCACCGA
CGTGACCGGCACCATCGTCACCTTCACCGACGACGAAGGCAAGTCCGCCGAGATGGTGATGCCTGGCGACCGCATCAAGA
TGACCGTTGAACTGATCAACCCAATCGCCATCGAGGACGGCATGCGCTTTGCCATCCGCGAGGGCGGCCGCACCGTGGGC
GCGGGCGTTGTCTCCAAGATCCTGAAGTAA

Upstream 100 bases:

>100_bases
CTCGATGGAGTTCAGCCATTACGAAGAAGTGCCGCGCAACGTCGCCGAGACGATCATCGCCAAAAACAAAGGGAACGCTT
AACTAGAGGAAAGGAAACCC

Downstream 100 bases:

>100_bases
AGTAGATTCACCCGCAAAGGGTGGGGCGATCTCGCCCCACCCTTTGCTCTGAGGATTCCAAGCCATGGCGCTGACCCAGC
AAAAAATTCGCATTCGTCTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMTLAALGRAKAKKYDEIDQAPEEKARGITINTAHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPNIVVFLNKKDQLDDPELLELVELEVRELLSK
YDFPGDDVPIVAGSALMALEKMASEPKLIRGKDDWVDCIYSLMDAVDAYIPTPERAIDKPFLMAVEDVFSITGRGTVATG
RIERGKVKVGETIELVGIRGTRSTTVTGLEMFQKSLDEGLAGDNIGVLLRGIKKEDVERGMVLAKPGSITPHTQFEGEVY
ILSKEEGGRHTPFFAGYRPQFYVRTTDVTGTIVTFTDDEGKSAEMVMPGDRIKMTVELINPIAIEDGMRFAIREGGRTVG
AGVVSKILK

Sequences:

>Translated_409_residues
MARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMTLAALGRAKAKKYDEIDQAPEEKARGITINTAHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPNIVVFLNKKDQLDDPELLELVELEVRELLSK
YDFPGDDVPIVAGSALMALEKMASEPKLIRGKDDWVDCIYSLMDAVDAYIPTPERAIDKPFLMAVEDVFSITGRGTVATG
RIERGKVKVGETIELVGIRGTRSTTVTGLEMFQKSLDEGLAGDNIGVLLRGIKKEDVERGMVLAKPGSITPHTQFEGEVY
ILSKEEGGRHTPFFAGYRPQFYVRTTDVTGTIVTFTDDEGKSAEMVMPGDRIKMTVELINPIAIEDGMRFAIREGGRTVG
AGVVSKILK
>Mature_408_residues
ARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMTLAALGRAKAKKYDEIDQAPEEKARGITINTAHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPNIVVFLNKKDQLDDPELLELVELEVRELLSKY
DFPGDDVPIVAGSALMALEKMASEPKLIRGKDDWVDCIYSLMDAVDAYIPTPERAIDKPFLMAVEDVFSITGRGTVATGR
IERGKVKVGETIELVGIRGTRSTTVTGLEMFQKSLDEGLAGDNIGVLLRGIKKEDVERGMVLAKPGSITPHTQFEGEVYI
LSKEEGGRHTPFFAGYRPQFYVRTTDVTGTIVTFTDDEGKSAEMVMPGDRIKMTVELINPIAIEDGMRFAIREGGRTVGA
GVVSKILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=407, Percent_Identity=56.019656019656, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=449, Percent_Identity=28.9532293986637, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI5729864, Length=449, Percent_Identity=28.9532293986637, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=30.2222222222222, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=30.0884955752212, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI194018520, Length=447, Percent_Identity=26.1744966442953, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI194018522, Length=447, Percent_Identity=26.1744966442953, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI194097354, Length=447, Percent_Identity=26.1744966442953, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI46094014, Length=444, Percent_Identity=27.2522522522523, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI54607086, Length=329, Percent_Identity=26.7477203647416, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=278, Percent_Identity=27.6978417266187, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=23.2346241457859, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=24.5614035087719, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310132016, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310110807, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310123363, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=72.0588235294118, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=72.0588235294118, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI2367247, Length=391, Percent_Identity=30.1790281329923, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994988, Length=284, Percent_Identity=31.3380281690141, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1789108, Length=337, Percent_Identity=28.486646884273, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=414, Percent_Identity=49.7584541062802, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=400, Percent_Identity=45.25, Blast_Score=340, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.5555555555556, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.5555555555556, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32566629, Length=459, Percent_Identity=28.322440087146, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=29.0909090909091, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=457, Percent_Identity=26.2582056892779, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=453, Percent_Identity=26.2693156732892, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=281, Percent_Identity=29.5373665480427, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.734693877551, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=242, Percent_Identity=26.4462809917355, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=407, Percent_Identity=63.3906633906634, Blast_Score=524, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=458, Percent_Identity=28.3842794759825, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=458, Percent_Identity=28.3842794759825, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=23.3480176211454, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=449, Percent_Identity=25.6124721603563, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=26.6903914590747, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=286, Percent_Identity=24.8251748251748, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=174, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=403, Percent_Identity=57.3200992555831, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=403, Percent_Identity=57.3200992555831, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=401, Percent_Identity=49.3765586034913, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=29.0465631929047, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=29.0465631929047, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=29.0465631929047, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=29.0465631929047, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=29.3986636971047, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=29.3986636971047, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45550900, Length=454, Percent_Identity=29.0748898678414, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI17137380, Length=447, Percent_Identity=27.2930648769575, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=31.629392971246, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24647048, Length=277, Percent_Identity=27.4368231046931, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647046, Length=277, Percent_Identity=27.4368231046931, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=32.6666666666667, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28574573, Length=148, Percent_Identity=33.1081081081081, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_GLOVI (P50064)

Other databases:

- EMBL:   BA000045
- EMBL:   U09433
- RefSeq:   NP_926874.1
- ProteinModelPortal:   P50064
- SMR:   P50064
- GeneID:   2602331
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3928
- HOGENOM:   HBG307581
- OMA:   ERKPHIN
- ProtClustDB:   PRK00049
- BioCyc:   GVIO251221:GLR3928-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44761; Mature: 44630

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMTLAALGRAKAKKYDEIDQAPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ITINTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPNIVVFLNKKDQLDDPELLELVELEVRELLSKYDFPGDDVPIVAGSALMALE
EEEHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
KMASEPKLIRGKDDWVDCIYSLMDAVDAYIPTPERAIDKPFLMAVEDVFSITGRGTVATG
HHCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEC
RIERGKVKVGETIELVGIRGTRSTTVTGLEMFQKSLDEGLAGDNIGVLLRGIKKEDVERG
CEECCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHEECCCHHHHHCC
MVLAKPGSITPHTQFEGEVYILSKEEGGRHTPFFAGYRPQFYVRTTDVTGTIVTFTDDEG
EEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCC
KSAEMVMPGDRIKMTVELINPIAIEDGMRFAIREGGRTVGAGVVSKILK
CCCEEEECCCCEEEEEEECCCEEECCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
ARAKFERNKPHVNIGTIGHVDHGKTTLTAAITMTLAALGRAKAKKYDEIDQAPEEKARG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ITINTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPNIVVFLNKKDQLDDPELLELVELEVRELLSKYDFPGDDVPIVAGSALMALE
EEEHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHH
KMASEPKLIRGKDDWVDCIYSLMDAVDAYIPTPERAIDKPFLMAVEDVFSITGRGTVATG
HHCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEC
RIERGKVKVGETIELVGIRGTRSTTVTGLEMFQKSLDEGLAGDNIGVLLRGIKKEDVERG
CEECCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHEECCCHHHHHCC
MVLAKPGSITPHTQFEGEVYILSKEEGGRHTPFFAGYRPQFYVRTTDVTGTIVTFTDDEG
EEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCEEEEEEECCCEEEEEEECCCC
KSAEMVMPGDRIKMTVELINPIAIEDGMRFAIREGGRTVGAGVVSKILK
CCCEEEECCCCEEEEEEECCCEEECCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292; 7663757