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The map label for this gene is pulE [H]

Identifier: 37523453

GI number: 37523453

Start: 4090402

End: 4092267

Strand: Reverse

Name: pulE [H]

Synonym: gll3884

Alternate gene names: 37523453

Gene position: 4092267-4090402 (Counterclockwise)

Preceding gene: 37523454

Following gene: 37523452

Centisome position: 87.84

GC content: 63.83

Gene sequence:

>1866_bases
GTGACAGCCATGAAAAGTTCCCCATCCACCCGAACTCAAAATCGTATCGAACGCTTCATCGGCGCGGTGAGCGCCGGCGA
GACCGAACGCCAGGTTCTCGAAGAACTCTACCGCACCAGTGGTACGCGCTTTTTTCAGCTCGCCTGTCAGCAGGTAGCCG
ATGAAAAGCGCTTTTGGGAGCAGTGCGCCGCCGTCTTCTTTTCGGTGGACGGGATCGTGCGTGCAGAGCAGATCGATCAA
ATCGAAGTGCAGCAAATCGAGAAGAACTGGGCGAGCCTCGGGCACCTGGTCATCCCCCACGGAGGCCGCCGCTCGGTGCT
CACCGTAAGCCCCCTTACGGTCGCTGAGCAGGCCGAACTGCAGGCGCTACCGGTCTATGTCCCAGAGAAAATCGAGTTGT
TCAACCTGTTGCGCCGCCAGATTGCAAGCGGCAGCCAGGCCACGCTGCAGGCCACCTCCGCCAAATCGGCGGACAAGATT
CTCCGCTGGGGCGAGTCGCCCGCCGACAGCCTGATTATTCAGCTTTACGAAGTGCTGCTGGCCTACGAAGCGTCCGATGT
ACGGCTTTTTACCCGCGGCGGCCGGTGCGCCCACGTCGCTGCCCATATCGGCGAGGAGCGCGTGCTCATCAGCGAAGAAA
TCGGTGAACTCGATTGGGGAAGCGTCGATCTTGAAGGCCGCGGCCGCCAGATGTTCGCCCGCCTCGCCTCGGCGGCCGAC
TGCGAAATTTCCCAGCGGCGCATCCAGTACGGCAACGTCACCCTGGCCGATCGCAGCGGCCAGTTGCAGACGATGCGCCT
CGTGATGGCGCCCCTCGGTCCCGGCGACGGCGGCGACTGGGAAATCGGCTTGCGGGTGATCAGCCAGGCGGGGTTTATTC
CCCTGGAGCGCATGAACTTGCACCCGCGCATCTACCGCAGCATCACCGGTCGGTGGCCTACCCAGGAGGAGCCGCCTGAG
CAGGTCTTAAGCGAGATGGAGCGCATCGCCGACACCGAGATGCTGTGTCTGTTGGATCTGGAGACGGCCATCAGCCTGGT
GGCAGGTTCGATGAACTCCGGCAAGTCGATGCTGCTGCAGAATGTCGGGTTGCTGCGCGCCCGCCGCGGCCACCATGTGC
ACAGCTTCGAGAGCCCCATCGAACGGCTGGTCGAGGGCATTTTTCAATACGAAGTCACCGAGCAAAACGACTGGTTGGGC
TGGTCGCGTTTTGCCACGCGCAACGACCCGGGCGACATTATCTTTGGAGAGATCAACGACCGCGACACCGCCCAACGCAT
CCTCGGATTCGCCAACGGCAAACTGGTGCTCACCACGCTGCACACCAACCGGGCAGTGCGTTTTACCGAGCGGCTGCGCA
ACTTGCTGCGCGGCGAATCGGAGCGCGACGGCCACGCCCAGATCGCCGCCTTCGTCTCGACCACCGGCTATGTGTACTGC
CAGAGACTCCTGCCGCGGGTTTGCCCCCACTGCTCGACCGTCGAAGCGGTACCGGAGCCTCTGCGCGAGGCATTGCAATG
GCAGATCCGACTATGGCGGCGGCCCGGTCCCGGCTGCGGCCGGGGCACCTGCCGCAGCGAAGGACCGCTGTTGGGCTATC
TGGCCGACCGCGTTCCCCTCACCGAGGCCATCTATGTGCCGGCTTTGGCCGAGCACTTTTTGCGCCCCGAGATCACCGTC
TTTGAGTTGTTGGCAGCAGCCGAGGTTCAGCACCAGCAGACCTACCGGCGCACCGCCGCCTGGATGCTCGACGCCGAGCG
CCGCCACGGCGGGCTGGTGAGTTGGCAAAAAGTCGCACCCCATCTGCAGGAGGACCGGCAGGCGATGCGGCTGTTGTCGC
GGCGCGAGGAGGAGGTGACGCTGTGA

Upstream 100 bases:

>100_bases
CGGAACTGCTCGAAGCGGAAGCTCCGGCCGCCGACAGTCCCCGCAACCTGCCCCCTGAATTTGCCAGCCACAAACCCGCC
TCCGGGATCACGCCGCTCAA

Downstream 100 bases:

>100_bases
CCGTTGCCTCCTCCTGGCGCCGCTTTGAAATCAGGACCTGGAGTCAAAGAAAAACGCTGGACTTATTGCAGCAATACCTC
TGGAATGCGGCCCTGGGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTAMKSSPSTRTQNRIERFIGAVSAGETERQVLEELYRTSGTRFFQLACQQVADEKRFWEQCAAVFFSVDGIVRAEQIDQ
IEVQQIEKNWASLGHLVIPHGGRRSVLTVSPLTVAEQAELQALPVYVPEKIELFNLLRRQIASGSQATLQATSAKSADKI
LRWGESPADSLIIQLYEVLLAYEASDVRLFTRGGRCAHVAAHIGEERVLISEEIGELDWGSVDLEGRGRQMFARLASAAD
CEISQRRIQYGNVTLADRSGQLQTMRLVMAPLGPGDGGDWEIGLRVISQAGFIPLERMNLHPRIYRSITGRWPTQEEPPE
QVLSEMERIADTEMLCLLDLETAISLVAGSMNSGKSMLLQNVGLLRARRGHHVHSFESPIERLVEGIFQYEVTEQNDWLG
WSRFATRNDPGDIIFGEINDRDTAQRILGFANGKLVLTTLHTNRAVRFTERLRNLLRGESERDGHAQIAAFVSTTGYVYC
QRLLPRVCPHCSTVEAVPEPLREALQWQIRLWRRPGPGCGRGTCRSEGPLLGYLADRVPLTEAIYVPALAEHFLRPEITV
FELLAAAEVQHQQTYRRTAAWMLDAERRHGGLVSWQKVAPHLQEDRQAMRLLSRREEEVTL

Sequences:

>Translated_621_residues
MTAMKSSPSTRTQNRIERFIGAVSAGETERQVLEELYRTSGTRFFQLACQQVADEKRFWEQCAAVFFSVDGIVRAEQIDQ
IEVQQIEKNWASLGHLVIPHGGRRSVLTVSPLTVAEQAELQALPVYVPEKIELFNLLRRQIASGSQATLQATSAKSADKI
LRWGESPADSLIIQLYEVLLAYEASDVRLFTRGGRCAHVAAHIGEERVLISEEIGELDWGSVDLEGRGRQMFARLASAAD
CEISQRRIQYGNVTLADRSGQLQTMRLVMAPLGPGDGGDWEIGLRVISQAGFIPLERMNLHPRIYRSITGRWPTQEEPPE
QVLSEMERIADTEMLCLLDLETAISLVAGSMNSGKSMLLQNVGLLRARRGHHVHSFESPIERLVEGIFQYEVTEQNDWLG
WSRFATRNDPGDIIFGEINDRDTAQRILGFANGKLVLTTLHTNRAVRFTERLRNLLRGESERDGHAQIAAFVSTTGYVYC
QRLLPRVCPHCSTVEAVPEPLREALQWQIRLWRRPGPGCGRGTCRSEGPLLGYLADRVPLTEAIYVPALAEHFLRPEITV
FELLAAAEVQHQQTYRRTAAWMLDAERRHGGLVSWQKVAPHLQEDRQAMRLLSRREEEVTL
>Mature_620_residues
TAMKSSPSTRTQNRIERFIGAVSAGETERQVLEELYRTSGTRFFQLACQQVADEKRFWEQCAAVFFSVDGIVRAEQIDQI
EVQQIEKNWASLGHLVIPHGGRRSVLTVSPLTVAEQAELQALPVYVPEKIELFNLLRRQIASGSQATLQATSAKSADKIL
RWGESPADSLIIQLYEVLLAYEASDVRLFTRGGRCAHVAAHIGEERVLISEEIGELDWGSVDLEGRGRQMFARLASAADC
EISQRRIQYGNVTLADRSGQLQTMRLVMAPLGPGDGGDWEIGLRVISQAGFIPLERMNLHPRIYRSITGRWPTQEEPPEQ
VLSEMERIADTEMLCLLDLETAISLVAGSMNSGKSMLLQNVGLLRARRGHHVHSFESPIERLVEGIFQYEVTEQNDWLGW
SRFATRNDPGDIIFGEINDRDTAQRILGFANGKLVLTTLHTNRAVRFTERLRNLLRGESERDGHAQIAAFVSTTGYVYCQ
RLLPRVCPHCSTVEAVPEPLREALQWQIRLWRRPGPGCGRGTCRSEGPLLGYLADRVPLTEAIYVPALAEHFLRPEITVF
ELLAAAEVQHQQTYRRTAAWMLDAERRHGGLVSWQKVAPHLQEDRQAMRLLSRREEEVTL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=160, Percent_Identity=33.75, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786296, Length=179, Percent_Identity=30.1675977653631, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 69962; Mature: 69830

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMKSSPSTRTQNRIERFIGAVSAGETERQVLEELYRTSGTRFFQLACQQVADEKRFWE
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QCAAVFFSVDGIVRAEQIDQIEVQQIEKNWASLGHLVIPHGGRRSVLTVSPLTVAEQAEL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCCCHHHHCCC
QALPVYVPEKIELFNLLRRQIASGSQATLQATSAKSADKILRWGESPADSLIIQLYEVLL
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHH
AYEASDVRLFTRGGRCAHVAAHIGEERVLISEEIGELDWGSVDLEGRGRQMFARLASAAD
HHCCCCEEEEECCCCHHHHHHHCCCCCEEEHHHCCCCCCCCEEECCCHHHHHHHHHHHCC
CEISQRRIQYGNVTLADRSGQLQTMRLVMAPLGPGDGGDWEIGLRVISQAGFIPLERMNL
CHHHHHHHHCCCEEEECCCCCHHEEHEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHCCC
HPRIYRSITGRWPTQEEPPEQVLSEMERIADTEMLCLLDLETAISLVAGSMNSGKSMLLQ
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEEHHHHHHHHHCCCCCCHHHHHH
NVGLLRARRGHHVHSFESPIERLVEGIFQYEVTEQNDWLGWSRFATRNDPGDIIFGEIND
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCCCCCCCCEEEECCCC
RDTAQRILGFANGKLVLTTLHTNRAVRFTERLRNLLRGESERDGHAQIAAFVSTTGYVYC
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QRLLPRVCPHCSTVEAVPEPLREALQWQIRLWRRPGPGCGRGTCRSEGPLLGYLADRVPL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHCCCCC
TEAIYVPALAEHFLRPEITVFELLAAAEVQHQQTYRRTAAWMLDAERRHGGLVSWQKVAP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHCH
HLQEDRQAMRLLSRREEEVTL
HHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TAMKSSPSTRTQNRIERFIGAVSAGETERQVLEELYRTSGTRFFQLACQQVADEKRFWE
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QCAAVFFSVDGIVRAEQIDQIEVQQIEKNWASLGHLVIPHGGRRSVLTVSPLTVAEQAEL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCCCHHHHCCC
QALPVYVPEKIELFNLLRRQIASGSQATLQATSAKSADKILRWGESPADSLIIQLYEVLL
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHH
AYEASDVRLFTRGGRCAHVAAHIGEERVLISEEIGELDWGSVDLEGRGRQMFARLASAAD
HHCCCCEEEEECCCCHHHHHHHCCCCCEEEHHHCCCCCCCCEEECCCHHHHHHHHHHHCC
CEISQRRIQYGNVTLADRSGQLQTMRLVMAPLGPGDGGDWEIGLRVISQAGFIPLERMNL
CHHHHHHHHCCCEEEECCCCCHHEEHEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHCCC
HPRIYRSITGRWPTQEEPPEQVLSEMERIADTEMLCLLDLETAISLVAGSMNSGKSMLLQ
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEEHHHHHHHHHCCCCCCHHHHHH
NVGLLRARRGHHVHSFESPIERLVEGIFQYEVTEQNDWLGWSRFATRNDPGDIIFGEIND
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCCCCCCCCEEEECCCC
RDTAQRILGFANGKLVLTTLHTNRAVRFTERLRNLLRGESERDGHAQIAAFVSTTGYVYC
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QRLLPRVCPHCSTVEAVPEPLREALQWQIRLWRRPGPGCGRGTCRSEGPLLGYLADRVPL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHCCCCC
TEAIYVPALAEHFLRPEITVFELLAAAEVQHQQTYRRTAAWMLDAERRHGGLVSWQKVAP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHCH
HLQEDRQAMRLLSRREEEVTL
HHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]