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The map label for this gene is ispG

Identifier: 37523191

GI number: 37523191

Start: 3820687

End: 3821877

Strand: Reverse

Name: ispG

Synonym: gll3622

Alternate gene names: 37523191

Gene position: 3821877-3820687 (Counterclockwise)

Preceding gene: 37523193

Following gene: 37523190

Centisome position: 82.03

GC content: 65.58

Gene sequence:

>1191_bases
ATGCAAACCCTCGACCCGGTCACTCCCGCTCTGTTGCCTTTGCGCCGCGTCACCCGGCCGGTGCAGGTGGGGGCGCTCAC
CATCGGCGGCAACGCCCCGGTGGTTGTCCAATCGATGATCAACGAGGACACCCTCGACACCCCGGGGGCGGTGGCGGCCA
TCCGCGCCCTGCACAAAGCCGGCTGCGAGCTGGTGCGGGTCACGGTGCCGAGCCTGGCCCACGCCCGCGCCCTCGAGCGC
ATCCGCAAGACCCTCGAAGACACCTACCGGCCGGTGCCCCTGGTCGCCGACGTCCACCACGACGGCGGTCCCATCGCTCT
GGAAGTGGCCCGCCACGTCGACAAAGTGCGCATCAACCCGGGATTGTTCGTCTTTCGACGGGCGCGCTTGGGCGACTACA
CCGCCGAGGACGTCGAGCGCGCCCGCGCCCAGATCCGCGAGGAACTGAAGCCCCTGGTGGATGTCCTGGGTGCCCAGGAC
AAGGCGATGCGCATCGGTGTCAACCACGGCTCGCTCTCGGAGCGGATGCTCTATATCCATGGCGACACCCCCGAGGGCAT
GGTCCAATCGGCCGTCGAATTTGTCGAGATTTGCGAGCAGCTGGGCTATCACAATCTGGTAATTTCCCTCAAAGCCTCGC
GCGTGCCGGTGATGATCGCGGTCTATCGCCTGGCGGCCAAGCTGCTCGACGCCCGGGGGATGCACTATCCCTTTCACCTC
GGGGTCACCGAGGCGGGCGACGGCGAGTACGGCCGCATCAAATCGACCGCCGGGATCGCGACGCTGTTGGCTGACGGCAT
CGGCGATACGATTCGCGTTTCGCTCACCGAAGATCCGCTCAAAGAAATTCCGGTCTGCTACGGTATCCTGCAGGCCCTGG
GCCTCAGGCGGACGATGGTCGAGTACGTCGCCTGCCCCAGTTGCGGGCGGACGCTGTTTAACCTCGAGACCGTTCTGCAC
CGGGTGCGCGAGGCGACCCGCCACCTCACCGGCCTCAACATCGCCGTAATGGGCTGCATCGTCAACGGCCCCGGTGAGAT
GGCCGACGCGGATTATGGTTACGTGGGCAAGACCCCCGGTACGATCTCGCTGTATCGGGGCCGCGAGGAGATCAAAAAGG
TGCCCGAAGCTCGCGGCGTCGAGGCACTGGTTGATTTAATCAAGTCCGATGGCCGCTGGGTGGATCCGTAA

Upstream 100 bases:

>100_bases
AAGCCCGATCGGGATCAGTGGGCGCTTCTAGGTATTTTTTCTCACCTGATCCTCAGGCAAAATCGTGTCAATCTGAAAGC
AGCGCACCACTTCATTCACC

Downstream 100 bases:

>100_bases
ACGGGTTGCGCTCCGGTGTAGAGGCCGCAGTACGCGAGGGCGCCTTACAAGCGATTTTCAAGCAGCGGCGCCGTTTTTGC
TGTCCAAAGCGTGCCCAACA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MQTLDPVTPALLPLRRVTRPVQVGALTIGGNAPVVVQSMINEDTLDTPGAVAAIRALHKAGCELVRVTVPSLAHARALER
IRKTLEDTYRPVPLVADVHHDGGPIALEVARHVDKVRINPGLFVFRRARLGDYTAEDVERARAQIREELKPLVDVLGAQD
KAMRIGVNHGSLSERMLYIHGDTPEGMVQSAVEFVEICEQLGYHNLVISLKASRVPVMIAVYRLAAKLLDARGMHYPFHL
GVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEDPLKEIPVCYGILQALGLRRTMVEYVACPSCGRTLFNLETVLH
RVREATRHLTGLNIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIKKVPEARGVEALVDLIKSDGRWVDP

Sequences:

>Translated_396_residues
MQTLDPVTPALLPLRRVTRPVQVGALTIGGNAPVVVQSMINEDTLDTPGAVAAIRALHKAGCELVRVTVPSLAHARALER
IRKTLEDTYRPVPLVADVHHDGGPIALEVARHVDKVRINPGLFVFRRARLGDYTAEDVERARAQIREELKPLVDVLGAQD
KAMRIGVNHGSLSERMLYIHGDTPEGMVQSAVEFVEICEQLGYHNLVISLKASRVPVMIAVYRLAAKLLDARGMHYPFHL
GVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEDPLKEIPVCYGILQALGLRRTMVEYVACPSCGRTLFNLETVLH
RVREATRHLTGLNIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIKKVPEARGVEALVDLIKSDGRWVDP
>Mature_396_residues
MQTLDPVTPALLPLRRVTRPVQVGALTIGGNAPVVVQSMINEDTLDTPGAVAAIRALHKAGCELVRVTVPSLAHARALER
IRKTLEDTYRPVPLVADVHHDGGPIALEVARHVDKVRINPGLFVFRRARLGDYTAEDVERARAQIREELKPLVDVLGAQD
KAMRIGVNHGSLSERMLYIHGDTPEGMVQSAVEFVEICEQLGYHNLVISLKASRVPVMIAVYRLAAKLLDARGMHYPFHL
GVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEDPLKEIPVCYGILQALGLRRTMVEYVACPSCGRTLFNLETVLH
RVREATRHLTGLNIAVMGCIVNGPGEMADADYGYVGKTPGTISLYRGREEIKKVPEARGVEALVDLIKSDGRWVDP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=345, Percent_Identity=36.8115942028986, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_GLOVI (Q7NFA4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926568.1
- ProteinModelPortal:   Q7NFA4
- GeneID:   2602026
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3622
- NMPDR:   fig|251221.1.peg.3622
- HOGENOM:   HBG335271
- OMA:   INEDTMD
- ProtClustDB:   PRK00366
- BioCyc:   GVIO251221:GLL3622-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 43159; Mature: 43159

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLDPVTPALLPLRRVTRPVQVGALTIGGNAPVVVQSMINEDTLDTPGAVAAIRALHKA
CCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
GCELVRVTVPSLAHARALERIRKTLEDTYRPVPLVADVHHDGGPIALEVARHVDKVRINP
CCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHEECCC
GLFVFRRARLGDYTAEDVERARAQIREELKPLVDVLGAQDKAMRIGVNHGSLSERMLYIH
CCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
GDTPEGMVQSAVEFVEICEQLGYHNLVISLKASRVPVMIAVYRLAAKLLDARGMHYPFHL
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEE
GVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEDPLKEIPVCYGILQALGLRRTMV
CCEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EYVACPSCGRTLFNLETVLHRVREATRHLTGLNIAVMGCIVNGPGEMADADYGYVGKTPG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
TISLYRGREEIKKVPEARGVEALVDLIKSDGRWVDP
EEEEECCHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQTLDPVTPALLPLRRVTRPVQVGALTIGGNAPVVVQSMINEDTLDTPGAVAAIRALHKA
CCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
GCELVRVTVPSLAHARALERIRKTLEDTYRPVPLVADVHHDGGPIALEVARHVDKVRINP
CCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHEECCC
GLFVFRRARLGDYTAEDVERARAQIREELKPLVDVLGAQDKAMRIGVNHGSLSERMLYIH
CCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
GDTPEGMVQSAVEFVEICEQLGYHNLVISLKASRVPVMIAVYRLAAKLLDARGMHYPFHL
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEE
GVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEDPLKEIPVCYGILQALGLRRTMV
CCEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EYVACPSCGRTLFNLETVLHRVREATRHLTGLNIAVMGCIVNGPGEMADADYGYVGKTPG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
TISLYRGREEIKKVPEARGVEALVDLIKSDGRWVDP
EEEEECCHHHHHHCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292