Click here to switch to the map view.

The map label for this gene is ygcM [C]

Identifier: 37523148

GI number: 37523148

Start: 3771105

End: 3771935

Strand: Direct

Name: ygcM [C]

Synonym: glr3579

Alternate gene names: 37523148

Gene position: 3771105-3771935 (Clockwise)

Preceding gene: 37523147

Following gene: 37523152

Centisome position: 80.94

GC content: 60.53

Gene sequence:

>831_bases
ATGGGTTGCCTGATTGTGCGCCGTGCCCGCTTCAGTGCGGCTCATCGCTACTGGTTATCGGAACTAAGCGAAGCGGAAAA
CCGTTCGCGCTTCGGCCCCACCACCCGCATCCACGGGCACAACTACGTGCTGTTCGTCTCGATGCTGGGTCCGGTGGACG
AGTACGGCATGGTGCTTAACCTAAGCGACGTCAAGCACGTGATTAAGCGCGAAGTGACCGCCCAGCTCGACACGAATTTG
CTCAACGAAGCCTGGCCGGAACTTGCCGAGACTTTGCCCACCACCGAGCACCTCGCGCGCGTCATCTTTCACCGGCTTGT
GCCCCACCTGCCCGTGGTGCGGGTGCAGTTATTCGAATCGGACGACCTTTGGGCTGAGTATCAGGGAGAAGGCATGCAAG
CGTATCTGACCATTTCCGACCACTTCGCCGCCGCCCACCGGCTGGCCCTCGATTCGCTCTCGCTTGAAGAAAACACCGAG
ATTTACGGCCTGTGCGCCCGCCCGAACGGCCACGGCCACAACTACCACGTCGAAATTACCGTCAAGGGCGAAGTGGACGG
ACGCACCGGCATGCTGGTGGATCTGGCGGCATTACAGCAGATTCTCAAAGACAAGGTCCTGCTGCCCTTCGACCACACCT
TCCTCAATAAGGACGTGCCCTACTTCGCCGGGGTGGTGCCCACTGCCGAAAATATCGCCCTCCATATCCGTGACCTGCTC
GAAGAGCCGGTGCGGGCTTTGGGGGCGAGGCTCCACAAAGTGCGACTCATCGAGAGCCCGAACAACTCGGTCGAGGTCCA
TACCGAAAGCTACGCCCCGCTGGTGGGCTGA

Upstream 100 bases:

>100_bases
AGTCTGGAGTTTTTCCCGGAAGGCCCGAGGAAAACCGGCGGCAGCTAGCCCTGGTCGGTACCGTGTATCCTGATACAAGA
GGTATCGAATTGTTGCGACT

Downstream 100 bases:

>100_bases
CAAAAAAGCCTAGGCAGATGAACCGCACTCCCTGGTATCCAGCAGTTGTGCTGCTTGGCGCAAGTCTGTGGCAACTGGCA
CCTCCTTCGCAGATCACGTC

Product: hypothetical protein

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; Preq(0) Biosynthesis Protein Qued; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MGCLIVRRARFSAAHRYWLSELSEAENRSRFGPTTRIHGHNYVLFVSMLGPVDEYGMVLNLSDVKHVIKREVTAQLDTNL
LNEAWPELAETLPTTEHLARVIFHRLVPHLPVVRVQLFESDDLWAEYQGEGMQAYLTISDHFAAAHRLALDSLSLEENTE
IYGLCARPNGHGHNYHVEITVKGEVDGRTGMLVDLAALQQILKDKVLLPFDHTFLNKDVPYFAGVVPTAENIALHIRDLL
EEPVRALGARLHKVRLIESPNNSVEVHTESYAPLVG

Sequences:

>Translated_276_residues
MGCLIVRRARFSAAHRYWLSELSEAENRSRFGPTTRIHGHNYVLFVSMLGPVDEYGMVLNLSDVKHVIKREVTAQLDTNL
LNEAWPELAETLPTTEHLARVIFHRLVPHLPVVRVQLFESDDLWAEYQGEGMQAYLTISDHFAAAHRLALDSLSLEENTE
IYGLCARPNGHGHNYHVEITVKGEVDGRTGMLVDLAALQQILKDKVLLPFDHTFLNKDVPYFAGVVPTAENIALHIRDLL
EEPVRALGARLHKVRLIESPNNSVEVHTESYAPLVG
>Mature_275_residues
GCLIVRRARFSAAHRYWLSELSEAENRSRFGPTTRIHGHNYVLFVSMLGPVDEYGMVLNLSDVKHVIKREVTAQLDTNLL
NEAWPELAETLPTTEHLARVIFHRLVPHLPVVRVQLFESDDLWAEYQGEGMQAYLTISDHFAAAHRLALDSLSLEENTEI
YGLCARPNGHGHNYHVEITVKGEVDGRTGMLVDLAALQQILKDKVLLPFDHTFLNKDVPYFAGVVPTAENIALHIRDLLE
EPVRALGARLHKVRLIESPNNSVEVHTESYAPLVG

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=142, Percent_Identity=45.0704225352113, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI115532226, Length=123, Percent_Identity=43.0894308943089, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115532224, Length=123, Percent_Identity=43.0894308943089, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19550074, Length=131, Percent_Identity=40.4580152671756, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585378, Length=131, Percent_Identity=40.4580152671756, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 31106; Mature: 30975

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCLIVRRARFSAAHRYWLSELSEAENRSRFGPTTRIHGHNYVLFVSMLGPVDEYGMVLN
CCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCCCEEEE
LSDVKHVIKREVTAQLDTNLLNEAWPELAETLPTTEHLARVIFHRLVPHLPVVRVQLFES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECC
DDLWAEYQGEGMQAYLTISDHFAAAHRLALDSLSLEENTEIYGLCARPNGHGHNYHVEIT
CCCEEEECCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEE
VKGEVDGRTGMLVDLAALQQILKDKVLLPFDHTFLNKDVPYFAGVVPTAENIALHIRDLL
EEECCCCCCCCEEEHHHHHHHHHCCEECCCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHH
EEPVRALGARLHKVRLIESPNNSVEVHTESYAPLVG
HHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
GCLIVRRARFSAAHRYWLSELSEAENRSRFGPTTRIHGHNYVLFVSMLGPVDEYGMVLN
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCCCEEEE
LSDVKHVIKREVTAQLDTNLLNEAWPELAETLPTTEHLARVIFHRLVPHLPVVRVQLFES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECC
DDLWAEYQGEGMQAYLTISDHFAAAHRLALDSLSLEENTEIYGLCARPNGHGHNYHVEIT
CCCEEEECCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEE
VKGEVDGRTGMLVDLAALQQILKDKVLLPFDHTFLNKDVPYFAGVVPTAENIALHIRDLL
EEECCCCCCCCEEEHHHHHHHHHCCEECCCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHH
EEPVRALGARLHKVRLIESPNNSVEVHTESYAPLVG
HHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA