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The map label for this gene is leuB

Identifier: 37523120

GI number: 37523120

Start: 3746812

End: 3747900

Strand: Reverse

Name: leuB

Synonym: gvip479

Alternate gene names: 37523120

Gene position: 3747900-3746812 (Counterclockwise)

Preceding gene: 37523121

Following gene: 37523119

Centisome position: 80.44

GC content: 67.13

Gene sequence:

>1089_bases
TTGGGCGGAAGATACAAAATCGCGGTGCTGCCGGGCGACGGCATCGGCACTGAAATTGTCCAGGTGGGCGTGGCGATGCT
GGAGGCCGCCGCTCGCCGGTTCGATTTCGCATTCGACTTTGAAGAAGCGCCCATCGGGGGGGCGGCCATCGATGCCACCG
GCGAACCGCTCCCGGCTGCGACCCTCGAACTGTGCAAAGCGAGCGACGCGGTGTTTCTAGGGGCGGTGGGCGGCCCGCAG
TGGGACACCCTCCCGTCGGACAAACGCCCCGAGAAGGCTCTGCTCGGCCTGCGCGCCGGTCTGGGGCTATTTGCCAATTT
GCGCCCGGCGCGCATCCTGCCGCAACTCGTCGCCGCCTCGGCCCTCAAGCCGGAGGTGGTCGAAGGGGTGGACATTCTGG
TGGTGCGCGAACTGACGGGCGGCCTGTACTTCGGCATTCCGAAGGGCATCTTCCCGGAGAAAAAAGGCGGGCGCCGGGGG
GTGAACACGATGAGCTACAGCGCCTTTGAGATCGAGCGCATCGGCCGGGTGGCTTTTGAAGCGGCCCGGGAACGGCGGGG
CAAACTGTGTTCGGTCGATAAAGCCAACGTGCTCGAAGTCTCGCAACTGTGGCGCGAAGTGCTTGTGGGCCTTGCCCCCG
AGTACCCGGATGTCGAACTGACGCACATGTACGTGGATAACTGTGCGATGCAGCTGGTGCGCCGGCCCCGCCAGTTCGAC
ACGATCGTCACCGAGAACATGTTCGGCGACATTCTCTCAGACGAAGCGGCGATGCTCACAGGCTCGATCGGCATGCTGCC
CTCGGCGTCGCTGGGCAGCGGCGGCCCGGGGCTCTACGAGCCGGTGCACGGCTCCGCCCCGGACATCGCGGGCCAGGACA
AGGCCAACCCACTGGCCCAGGTGCTCTCGGGGGCGATGCTGCTGCGCCACAGCCTCCGGCAACCCGAGGCCGCCCAGGCT
GTCGAAAGGGCGGTGCAGACGGTCCTGGAGCAAGGCTACCGTACCGGCGACATCGCCGCCCCCGGCGCCCAAATCGTCGG
TTGCCGGGCGATGGGCGAGCAGCTGCTCGCCGCCTTCGAGCGTGCGTGA

Upstream 100 bases:

>100_bases
TATCCCCCTGCCCAGGTGCATGGTCGCGACGAAGATTGACTCGCCGATTGCAGGTGGGCGATGATGGCTTGCTCCCGTTA
AGCAGCAAAGGGTCAGAGCG

Downstream 100 bases:

>100_bases
GCGGGTTGGCTCAGATTTGAACGATGATTGCTGAGGTGATCGTTATTTGAGCGGTCTTGCTAAGATTTCAAAGCCCAGGC
GTGCCGGGCGAGCATTGGAG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MGGRYKIAVLPGDGIGTEIVQVGVAMLEAAARRFDFAFDFEEAPIGGAAIDATGEPLPAATLELCKASDAVFLGAVGGPQ
WDTLPSDKRPEKALLGLRAGLGLFANLRPARILPQLVAASALKPEVVEGVDILVVRELTGGLYFGIPKGIFPEKKGGRRG
VNTMSYSAFEIERIGRVAFEAARERRGKLCSVDKANVLEVSQLWREVLVGLAPEYPDVELTHMYVDNCAMQLVRRPRQFD
TIVTENMFGDILSDEAAMLTGSIGMLPSASLGSGGPGLYEPVHGSAPDIAGQDKANPLAQVLSGAMLLRHSLRQPEAAQA
VERAVQTVLEQGYRTGDIAAPGAQIVGCRAMGEQLLAAFERA

Sequences:

>Translated_362_residues
MGGRYKIAVLPGDGIGTEIVQVGVAMLEAAARRFDFAFDFEEAPIGGAAIDATGEPLPAATLELCKASDAVFLGAVGGPQ
WDTLPSDKRPEKALLGLRAGLGLFANLRPARILPQLVAASALKPEVVEGVDILVVRELTGGLYFGIPKGIFPEKKGGRRG
VNTMSYSAFEIERIGRVAFEAARERRGKLCSVDKANVLEVSQLWREVLVGLAPEYPDVELTHMYVDNCAMQLVRRPRQFD
TIVTENMFGDILSDEAAMLTGSIGMLPSASLGSGGPGLYEPVHGSAPDIAGQDKANPLAQVLSGAMLLRHSLRQPEAAQA
VERAVQTVLEQGYRTGDIAAPGAQIVGCRAMGEQLLAAFERA
>Mature_361_residues
GGRYKIAVLPGDGIGTEIVQVGVAMLEAAARRFDFAFDFEEAPIGGAAIDATGEPLPAATLELCKASDAVFLGAVGGPQW
DTLPSDKRPEKALLGLRAGLGLFANLRPARILPQLVAASALKPEVVEGVDILVVRELTGGLYFGIPKGIFPEKKGGRRGV
NTMSYSAFEIERIGRVAFEAARERRGKLCSVDKANVLEVSQLWREVLVGLAPEYPDVELTHMYVDNCAMQLVRRPRQFDT
IVTENMFGDILSDEAAMLTGSIGMLPSASLGSGGPGLYEPVHGSAPDIAGQDKANPLAQVLSGAMLLRHSLRQPEAAQAV
ERAVQTVLEQGYRTGDIAAPGAQIVGCRAMGEQLLAAFERA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=314, Percent_Identity=34.7133757961783, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4758582, Length=349, Percent_Identity=29.2263610315186, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI28178838, Length=326, Percent_Identity=29.7546012269939, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI28178816, Length=363, Percent_Identity=26.1707988980716, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI28178821, Length=314, Percent_Identity=28.0254777070064, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=34.7517730496454, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=54.4692737430168, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=36.2857142857143, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1787381, Length=401, Percent_Identity=26.6832917705736, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=33.0459770114943, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17505779, Length=344, Percent_Identity=29.3604651162791, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=29.2397660818713, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=26.8221574344023, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=45.0819672131148, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=33.8842975206612, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=345, Percent_Identity=33.9130434782609, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=345, Percent_Identity=31.0144927536232, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=33.5294117647059, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=33.5294117647059, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=30.6122448979592, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=28.1609195402299, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=28.1609195402299, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=28.1609195402299, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=28.1609195402299, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=27.1137026239067, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=27.1137026239067, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078639, Length=254, Percent_Identity=29.5275590551181, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130355, Length=231, Percent_Identity=26.4069264069264, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_GLOVI (Q7NFH4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926497.1
- ProteinModelPortal:   Q7NFH4
- SMR:   Q7NFH4
- GeneID:   2601955
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3551
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   GVIO251221:GLL3551-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38437; Mature: 38306

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 98-98 BINDING 108-108 BINDING 136-136 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGRYKIAVLPGDGIGTEIVQVGVAMLEAAARRFDFAFDFEEAPIGGAAIDATGEPLPAA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEECCCCCCCCHH
TLELCKASDAVFLGAVGGPQWDTLPSDKRPEKALLGLRAGLGLFANLRPARILPQLVAAS
HHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALKPEVVEGVDILVVRELTGGLYFGIPKGIFPEKKGGRRGVNTMSYSAFEIERIGRVAFE
CCCCHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AARERRGKLCSVDKANVLEVSQLWREVLVGLAPEYPDVELTHMYVDNCAMQLVRRPRQFD
HHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCHHHH
TIVTENMFGDILSDEAAMLTGSIGMLPSASLGSGGPGLYEPVHGSAPDIAGQDKANPLAQ
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSGAMLLRHSLRQPEAAQAVERAVQTVLEQGYRTGDIAAPGAQIVGCRAMGEQLLAAFE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHH
RA
CC
>Mature Secondary Structure 
GGRYKIAVLPGDGIGTEIVQVGVAMLEAAARRFDFAFDFEEAPIGGAAIDATGEPLPAA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEECCCCCCCCHH
TLELCKASDAVFLGAVGGPQWDTLPSDKRPEKALLGLRAGLGLFANLRPARILPQLVAAS
HHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALKPEVVEGVDILVVRELTGGLYFGIPKGIFPEKKGGRRGVNTMSYSAFEIERIGRVAFE
CCCCHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AARERRGKLCSVDKANVLEVSQLWREVLVGLAPEYPDVELTHMYVDNCAMQLVRRPRQFD
HHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCHHHH
TIVTENMFGDILSDEAAMLTGSIGMLPSASLGSGGPGLYEPVHGSAPDIAGQDKANPLAQ
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSGAMLLRHSLRQPEAAQAVERAVQTVLEQGYRTGDIAAPGAQIVGCRAMGEQLLAAFE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHH
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292