Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 37523116

GI number: 37523116

Start: 3744521

End: 3745012

Strand: Direct

Name: ispF

Synonym: glr3547

Alternate gene names: 37523116

Gene position: 3744521-3745012 (Clockwise)

Preceding gene: 37523115

Following gene: 37523118

Centisome position: 80.37

GC content: 66.06

Gene sequence:

>492_bases
GTGGCAGTGGGAGGGTTTGAAATGCGCATCGGCAACGGCTACGACTTTCACCGCCTGGTCGAAGGGCGGCGCTTGATCTT
GGGGGGTGTCGAGATTCCCTACCGCCTGGGCCTGCTCGGTCACAGCGACGCAGATCTGCTCACCCACGCGATCACCGACG
CCCTTTTGGGGGCCGCCTGCCTGGGCGATATCGGCCGACACTTCCCGCCGGGCGATCCCCAGTGGCAGGACGTCTCGAGT
CTGCTGCTGCTTTCGAAGGTGCTCGAACTGGTGCGCGGGCGCGGTCTGCGGCTTTCGAACGTCGATGCGGTGGTGGTGGC
GGAGCGGCCGAAGCTCGCTCCCCATATCCCGGCCATCCGCCAGAGTCTGGCGGGCGCATTGGGTTTACCCCTCGACCGAT
TGAGCGTCAAGGCCACGACCAACGAGGGCCTCGGCCCCGTGGGCGAAGGCCTTGCTATGGCCTGTCACGCGGTGGTGCTC
CTCGAAGAATAG

Upstream 100 bases:

>100_bases
GCCGTTCTCCCCGACTGCGGACACCTGCCCCAACTGGAGTGCCCGGACGCTTTTCACCACGAATTGCTCGCCTTTGCGGA
GGTGTTTCGCCCCAGAATGG

Downstream 100 bases:

>100_bases
GCAGGCGCTACACCTGGGCGGCCACTTCCACCGCCGGGCGGTGGCTGGCGCGCACCCCTTCGATCGCCTCGGCGTAGCGG
GCCGGGCCGACCCCAAACAC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MAVGGFEMRIGNGYDFHRLVEGRRLILGGVEIPYRLGLLGHSDADLLTHAITDALLGAACLGDIGRHFPPGDPQWQDVSS
LLLLSKVLELVRGRGLRLSNVDAVVVAERPKLAPHIPAIRQSLAGALGLPLDRLSVKATTNEGLGPVGEGLAMACHAVVL
LEE

Sequences:

>Translated_163_residues
MAVGGFEMRIGNGYDFHRLVEGRRLILGGVEIPYRLGLLGHSDADLLTHAITDALLGAACLGDIGRHFPPGDPQWQDVSS
LLLLSKVLELVRGRGLRLSNVDAVVVAERPKLAPHIPAIRQSLAGALGLPLDRLSVKATTNEGLGPVGEGLAMACHAVVL
LEE
>Mature_162_residues
AVGGFEMRIGNGYDFHRLVEGRRLILGGVEIPYRLGLLGHSDADLLTHAITDALLGAACLGDIGRHFPPGDPQWQDVSSL
LLLSKVLELVRGRGLRLSNVDAVVVAERPKLAPHIPAIRQSLAGALGLPLDRLSVKATTNEGLGPVGEGLAMACHAVVLL
EE

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=49.3506493506493, Blast_Score=141, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_GLOVI (Q7NFH8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926493.1
- ProteinModelPortal:   Q7NFH8
- SMR:   Q7NFH8
- GeneID:   2601951
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr3547
- NMPDR:   fig|251221.1.peg.3547
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- BioCyc:   GVIO251221:GLR3547-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17263; Mature: 17132

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGGFEMRIGNGYDFHRLVEGRRLILGGVEIPYRLGLLGHSDADLLTHAITDALLGAAC
CCCCCEEEEECCCCCHHHHHCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
LGDIGRHFPPGDPQWQDVSSLLLLSKVLELVRGRGLRLSNVDAVVVAERPKLAPHIPAIR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCHHHH
QSLAGALGLPLDRLSVKATTNEGLGPVGEGLAMACHAVVLLEE
HHHHHHHCCCHHHEEEEECCCCCCCCCHHHHHHHHHHEEEECC
>Mature Secondary Structure 
AVGGFEMRIGNGYDFHRLVEGRRLILGGVEIPYRLGLLGHSDADLLTHAITDALLGAAC
CCCCEEEEECCCCCHHHHHCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
LGDIGRHFPPGDPQWQDVSSLLLLSKVLELVRGRGLRLSNVDAVVVAERPKLAPHIPAIR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCHHHH
QSLAGALGLPLDRLSVKATTNEGLGPVGEGLAMACHAVVLLEE
HHHHHHHCCCHHHEEEEECCCCCCCCCHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292