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The map label for this gene is murE

Identifier: 37523061

GI number: 37523061

Start: 3701841

End: 3703310

Strand: Reverse

Name: murE

Synonym: gvip474

Alternate gene names: 37523061

Gene position: 3703310-3701841 (Counterclockwise)

Preceding gene: 37523062

Following gene: 37523060

Centisome position: 79.49

GC content: 65.51

Gene sequence:

>1470_bases
GTGCCTGCCATGCGTTTGCACGAACTGCTTGCCCGAACCGGGCTCGATACCCGCAACCTTCCCGACATCGACATTGCCGG
TCTGAGCACCGATTCGCGCCAGGTGCAGCCGGGGGACTTGTTTATTGGTCTTCCGGGTACGCGGGTGGACGGCAGTGAAT
TCTGGCCGCAGGCGATTGCCGGGGGAGCGGCGGGGCTGGTGATTTCTGAGAACGCCCGGGGGGTGGAGGCGGCGGTGCCG
GTGATCCGGGTGCCGGACGTGGTGGGTACCTGCGCCCGGCTCGCGTCTGCCTACTACGACTTTCCAGCCCGCAAACTAAC
CCTCGCCGGGGTGACCGGCACCAACGGCAAGACGACGACAACCCATTTGATCGAGAATCTATTGCAAGCCCAGGCACCCA
CGGGTCTGGTGGGCACGCTCTACAGCCGCTGGCCGGGCCAGAGCCAGGAGGCCCGGCACACCACGCCCTTTGCGCTTGAG
ATCCAGAAGTTGCTTGCTCAGATGGTGGAAGCGGGCTGCCAGTACGGGGTGATGGAGGTCAGCTCCCACGCCCTGGCCCA
GCAGCGGGTGGCGGGTTGCCGCTTCGAGGCCGCGGTCTTCACCAACCTCACCCAGGACCACCTCGACTTCCACCCGGACA
TGGAAAGTTACTTCCAGGCGAAGGCGACCCTGTTCAGTCCCGAGTACCGCAGCGGGCGGGCGGTGATCAACGCCGACGAC
CCGTGGGGGGTGCGCCTTGCAGCCGCGTACGACCAGGTGTGGACCTACAGCTTTCAGCCCGGCGCGGACATTTATCCAGA
AGATGCCGTGTTCACCCCGGAGGGCATCCGCGGCACCCTCGTCACCCCGGTGGGCCGCGCCGCCTTTACCAGCCCCCTGG
TGGGCCAGTTCAACCTGGCTAATCTGCTGGCGGCGGTCGGGGCCACCCTGGCTTTGGGAATCGACCTGGAGGCGATCGCA
GCGGGGCTGTCGGGTTTTGGCGGCGTACCTGGCCGCATGGAGCGGGTGAGCGGTTCAGACGACGATATCGCCGTGATTGT
TGATTACGCCCACACCCCCGACGGCCTGCGCAAACTGCTGGAGGCCACCCGGCCTTTCGTGCGCGGGCGGCTGATTTGCG
TATTCGGCTGCGGCGGGGATCGCGATCGCACCAAACGCCCCCAGATGGGCCGCATCGCCGCCGAGCTGTCGGATCTGCCG
GTGGTCACCTCCGACAATCCCCGCACCGAGAATCCGGAAGCGATCTTGGACGATATCCTGGCGGGCATTCCGGTGGGAGT
GTCGCCGACCGTGGAAGTGGACCGCCGCCGGGCGATATTGCAGGCACTGCTCGAAGCAAAGGCAGGCGATTGCGTGGTGA
TCGCAGGGAAAGGCCATGAGGATTATCAGATCCTGGGGACGACAAAAATCCACTTCGACGATCGCGAACAAGCCCGCGAA
GCGCTTTCCAAGCGCCGCAGCCGAGGTTGA

Upstream 100 bases:

>100_bases
TTCGAAGCGATCGCCAAAAACCTGGATACGGCCCTTGCCCGCCTCAAGTAGCGAACCGGGCAAAAGCTGCTACTCTAAAT
TCGACCTCCAGGGTACGAAG

Downstream 100 bases:

>100_bases
AAAAGCCTGGCAAGTTAGGAAACTACTTTTACTAAATCGGGCATAGAGTTTGTTGGAGATTCGACCGTGGATCTAGAGTT
TTTGAGCAACAATTTATTAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MPAMRLHELLARTGLDTRNLPDIDIAGLSTDSRQVQPGDLFIGLPGTRVDGSEFWPQAIAGGAAGLVISENARGVEAAVP
VIRVPDVVGTCARLASAYYDFPARKLTLAGVTGTNGKTTTTHLIENLLQAQAPTGLVGTLYSRWPGQSQEARHTTPFALE
IQKLLAQMVEAGCQYGVMEVSSHALAQQRVAGCRFEAAVFTNLTQDHLDFHPDMESYFQAKATLFSPEYRSGRAVINADD
PWGVRLAAAYDQVWTYSFQPGADIYPEDAVFTPEGIRGTLVTPVGRAAFTSPLVGQFNLANLLAAVGATLALGIDLEAIA
AGLSGFGGVPGRMERVSGSDDDIAVIVDYAHTPDGLRKLLEATRPFVRGRLICVFGCGGDRDRTKRPQMGRIAAELSDLP
VVTSDNPRTENPEAILDDILAGIPVGVSPTVEVDRRRAILQALLEAKAGDCVVIAGKGHEDYQILGTTKIHFDDREQARE
ALSKRRSRG

Sequences:

>Translated_489_residues
MPAMRLHELLARTGLDTRNLPDIDIAGLSTDSRQVQPGDLFIGLPGTRVDGSEFWPQAIAGGAAGLVISENARGVEAAVP
VIRVPDVVGTCARLASAYYDFPARKLTLAGVTGTNGKTTTTHLIENLLQAQAPTGLVGTLYSRWPGQSQEARHTTPFALE
IQKLLAQMVEAGCQYGVMEVSSHALAQQRVAGCRFEAAVFTNLTQDHLDFHPDMESYFQAKATLFSPEYRSGRAVINADD
PWGVRLAAAYDQVWTYSFQPGADIYPEDAVFTPEGIRGTLVTPVGRAAFTSPLVGQFNLANLLAAVGATLALGIDLEAIA
AGLSGFGGVPGRMERVSGSDDDIAVIVDYAHTPDGLRKLLEATRPFVRGRLICVFGCGGDRDRTKRPQMGRIAAELSDLP
VVTSDNPRTENPEAILDDILAGIPVGVSPTVEVDRRRAILQALLEAKAGDCVVIAGKGHEDYQILGTTKIHFDDREQARE
ALSKRRSRG
>Mature_488_residues
PAMRLHELLARTGLDTRNLPDIDIAGLSTDSRQVQPGDLFIGLPGTRVDGSEFWPQAIAGGAAGLVISENARGVEAAVPV
IRVPDVVGTCARLASAYYDFPARKLTLAGVTGTNGKTTTTHLIENLLQAQAPTGLVGTLYSRWPGQSQEARHTTPFALEI
QKLLAQMVEAGCQYGVMEVSSHALAQQRVAGCRFEAAVFTNLTQDHLDFHPDMESYFQAKATLFSPEYRSGRAVINADDP
WGVRLAAAYDQVWTYSFQPGADIYPEDAVFTPEGIRGTLVTPVGRAAFTSPLVGQFNLANLLAAVGATLALGIDLEAIAA
GLSGFGGVPGRMERVSGSDDDIAVIVDYAHTPDGLRKLLEATRPFVRGRLICVFGCGGDRDRTKRPQMGRIAAELSDLPV
VTSDNPRTENPEAILDDILAGIPVGVSPTVEVDRRRAILQALLEAKAGDCVVIAGKGHEDYQILGTTKIHFDDREQAREA
LSKRRSRG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=462, Percent_Identity=41.3419913419913, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1786274, Length=318, Percent_Identity=29.559748427673, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_GLOVI (Q7NFN2)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926438.1
- ProteinModelPortal:   Q7NFN2
- SMR:   Q7NFN2
- GeneID:   2601896
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3492
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   PRK00139
- BioCyc:   GVIO251221:GLL3492-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 52455; Mature: 52324

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 181-181 BINDING 187-187 BINDING 189-189 BINDING 381-381 BINDING 456-456 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAMRLHELLARTGLDTRNLPDIDIAGLSTDSRQVQPGDLFIGLPGTRVDGSEFWPQAIA
CCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCHHHCHHHHC
GGAAGLVISENARGVEAAVPVIRVPDVVGTCARLASAYYDFPARKLTLAGVTGTNGKTTT
CCCCEEEEECCCCCHHHHCCEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHH
THLIENLLQAQAPTGLVGTLYSRWPGQSQEARHTTPFALEIQKLLAQMVEAGCQYGVMEV
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
SSHALAQQRVAGCRFEAAVFTNLTQDHLDFHPDMESYFQAKATLFSPEYRSGRAVINADD
HHHHHHHHHHHCCEEHHHHHHCCCHHHCCCCCCHHHHHHHHHHEECCCCCCCCEEEECCC
PWGVRLAAAYDQVWTYSFQPGADIYPEDAVFTPEGIRGTLVTPVGRAAFTSPLVGQFNLA
CCCEEEEEEHHHHEEEECCCCCCCCCCCCEECCCCCCEEEECCCCHHHHCCCCCCCCCHH
NLLAAVGATLALGIDLEAIAAGLSGFGGVPGRMERVSGSDDDIAVIVDYAHTPDGLRKLL
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHH
EATRPFVRGRLICVFGCGGDRDRTKRPQMGRIAAELSDLPVVTSDNPRTENPEAILDDIL
HHHCHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
AGIPVGVSPTVEVDRRRAILQALLEAKAGDCVVIAGKGHEDYQILGTTKIHFDDREQARE
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEECCHHHHHH
ALSKRRSRG
HHHHHHCCC
>Mature Secondary Structure 
PAMRLHELLARTGLDTRNLPDIDIAGLSTDSRQVQPGDLFIGLPGTRVDGSEFWPQAIA
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCHHHCHHHHC
GGAAGLVISENARGVEAAVPVIRVPDVVGTCARLASAYYDFPARKLTLAGVTGTNGKTTT
CCCCEEEEECCCCCHHHHCCEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHH
THLIENLLQAQAPTGLVGTLYSRWPGQSQEARHTTPFALEIQKLLAQMVEAGCQYGVMEV
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
SSHALAQQRVAGCRFEAAVFTNLTQDHLDFHPDMESYFQAKATLFSPEYRSGRAVINADD
HHHHHHHHHHHCCEEHHHHHHCCCHHHCCCCCCHHHHHHHHHHEECCCCCCCCEEEECCC
PWGVRLAAAYDQVWTYSFQPGADIYPEDAVFTPEGIRGTLVTPVGRAAFTSPLVGQFNLA
CCCEEEEEEHHHHEEEECCCCCCCCCCCCEECCCCCCEEEECCCCHHHHCCCCCCCCCHH
NLLAAVGATLALGIDLEAIAAGLSGFGGVPGRMERVSGSDDDIAVIVDYAHTPDGLRKLL
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHH
EATRPFVRGRLICVFGCGGDRDRTKRPQMGRIAAELSDLPVVTSDNPRTENPEAILDDIL
HHHCHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
AGIPVGVSPTVEVDRRRAILQALLEAKAGDCVVIAGKGHEDYQILGTTKIHFDDREQARE
HCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEECCHHHHHH
ALSKRRSRG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292