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The map label for this gene is rfbM [H]

Identifier: 37523049

GI number: 37523049

Start: 3687900

End: 3688841

Strand: Direct

Name: rfbM [H]

Synonym: gvip473

Alternate gene names: 37523049

Gene position: 3687900-3688841 (Clockwise)

Preceding gene: 37523047

Following gene: 37523050

Centisome position: 79.16

GC content: 65.71

Gene sequence:

>942_bases
ATGCAAGCGGTAATTGTGGCCGGGGGCAAAGGAACGCGCCTGCGACCTTTGACCTATCGAACGCCTAAACCGATGCTGCC
CATCTTCGAGCGGCCCTTCCTGTGCCTGCTGGTGGAGCGCTGCCGGGCGGCAGGAATCGTCGACATCTTGATGAACGTGC
ATTATCAGGCAGGACAAATTCAGGATTACTTCGGCGACGGCGGCGCCTTTGGTGTGCGCATCCGCTACAGTGTCGAGCAG
AATCCACTGGATACCGCCGGGGCGGTGAAGCTGGCCGAGCCCTATTTCACCGGCGCGCCGCTGGTGGTCTTCAACGCCGA
TATCCTTACCGATCTGGACTTGACGGCTCTGGTCGCAGCGCACCGCCAGGCCGGGGCGGCGGCCACCCTCGCCCTCACCC
GCGTCCTCGACCCGACCGCCTTCGGATTGGTGCAATTGACCGACCACAACCAGGTGGCGGCCTTTCGCGAGAAACCGACC
GCCGAGGAAGCGGCCCGGCTGGGCATCGACACCATCAACGCCGGCACCTACGTACTCGATCCGCGCATCTTTGCCGATGT
GCCTGCCCAAACCCCCTGGAGCTTCGAGCGCCAGTTGTTTCCGAACTTGCTCGCTCAGCGGCAGAAGGTGCTGGGCTTCG
TCTCGGACACCTACTGGCTCGACATCGGCAACCCGGCCAAGTACTGGCAGGCCCACCTCGATATCTTGAGCGGCCGGATG
CCCCTCCCCCTTCCGGCGCAGCAGATCGCCCCGGGGATCTGGGTGGCCGAAGGAGCCGCGGTCGATGCGGCCGCCCGGCT
CGAAGCACCCTGCTACATCGGCGGGTGCTGCTGTGTCGGCCCTGAAGCGGCGATTGCCCCCGGCACGGTGCTGGGCAGTG
CCTCGCTGGTCAATCGGCCCCTGAGCCCCGGCATCTACCCGCCCGGGACGATGGTGCCGTAG

Upstream 100 bases:

>100_bases
TGGTTTTGCTCACTCAAGGCCTCGTCGAGAACATTGACGTTGACATCGTTGCAAATTATATCGCAAGGTAAATTCCGAAT
CGCATCCGGAGCGACAAACA

Downstream 100 bases:

>100_bases
CTGAAGCGCATCAGCAGGTGGGCCATCGATCCGGCCAGCACACAACTGGTTCCTCCCAAAATTTTCCACGGCCGGCTCAA
CTCCGAAAACGGATTGATCC

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MQAVIVAGGKGTRLRPLTYRTPKPMLPIFERPFLCLLVERCRAAGIVDILMNVHYQAGQIQDYFGDGGAFGVRIRYSVEQ
NPLDTAGAVKLAEPYFTGAPLVVFNADILTDLDLTALVAAHRQAGAAATLALTRVLDPTAFGLVQLTDHNQVAAFREKPT
AEEAARLGIDTINAGTYVLDPRIFADVPAQTPWSFERQLFPNLLAQRQKVLGFVSDTYWLDIGNPAKYWQAHLDILSGRM
PLPLPAQQIAPGIWVAEGAAVDAAARLEAPCYIGGCCCVGPEAAIAPGTVLGSASLVNRPLSPGIYPPGTMVP

Sequences:

>Translated_313_residues
MQAVIVAGGKGTRLRPLTYRTPKPMLPIFERPFLCLLVERCRAAGIVDILMNVHYQAGQIQDYFGDGGAFGVRIRYSVEQ
NPLDTAGAVKLAEPYFTGAPLVVFNADILTDLDLTALVAAHRQAGAAATLALTRVLDPTAFGLVQLTDHNQVAAFREKPT
AEEAARLGIDTINAGTYVLDPRIFADVPAQTPWSFERQLFPNLLAQRQKVLGFVSDTYWLDIGNPAKYWQAHLDILSGRM
PLPLPAQQIAPGIWVAEGAAVDAAARLEAPCYIGGCCCVGPEAAIAPGTVLGSASLVNRPLSPGIYPPGTMVP
>Mature_313_residues
MQAVIVAGGKGTRLRPLTYRTPKPMLPIFERPFLCLLVERCRAAGIVDILMNVHYQAGQIQDYFGDGGAFGVRIRYSVEQ
NPLDTAGAVKLAEPYFTGAPLVVFNADILTDLDLTALVAAHRQAGAAATLALTRVLDPTAFGLVQLTDHNQVAAFREKPT
AEEAARLGIDTINAGTYVLDPRIFADVPAQTPWSFERQLFPNLLAQRQKVLGFVSDTYWLDIGNPAKYWQAHLDILSGRM
PLPLPAQQIAPGIWVAEGAAVDAAARLEAPCYIGGCCCVGPEAAIAPGTVLGSASLVNRPLSPGIYPPGTMVP

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=304, Percent_Identity=31.5789473684211, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI11761619, Length=304, Percent_Identity=31.5789473684211, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI31881779, Length=332, Percent_Identity=25, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI45447090, Length=332, Percent_Identity=25, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1790224, Length=161, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788351, Length=160, Percent_Identity=30.625, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788355, Length=273, Percent_Identity=25.2747252747253, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=311, Percent_Identity=29.903536977492, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17509979, Length=304, Percent_Identity=25.9868421052632, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17509981, Length=293, Percent_Identity=25.2559726962457, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=315, Percent_Identity=30.4761904761905, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355443, Length=300, Percent_Identity=29.6666666666667, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24644084, Length=300, Percent_Identity=29.6666666666667, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24653912, Length=200, Percent_Identity=31.5, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 33531; Mature: 33531

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVIVAGGKGTRLRPLTYRTPKPMLPIFERPFLCLLVERCRAAGIVDILMNVHYQAGQI
CCEEEEECCCCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCHHHHHHHCCCCCCCC
QDYFGDGGAFGVRIRYSVEQNPLDTAGAVKLAEPYFTGAPLVVFNADILTDLDLTALVAA
HHHCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECHHHCCCHHHHHHHH
HRQAGAAATLALTRVLDPTAFGLVQLTDHNQVAAFREKPTAEEAARLGIDTINAGTYVLD
HHHCCHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHCCCCHHHHHHCCCEEECCCEEEEC
PRIFADVPAQTPWSFERQLFPNLLAQRQKVLGFVSDTYWLDIGNPAKYWQAHLDILSGRM
CHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHCCCC
PLPLPAQQIAPGIWVAEGAAVDAAARLEAPCYIGGCCCVGPEAAIAPGTVLGSASLVNRP
CCCCCHHHHCCCEEEECCCCCCHHHHCCCCEEECCEEECCCCCCCCCCCEECCHHHHCCC
LSPGIYPPGTMVP
CCCCCCCCCCCCC
>Mature Secondary Structure
MQAVIVAGGKGTRLRPLTYRTPKPMLPIFERPFLCLLVERCRAAGIVDILMNVHYQAGQI
CCEEEEECCCCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCHHHHHHHCCCCCCCC
QDYFGDGGAFGVRIRYSVEQNPLDTAGAVKLAEPYFTGAPLVVFNADILTDLDLTALVAA
HHHCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECHHHCCCHHHHHHHH
HRQAGAAATLALTRVLDPTAFGLVQLTDHNQVAAFREKPTAEEAARLGIDTINAGTYVLD
HHHCCHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHCCCCHHHHHHCCCEEECCCEEEEC
PRIFADVPAQTPWSFERQLFPNLLAQRQKVLGFVSDTYWLDIGNPAKYWQAHLDILSGRM
CHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHCCCC
PLPLPAQQIAPGIWVAEGAAVDAAARLEAPCYIGGCCCVGPEAAIAPGTVLGSASLVNRP
CCCCCHHHHCCCEEEECCCCCCHHHHCCCCEEECCEEECCCCCCCCCCCEECCHHHHCCC
LSPGIYPPGTMVP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]