Click here to switch to the map view.

The map label for this gene is gloB

Identifier: 37522908

GI number: 37522908

Start: 3543742

End: 3544500

Strand: Reverse

Name: gloB

Synonym: gll3339

Alternate gene names: 37522908

Gene position: 3544500-3543742 (Counterclockwise)

Preceding gene: 37522910

Following gene: 37522906

Centisome position: 76.08

GC content: 66.53

Gene sequence:

>759_bases
ATGCTGGTCCACCGCCTCAACGCCCTGAAGGACAACTATGTGTTCGTGCTGGAGGACGAAGCGGCCAGAACGGCGGCCGT
CGTCGATCCCGCCGAGGCCCGGCCGGTGCTCGAAGCGCTCGTCCGCCTCGGGCTCAAGCTCGTCGCCATCTTCAATACCC
ACCACCACCACGACCACGTCGGGGGCAACCGCGAACTGCTCGAAGCCTATCCCGGCATCGCGGTCTACGCTTCGCGCCGG
GACCGCGGCCGCATCCCCGGCCAGACCGTCGAACTCGAAGACGGCGACACGGTCGCCTTCGGCTGTGAGCGGGCGCGCGT
GATCTTCGTTCCCGGCCACACCCACGGCCATATCGCTTACCACTTTGCGGGTTGCGGGCACCTCTTCTGCGGAGATACGC
TTTTTGCCGGGGGCTGCGGCCGTCTGTTCGAGGGTACCGCCCGGCAGATGCAGCACTCGCTGGGCCGTCTGCGCGAACTG
CCGGGGGAAACCCAAGTCTGGTGCGCCCACGAGTACACCCTGGGCAACCTGCGCTTTGCGCACACCCTCGAACCGGACAA
CGCCGAACTGGCGGAGCGGCTGCGCACCGTCGAAGTGCTTCGGGCTCGAAAAATAGCGACGGTTCCCTCGACAATGGCCG
CGGAGCGGGCGACCAATCCTTTCTTGCGCTGGGAAAGCCGTCAATTACAACGGGCGGCGGGGGCGATCGAGCCGGAGGAG
GTGTTCGCCCACCTCCGGGCTCTCAAGGACCGCTTTTAG

Upstream 100 bases:

>100_bases
TTTTGACAAGGGAATCGATTTTTTCGTGTGTTGACTGGGACATGGTTTCAAGCTCCTGGCCATCAATACTTGATCTTAAC
GCAGCAATCCGGGAGGTCCG

Downstream 100 bases:

>100_bases
TAGCGGTTGGCCCGGCGGGTCAAGATCATCGCGAGGGTCTCGGCATCGTCGAGAGCCGGTTCGCGGTGGGCCTCGCAGGG
AATCACCTGGCTGGTGATGC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLVHRLNALKDNYVFVLEDEAARTAAVVDPAEARPVLEALVRLGLKLVAIFNTHHHHDHVGGNRELLEAYPGIAVYASRR
DRGRIPGQTVELEDGDTVAFGCERARVIFVPGHTHGHIAYHFAGCGHLFCGDTLFAGGCGRLFEGTARQMQHSLGRLREL
PGETQVWCAHEYTLGNLRFAHTLEPDNAELAERLRTVEVLRARKIATVPSTMAAERATNPFLRWESRQLQRAAGAIEPEE
VFAHLRALKDRF

Sequences:

>Translated_252_residues
MLVHRLNALKDNYVFVLEDEAARTAAVVDPAEARPVLEALVRLGLKLVAIFNTHHHHDHVGGNRELLEAYPGIAVYASRR
DRGRIPGQTVELEDGDTVAFGCERARVIFVPGHTHGHIAYHFAGCGHLFCGDTLFAGGCGRLFEGTARQMQHSLGRLREL
PGETQVWCAHEYTLGNLRFAHTLEPDNAELAERLRTVEVLRARKIATVPSTMAAERATNPFLRWESRQLQRAAGAIEPEE
VFAHLRALKDRF
>Mature_252_residues
MLVHRLNALKDNYVFVLEDEAARTAAVVDPAEARPVLEALVRLGLKLVAIFNTHHHHDHVGGNRELLEAYPGIAVYASRR
DRGRIPGQTVELEDGDTVAFGCERARVIFVPGHTHGHIAYHFAGCGHLFCGDTLFAGGCGRLFEGTARQMQHSLGRLREL
PGETQVWCAHEYTLGNLRFAHTLEPDNAELAERLRTVEVLRARKIATVPSTMAAERATNPFLRWESRQLQRAAGAIEPEE
VFAHLRALKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=258, Percent_Identity=38.3720930232558, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI94538322, Length=258, Percent_Identity=38.3720930232558, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI14150041, Length=257, Percent_Identity=38.9105058365759, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI116642887, Length=234, Percent_Identity=40.1709401709402, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI21703352, Length=259, Percent_Identity=38.2239382239382, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI46361987, Length=199, Percent_Identity=41.2060301507538, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI41327741, Length=193, Percent_Identity=30.5699481865285, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786406, Length=256, Percent_Identity=46.484375, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1787158, Length=141, Percent_Identity=32.6241134751773, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=252, Percent_Identity=36.9047619047619, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6320478, Length=264, Percent_Identity=30.6818181818182, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324614, Length=262, Percent_Identity=33.587786259542, Blast_Score=105, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=40.3846153846154, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=40.3846153846154, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=40.3846153846154, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI221330176, Length=217, Percent_Identity=28.110599078341, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_GLOVI (Q7NG34)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926285.1
- HSSP:   Q9SID3
- ProteinModelPortal:   Q7NG34
- GeneID:   2601742
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3339
- NMPDR:   fig|251221.1.peg.3339
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- BioCyc:   GVIO251221:GLL3339-MONOMER
- BRENDA:   3.1.2.6
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28123; Mature: 28123

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVHRLNALKDNYVFVLEDEAARTAAVVDPAEARPVLEALVRLGLKLVAIFNTHHHHDHV
CCCCHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GGNRELLEAYPGIAVYASRRDRGRIPGQTVELEDGDTVAFGCERARVIFVPGHTHGHIAY
CCCHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCEEEEECCCEEEEEECCCCCCEEEE
HFAGCGHLFCGDTLFAGGCGRLFEGTARQMQHSLGRLRELPGETQVWCAHEYTLGNLRFA
EEECCCCEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEE
HTLEPDNAELAERLRTVEVLRARKIATVPSTMAAERATNPFLRWESRQLQRAAGAIEPEE
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
VFAHLRALKDRF
HHHHHHHHHHCC
>Mature Secondary Structure
MLVHRLNALKDNYVFVLEDEAARTAAVVDPAEARPVLEALVRLGLKLVAIFNTHHHHDHV
CCCCHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GGNRELLEAYPGIAVYASRRDRGRIPGQTVELEDGDTVAFGCERARVIFVPGHTHGHIAY
CCCHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCEEEEECCCEEEEEECCCCCCEEEE
HFAGCGHLFCGDTLFAGGCGRLFEGTARQMQHSLGRLRELPGETQVWCAHEYTLGNLRFA
EEECCCCEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEE
HTLEPDNAELAERLRTVEVLRARKIATVPSTMAAERATNPFLRWESRQLQRAAGAIEPEE
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
VFAHLRALKDRF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292