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The map label for this gene is miaA

Identifier: 37522817

GI number: 37522817

Start: 3452713

End: 3453621

Strand: Reverse

Name: miaA

Synonym: gll3248

Alternate gene names: 37522817

Gene position: 3453621-3452713 (Counterclockwise)

Preceding gene: 37522818

Following gene: 37522816

Centisome position: 74.13

GC content: 65.02

Gene sequence:

>909_bases
GTGAAAAAAATCGTCGTCGTCTGCGGCCCCACCGCCGCCGGCAAAAGCCAACTGGGAATGCATCTGGCCCAGCACCTGGG
GGTACCGGTGCTGAGCGCCGATTCCCGGCAGGTCTACCGCGAGTTCGACATCGGCACTGCTAAACCGACCCGTGAAGAAC
AACGCCGCGTCGAGCACTGCCTCATCGATGTCGCCTGGCCCACGGAACATTTCAACGTGGCGCGCTACCGGGAACTGGCC
GATGCGGAGATTGACCGGCTGACCCGGCAAGGCAAACCCGCCCTACTGGTGGGAGGCAGTGGTTTGTACCTGCGCGCGGT
ATCGGGCGGACTCGAACCGCCCGCCGTCCCCCCCGACCCGGCTTTGCGCGCCCGCCTGGCGGTTGAGCGGTTGGACGCAC
TTTACCAGCGACTTCAACAGCTCGATCCCGAATCCGCGGGGCGCATCCACCCCAACGACCAGGTGCGCATCGAGCGCGCC
CTCGAAGTGTGCCTGACAACCGGCCAACCGCTGAGTGCCCAGCGCCGCCTGCGGGCGCGAGATTTTTCGCTCCTCGCCCT
GGGGGTGGGCAGCGGCCGGGAAGCGCTGGTGCGCCGCATCGAGCAGCGCACCCACCGGATGATCGAAGCGGGTTGGCTGG
AGGAAGTCGAGTATCTGCGCGCCAAATACGGCCCCGATTTGCCGCTTCTGAGCACCCTCGGTTACGCCGAGTTGGGGGCG
TACCTGGAGGAGCGATGGGATCTGGCGGAGGCGCTCCAGCAGATCGTCGTGCATACCCGCCAGTTCGCCAAGCGCCAGAT
GACCTGGTTTCGCGCCGAGCCCGATGTTCATTGGCTCGATGAATCGGCAGGACGGCAGACACTTGAACAACAGAGTATCG
AACAGGTTGAGCGCTTTCTTGCCGGTTGA

Upstream 100 bases:

>100_bases
CGATTTTTACCCCCAGGGAACTGGCTGCCTTCAACGAGCGAATGGGTCCCCTGCAGGCGGTGGAACGTTTTTTTAAAGGA
CCGAGCTTTTTTGACAATTG

Downstream 100 bases:

>100_bases
ACCCGAGCGTTTGCCGCCGAAGCCATCCCTGGGAAAGAGGTCGGCAGGTACAACGAAGGTCACTATGAAAGTAAACAGAT
GTCTGCGACCTGGAGGCAAA

Product: tRNA delta-2-isopentenylpyrophosphate

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKKIVVVCGPTAAGKSQLGMHLAQHLGVPVLSADSRQVYREFDIGTAKPTREEQRRVEHCLIDVAWPTEHFNVARYRELA
DAEIDRLTRQGKPALLVGGSGLYLRAVSGGLEPPAVPPDPALRARLAVERLDALYQRLQQLDPESAGRIHPNDQVRIERA
LEVCLTTGQPLSAQRRLRARDFSLLALGVGSGREALVRRIEQRTHRMIEAGWLEEVEYLRAKYGPDLPLLSTLGYAELGA
YLEERWDLAEALQQIVVHTRQFAKRQMTWFRAEPDVHWLDESAGRQTLEQQSIEQVERFLAG

Sequences:

>Translated_302_residues
MKKIVVVCGPTAAGKSQLGMHLAQHLGVPVLSADSRQVYREFDIGTAKPTREEQRRVEHCLIDVAWPTEHFNVARYRELA
DAEIDRLTRQGKPALLVGGSGLYLRAVSGGLEPPAVPPDPALRARLAVERLDALYQRLQQLDPESAGRIHPNDQVRIERA
LEVCLTTGQPLSAQRRLRARDFSLLALGVGSGREALVRRIEQRTHRMIEAGWLEEVEYLRAKYGPDLPLLSTLGYAELGA
YLEERWDLAEALQQIVVHTRQFAKRQMTWFRAEPDVHWLDESAGRQTLEQQSIEQVERFLAG
>Mature_302_residues
MKKIVVVCGPTAAGKSQLGMHLAQHLGVPVLSADSRQVYREFDIGTAKPTREEQRRVEHCLIDVAWPTEHFNVARYRELA
DAEIDRLTRQGKPALLVGGSGLYLRAVSGGLEPPAVPPDPALRARLAVERLDALYQRLQQLDPESAGRIHPNDQVRIERA
LEVCLTTGQPLSAQRRLRARDFSLLALGVGSGREALVRRIEQRTHRMIEAGWLEEVEYLRAKYGPDLPLLSTLGYAELGA
YLEERWDLAEALQQIVVHTRQFAKRQMTWFRAEPDVHWLDESAGRQTLEQQSIEQVERFLAG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=352, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1790613, Length=312, Percent_Identity=38.1410256410256, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=29.96632996633, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24649830, Length=348, Percent_Identity=29.8850574712644, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_GLOVI (Q7MBC1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926194.1
- ProteinModelPortal:   Q7MBC1
- SMR:   Q7MBC1
- GeneID:   2601649
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3248
- NMPDR:   fig|251221.1.peg.3248
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- BioCyc:   GVIO251221:GLL3248-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34076; Mature: 34076

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVVVCGPTAAGKSQLGMHLAQHLGVPVLSADSRQVYREFDIGTAKPTREEQRRVEHC
CCEEEEEECCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHH
LIDVAWPTEHFNVARYRELADAEIDRLTRQGKPALLVGGSGLYLRAVSGGLEPPAVPPDP
HEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCCCH
ALRARLAVERLDALYQRLQQLDPESAGRIHPNDQVRIERALEVCLTTGQPLSAQRRLRAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHC
DFSLLALGVGSGREALVRRIEQRTHRMIEAGWLEEVEYLRAKYGPDLPLLSTLGYAELGA
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHH
YLEERWDLAEALQQIVVHTRQFAKRQMTWFRAEPDVHWLDESAGRQTLEQQSIEQVERFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AG
CC
>Mature Secondary Structure
MKKIVVVCGPTAAGKSQLGMHLAQHLGVPVLSADSRQVYREFDIGTAKPTREEQRRVEHC
CCEEEEEECCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHH
LIDVAWPTEHFNVARYRELADAEIDRLTRQGKPALLVGGSGLYLRAVSGGLEPPAVPPDP
HEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCCCH
ALRARLAVERLDALYQRLQQLDPESAGRIHPNDQVRIERALEVCLTTGQPLSAQRRLRAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHC
DFSLLALGVGSGREALVRRIEQRTHRMIEAGWLEEVEYLRAKYGPDLPLLSTLGYAELGA
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHH
YLEERWDLAEALQQIVVHTRQFAKRQMTWFRAEPDVHWLDESAGRQTLEQQSIEQVERFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292