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The map label for this gene is prfC

Identifier: 37522728

GI number: 37522728

Start: 3360702

End: 3362297

Strand: Direct

Name: prfC

Synonym: glr3159

Alternate gene names: 37522728

Gene position: 3360702-3362297 (Clockwise)

Preceding gene: 37522725

Following gene: 37522729

Centisome position: 72.13

GC content: 61.34

Gene sequence:

>1596_bases
ATGAGCAATCCCACCCTCGCCGCCGAAGTCGGCAGACGCCGCACCTTCGCCATTATCTCGCACCCCGACGCCGGCAAGAC
GACGCTCACCGAGAAGCTCCTGCTCTACGGAGGGGCGATCGACATGGCCGGTTCGGTGCGCGCCCGGCGCAACCAGCGCC
ACGCCACCTCCGACTGGATGGCCATGGAACAGCAGCGCGGCATCTCGATCACCTCGACGGTGCTGCAGTTTGTCTACCGC
GACTGCCAGATCAATTTGCTGGATACCCCGGGCCACCAGGATTTTTCGGAGGATACCTACCGGACCCTCGCCGCCGCCGA
CAACGCCGCGATGCTCATCGACGCCGCCAAAGGCATCGAAGCCCAGACGCGCAAGCTCTTCGACGTCTGCCGGATGCGCG
GCATTCCGATTTTTACGTTTATCAACAAACTGGATCGGCCGGGGCGCGAGCCGCTCGAACTGTTGGACGAGATCGAAAAA
GTGCTGGGCATCGACGTCTACCCAGTCAACTGGCCGATCGGCATGGGCGATACCTTCCGGGGGGTCTTCGATCGCCTGGA
GCATACGGTTCATCTGTTCGACCGCACGACCGGGGGCAAGAAGATGGCGCCGGTGACGGTGGGGGCGATTGGGGACGAGC
GCATGCGCGTGCTGATGGACGAAGGCACCTACGCCCAGATTGTCGAAGAGATCGAGCTGTTGGATGGGGTTGGGGTACCT
TTCGACATCGAGCGTGTAGCGCAGGGCAAACTCACCCCGGTCTTCTTCGGCAGTGCCGCCAACAACTTTGGGGTGCAGCT
GTTTCTCGATGCGTTTATTCGCTTTGCCTCGCGGCCGGGGACGCGATCGGCCAATACCGGCGTGATTTCGCCGGTGGCTG
AGGATTTTTCGGGTTTCGTCTTCAAGATCCAGGCCAACATGGACCCGCAGCACCGCGACCGGGTCGCGTTTATTCGCGTT
TGCTCGGGCAAGTTTGAAAAGGACATGACCGTCCACCACACCCGTTCGGGCAAAAAGGTGCGCCTGTCGCGCTCGCTCAA
ACTCTTCGGCCAGGAGCGCGAGACGGTCGAAGAAGCCTACGCGGGCGACATCGTCGGGGTCATTAACCCCGGCACCTTCG
CCATCGGCGACACGATCTGTCTGGGCAAACCGCTGGCTTTTGAAGGTATCCCGCTTTTTCCGCCCGAGCACTTCGCCACC
CTCCGAAACCCGAACCCTTCCAAGTACAAGCAGTTTCTCAAGGGCGTCACCCAATTGCGCGAGGAAGGCGCGGTGCAGGT
ACTCTTTCACCAGGACGAAGCGAAGCGCGATCCGATTCTGGCCGCGGTGGGCCAGCTGCAGTTCGACGTCGTGCGTTTTC
GCCTCGAAAGCGAGTACCACGTCGAGACGATCCTCGAACCGCTGCCCTGGACACTGGCGCGCTGGATCACCGCCAAGCAG
CTGGAGGATCTCGAAACGATCGACTGGTACTTCGACAGTCTGGGCCTCAAGGACCACGAGGGTCGTCTGGTGATCCTCTT
CAAGACCCCCTGGGGCTTTCAGCAGATGAGCGAGCGCAATCCGCATCTGCAATTTCACGAAATAGCGCCGCTCTAA

Upstream 100 bases:

>100_bases
TCTAATCTTAGAATTAATCAAGTTCGGGACGATGTCAACTTGAGCCCCCCGACCGCAAGACGTTAGGCTAGGGGCAGTAA
TTGCCCGAAATTCTGCCCCC

Downstream 100 bases:

>100_bases
ATCGCCTTTTTGTAGGCAGCTGCACAATGTAGCACTGCACAATATGCTGTTTTCTTTATTAAGGAATTCCAGCTTGTGAT
GGAGCGCTCGGAGCCACTAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSNPTLAAEVGRRRTFAIISHPDAGKTTLTEKLLLYGGAIDMAGSVRARRNQRHATSDWMAMEQQRGISITSTVLQFVYR
DCQINLLDTPGHQDFSEDTYRTLAAADNAAMLIDAAKGIEAQTRKLFDVCRMRGIPIFTFINKLDRPGREPLELLDEIEK
VLGIDVYPVNWPIGMGDTFRGVFDRLEHTVHLFDRTTGGKKMAPVTVGAIGDERMRVLMDEGTYAQIVEEIELLDGVGVP
FDIERVAQGKLTPVFFGSAANNFGVQLFLDAFIRFASRPGTRSANTGVISPVAEDFSGFVFKIQANMDPQHRDRVAFIRV
CSGKFEKDMTVHHTRSGKKVRLSRSLKLFGQERETVEEAYAGDIVGVINPGTFAIGDTICLGKPLAFEGIPLFPPEHFAT
LRNPNPSKYKQFLKGVTQLREEGAVQVLFHQDEAKRDPILAAVGQLQFDVVRFRLESEYHVETILEPLPWTLARWITAKQ
LEDLETIDWYFDSLGLKDHEGRLVILFKTPWGFQQMSERNPHLQFHEIAPL

Sequences:

>Translated_531_residues
MSNPTLAAEVGRRRTFAIISHPDAGKTTLTEKLLLYGGAIDMAGSVRARRNQRHATSDWMAMEQQRGISITSTVLQFVYR
DCQINLLDTPGHQDFSEDTYRTLAAADNAAMLIDAAKGIEAQTRKLFDVCRMRGIPIFTFINKLDRPGREPLELLDEIEK
VLGIDVYPVNWPIGMGDTFRGVFDRLEHTVHLFDRTTGGKKMAPVTVGAIGDERMRVLMDEGTYAQIVEEIELLDGVGVP
FDIERVAQGKLTPVFFGSAANNFGVQLFLDAFIRFASRPGTRSANTGVISPVAEDFSGFVFKIQANMDPQHRDRVAFIRV
CSGKFEKDMTVHHTRSGKKVRLSRSLKLFGQERETVEEAYAGDIVGVINPGTFAIGDTICLGKPLAFEGIPLFPPEHFAT
LRNPNPSKYKQFLKGVTQLREEGAVQVLFHQDEAKRDPILAAVGQLQFDVVRFRLESEYHVETILEPLPWTLARWITAKQ
LEDLETIDWYFDSLGLKDHEGRLVILFKTPWGFQQMSERNPHLQFHEIAPL
>Mature_530_residues
SNPTLAAEVGRRRTFAIISHPDAGKTTLTEKLLLYGGAIDMAGSVRARRNQRHATSDWMAMEQQRGISITSTVLQFVYRD
CQINLLDTPGHQDFSEDTYRTLAAADNAAMLIDAAKGIEAQTRKLFDVCRMRGIPIFTFINKLDRPGREPLELLDEIEKV
LGIDVYPVNWPIGMGDTFRGVFDRLEHTVHLFDRTTGGKKMAPVTVGAIGDERMRVLMDEGTYAQIVEEIELLDGVGVPF
DIERVAQGKLTPVFFGSAANNFGVQLFLDAFIRFASRPGTRSANTGVISPVAEDFSGFVFKIQANMDPQHRDRVAFIRVC
SGKFEKDMTVHHTRSGKKVRLSRSLKLFGQERETVEEAYAGDIVGVINPGTFAIGDTICLGKPLAFEGIPLFPPEHFATL
RNPNPSKYKQFLKGVTQLREEGAVQVLFHQDEAKRDPILAAVGQLQFDVVRFRLESEYHVETILEPLPWTLARWITAKQL
EDLETIDWYFDSLGLKDHEGRLVILFKTPWGFQQMSERNPHLQFHEIAPL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=29.9196787148594, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI19923640, Length=541, Percent_Identity=26.4325323475046, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=28.3544303797468, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25306287, Length=526, Percent_Identity=24.7148288973384, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI157426893, Length=156, Percent_Identity=33.974358974359, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.2013422818792, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790835, Length=519, Percent_Identity=48.5549132947977, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=29.1581108829569, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788922, Length=179, Percent_Identity=32.4022346368715, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=35.1724137931034, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=479, Percent_Identity=28.39248434238, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17556745, Length=177, Percent_Identity=32.7683615819209, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=37.037037037037, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=493, Percent_Identity=27.5862068965517, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=571, Percent_Identity=25.569176882662, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=33.7748344370861, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=31.4465408805031, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=31.4465408805031, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582462, Length=476, Percent_Identity=28.9915966386555, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI221458488, Length=518, Percent_Identity=26.0617760617761, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI78706572, Length=188, Percent_Identity=34.0425531914894, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574573, Length=172, Percent_Identity=31.3953488372093, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_GLOVI (Q7NGL0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926105.1
- ProteinModelPortal:   Q7NGL0
- GeneID:   2601560
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr3159
- NMPDR:   fig|251221.1.peg.3159
- HOGENOM:   HBG285678
- OMA:   HERTVEP
- ProtClustDB:   PRK00741
- BioCyc:   GVIO251221:GLR3159-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59737; Mature: 59606

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPTLAAEVGRRRTFAIISHPDAGKTTLTEKLLLYGGAIDMAGSVRARRNQRHATSDWM
CCCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHH
AMEQQRGISITSTVLQFVYRDCQINLLDTPGHQDFSEDTYRTLAAADNAAMLIDAAKGIE
HHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCH
AQTRKLFDVCRMRGIPIFTFINKLDRPGREPLELLDEIEKVLGIDVYPVNWPIGMGDTFR
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHH
GVFDRLEHTVHLFDRTTGGKKMAPVTVGAIGDERMRVLMDEGTYAQIVEEIELLDGVGVP
HHHHHHHHHEEEEECCCCCCEECCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
FDIERVAQGKLTPVFFGSAANNFGVQLFLDAFIRFASRPGTRSANTGVISPVAEDFSGFV
CCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEE
FKIQANMDPQHRDRVAFIRVCSGKFEKDMTVHHTRSGKKVRLSRSLKLFGQERETVEEAY
EEEECCCCCCHHCCEEEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHCCHHHHHHHHH
AGDIVGVINPGTFAIGDTICLGKPLAFEGIPLFPPEHFATLRNPNPSKYKQFLKGVTQLR
CCCEEEEECCCCEEECCEEECCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
EEGAVQVLFHQDEAKRDPILAAVGQLQFDVVRFRLESEYHVETILEPLPWTLARWITAKQ
HCCCEEEEEECCCCCCCCHHHHHHHHHEEEHHEECCCCCCHHHHHCCCCHHHHHHHHHHH
LEDLETIDWYFDSLGLKDHEGRLVILFKTPWGFQQMSERNPHLQFHEIAPL
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure 
SNPTLAAEVGRRRTFAIISHPDAGKTTLTEKLLLYGGAIDMAGSVRARRNQRHATSDWM
CCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHH
AMEQQRGISITSTVLQFVYRDCQINLLDTPGHQDFSEDTYRTLAAADNAAMLIDAAKGIE
HHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCH
AQTRKLFDVCRMRGIPIFTFINKLDRPGREPLELLDEIEKVLGIDVYPVNWPIGMGDTFR
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHH
GVFDRLEHTVHLFDRTTGGKKMAPVTVGAIGDERMRVLMDEGTYAQIVEEIELLDGVGVP
HHHHHHHHHEEEEECCCCCCEECCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
FDIERVAQGKLTPVFFGSAANNFGVQLFLDAFIRFASRPGTRSANTGVISPVAEDFSGFV
CCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEE
FKIQANMDPQHRDRVAFIRVCSGKFEKDMTVHHTRSGKKVRLSRSLKLFGQERETVEEAY
EEEECCCCCCHHCCEEEEEECCCCCCCCCEEEECCCCCEEEEEHHHHHHCCHHHHHHHHH
AGDIVGVINPGTFAIGDTICLGKPLAFEGIPLFPPEHFATLRNPNPSKYKQFLKGVTQLR
CCCEEEEECCCCEEECCEEECCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
EEGAVQVLFHQDEAKRDPILAAVGQLQFDVVRFRLESEYHVETILEPLPWTLARWITAKQ
HCCCEEEEEECCCCCCCCHHHHHHHHHEEEHHEECCCCCCHHHHHCCCCHHHHHHHHHHH
LEDLETIDWYFDSLGLKDHEGRLVILFKTPWGFQQMSERNPHLQFHEIAPL
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292