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The map label for this gene is ykuU [H]

Identifier: 37522727

GI number: 37522727

Start: 3360006

End: 3360560

Strand: Reverse

Name: ykuU [H]

Synonym: gll3158

Alternate gene names: 37522727

Gene position: 3360560-3360006 (Counterclockwise)

Preceding gene: 37522731

Following gene: 37522726

Centisome position: 72.13

GC content: 58.2

Gene sequence:

>555_bases
ATGACCGCATCCGTTGGACTTCCGGCTCCGAGCTTCACGCTGAAAAGCACCAAGGGTGCCACCAGCCCCAAAAATCTTGG
TGAGCCCATCGCGCTCACCGACTACCGGGGCAAGTGGTTGATCTTCTTTTTCTATCCGCTCGATTTTACGTTTGTATGCC
CGACTGAAATTCTTGCCCTCTCCGATCGTTACGAAGAGTTTCAAGATCTCGACACCGAAGTGCTCGGTTGCTCCTGCGAC
AGCGTCTTTAGCCACTGGGCCTGGATCAGCACCCCGCGCGAGAAAAACGGCATTGCCGGACTGAAGTTCCCGCTGGTCGC
CGATTTCACCAAAGATGTCGCCGAAGCCTACGGCGTGCTCAACGAAAAAGAAGGCTACTCCCAGCGCGGGCTGTTTATCA
TCGATCCCGACGGCATCCTCAAGTACGCGACGATCACCGACGACAACGTCGGCCGCTCGGTCGAGGAGACTATCCGCGTG
CTGCAGGCGCTGCAAAACGGCGGCCTGTGCCCGGGCGACTGGAAGCCCGGCCAGAAAGTCCTCGAAGTCGTCTAG

Upstream 100 bases:

>100_bases
CTTGCGGTCGGGGGGCTCAAGTTGACATCGTCCCGAACTTGATTAATTCTAAGATTAGACTTTGTACGCATTGGCCATTC
CCAGAGGAGACCCGCCCACC

Downstream 100 bases:

>100_bases
CCATGGCACTGCGCATGCGCGCTCCCCTGCCCTCTTTGGCGGGGGTGCCCACCTGGATTAACGGCGGCGAACCGAAGCCC
GAGGATTACCAGGGCAAGGT

Product: peroxiredoxin

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MTASVGLPAPSFTLKSTKGATSPKNLGEPIALTDYRGKWLIFFFYPLDFTFVCPTEILALSDRYEEFQDLDTEVLGCSCD
SVFSHWAWISTPREKNGIAGLKFPLVADFTKDVAEAYGVLNEKEGYSQRGLFIIDPDGILKYATITDDNVGRSVEETIRV
LQALQNGGLCPGDWKPGQKVLEVV

Sequences:

>Translated_184_residues
MTASVGLPAPSFTLKSTKGATSPKNLGEPIALTDYRGKWLIFFFYPLDFTFVCPTEILALSDRYEEFQDLDTEVLGCSCD
SVFSHWAWISTPREKNGIAGLKFPLVADFTKDVAEAYGVLNEKEGYSQRGLFIIDPDGILKYATITDDNVGRSVEETIRV
LQALQNGGLCPGDWKPGQKVLEVV
>Mature_183_residues
TASVGLPAPSFTLKSTKGATSPKNLGEPIALTDYRGKWLIFFFYPLDFTFVCPTEILALSDRYEEFQDLDTEVLGCSCDS
VFSHWAWISTPREKNGIAGLKFPLVADFTKDVAEAYGVLNEKEGYSQRGLFIIDPDGILKYATITDDNVGRSVEETIRVL
QALQNGGLCPGDWKPGQKVLEVV

Specific function: Directly Reduces Organic Hydroperoxides In Its Reduced Dithiol Form. [C]

COG id: COG0450

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI4505591, Length=185, Percent_Identity=50.8108108108108, Blast_Score=192, Evalue=1e-49,
Organism=Homo sapiens, GI32455266, Length=185, Percent_Identity=50.8108108108108, Blast_Score=192, Evalue=1e-49,
Organism=Homo sapiens, GI32455264, Length=185, Percent_Identity=50.8108108108108, Blast_Score=192, Evalue=1e-49,
Organism=Homo sapiens, GI5453549, Length=156, Percent_Identity=54.4871794871795, Blast_Score=188, Evalue=2e-48,
Organism=Homo sapiens, GI32189392, Length=185, Percent_Identity=48.6486486486487, Blast_Score=185, Evalue=2e-47,
Organism=Homo sapiens, GI5802974, Length=151, Percent_Identity=51.6556291390728, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI32483377, Length=151, Percent_Identity=51.6556291390728, Blast_Score=178, Evalue=2e-45,
Organism=Homo sapiens, GI33188454, Length=87, Percent_Identity=50.5747126436782, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI4758638, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786822, Length=162, Percent_Identity=38.2716049382716, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554494, Length=170, Percent_Identity=51.1764705882353, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI193204376, Length=186, Percent_Identity=48.3870967741936, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32565831, Length=186, Percent_Identity=48.3870967741936, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25153706, Length=192, Percent_Identity=27.0833333333333, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320661, Length=186, Percent_Identity=42.4731182795699, Blast_Score=151, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323613, Length=186, Percent_Identity=39.7849462365591, Blast_Score=147, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319407, Length=186, Percent_Identity=30.1075268817204, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17738015, Length=177, Percent_Identity=50.8474576271186, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI17157991, Length=178, Percent_Identity=50.561797752809, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24641739, Length=178, Percent_Identity=50.561797752809, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24656348, Length=179, Percent_Identity=47.4860335195531, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI17864676, Length=179, Percent_Identity=47.4860335195531, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21357347, Length=178, Percent_Identity=44.3820224719101, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17975518, Length=191, Percent_Identity=31.413612565445, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652434, Length=191, Percent_Identity=31.413612565445, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24652436, Length=191, Percent_Identity=31.413612565445, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24581278, Length=189, Percent_Identity=27.5132275132275, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2250 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 6040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Stationary-Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000866
- InterPro:   IPR019479
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10417 1-cysPrx_C; PF00578 AhpC-TSA [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 20322; Mature: 20191

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVGLPAPSFTLKSTKGATSPKNLGEPIALTDYRGKWLIFFFYPLDFTFVCPTEILAL
CCCCCCCCCCCEEEECCCCCCCCHHCCCCEEEEECCCCEEEEEEECCCEEEECHHHHHHH
SDRYEEFQDLDTEVLGCSCDSVFSHWAWISTPREKNGIAGLKFPLVADFTKDVAEAYGVL
HHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
NEKEGYSQRGLFIIDPDGILKYATITDDNVGRSVEETIRVLQALQNGGLCPGDWKPGQKV
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
LEVV
HHCC
>Mature Secondary Structure 
TASVGLPAPSFTLKSTKGATSPKNLGEPIALTDYRGKWLIFFFYPLDFTFVCPTEILAL
CCCCCCCCCCEEEECCCCCCCCHHCCCCEEEEECCCCEEEEEEECCCEEEECHHHHHHH
SDRYEEFQDLDTEVLGCSCDSVFSHWAWISTPREKNGIAGLKFPLVADFTKDVAEAYGVL
HHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
NEKEGYSQRGLFIIDPDGILKYATITDDNVGRSVEETIRVLQALQNGGLCPGDWKPGQKV
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
LEVV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]