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The map label for this gene is pnp

Identifier: 37522718

GI number: 37522718

Start: 3352676

End: 3354817

Strand: Reverse

Name: pnp

Synonym: gll3149

Alternate gene names: 37522718

Gene position: 3354817-3352676 (Counterclockwise)

Preceding gene: 37522724

Following gene: 37522716

Centisome position: 72.01

GC content: 62.51

Gene sequence:

>2142_bases
ATGATCGAGTACAGAAAGTCTGTCACAGTCCACGGACGCGAGATCGGGTTGAGTACCGGCGTCCTGGCGCAGTTGGCGGG
CGGTTCGGTGTTGGTGCAGTCGGGGGAGACTGCGGTATTGGTGACGGCCACGATGGCCAGCAAACCCCGGGAGGGGATCG
ACTTCTTTCCATTGCTGGTGGATTACGAAGAACGCCTGTACGCGGCCGGACGCATTCCAGGCAGTTTCGTGCGCCGCGAA
GGCCGCCCGCCCGAAAAAGCAATTCTTGCCGCCCGCCTGATTGACCGGCCGCTGCGGCCCCTCTTTCCAAAGGGCTTCAT
CAACGACGTGCAGATCGTGGCGACGGTGCTTGCCCTCGATATGAACGTGCCGCCGGATATCCTTGCCATCCTCGGCGCCT
CGACGGCGACCATGCTCGCGGGCATTCCCTTCGACGGACCGGTGGCGGCTACCCGCGTCGGTTTGATGGGCGACGATTTC
ATCATCAACCCCACCTACAAAGAGATCGAAGAGGGCGAACTCGACTTAGTAGTGGCGGGCACCAAAAACGGGATCATGAT
GGTCGAGGCCGGGGCCAACTGCCTGCCCGAGGAGGACATCATCGATGCCATCGACTACGGCTACGAGGCGGTCCAGGCGA
TGCTCACCGCCCAGGAGGAGTTCATCCGCGACCTGAAGATTGAGCCGATGGCCTTCGTCGCCCCCGCCAACGACCCGGTG
CTGGTTTCCTTTGTCGAAGAGAAAGCTTCCGAGGCGGTCGCCGAGGTGCTGCGCAGCTTCGAGCTGAGCAAAGACGAGCG
CGACGCCAAGCTCGACGCTATCGAGGCAGAACTGGGGACCACCTTTGCAGGTCTGGGCGAAGAAGACCCCCTGCGCGAGA
AAGCGGCCGCCAATGCCAGCAAGTTGCCGGCGCTCTTCAAATCCACCACCAAAAAGCTGATGCGCAAGCAGATCCTGGTG
GAGGGCAAGCGTGTCGACGGCCGTTCCCTCGACGAGGTGCGTCCCATCTCAAGTGCCGTGCGGGTGATCCCGCGGGTGCA
CGGTTGTGGCCTGTTCACCCGGGGCACCACCCAGGTGCTCTCGGTGGTGACCCTGGGCACCGCCTCCGACGCCCAGGAAC
TCGACGATCTGCACCCGGACGATTCCAAGCGCTACATGCACCACTACAACTTTCCGGGTTTTTCGGTGGGCGAAGTGAAG
CCCCTGCGCGGCGTCGGCCGCCGCGAAGTGGGCCACGGCGCCCTCGCCGAGCGCGCCATCGTGCCGATCCTGCCCGAGCA
CGAGCAGTTTCCTTACGTGATCCGCGTCGTCTCCGAGGTGCTCTCCTCCAACGGTTCAACCTCGATGGGCTCGGTGTGCG
CCTCGACCCTCGCCCTGATGGATGCCGGGGTGCCCATCCGCGAACCGGTCTCCGGGGTGGCGATGGGTCTAATTAAAGAA
GGCGACGAAGTGCGCATCCTCACCGACATCCAGGGCATCGAGGATTTTCTGGGGGACATGGACTTCAAGGTGGCGGGCAC
CCGCGACGGGATCACCGCGCTGCAGATGGACATTAAAATCCAGGGGATCACCCTGCAGATCCTCGAAGCCGCCATCCAGC
AGTCCAAGGCGGGCCGACTGCACATCCTGGAGAAGATGCTGATCGCCATCGACAAACCGCGGGTCGAACTGTCGCCCTAC
GCGCCGCGCCTGCTCACCCTCAAGATCCCGGTCGACATGATCGGTCTGGTGATTGGTCCCGGCGGTAAGACGATCAAGCG
CATCGTCGAGGAGACCGGCGCCAAGGTCGATATCGAAGACGACGGCACCGTGGTGGTTTCTTCGATAGACGGGGCGAAGG
CTCTGGCTGCCAAGCAGATCATCGAGGGGATGACCCGCACCGTCGAAGTCGACAAAGTTTATCTGGGTACGGTCACCCGG
ATTATCCCCAACCTGGGGGCTTTCGTCGAAGTCATGCCCGGCAAAGAGGGCTTAGTGCATATCTCACAGCTTGCTGAGCA
CCGGGTGCGCAAAGTCGAAGACGAACTGAACGTCGGCGACGAAGTCGTCGTGAAAGTCAAAGAGATCGATCAAAAAGGCC
GCATCAACCTCACCCGCCGGGGAATCCACCCGAGCGAGGTCGAGGAGGCGCGGGGGCAGTAG

Upstream 100 bases:

>100_bases
TCATGCCCCGGCCGGCGGCGGCTCGCTTGCGATCCCGGCCCGTCCACTACCGCCGGTCCCATTTGTTAGACTCTAACGAG
TACAAAAGACAGAGAAGAAT

Downstream 100 bases:

>100_bases
CCCTCTGGATCCCCCCCTGCCCCCCCTTTCCAAGGGGGGCAGCGCAGCGGGGGGGATCCATCCCTACAATCCCGGTCAGG
CGCCTGGCTTCGGCAAGGGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MIEYRKSVTVHGREIGLSTGVLAQLAGGSVLVQSGETAVLVTATMASKPREGIDFFPLLVDYEERLYAAGRIPGSFVRRE
GRPPEKAILAARLIDRPLRPLFPKGFINDVQIVATVLALDMNVPPDILAILGASTATMLAGIPFDGPVAATRVGLMGDDF
IINPTYKEIEEGELDLVVAGTKNGIMMVEAGANCLPEEDIIDAIDYGYEAVQAMLTAQEEFIRDLKIEPMAFVAPANDPV
LVSFVEEKASEAVAEVLRSFELSKDERDAKLDAIEAELGTTFAGLGEEDPLREKAAANASKLPALFKSTTKKLMRKQILV
EGKRVDGRSLDEVRPISSAVRVIPRVHGCGLFTRGTTQVLSVVTLGTASDAQELDDLHPDDSKRYMHHYNFPGFSVGEVK
PLRGVGRREVGHGALAERAIVPILPEHEQFPYVIRVVSEVLSSNGSTSMGSVCASTLALMDAGVPIREPVSGVAMGLIKE
GDEVRILTDIQGIEDFLGDMDFKVAGTRDGITALQMDIKIQGITLQILEAAIQQSKAGRLHILEKMLIAIDKPRVELSPY
APRLLTLKIPVDMIGLVIGPGGKTIKRIVEETGAKVDIEDDGTVVVSSIDGAKALAAKQIIEGMTRTVEVDKVYLGTVTR
IIPNLGAFVEVMPGKEGLVHISQLAEHRVRKVEDELNVGDEVVVKVKEIDQKGRINLTRRGIHPSEVEEARGQ

Sequences:

>Translated_713_residues
MIEYRKSVTVHGREIGLSTGVLAQLAGGSVLVQSGETAVLVTATMASKPREGIDFFPLLVDYEERLYAAGRIPGSFVRRE
GRPPEKAILAARLIDRPLRPLFPKGFINDVQIVATVLALDMNVPPDILAILGASTATMLAGIPFDGPVAATRVGLMGDDF
IINPTYKEIEEGELDLVVAGTKNGIMMVEAGANCLPEEDIIDAIDYGYEAVQAMLTAQEEFIRDLKIEPMAFVAPANDPV
LVSFVEEKASEAVAEVLRSFELSKDERDAKLDAIEAELGTTFAGLGEEDPLREKAAANASKLPALFKSTTKKLMRKQILV
EGKRVDGRSLDEVRPISSAVRVIPRVHGCGLFTRGTTQVLSVVTLGTASDAQELDDLHPDDSKRYMHHYNFPGFSVGEVK
PLRGVGRREVGHGALAERAIVPILPEHEQFPYVIRVVSEVLSSNGSTSMGSVCASTLALMDAGVPIREPVSGVAMGLIKE
GDEVRILTDIQGIEDFLGDMDFKVAGTRDGITALQMDIKIQGITLQILEAAIQQSKAGRLHILEKMLIAIDKPRVELSPY
APRLLTLKIPVDMIGLVIGPGGKTIKRIVEETGAKVDIEDDGTVVVSSIDGAKALAAKQIIEGMTRTVEVDKVYLGTVTR
IIPNLGAFVEVMPGKEGLVHISQLAEHRVRKVEDELNVGDEVVVKVKEIDQKGRINLTRRGIHPSEVEEARGQ
>Mature_713_residues
MIEYRKSVTVHGREIGLSTGVLAQLAGGSVLVQSGETAVLVTATMASKPREGIDFFPLLVDYEERLYAAGRIPGSFVRRE
GRPPEKAILAARLIDRPLRPLFPKGFINDVQIVATVLALDMNVPPDILAILGASTATMLAGIPFDGPVAATRVGLMGDDF
IINPTYKEIEEGELDLVVAGTKNGIMMVEAGANCLPEEDIIDAIDYGYEAVQAMLTAQEEFIRDLKIEPMAFVAPANDPV
LVSFVEEKASEAVAEVLRSFELSKDERDAKLDAIEAELGTTFAGLGEEDPLREKAAANASKLPALFKSTTKKLMRKQILV
EGKRVDGRSLDEVRPISSAVRVIPRVHGCGLFTRGTTQVLSVVTLGTASDAQELDDLHPDDSKRYMHHYNFPGFSVGEVK
PLRGVGRREVGHGALAERAIVPILPEHEQFPYVIRVVSEVLSSNGSTSMGSVCASTLALMDAGVPIREPVSGVAMGLIKE
GDEVRILTDIQGIEDFLGDMDFKVAGTRDGITALQMDIKIQGITLQILEAAIQQSKAGRLHILEKMLIAIDKPRVELSPY
APRLLTLKIPVDMIGLVIGPGGKTIKRIVEETGAKVDIEDDGTVVVSSIDGAKALAAKQIIEGMTRTVEVDKVYLGTVTR
IIPNLGAFVEVMPGKEGLVHISQLAEHRVRKVEDELNVGDEVVVKVKEIDQKGRINLTRRGIHPSEVEEARGQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=719, Percent_Identity=38.6648122392211, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=49.0510948905109, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=34.9228611500701, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281362905, Length=714, Percent_Identity=38.655462184874, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=714, Percent_Identity=38.655462184874, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=714, Percent_Identity=38.655462184874, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=658, Percent_Identity=37.9939209726444, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_GLOVI (Q7NGM0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926095.1
- HSSP:   P05055
- ProteinModelPortal:   Q7NGM0
- GeneID:   2601550
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3149
- NMPDR:   fig|251221.1.peg.3149
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   GVIO251221:GLL3149-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77079; Mature: 77079

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEYRKSVTVHGREIGLSTGVLAQLAGGSVLVQSGETAVLVTATMASKPREGIDFFPLLV
CCCCCCCEEECCHHHCCCHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCHHHHHH
DYEERLYAAGRIPGSFVRREGRPPEKAILAARLIDRPLRPLFPKGFINDVQIVATVLALD
CHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
MNVPPDILAILGASTATMLAGIPFDGPVAATRVGLMGDDFIINPTYKEIEEGELDLVVAG
CCCCHHHHHHHCCCHHHHEECCCCCCCHHHHEECCCCCCEEECCCHHHHCCCCEEEEEEE
TKNGIMMVEAGANCLPEEDIIDAIDYGYEAVQAMLTAQEEFIRDLKIEPMAFVAPANDPV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCE
LVSFVEEKASEAVAEVLRSFELSKDERDAKLDAIEAELGTTFAGLGEEDPLREKAAANAS
EEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHH
KLPALFKSTTKKLMRKQILVEGKRVDGRSLDEVRPISSAVRVIPRVHGCGLFTRGTTQVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHH
SVVTLGTASDAQELDDLHPDDSKRYMHHYNFPGFSVGEVKPLRGVGRREVGHGALAERAI
HHHEECCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCCHHCCCCHHHHCCC
VPILPEHEQFPYVIRVVSEVLSSNGSTSMGSVCASTLALMDAGVPIREPVSGVAMGLIKE
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GDEVRILTDIQGIEDFLGDMDFKVAGTRDGITALQMDIKIQGITLQILEAAIQQSKAGRL
CCCEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCHH
HILEKMLIAIDKPRVELSPYAPRLLTLKIPVDMIGLVIGPGGKTIKRIVEETGAKVDIED
HHHHHHHHHHCCCCEEECCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHCCCEEEECC
DGTVVVSSIDGAKALAAKQIIEGMTRTVEVDKVYLGTVTRIIPNLGAFVEVMPGKEGLVH
CCEEEEEECCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
ISQLAEHRVRKVEDELNVGDEVVVKVKEIDQKGRINLTRRGIHPSEVEEARGQ
HHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCEEEEECCCCHHHHHHHCCC
>Mature Secondary Structure
MIEYRKSVTVHGREIGLSTGVLAQLAGGSVLVQSGETAVLVTATMASKPREGIDFFPLLV
CCCCCCCEEECCHHHCCCHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCHHHHHH
DYEERLYAAGRIPGSFVRREGRPPEKAILAARLIDRPLRPLFPKGFINDVQIVATVLALD
CHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
MNVPPDILAILGASTATMLAGIPFDGPVAATRVGLMGDDFIINPTYKEIEEGELDLVVAG
CCCCHHHHHHHCCCHHHHEECCCCCCCHHHHEECCCCCCEEECCCHHHHCCCCEEEEEEE
TKNGIMMVEAGANCLPEEDIIDAIDYGYEAVQAMLTAQEEFIRDLKIEPMAFVAPANDPV
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCE
LVSFVEEKASEAVAEVLRSFELSKDERDAKLDAIEAELGTTFAGLGEEDPLREKAAANAS
EEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHH
KLPALFKSTTKKLMRKQILVEGKRVDGRSLDEVRPISSAVRVIPRVHGCGLFTRGTTQVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHH
SVVTLGTASDAQELDDLHPDDSKRYMHHYNFPGFSVGEVKPLRGVGRREVGHGALAERAI
HHHEECCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCCHHCCCCHHHHCCC
VPILPEHEQFPYVIRVVSEVLSSNGSTSMGSVCASTLALMDAGVPIREPVSGVAMGLIKE
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GDEVRILTDIQGIEDFLGDMDFKVAGTRDGITALQMDIKIQGITLQILEAAIQQSKAGRL
CCCEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCHH
HILEKMLIAIDKPRVELSPYAPRLLTLKIPVDMIGLVIGPGGKTIKRIVEETGAKVDIED
HHHHHHHHHHCCCCEEECCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHCCCEEEECC
DGTVVVSSIDGAKALAAKQIIEGMTRTVEVDKVYLGTVTRIIPNLGAFVEVMPGKEGLVH
CCEEEEEECCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
ISQLAEHRVRKVEDELNVGDEVVVKVKEIDQKGRINLTRRGIHPSEVEEARGQ
HHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCEEEEECCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292