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The map label for this gene is pheS

Identifier: 37522697

GI number: 37522697

Start: 3331220

End: 3332236

Strand: Direct

Name: pheS

Synonym: gvip433

Alternate gene names: 37522697

Gene position: 3331220-3332236 (Clockwise)

Preceding gene: 37522694

Following gene: 37522698

Centisome position: 71.5

GC content: 64.7

Gene sequence:

>1017_bases
ATGCAGCAGCAACTCGACGCGCTCCAGCTCGAAGCCCTCAAAGCGATCCGCGAAGCGGCGGATCTGACCATCCTGGAGCG
CATCCGCGTCGACTTTTTGGGCAAAAAAGGCAAGCTTTCGGCGCTTCTGGGCGGCATGGCCAGGCTACCCGCCGAGGAGC
GGCCGGTCGTGGGCGCCCTGGTCAACCAGGTGAAAGCCCGGGTCGAGGAGGCGCTCGCTGCCCAGCAGACCCACCTGCAA
GACCAGCTTATCGAGCTGCGTCTCGTCGCCGAGGCCCTCGATGTGACGATGCCCGGCCGCTTCGTGCCCCCCGGCCGCCT
GCATCCGCTCACCGCCACCACCGACCGGATCGTCGATGTGTTCGTGGGTCTCGGGTTCACCGTCGCCTCCGGACCGCAGA
TCGAGACCGAGTACTACAACTTCGAGGCCCTCAACACCCCTGCCGATCACCCCGCCCGCGACATGCAGGACACGTTTTAC
CTGAGCGACGGGCTGGTCCTGCGCACCCAGACTTCCTCGGTGCAAATTCGCTACATGGAAGAGAACGACCCGCCCGTGCG
CATCTGCGTGCCCGGCCGCGTCTACCGCCGCGATCAGGTGACCAACCGCCACTCGCCGGTCTTCCACCAGCTGGAGCTGC
TGGCGGTGGACGAGGAGATCACCTTCGGAGACCTCAAGGGCACCCTCACCTTCTTTACCCAGGAAATGTTCGGCGACCGG
CCGGTGCGCTTTCGGCCGAGTTTCTTTCCGTTCACCGAACCTTCGGCGGAGGTGGACGTGCAGTGCCGCTTCTGCGACGG
CAAGGGTTGCCGCACCTGCTCCCACACCGGCTGGCTGGAGATCGCGGGCTGCGGCATGGTGGACCCGAACGTCTTTCAGG
CGGTGGGCTACAACCCCGAGAAGGTCCAGGGCTTTGCGGCGGGCATGGGCATCGAGCGCATCGCCATGCTGCTGTACGAC
ATCAACGACATTCGCCTGTTCTATACCAACGACCTGCGCTTCTTGCGGCAGTTTTAA

Upstream 100 bases:

>100_bases
ACTCCCGGACCTTTGACTTGTTACGATGCTTTCCCATCCTAGCACCGAGGGCAAGGGGTCACGGTGCGCGATAAGATTGT
TCAGGTACTTCGATACTCCT

Downstream 100 bases:

>100_bases
CCGATGGACACCACGCCCCTCACCCCGCTCACCAAGGGCATCACCCTCTACCAGCACATCCTTTCCCAGCAGGCGCTCAA
TCCCGACGCCACCGGCGAAT

Product: phenylalanyl-tRNA synthetase alpha chain

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQQQLDALQLEALKAIREAADLTILERIRVDFLGKKGKLSALLGGMARLPAEERPVVGALVNQVKARVEEALAAQQTHLQ
DQLIELRLVAEALDVTMPGRFVPPGRLHPLTATTDRIVDVFVGLGFTVASGPQIETEYYNFEALNTPADHPARDMQDTFY
LSDGLVLRTQTSSVQIRYMEENDPPVRICVPGRVYRRDQVTNRHSPVFHQLELLAVDEEITFGDLKGTLTFFTQEMFGDR
PVRFRPSFFPFTEPSAEVDVQCRFCDGKGCRTCSHTGWLEIAGCGMVDPNVFQAVGYNPEKVQGFAAGMGIERIAMLLYD
INDIRLFYTNDLRFLRQF

Sequences:

>Translated_338_residues
MQQQLDALQLEALKAIREAADLTILERIRVDFLGKKGKLSALLGGMARLPAEERPVVGALVNQVKARVEEALAAQQTHLQ
DQLIELRLVAEALDVTMPGRFVPPGRLHPLTATTDRIVDVFVGLGFTVASGPQIETEYYNFEALNTPADHPARDMQDTFY
LSDGLVLRTQTSSVQIRYMEENDPPVRICVPGRVYRRDQVTNRHSPVFHQLELLAVDEEITFGDLKGTLTFFTQEMFGDR
PVRFRPSFFPFTEPSAEVDVQCRFCDGKGCRTCSHTGWLEIAGCGMVDPNVFQAVGYNPEKVQGFAAGMGIERIAMLLYD
INDIRLFYTNDLRFLRQF
>Mature_338_residues
MQQQLDALQLEALKAIREAADLTILERIRVDFLGKKGKLSALLGGMARLPAEERPVVGALVNQVKARVEEALAAQQTHLQ
DQLIELRLVAEALDVTMPGRFVPPGRLHPLTATTDRIVDVFVGLGFTVASGPQIETEYYNFEALNTPADHPARDMQDTFY
LSDGLVLRTQTSSVQIRYMEENDPPVRICVPGRVYRRDQVTNRHSPVFHQLELLAVDEEITFGDLKGTLTFFTQEMFGDR
PVRFRPSFFPFTEPSAEVDVQCRFCDGKGCRTCSHTGWLEIAGCGMVDPNVFQAVGYNPEKVQGFAAGMGIERIAMLLYD
INDIRLFYTNDLRFLRQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=289, Percent_Identity=33.5640138408304, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=31.0861423220974, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1788007, Length=337, Percent_Identity=50.7418397626113, Blast_Score=338, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI32566635, Length=256, Percent_Identity=34.375, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=29.0566037735849, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32563657, Length=265, Percent_Identity=29.0566037735849, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=30.5970149253731, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=97, Percent_Identity=42.2680412371134, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=29.757785467128, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=29.1512915129151, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_GLOVI (Q7NGP0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926074.1
- ProteinModelPortal:   Q7NGP0
- SMR:   Q7NGP0
- GeneID:   2601529
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.3128
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- BioCyc:   GVIO251221:GLR3128-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38031; Mature: 38031

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQLDALQLEALKAIREAADLTILERIRVDFLGKKGKLSALLGGMARLPAEERPVVGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
VNQVKARVEEALAAQQTHLQDQLIELRLVAEALDVTMPGRFVPPGRLHPLTATTDRIVDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
FVGLGFTVASGPQIETEYYNFEALNTPADHPARDMQDTFYLSDGLVLRTQTSSVQIRYME
HHHCCEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEEE
ENDPPVRICVPGRVYRRDQVTNRHSPVFHQLELLAVDEEITFGDLKGTLTFFTQEMFGDR
CCCCCEEEEECCCCCCHHHCCCCCCCHHHEEEEEEECCCCEECCCCHHHHHHHHHHHCCC
PVRFRPSFFPFTEPSAEVDVQCRFCDGKGCRTCSHTGWLEIAGCGMVDPNVFQAVGYNPE
CEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHH
KVQGFAAGMGIERIAMLLYDINDIRLFYTNDLRFLRQF
HHHHHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHCC
>Mature Secondary Structure
MQQQLDALQLEALKAIREAADLTILERIRVDFLGKKGKLSALLGGMARLPAEERPVVGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
VNQVKARVEEALAAQQTHLQDQLIELRLVAEALDVTMPGRFVPPGRLHPLTATTDRIVDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
FVGLGFTVASGPQIETEYYNFEALNTPADHPARDMQDTFYLSDGLVLRTQTSSVQIRYME
HHHCCEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEEE
ENDPPVRICVPGRVYRRDQVTNRHSPVFHQLELLAVDEEITFGDLKGTLTFFTQEMFGDR
CCCCCEEEEECCCCCCHHHCCCCCCCHHHEEEEEEECCCCEECCCCHHHHHHHHHHHCCC
PVRFRPSFFPFTEPSAEVDVQCRFCDGKGCRTCSHTGWLEIAGCGMVDPNVFQAVGYNPE
CEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHH
KVQGFAAGMGIERIAMLLYDINDIRLFYTNDLRFLRQF
HHHHHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292