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The map label for this gene is rfaF [H]

Identifier: 37522681

GI number: 37522681

Start: 3315225

End: 3316211

Strand: Reverse

Name: rfaF [H]

Synonym: gll3112

Alternate gene names: 37522681

Gene position: 3316211-3315225 (Counterclockwise)

Preceding gene: 37522682

Following gene: 37522680

Centisome position: 71.18

GC content: 66.06

Gene sequence:

>987_bases
ATGACCCGCATCCTTGCTCTCAATCCCGGCGGCATCGGGGACCAGGTGCTCTTTTTTCCCACCCTGCGGGGACTGCGCGA
GCGCTTCGCCCAGTCGCGCATCGAGGTTGTCGTCGAACCCCGTTCGCAGGGCGCCTACCGGGTATGCCCGAGTGTCAACG
AAACGCTGACCTTCGACTTTAAGGGCGACCCGAGTCTGGCGGACTGGATGAATCTGATTGGCATCCTGCGCGACCGGCGC
TACGACGCCGTGCTCTCGGTGGGCAGCTCCAGCCTCGTCGCTCTGCTGTTGTGGATGACCGGCATCCCGAAGCGGGTAGG
CTACAGCGCCTGGATCACCGAACGGTTTTTGACCGACTCGGTGCCGCTGAACCGCGATCAGTACGCAGCGCAGATGTACC
ACGACCTGCTGCAGGGTTTTGGCATCCAGCGCGCCTTCACCCCGCCCCAGGCTGCGGTCTACTCCGAGGACGAGCGCTGG
GCCACCCAGGCCCTTGCCTCGCTGGGCGGGCGCAAGCCCCTGTTGCTGCACCCGGGGGCCAGCAAGCTCGCCGAACTGAA
GGGCATCCGCAAGCTCTACCCCGCCGCCCAATGGGCGCAGGTGGTGCAGAAACTGCTCGTCAAAGAAGCGGACCTGCCGT
TTTTCGTCGTCCAGGGGCCGGAGGACAGCGAACTGGTGACGGCCATCAAGGGGGAACTGGCGGATCAGGCCCGCTTTGTC
CAGCCCCCGGACGTGGGGAAGCTCACCGCCCTCATCGAGCGCTCGCGCCTGCTGTTGTGCGTCGACTCGGCACCGATGCA
CCTGGCGGTGGCCACCGGCACTCCCCTGGTCGCTCTGTTTGGCCCTACCCTGCCCACCAAGCTGCTGCCGGAGGAGCCTA
AGTACGTCGCCCTCGTTTCACCCGAGCGCGACAAAGTCGAGCGCATCCCGGTCGAGACGGTTGCCGCCGCGGCCATCAAG
CTCTTGGCCAAAACGCCGGTCGCCTGA

Upstream 100 bases:

>100_bases
GTGGCCCACGGCATCTACGTCTTCGGAATTGCCCGCTACAGCGAGACTTGAAGATTGCCGCCTTAGAATGGGTCTTGGTT
AACGAGGCTTCGCAACACCC

Downstream 100 bases:

>100_bases
AAGCCAATTGGGAGTGGACAATGGAGCTAAGCGGGCTCGAACCGCTGACCTCCGCAATGCCATTGCGGCGCTCTACCAGC
TGAGCTATAGCCCCAGACGT

Product: heptosyltransferase

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTRILALNPGGIGDQVLFFPTLRGLRERFAQSRIEVVVEPRSQGAYRVCPSVNETLTFDFKGDPSLADWMNLIGILRDRR
YDAVLSVGSSSLVALLLWMTGIPKRVGYSAWITERFLTDSVPLNRDQYAAQMYHDLLQGFGIQRAFTPPQAAVYSEDERW
ATQALASLGGRKPLLLHPGASKLAELKGIRKLYPAAQWAQVVQKLLVKEADLPFFVVQGPEDSELVTAIKGELADQARFV
QPPDVGKLTALIERSRLLLCVDSAPMHLAVATGTPLVALFGPTLPTKLLPEEPKYVALVSPERDKVERIPVETVAAAAIK
LLAKTPVA

Sequences:

>Translated_328_residues
MTRILALNPGGIGDQVLFFPTLRGLRERFAQSRIEVVVEPRSQGAYRVCPSVNETLTFDFKGDPSLADWMNLIGILRDRR
YDAVLSVGSSSLVALLLWMTGIPKRVGYSAWITERFLTDSVPLNRDQYAAQMYHDLLQGFGIQRAFTPPQAAVYSEDERW
ATQALASLGGRKPLLLHPGASKLAELKGIRKLYPAAQWAQVVQKLLVKEADLPFFVVQGPEDSELVTAIKGELADQARFV
QPPDVGKLTALIERSRLLLCVDSAPMHLAVATGTPLVALFGPTLPTKLLPEEPKYVALVSPERDKVERIPVETVAAAAIK
LLAKTPVA
>Mature_327_residues
TRILALNPGGIGDQVLFFPTLRGLRERFAQSRIEVVVEPRSQGAYRVCPSVNETLTFDFKGDPSLADWMNLIGILRDRRY
DAVLSVGSSSLVALLLWMTGIPKRVGYSAWITERFLTDSVPLNRDQYAAQMYHDLLQGFGIQRAFTPPQAAVYSEDERWA
TQALASLGGRKPLLLHPGASKLAELKGIRKLYPAAQWAQVVQKLLVKEADLPFFVVQGPEDSELVTAIKGELADQARFVQ
PPDVGKLTALIERSRLLLCVDSAPMHLAVATGTPLVALFGPTLPTKLLPEEPKYVALVSPERDKVERIPVETVAAAAIKL
LAKTPVA

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=296, Percent_Identity=26.0135135135135, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 36019; Mature: 35888

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRILALNPGGIGDQVLFFPTLRGLRERFAQSRIEVVVEPRSQGAYRVCPSVNETLTFDF
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCEEEEEE
KGDPSLADWMNLIGILRDRRYDAVLSVGSSSLVALLLWMTGIPKRVGYSAWITERFLTDS
CCCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCHHHCHHHHHHHHHHHCC
VPLNRDQYAAQMYHDLLQGFGIQRAFTPPQAAVYSEDERWATQALASLGGRKPLLLHPGA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCEEECCCH
SKLAELKGIRKLYPAAQWAQVVQKLLVKEADLPFFVVQGPEDSELVTAIKGELADQARFV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
QPPDVGKLTALIERSRLLLCVDSAPMHLAVATGTPLVALFGPTLPTKLLPEEPKYVALVS
CCCCCHHHHHHHHCCEEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEC
PERDKVERIPVETVAAAAIKLLAKTPVA
CCHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRILALNPGGIGDQVLFFPTLRGLRERFAQSRIEVVVEPRSQGAYRVCPSVNETLTFDF
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCEEEEEE
KGDPSLADWMNLIGILRDRRYDAVLSVGSSSLVALLLWMTGIPKRVGYSAWITERFLTDS
CCCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCHHHCHHHHHHHHHHHCC
VPLNRDQYAAQMYHDLLQGFGIQRAFTPPQAAVYSEDERWATQALASLGGRKPLLLHPGA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCEEECCCH
SKLAELKGIRKLYPAAQWAQVVQKLLVKEADLPFFVVQGPEDSELVTAIKGELADQARFV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
QPPDVGKLTALIERSRLLLCVDSAPMHLAVATGTPLVALFGPTLPTKLLPEEPKYVALVS
CCCCCHHHHHHHHCCEEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEC
PERDKVERIPVETVAAAAIKLLAKTPVA
CCHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]