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The map label for this gene is sigA [H]

Identifier: 37522577

GI number: 37522577

Start: 3205144

End: 3206061

Strand: Reverse

Name: sigA [H]

Synonym: gll3008

Alternate gene names: 37522577

Gene position: 3206061-3205144 (Counterclockwise)

Preceding gene: 37522578

Following gene: 37522576

Centisome position: 68.81

GC content: 62.2

Gene sequence:

>918_bases
ATGGAGAAAGACATGTCATCTTTGGTCAAAATTCAGCTTTCCGACCCCCAGCAGCGGCGCGGTCGGACCAGCCAGGCCTC
TCTTGAAGATTCCGTCGGCCAGTTTTTGCGCGAAATGTCCCGCTATCCGCTGCTCACTTCCGAGCAAGAGGTCGAGCTGG
CCCAGGTAATCGCCACCGGGGGGTCGGAGGCGGAAGCGGCCAAGCGCCGCCTAGTGCGGGCGAACCTGCGGCTGGTCGTC
TCGATCGCCAAAAAATATCTCAACCGCGGCGTGCCGTTTCTGGATCTGATTCAAGAAGGGGCCATCGGCCTGATGCGGGC
GGCGGAGAAGTTTGACTTCGAGCGCGGCTTCAAGTTCTCGACCTACGCCTACTGGTGGATCCGCCAGGGGATCACCCGCG
CCATCGCCTCGCAGTCGCGCACGGTGCGCCTGCCGGTGCACATGGTGGAGAAGCTCAACCAGGTCAAGCGCGTTCGCCGA
CAACTCACCCAGGAACTGGCCCGCAAACCCACCAAGCCCGAGATGGCCGAGGCCCTTGAACTTGACATCGACAAGCTCGA
TGAGATTCTCGATGCCGGTCGGCGCACCCTGTCGCTGCACGTCAAAGTCGGCAAAGAAGAAGACACCGAGTTGCTGCAGC
TCATCGAAGACAGCGACACGATCTCCCCGTCGGAGGTGCTCGATCACAATTTGCTTGTCGAGCAGATCGAGTCGCTGCTG
CACCTGTTGACTCCCCGCGAGCGCGACATCATCGAGTTGCGCTTCGGGCTGGCCGACGGCAACAGCTACACCCTGGCCGA
CATCGGCGAGATGTATAACCTCTCGCGCGAGCGGGTGCGCCAGATCCAGGCCAAGGCGATGCGCAAGCTGCGCCACCCCC
GTCGTCAGGTGCTCCTGCGCGATTGGGCCGAGGAGTGA

Upstream 100 bases:

>100_bases
AAGCCGCAACACTTTTCTTTTTTGTTGACTGATTGGTTAAATTTTGTTAAATAAATTAGAGACCTTGAAGCGGATTTAAG
CGGTCTTGCCTTCGGAGTAT

Downstream 100 bases:

>100_bases
ATCTTAAAAGCGGCACCCTTCACCCTGCTGTACTGCAGGTGGAGGGTGCCAAAATGGGCGTTATGGATGAGCTGATCGAG
GCGGAACCTTACACGCTCGA

Product: group 2 sigma 70-type sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEKDMSSLVKIQLSDPQQRRGRTSQASLEDSVGQFLREMSRYPLLTSEQEVELAQVIATGGSEAEAAKRRLVRANLRLVV
SIAKKYLNRGVPFLDLIQEGAIGLMRAAEKFDFERGFKFSTYAYWWIRQGITRAIASQSRTVRLPVHMVEKLNQVKRVRR
QLTQELARKPTKPEMAEALELDIDKLDEILDAGRRTLSLHVKVGKEEDTELLQLIEDSDTISPSEVLDHNLLVEQIESLL
HLLTPRERDIIELRFGLADGNSYTLADIGEMYNLSRERVRQIQAKAMRKLRHPRRQVLLRDWAEE

Sequences:

>Translated_305_residues
MEKDMSSLVKIQLSDPQQRRGRTSQASLEDSVGQFLREMSRYPLLTSEQEVELAQVIATGGSEAEAAKRRLVRANLRLVV
SIAKKYLNRGVPFLDLIQEGAIGLMRAAEKFDFERGFKFSTYAYWWIRQGITRAIASQSRTVRLPVHMVEKLNQVKRVRR
QLTQELARKPTKPEMAEALELDIDKLDEILDAGRRTLSLHVKVGKEEDTELLQLIEDSDTISPSEVLDHNLLVEQIESLL
HLLTPRERDIIELRFGLADGNSYTLADIGEMYNLSRERVRQIQAKAMRKLRHPRRQVLLRDWAEE
>Mature_305_residues
MEKDMSSLVKIQLSDPQQRRGRTSQASLEDSVGQFLREMSRYPLLTSEQEVELAQVIATGGSEAEAAKRRLVRANLRLVV
SIAKKYLNRGVPFLDLIQEGAIGLMRAAEKFDFERGFKFSTYAYWWIRQGITRAIASQSRTVRLPVHMVEKLNQVKRVRR
QLTQELARKPTKPEMAEALELDIDKLDEILDAGRRTLSLHVKVGKEEDTELLQLIEDSDTISPSEVLDHNLLVEQIESLL
HLLTPRERDIIELRFGLADGNSYTLADIGEMYNLSRERVRQIQAKAMRKLRHPRRQVLLRDWAEE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=272, Percent_Identity=45.2205882352941, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=42.3664122137405, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=27.9411764705882, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=29.126213592233, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35158; Mature: 35158

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDMSSLVKIQLSDPQQRRGRTSQASLEDSVGQFLREMSRYPLLTSEQEVELAQVIATG
CCCCHHHHHEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
GSEAEAAKRRLVRANLRLVVSIAKKYLNRGVPFLDLIQEGAIGLMRAAEKFDFERGFKFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TYAYWWIRQGITRAIASQSRTVRLPVHMVEKLNQVKRVRRQLTQELARKPTKPEMAEALE
HHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LDIDKLDEILDAGRRTLSLHVKVGKEEDTELLQLIEDSDTISPSEVLDHNLLVEQIESLL
CCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HLLTPRERDIIELRFGLADGNSYTLADIGEMYNLSRERVRQIQAKAMRKLRHPRRQVLLR
HHHCCCCCCCEEEEEEECCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHH
DWAEE
HHCCC
>Mature Secondary Structure
MEKDMSSLVKIQLSDPQQRRGRTSQASLEDSVGQFLREMSRYPLLTSEQEVELAQVIATG
CCCCHHHHHEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
GSEAEAAKRRLVRANLRLVVSIAKKYLNRGVPFLDLIQEGAIGLMRAAEKFDFERGFKFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TYAYWWIRQGITRAIASQSRTVRLPVHMVEKLNQVKRVRRQLTQELARKPTKPEMAEALE
HHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LDIDKLDEILDAGRRTLSLHVKVGKEEDTELLQLIEDSDTISPSEVLDHNLLVEQIESLL
CCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HLLTPRERDIIELRFGLADGNSYTLADIGEMYNLSRERVRQIQAKAMRKLRHPRRQVLLR
HHHCCCCCCCEEEEEEECCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHH
DWAEE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]