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The map label for this gene is dhaAF [H]

Identifier: 37522378

GI number: 37522378

Start: 2988219

End: 2989079

Strand: Direct

Name: dhaAF [H]

Synonym: glr2809

Alternate gene names: 37522378

Gene position: 2988219-2989079 (Clockwise)

Preceding gene: 37522376

Following gene: 37522380

Centisome position: 64.14

GC content: 66.55

Gene sequence:

>861_bases
GTGCTCATCCGCGAAAGTACGGTCCGCGCCGGAGGCCGGCAATGGTTTTACCGCTCGGCGGGCAGCGAGTCTGGGCCGCC
CGTGCTGCTCCTGCACGGTCTGCCTTCCCACAGCCACGGTTGGCGGGAGGCGGTCCCGGTGCTGGCCGAGCGGGGTCTGT
GGGTGATCGCCGCCGACTGGGTGGGTTTTGGCGGTAGCGACAAACCGGACAAATCCGCCTTCAACTACACACCGGACGCT
TTTATTGCGGCGCTGGCAGCCTTTGTAGACGCCCTCGCCCTGGAGCGCTTCTCGCTGGTGGTGCAGGGATTTCTGGGCTC
AGCAGGCATCCAGTACGCCCTGCGCGAGCCGCGGCGCATCGCCCGGCTGGCCGTCATCAACGCCCCCATCGCCACGAGTG
CCAAATTGCCCTGGAACCTCCGGCAGCTGGGGTTGCCCCTAGTGGGTGAGATGCTCACCCAAAATCCGCTCTCGGTGGAT
CAGACCCTCGAAGGGGGAGGTTATACGGTCATTTCCAACGGCGATCTAGGCGCCTTTCGCCGCCCCTACAGCGAGAGCGG
CGACGCCGGACGGGCATTGATGCTCACCGTGCGCAATCTGGATCTGCCCCGGGCGATGGCGGAGATCGAAGCGGGCCTGA
CTCCCTGGCGTCAGAACGGTCCGGTGCCCACCCCCTTTGCGCTCGCCCTCATCTGGGGTCTGCGCGACCGCTACCTGGGT
GAGGCGATGGCCAAAAGCTTTGTGGCGGCCCACCCGCAGGTCGAATTTTTTCCGGTCGCCCAGGCCGGTCACTACCCGCT
GGAGCAAGACCCCGCGGCGGTCAACCAGGCCCTGGTGCGCTTTGTCGCTCAGCCGCGGTAG

Upstream 100 bases:

>100_bases
CACAAACGCCCTAAAGTTGACCTACCAACCGCCGTGACAAGCTATGGGTAAGCATTTATCAGCAACGGTCGCCGCACCGT
CCAGGAACGAGAGCTAGCCC

Downstream 100 bases:

>100_bases
GTACCGGAGTTGAGGGTGACGCTGCCTACCCCCCAGCGGGACACCAGCTTTTCGAGCAGAAGGCGGCCCGGGGCGGCCAG
ATCGCCGTCCTCACCCACCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLIRESTVRAGGRQWFYRSAGSESGPPVLLLHGLPSHSHGWREAVPVLAERGLWVIAADWVGFGGSDKPDKSAFNYTPDA
FIAALAAFVDALALERFSLVVQGFLGSAGIQYALREPRRIARLAVINAPIATSAKLPWNLRQLGLPLVGEMLTQNPLSVD
QTLEGGGYTVISNGDLGAFRRPYSESGDAGRALMLTVRNLDLPRAMAEIEAGLTPWRQNGPVPTPFALALIWGLRDRYLG
EAMAKSFVAAHPQVEFFPVAQAGHYPLEQDPAAVNQALVRFVAQPR

Sequences:

>Translated_286_residues
MLIRESTVRAGGRQWFYRSAGSESGPPVLLLHGLPSHSHGWREAVPVLAERGLWVIAADWVGFGGSDKPDKSAFNYTPDA
FIAALAAFVDALALERFSLVVQGFLGSAGIQYALREPRRIARLAVINAPIATSAKLPWNLRQLGLPLVGEMLTQNPLSVD
QTLEGGGYTVISNGDLGAFRRPYSESGDAGRALMLTVRNLDLPRAMAEIEAGLTPWRQNGPVPTPFALALIWGLRDRYLG
EAMAKSFVAAHPQVEFFPVAQAGHYPLEQDPAAVNQALVRFVAQPR
>Mature_286_residues
MLIRESTVRAGGRQWFYRSAGSESGPPVLLLHGLPSHSHGWREAVPVLAERGLWVIAADWVGFGGSDKPDKSAFNYTPDA
FIAALAAFVDALALERFSLVVQGFLGSAGIQYALREPRRIARLAVINAPIATSAKLPWNLRQLGLPLVGEMLTQNPLSVD
QTLEGGGYTVISNGDLGAFRRPYSESGDAGRALMLTVRNLDLPRAMAEIEAGLTPWRQNGPVPTPFALALIWGLRDRYLG
EAMAKSFVAAHPQVEFFPVAQAGHYPLEQDPAAVNQALVRFVAQPR

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324392, Length=120, Percent_Identity=32.5, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 31005; Mature: 31005

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRESTVRAGGRQWFYRSAGSESGPPVLLLHGLPSHSHGWREAVPVLAERGLWVIAADW
CCCCCCHHHCCCHHEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECC
VGFGGSDKPDKSAFNYTPDAFIAALAAFVDALALERFSLVVQGFLGSAGIQYALREPRRI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLAVINAPIATSAKLPWNLRQLGLPLVGEMLTQNPLSVDQTLEGGGYTVISNGDLGAFR
HHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCHHHEECCCCEEEEECCCCHHHC
RPYSESGDAGRALMLTVRNLDLPRAMAEIEAGLTPWRQNGPVPTPFALALIWGLRDRYLG
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EAMAKSFVAAHPQVEFFPVAQAGHYPLEQDPAAVNQALVRFVAQPR
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLIRESTVRAGGRQWFYRSAGSESGPPVLLLHGLPSHSHGWREAVPVLAERGLWVIAADW
CCCCCCHHHCCCHHEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECC
VGFGGSDKPDKSAFNYTPDAFIAALAAFVDALALERFSLVVQGFLGSAGIQYALREPRRI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLAVINAPIATSAKLPWNLRQLGLPLVGEMLTQNPLSVDQTLEGGGYTVISNGDLGAFR
HHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCHHHEECCCCEEEEECCCCHHHC
RPYSESGDAGRALMLTVRNLDLPRAMAEIEAGLTPWRQNGPVPTPFALALIWGLRDRYLG
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EAMAKSFVAAHPQVEFFPVAQAGHYPLEQDPAAVNQALVRFVAQPR
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]