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The map label for this gene is ilvB [H]

Identifier: 37522373

GI number: 37522373

Start: 2981581

End: 2983218

Strand: Reverse

Name: ilvB [H]

Synonym: gvip386

Alternate gene names: 37522373

Gene position: 2983218-2981581 (Counterclockwise)

Preceding gene: 37522374

Following gene: 37522372

Centisome position: 64.03

GC content: 62.52

Gene sequence:

>1638_bases
ATGAACACAGCCGAATTGTTGGTGCAGTGCCTGGAGAACGAAGGTGTCCGCTACATCTTCGGCTTGCCCGGCGAGGAGAA
CATGCAGTTTCTGGAGGCGCTGCAAAGCTCATCTATTCAGTTTGTCACCACCCGCCACGAACAGGGCGCCGCCTTCATGG
CCGATGTCTACGGGCGGCTGACCGGCCGGGCGGGGGTGTGCCTTTCGACCCTGGGTCCCGGTGCGACCAATTTGATGACC
GGCGTGGCCGACGCCAACCTCGATCGCGCCCCACTGGTGGCGATCACCGGTCAGGTGGGTACCGATCGCATGCACGTCGA
CGCCCACCAGTACCTCGACTTGGTGGCGATGTTCGCTCCGGTGACCAAGTGGAGCACCCATATCGTCCGCCCGAGCAACA
CCCCCGAAATCGTCCGCAAGGCGTTCAAGATCGCCCAGGCCGAAAAACCGGGGGCAGTGCACATCGAACTACCGGAAAAC
ATCGCTTCGATGGCGGCGATCGGCGAGCCGCTGCGCATCAGCGACAATCACGACATATTCGCTTCATTCAGCAGCATCAC
CCGGGCGGCCGAGCTGATTTCCCAGGCCGACAACCCGCTTATCCTGGTGGGCAACGGCGCCATCCGCTGCAATGCGAGTG
CCGCGCTCACCGAGTTCGCCGCCCGCCTGAATATCCCGGTGGCCAATACCTTCATGGGCAAGGGGGTTATTCCTTATACC
CATCCGCTGGCGCTGTGGGCGGTGGGCCTGCAGCAGCGCGACTACATCAGCTGCGGCTTCGACCGCGCCGACCTGGTGAT
CGCCATCGGCTACGACCACATCGAGTACTCGCCCAAAAAGTGGAACCCCATGGGCGACATCCCGATCGTCCATGTCGGCC
GCACCGCCGCGGAAGTCGACAGCAGCTATATCCCGCAAGTCGAAGTGGTGGGCGACATCTCCGACAGCCTGCAGGAGATC
CTGTCGCGCGCCGACCGCCAGGGCAAACCCGACCCCTACGCCATCCAACTGCGCGACGACATCCGCGCCGAGTACACCGA
GTACGCCCACGACACCGGCTATCCCGTCAAACCCCAAAAAATAATCTACGACCTGCGCCAGGTGCTGGGCTCGGAGGATA
TCTTGATCTCCGACGTCGGCGCCCACAAGATGTGGGTGGCCCGCCACTACCACTGCGACCGCCCCAACACCTGCATCATC
TCCAACGGCTTCGCCGCTATGGGCATCGCCATCCCGGGCGCTGTCGCCGCCAAACTCGTCCACCCCCACCGCAAAGTGGT
GGCCGTCACCGGTGACGGCGGCTTCATGATGAACATGCAGGAACTGGAGACGGCCCTGCGCATCGGTACGCCCTTTGTGA
CGCTTATCTTCAACGACGGCGGCTACGGCCTCATCGAATGGAAGCAACAGACCCACTTCAACCGCTCCGCCTTCGTGCGC
TTCGGCAACCCCGACTTTGTGCGCCTTGCCGAGAGTATGGGTCTTAAGGGCTACCGCGTCGAGGCGGGCGACGAGTTGTT
GCCCATCCTCAAAACTGCCCTCGAACAGGAGGTTCCGGCCATCATCGACTGTCCGGTGGACTACCGGGAGAACATGGAAC
TCTCGCAGCGGGTCATGCAACTCAGTTGCGCCGTTTAA

Upstream 100 bases:

>100_bases
CCCGTCTACCCTTCGGCGGCACCAAGCTCTCCGGCTACGGCCGCGAACTCAGCCGCGAAGGCATGCTCGAATTTGTCAAT
ATCAAAACGGTGTGGGAGTT

Downstream 100 bases:

>100_bases
CCTGCTGCTTCTCCCAATGGCCGGGTTGACTTTAAGTCATTACGGTTTCATCCGTTCGGGGGGAATTAGGCAGTCCAAGA
ATTATCTGGCGCCCTTGGCA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNTAELLVQCLENEGVRYIFGLPGEENMQFLEALQSSSIQFVTTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDRAPLVAITGQVGTDRMHVDAHQYLDLVAMFAPVTKWSTHIVRPSNTPEIVRKAFKIAQAEKPGAVHIELPEN
IASMAAIGEPLRISDNHDIFASFSSITRAAELISQADNPLILVGNGAIRCNASAALTEFAARLNIPVANTFMGKGVIPYT
HPLALWAVGLQQRDYISCGFDRADLVIAIGYDHIEYSPKKWNPMGDIPIVHVGRTAAEVDSSYIPQVEVVGDISDSLQEI
LSRADRQGKPDPYAIQLRDDIRAEYTEYAHDTGYPVKPQKIIYDLRQVLGSEDILISDVGAHKMWVARHYHCDRPNTCII
SNGFAAMGIAIPGAVAAKLVHPHRKVVAVTGDGGFMMNMQELETALRIGTPFVTLIFNDGGYGLIEWKQQTHFNRSAFVR
FGNPDFVRLAESMGLKGYRVEAGDELLPILKTALEQEVPAIIDCPVDYRENMELSQRVMQLSCAV

Sequences:

>Translated_545_residues
MNTAELLVQCLENEGVRYIFGLPGEENMQFLEALQSSSIQFVTTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDRAPLVAITGQVGTDRMHVDAHQYLDLVAMFAPVTKWSTHIVRPSNTPEIVRKAFKIAQAEKPGAVHIELPEN
IASMAAIGEPLRISDNHDIFASFSSITRAAELISQADNPLILVGNGAIRCNASAALTEFAARLNIPVANTFMGKGVIPYT
HPLALWAVGLQQRDYISCGFDRADLVIAIGYDHIEYSPKKWNPMGDIPIVHVGRTAAEVDSSYIPQVEVVGDISDSLQEI
LSRADRQGKPDPYAIQLRDDIRAEYTEYAHDTGYPVKPQKIIYDLRQVLGSEDILISDVGAHKMWVARHYHCDRPNTCII
SNGFAAMGIAIPGAVAAKLVHPHRKVVAVTGDGGFMMNMQELETALRIGTPFVTLIFNDGGYGLIEWKQQTHFNRSAFVR
FGNPDFVRLAESMGLKGYRVEAGDELLPILKTALEQEVPAIIDCPVDYRENMELSQRVMQLSCAV
>Mature_545_residues
MNTAELLVQCLENEGVRYIFGLPGEENMQFLEALQSSSIQFVTTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDRAPLVAITGQVGTDRMHVDAHQYLDLVAMFAPVTKWSTHIVRPSNTPEIVRKAFKIAQAEKPGAVHIELPEN
IASMAAIGEPLRISDNHDIFASFSSITRAAELISQADNPLILVGNGAIRCNASAALTEFAARLNIPVANTFMGKGVIPYT
HPLALWAVGLQQRDYISCGFDRADLVIAIGYDHIEYSPKKWNPMGDIPIVHVGRTAAEVDSSYIPQVEVVGDISDSLQEI
LSRADRQGKPDPYAIQLRDDIRAEYTEYAHDTGYPVKPQKIIYDLRQVLGSEDILISDVGAHKMWVARHYHCDRPNTCII
SNGFAAMGIAIPGAVAAKLVHPHRKVVAVTGDGGFMMNMQELETALRIGTPFVTLIFNDGGYGLIEWKQQTHFNRSAFVR
FGNPDFVRLAESMGLKGYRVEAGDELLPILKTALEQEVPAIIDCPVDYRENMELSQRVMQLSCAV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=552, Percent_Identity=26.9927536231884, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI21361361, Length=553, Percent_Identity=25.1356238698011, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=557, Percent_Identity=32.3159784560144, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1790104, Length=537, Percent_Identity=30.7262569832402, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1786717, Length=571, Percent_Identity=27.1453590192644, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=24.0808823529412, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=26.7148014440433, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=27.0103092783505, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=27.0103092783505, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=592, Percent_Identity=24.4932432432432, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=31.0714285714286, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=26.5560165975104, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6323163, Length=524, Percent_Identity=23.0916030534351, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=526, Percent_Identity=22.6235741444867, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=523, Percent_Identity=22.7533460803059, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320123, Length=497, Percent_Identity=23.943661971831, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922626, Length=556, Percent_Identity=26.6187050359712, Blast_Score=170, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59745; Mature: 59745

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAELLVQCLENEGVRYIFGLPGEENMQFLEALQSSSIQFVTTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
TGRAGVCLSTLGPGATNLMTGVADANLDRAPLVAITGQVGTDRMHVDAHQYLDLVAMFAP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECHHHHHHHHHHHHH
VTKWSTHIVRPSNTPEIVRKAFKIAQAEKPGAVHIELPENIASMAAIGEPLRISDNHDIF
HHHCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCEEECCCCCHH
ASFSSITRAAELISQADNPLILVGNGAIRCNASAALTEFAARLNIPVANTFMGKGVIPYT
HHHHHHHHHHHHHHCCCCCEEEEECCEEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCCC
HPLALWAVGLQQRDYISCGFDRADLVIAIGYDHIEYSPKKWNPMGDIPIVHVGRTAAEVD
CHHHHHHHCCCCCCCEECCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEECCHHHHCC
SSYIPQVEVVGDISDSLQEILSRADRQGKPDPYAIQLRDDIRAEYTEYAHDTGYPVKPQK
CCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCHHH
IIYDLRQVLGSEDILISDVGAHKMWVARHYHCDRPNTCIISNGFAAMGIAIPGAVAAKLV
HHHHHHHHHCCCCEEEECCCCCCEEEEHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHH
HPHRKVVAVTGDGGFMMNMQELETALRIGTPFVTLIFNDGGYGLIEWKQQTHFNRSAFVR
CCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHCCCCCEEEEE
FGNPDFVRLAESMGLKGYRVEAGDELLPILKTALEQEVPAIIDCPVDYRENMELSQRVMQ
ECCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHH
LSCAV
HHCCC
>Mature Secondary Structure
MNTAELLVQCLENEGVRYIFGLPGEENMQFLEALQSSSIQFVTTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
TGRAGVCLSTLGPGATNLMTGVADANLDRAPLVAITGQVGTDRMHVDAHQYLDLVAMFAP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECHHHHHHHHHHHHH
VTKWSTHIVRPSNTPEIVRKAFKIAQAEKPGAVHIELPENIASMAAIGEPLRISDNHDIF
HHHCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCEEECCCCCHH
ASFSSITRAAELISQADNPLILVGNGAIRCNASAALTEFAARLNIPVANTFMGKGVIPYT
HHHHHHHHHHHHHHCCCCCEEEEECCEEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCCC
HPLALWAVGLQQRDYISCGFDRADLVIAIGYDHIEYSPKKWNPMGDIPIVHVGRTAAEVD
CHHHHHHHCCCCCCCEECCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEECCHHHHCC
SSYIPQVEVVGDISDSLQEILSRADRQGKPDPYAIQLRDDIRAEYTEYAHDTGYPVKPQK
CCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCHHH
IIYDLRQVLGSEDILISDVGAHKMWVARHYHCDRPNTCIISNGFAAMGIAIPGAVAAKLV
HHHHHHHHHCCCCEEEECCCCCCEEEEHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHH
HPHRKVVAVTGDGGFMMNMQELETALRIGTPFVTLIFNDGGYGLIEWKQQTHFNRSAFVR
CCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHCCCCCEEEEE
FGNPDFVRLAESMGLKGYRVEAGDELLPILKTALEQEVPAIIDCPVDYRENMELSQRVMQ
ECCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHH
LSCAV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]