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The map label for this gene is purD [H]

Identifier: 37522348

GI number: 37522348

Start: 2959165

End: 2960445

Strand: Direct

Name: purD [H]

Synonym: gvip382

Alternate gene names: 37522348

Gene position: 2959165-2960445 (Clockwise)

Preceding gene: 37522346

Following gene: 37522350

Centisome position: 63.51

GC content: 64.25

Gene sequence:

>1281_bases
ATGGCATTGAAGATATTGGTGGTCGGCAATGGCGGTCGGGAGCACTGCCTGGCCTGGGCGCTCGCCCGCTCCGAGGGGGT
GGGCCGGATCTGGGTCGCCCCGGGTAACGGCGGCACCGCCACCGAATCCAAGTGCGCCAATCTGGCCGTCTCGCCGCTCG
ATTTTGCGGCCCTGGCGGATTTTGCCCACTCTGAGCGCGTCGATCTGACCGTGGTCGGCCCCGAGGCACCCCTGGCTGCG
GGCATCGTCGATTACTTCCAGGCTGAGGGACTGATTATTTTCGGCCCCACGCGCGAAGGGGCACAACTCGAAGCGAGCAA
AGCCTGGGCCAAACACTTGATGCACGAAGCCGGGGTACCCACCGCCGCCGCCAGAAGTTTTGCCGATATCGAGGCGGCCC
GCGCCTACGTGCGCGAACTGGGGGCACCTCTGGTGGTCAAAGCCGACGGTCTTGCCGCCGGCAAAGGGGTGACCGTGGCG
ATGGAGACCGCCGAGGCCCTGGCTGCCCTCGACGAGTTGCCGCGCTTCGGGGCGGCGGGGGCGACCGTGGTCATCGAAGA
ATTTATGGCCGGGGAAGAAGCTTCGGTACTCGCCTTCACCGACGGCAAGACCATCCGGGCGATGGTGCCCGCCCAAGATC
ACAAGCGTGTAGGCGAGGGCGACACCGGCCCCAACACCGGCGGCATGGGTGCCTACGCCCCCGCCCCACTCGTGGACGCC
GCCCTGATGCGTCAAATCGAGGAACGCATTCTTGCTCCTACACTCACCGCTTTGCAAAAGCGGAGTATCGACTACCGGGG
CGTACTGTACGCCGGACTGATGATTGGTCCTGACAGCTCGGTACGGGTAATCGAATTCAACTGCCGCCTGGGCGATCCCG
AGACCCAGGTGGTCCTGCCGCTGCTGGAAACCCCTTTAGAAGAAGTGCTCCTGGCTACCGCCCAGGGCAGTCTCGATCGG
GTGGATTTGCAATGGAAACCGGGTGCTGCCGCCTGCGTGGTACTCGCAGCGGGAGGTTACCCGGGTGACTACCGCCAGGG
CGATGTGATTACCGGCCTGGAGGAGGCCCGTGCCCAGGGCGGCCTCGTCTTCCACAGCGGAACTCGCCGGGAGAAAGAAC
GCATCTTGAGCGCCGGGGGCCGCATCCTGGCAGTAACTGCATTGGGCGATGCCCTCGAAAGCGCCATCGCAAGCGCTTAC
CAGGTCGTCAATTGCATTGCCGTCAAAGATGTTCATTACCGCCGTGACATCGGTTGGCGGGCTTTGCAGCTATGCAAGTA
A

Upstream 100 bases:

>100_bases
ACGGCGACGGTATTTCCACATTGTTGCAACAGGACGGCCCCGCCGCGAAGCCCTCTCCCTTGCGATAATCGTGACTGGTC
TTTTGGTGGAATCGGGTTCG

Downstream 100 bases:

>100_bases
GTCTGTGCCATTTCATCAAGCACCTGTGTTCTGAAATGGCGAGGGGTACTATGGAATTTTCCAAGTACAGAAGGCGTCGC
AATTGTTCACCCTCACTTAG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MALKILVVGNGGREHCLAWALARSEGVGRIWVAPGNGGTATESKCANLAVSPLDFAALADFAHSERVDLTVVGPEAPLAA
GIVDYFQAEGLIIFGPTREGAQLEASKAWAKHLMHEAGVPTAAARSFADIEAARAYVRELGAPLVVKADGLAAGKGVTVA
METAEALAALDELPRFGAAGATVVIEEFMAGEEASVLAFTDGKTIRAMVPAQDHKRVGEGDTGPNTGGMGAYAPAPLVDA
ALMRQIEERILAPTLTALQKRSIDYRGVLYAGLMIGPDSSVRVIEFNCRLGDPETQVVLPLLETPLEEVLLATAQGSLDR
VDLQWKPGAAACVVLAAGGYPGDYRQGDVITGLEEARAQGGLVFHSGTRREKERILSAGGRILAVTALGDALESAIASAY
QVVNCIAVKDVHYRRDIGWRALQLCK

Sequences:

>Translated_426_residues
MALKILVVGNGGREHCLAWALARSEGVGRIWVAPGNGGTATESKCANLAVSPLDFAALADFAHSERVDLTVVGPEAPLAA
GIVDYFQAEGLIIFGPTREGAQLEASKAWAKHLMHEAGVPTAAARSFADIEAARAYVRELGAPLVVKADGLAAGKGVTVA
METAEALAALDELPRFGAAGATVVIEEFMAGEEASVLAFTDGKTIRAMVPAQDHKRVGEGDTGPNTGGMGAYAPAPLVDA
ALMRQIEERILAPTLTALQKRSIDYRGVLYAGLMIGPDSSVRVIEFNCRLGDPETQVVLPLLETPLEEVLLATAQGSLDR
VDLQWKPGAAACVVLAAGGYPGDYRQGDVITGLEEARAQGGLVFHSGTRREKERILSAGGRILAVTALGDALESAIASAY
QVVNCIAVKDVHYRRDIGWRALQLCK
>Mature_425_residues
ALKILVVGNGGREHCLAWALARSEGVGRIWVAPGNGGTATESKCANLAVSPLDFAALADFAHSERVDLTVVGPEAPLAAG
IVDYFQAEGLIIFGPTREGAQLEASKAWAKHLMHEAGVPTAAARSFADIEAARAYVRELGAPLVVKADGLAAGKGVTVAM
ETAEALAALDELPRFGAAGATVVIEEFMAGEEASVLAFTDGKTIRAMVPAQDHKRVGEGDTGPNTGGMGAYAPAPLVDAA
LMRQIEERILAPTLTALQKRSIDYRGVLYAGLMIGPDSSVRVIEFNCRLGDPETQVVLPLLETPLEEVLLATAQGSLDRV
DLQWKPGAAACVVLAAGGYPGDYRQGDVITGLEEARAQGGLVFHSGTRREKERILSAGGRILAVTALGDALESAIASAYQ
VVNCIAVKDVHYRRDIGWRALQLCK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=49.4145199063232, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=49.4145199063232, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=49.4145199063232, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=49.4145199063232, Blast_Score=394, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=51.2941176470588, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=42.080378250591, Blast_Score=308, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=41.5909090909091, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI62471619, Length=435, Percent_Identity=48.0459770114943, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=48.0459770114943, Blast_Score=376, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44685; Mature: 44553

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKILVVGNGGREHCLAWALARSEGVGRIWVAPGNGGTATESKCANLAVSPLDFAALAD
CEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHH
FAHSERVDLTVVGPEAPLAAGIVDYFQAEGLIIFGPTREGAQLEASKAWAKHLMHEAGVP
HHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCC
TAAARSFADIEAARAYVRELGAPLVVKADGLAAGKGVTVAMETAEALAALDELPRFGAAG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCC
ATVVIEEFMAGEEASVLAFTDGKTIRAMVPAQDHKRVGEGDTGPNTGGMGAYAPAPLVDA
HHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
ALMRQIEERILAPTLTALQKRSIDYRGVLYAGLMIGPDSSVRVIEFNCRLGDPETQVVLP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEHH
LLETPLEEVLLATAQGSLDRVDLQWKPGAAACVVLAAGGYPGDYRQGDVITGLEEARAQG
HHHCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCEECCHHHHHHCC
GLVFHSGTRREKERILSAGGRILAVTALGDALESAIASAYQVVNCIAVKDVHYRRDIGWR
CEEEECCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCHH
ALQLCK
HHHHCC
>Mature Secondary Structure 
ALKILVVGNGGREHCLAWALARSEGVGRIWVAPGNGGTATESKCANLAVSPLDFAALAD
EEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHH
FAHSERVDLTVVGPEAPLAAGIVDYFQAEGLIIFGPTREGAQLEASKAWAKHLMHEAGVP
HHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCC
TAAARSFADIEAARAYVRELGAPLVVKADGLAAGKGVTVAMETAEALAALDELPRFGAAG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCC
ATVVIEEFMAGEEASVLAFTDGKTIRAMVPAQDHKRVGEGDTGPNTGGMGAYAPAPLVDA
HHHHHHHHHCCCCCEEEEEECCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
ALMRQIEERILAPTLTALQKRSIDYRGVLYAGLMIGPDSSVRVIEFNCRLGDPETQVVLP
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCEEEHH
LLETPLEEVLLATAQGSLDRVDLQWKPGAAACVVLAAGGYPGDYRQGDVITGLEEARAQG
HHHCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCEECCHHHHHHCC
GLVFHSGTRREKERILSAGGRILAVTALGDALESAIASAYQVVNCIAVKDVHYRRDIGWR
CEEEECCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCHH
ALQLCK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]