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The map label for this gene is braE [H]

Identifier: 37522260

GI number: 37522260

Start: 2850775

End: 2851884

Strand: Direct

Name: braE [H]

Synonym: glr2691

Alternate gene names: 37522260

Gene position: 2850775-2851884 (Clockwise)

Preceding gene: 37522259

Following gene: 37522261

Centisome position: 61.19

GC content: 63.78

Gene sequence:

>1110_bases
ATGCTAAAATCCAGCCAATTTCGATGGGGTCCAATTGGTTCGGTGGCACAGCTTCCCATTCGGCAAACGATTTTTCAGTG
GAGCGAACGCCTGCCGGCACCGCTCAGGCGCTACGGGCTTTTGGGTGTGGGCCTGCTCATCCCGGCATTGATGCTGCTCA
CTTCCCAGCCGAGCTACCTGGCCAATGTCGCGGTGACCGTGGCGGTTTACATGGTGCTGGCCATGGGGCTCAACATCGTG
GTCGGCCTGGCGGGCCTGTTGGATCTGGGCTACATCGCCTTTTTCGCGGTGGGCGCCTATACCTACGCCATCGGGGCGCC
CCATGGCCTCAGCTTCTGGCTCGCCCTGCCGGTGGCTGCACTGCTTGCCGCCGGTTTCGGGGTGCTTCTGGGGGCACCGA
CTTTGCCCTTGCGCGGCGACTACCTGGCCATCGTCACCCTCGGTTTCGGCGAAATCATCCGCATCTCGCTCAACAACCTC
GACTGGCTCACCCGCGGTCCGGCGGGCATCAGCGGTGTACCCGCACCCGTGATCCCCTGGATAGGCGCCAAAGGCTTCAC
CTGGCTCACGCTCTATCAGCCGCTGCAACTCTATTTCATCGCGCTTGCCTTCGTCGCCTTTGTCTACTGGATGACCAGCC
GCCTGAAGCAGTCGCGCGTCGGCCGCGCCTGGGTAGCCATCCGCGAGGATGAGATTGCCGCCGCCGCCATGGGCGTCGAC
ACCGTCAAGCTCAAGCTCCTGGCCTTCGCCACCGGGGCGGCCCTGGCCGGCATGGTCGGGGTGCTCTTTGCCGTGCAGCT
CACCTATGTCTCCCCCGACAGCTTCACCCTGATCGAATCGGTGATGGTCGTCTCGATGGTGGTCCTGGGAGGGATGGGAT
CGGTGCCCGGGGTGGCCCTTGGGGCTTTGATCCTAATTGTCCTGCCGGAGGCGCTCCGGGGCTTTTCCGAGTACCGGCTG
CTGTTATTTGGCGCGGCGATGATCCTGGTGATGCTCATCCGTCCCCAGGGATTATTGGGCGAGCGGCAGGGCGTGCCGCC
CCGGCCTACAGGAGCCGGTCTTGAAGACGAAACGCCGCTCGAAGTCGAAAAGCGCGACCTGCCGGTGTAA

Upstream 100 bases:

>100_bases
GCGGGTGCGCGTCGACGGCGGTGCGGGCACCGTCGAAATCCTTGGCGCGGATCCTCACGCGGGGGAAAAACCTACAGCTT
GAACGTCCCGACGGCTGGGA

Downstream 100 bases:

>100_bases
CACCCAAATTGTTTCTGCCTGCAACAGGTTGAACCTGGAACGGTTCAAGATCTTTTTTCAAACAACTTGTTAGGGATGTA
CTGTCCGGTCGCCACAATCG

Product: branched chain amino acid ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MLKSSQFRWGPIGSVAQLPIRQTIFQWSERLPAPLRRYGLLGVGLLIPALMLLTSQPSYLANVAVTVAVYMVLAMGLNIV
VGLAGLLDLGYIAFFAVGAYTYAIGAPHGLSFWLALPVAALLAAGFGVLLGAPTLPLRGDYLAIVTLGFGEIIRISLNNL
DWLTRGPAGISGVPAPVIPWIGAKGFTWLTLYQPLQLYFIALAFVAFVYWMTSRLKQSRVGRAWVAIREDEIAAAAMGVD
TVKLKLLAFATGAALAGMVGVLFAVQLTYVSPDSFTLIESVMVVSMVVLGGMGSVPGVALGALILIVLPEALRGFSEYRL
LLFGAAMILVMLIRPQGLLGERQGVPPRPTGAGLEDETPLEVEKRDLPV

Sequences:

>Translated_369_residues
MLKSSQFRWGPIGSVAQLPIRQTIFQWSERLPAPLRRYGLLGVGLLIPALMLLTSQPSYLANVAVTVAVYMVLAMGLNIV
VGLAGLLDLGYIAFFAVGAYTYAIGAPHGLSFWLALPVAALLAAGFGVLLGAPTLPLRGDYLAIVTLGFGEIIRISLNNL
DWLTRGPAGISGVPAPVIPWIGAKGFTWLTLYQPLQLYFIALAFVAFVYWMTSRLKQSRVGRAWVAIREDEIAAAAMGVD
TVKLKLLAFATGAALAGMVGVLFAVQLTYVSPDSFTLIESVMVVSMVVLGGMGSVPGVALGALILIVLPEALRGFSEYRL
LLFGAAMILVMLIRPQGLLGERQGVPPRPTGAGLEDETPLEVEKRDLPV
>Mature_369_residues
MLKSSQFRWGPIGSVAQLPIRQTIFQWSERLPAPLRRYGLLGVGLLIPALMLLTSQPSYLANVAVTVAVYMVLAMGLNIV
VGLAGLLDLGYIAFFAVGAYTYAIGAPHGLSFWLALPVAALLAAGFGVLLGAPTLPLRGDYLAIVTLGFGEIIRISLNNL
DWLTRGPAGISGVPAPVIPWIGAKGFTWLTLYQPLQLYFIALAFVAFVYWMTSRLKQSRVGRAWVAIREDEIAAAAMGVD
TVKLKLLAFATGAALAGMVGVLFAVQLTYVSPDSFTLIESVMVVSMVVLGGMGSVPGVALGALILIVLPEALRGFSEYRL
LLFGAAMILVMLIRPQGLLGERQGVPPRPTGAGLEDETPLEVEKRDLPV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=294, Percent_Identity=46.9387755102041, Blast_Score=230, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39348; Mature: 39348

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSSQFRWGPIGSVAQLPIRQTIFQWSERLPAPLRRYGLLGVGLLIPALMLLTSQPSYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ANVAVTVAVYMVLAMGLNIVVGLAGLLDLGYIAFFAVGAYTYAIGAPHGLSFWLALPVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLAAGFGVLLGAPTLPLRGDYLAIVTLGFGEIIRISLNNLDWLTRGPAGISGVPAPVIPW
HHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCC
IGAKGFTWLTLYQPLQLYFIALAFVAFVYWMTSRLKQSRVGRAWVAIREDEIAAAAMGVD
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCHH
TVKLKLLAFATGAALAGMVGVLFAVQLTYVSPDSFTLIESVMVVSMVVLGGMGSVPGVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
GALILIVLPEALRGFSEYRLLLFGAAMILVMLIRPQGLLGERQGVPPRPTGAGLEDETPL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EVEKRDLPV
CCCCCCCCC
>Mature Secondary Structure
MLKSSQFRWGPIGSVAQLPIRQTIFQWSERLPAPLRRYGLLGVGLLIPALMLLTSQPSYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ANVAVTVAVYMVLAMGLNIVVGLAGLLDLGYIAFFAVGAYTYAIGAPHGLSFWLALPVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLAAGFGVLLGAPTLPLRGDYLAIVTLGFGEIIRISLNNLDWLTRGPAGISGVPAPVIPW
HHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCC
IGAKGFTWLTLYQPLQLYFIALAFVAFVYWMTSRLKQSRVGRAWVAIREDEIAAAAMGVD
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCHH
TVKLKLLAFATGAALAGMVGVLFAVQLTYVSPDSFTLIESVMVVSMVVLGGMGSVPGVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
GALILIVLPEALRGFSEYRLLLFGAAMILVMLIRPQGLLGERQGVPPRPTGAGLEDETPL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EVEKRDLPV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]