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The map label for this gene is sigF [H]

Identifier: 37522237

GI number: 37522237

Start: 2830221

End: 2830985

Strand: Direct

Name: sigF [H]

Synonym: glr2668

Alternate gene names: 37522237

Gene position: 2830221-2830985 (Clockwise)

Preceding gene: 37522232

Following gene: 37522238

Centisome position: 60.75

GC content: 69.54

Gene sequence:

>765_bases
ATGACAGGCACCCCGAAATTTGCCGGGCCGACCCGGCTGTTGTTGCGCGCCTACGGCGAATCTCCTTCCACCGCACTGCG
CAACGAACTGGTCTGCCGCAACCTGGGCCTGGTGCGCAAGATCGCCCACCAACTGGCCCGCGGCAGCAGCGAGCCGTTGG
AAGACCTCGTGCAGGTGGGCGCCATCGGCCTGATGCGCGCCGTCGAGCGCTTCGACCCGTCTTTGGGCCACGCCTTCAGC
TCCTTTGCCACCCCCTGCATCCGGGGAGCAATGCAGCACTACCTGCGCGACCACGGCCACAGCGTGCGCCTGCCGCGCCG
ATTGCTTGAGCTGGATAGTCGCGCCCGCCGGGCGGCCCACGCCCTCAGCCAGCGGCACGGATGCACCCCCAGCGAGCGGC
AGGTGGCTTCGGCCCTCGATATCAGCGCCGAGGAGTGGCTCCAGATGCGCCTGGCGCGCGCCAACCGCACCCTCGTAAGC
CTCGATGCGCCCGCCGGCCCCCACCCCGACACCCCGCCCTTGGTCGAGCTGCTGCCGGGGCCGGACACACCGGAAGTCTC
ACCGGAAGGACAGGAGCGCGCCCACCTCGCCCGTGCCCTCGCCCAACTGGAAGCGGCGACCCAGGTCGTGGTCAGGCAGG
TCTATTTTGAGAAGCGCACCCAGAGCGAGACCGCCCGCCGCCTGGGGGTCTCGCCCATGACCGTCTCGCGCCGTCTCAAA
AAAGGCACCGGCCAGCTGCATACGCTGTTGCAACGCGCCCTGTAG

Upstream 100 bases:

>100_bases
GCCGAACAAACCGGGCCGCCCTGGAGGCGCAATCGACCCGGCCGGGCCGGGCACCCCGGTAGGATGCGGTCCACGACCAC
CTTGATAACCCAAGGCTTTT

Downstream 100 bases:

>100_bases
CATTCTAGGATTCGTTTACATTTCGTTAGCATCAAGCCCGATCCTGTGGGCAATCGCTTTGCCGGATCGGGTGTATTGAC
GCGCTAAGCACAATTGCTTA

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTGTPKFAGPTRLLLRAYGESPSTALRNELVCRNLGLVRKIAHQLARGSSEPLEDLVQVGAIGLMRAVERFDPSLGHAFS
SFATPCIRGAMQHYLRDHGHSVRLPRRLLELDSRARRAAHALSQRHGCTPSERQVASALDISAEEWLQMRLARANRTLVS
LDAPAGPHPDTPPLVELLPGPDTPEVSPEGQERAHLARALAQLEAATQVVVRQVYFEKRTQSETARRLGVSPMTVSRRLK
KGTGQLHTLLQRAL

Sequences:

>Translated_254_residues
MTGTPKFAGPTRLLLRAYGESPSTALRNELVCRNLGLVRKIAHQLARGSSEPLEDLVQVGAIGLMRAVERFDPSLGHAFS
SFATPCIRGAMQHYLRDHGHSVRLPRRLLELDSRARRAAHALSQRHGCTPSERQVASALDISAEEWLQMRLARANRTLVS
LDAPAGPHPDTPPLVELLPGPDTPEVSPEGQERAHLARALAQLEAATQVVVRQVYFEKRTQSETARRLGVSPMTVSRRLK
KGTGQLHTLLQRAL
>Mature_253_residues
TGTPKFAGPTRLLLRAYGESPSTALRNELVCRNLGLVRKIAHQLARGSSEPLEDLVQVGAIGLMRAVERFDPSLGHAFSS
FATPCIRGAMQHYLRDHGHSVRLPRRLLELDSRARRAAHALSQRHGCTPSERQVASALDISAEEWLQMRLARANRTLVSL
DAPAGPHPDTPPLVELLPGPDTPEVSPEGQERAHLARALAQLEAATQVVVRQVYFEKRTQSETARRLGVSPMTVSRRLKK
GTGQLHTLLQRAL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=239, Percent_Identity=28.8702928870293, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788231, Length=130, Percent_Identity=39.2307692307692, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789448, Length=155, Percent_Identity=28.3870967741935, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27951; Mature: 27819

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTPKFAGPTRLLLRAYGESPSTALRNELVCRNLGLVRKIAHQLARGSSEPLEDLVQVG
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AIGLMRAVERFDPSLGHAFSSFATPCIRGAMQHYLRDHGHSVRLPRRLLELDSRARRAAH
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALSQRHGCTPSERQVASALDISAEEWLQMRLARANRTLVSLDAPAGPHPDTPPLVELLPG
HHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHCCCC
PDTPEVSPEGQERAHLARALAQLEAATQVVVRQVYFEKRTQSETARRLGVSPMTVSRRLK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KGTGQLHTLLQRAL
HCHHHHHHHHHHHC
>Mature Secondary Structure 
TGTPKFAGPTRLLLRAYGESPSTALRNELVCRNLGLVRKIAHQLARGSSEPLEDLVQVG
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AIGLMRAVERFDPSLGHAFSSFATPCIRGAMQHYLRDHGHSVRLPRRLLELDSRARRAAH
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALSQRHGCTPSERQVASALDISAEEWLQMRLARANRTLVSLDAPAGPHPDTPPLVELLPG
HHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHCCCC
PDTPEVSPEGQERAHLARALAQLEAATQVVVRQVYFEKRTQSETARRLGVSPMTVSRRLK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KGTGQLHTLLQRAL
HCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]