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The map label for this gene is ilvE [H]

Identifier: 37522151

GI number: 37522151

Start: 2735202

End: 2736077

Strand: Reverse

Name: ilvE [H]

Synonym: gvip358

Alternate gene names: 37522151

Gene position: 2736077-2735202 (Counterclockwise)

Preceding gene: 37522152

Following gene: 37522150

Centisome position: 58.73

GC content: 61.42

Gene sequence:

>876_bases
ATGGGTCTTCTGGTGAACTTCAACGGCACCATCGCGCCGGAAGCCAGAGTTTCGGTCCTCGACCGCGGTTTTCTCTACGG
CGACGGTGTCTACGAGGTCGTGCGCACGATCGGGGGAAGACCCTTCGCTTTGCAGGAGCACCTCGACCGGCTGCGTGCCT
CCGCGAACTATCTCTATCTCGACATTCCCTGGAGCGACGCGCAGATTGCCGCCGAAGTCGAGCGCACCCTGGCCGCCGCC
GGCAGCGGCGACTTTTACATTCGTATCGTCGTCAGCCGCGGCGCGGAAGAAGAAATCAGCCTGCTGCCCGGGCCGCAACT
TGTCCCCGATCTGGTGATCATCGTGCGGTCCATTTCTTCCCAACTGGCTTTGAACGAGCAGGGGGTGCACCTGGCAGTGC
TGCCCCGCTTGCGCAACGACGCGCGGGCGCTCTCTCCAGCCGCCAAGACCGGCAATTATCTGAACAACATCTTGGCGCTC
ATCGAAGCGCGCCGCCTGGGGGCCGACGACGCCCTGCTGCTCAACAGCCGCGGCGAGGTGACCGAGGCCACCACCAGCAA
TCTCTGGTTGGCCAAAGGCGGCACAGTTTATACCCCTGCTGTCGAAGCGGGCATCCTGCACGGCATCACCCGGCATTTTT
TGCTGAAGTTGCTGGGTGATTGGCAGATTCCTTGCATCGAGACTGCACTTACCAAGACAGATTTGTTCGCTGTCGAAGAA
GCCTTTTTGAGTTCGTCGGTGCGTCTGATCGCTCCGGTGCGCCGCATCGATGATTATGAACTGCCCTACTGTCCGGGGGC
GCTTACCCGCCGACTGTTCGATGGGTTGGTCGTGCTGATGCAAGATGCCGTCGCAGCCCCGAAGGTGCCAGTGTAG

Upstream 100 bases:

>100_bases
CTGCAGTCAGTGCGGCACCACCTTTAACCCCTGACTTGGTGGTGCCACAGCCCGGTTCGCTTGCCGGTACACTATGGTGA
GCGCAGTGGGAGTGCAAGCT

Downstream 100 bases:

>100_bases
TACCGCCTCTAAAATGGAGTGCGCCGATTGAGGCAGACGAGTGGCTCTCAGCAAAGATCCCGGCCCGATGAGTCCCGAGC
AATTCTTGCGATGGGAGCGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGLLVNFNGTIAPEARVSVLDRGFLYGDGVYEVVRTIGGRPFALQEHLDRLRASANYLYLDIPWSDAQIAAEVERTLAAA
GSGDFYIRIVVSRGAEEEISLLPGPQLVPDLVIIVRSISSQLALNEQGVHLAVLPRLRNDARALSPAAKTGNYLNNILAL
IEARRLGADDALLLNSRGEVTEATTSNLWLAKGGTVYTPAVEAGILHGITRHFLLKLLGDWQIPCIETALTKTDLFAVEE
AFLSSSVRLIAPVRRIDDYELPYCPGALTRRLFDGLVVLMQDAVAAPKVPV

Sequences:

>Translated_291_residues
MGLLVNFNGTIAPEARVSVLDRGFLYGDGVYEVVRTIGGRPFALQEHLDRLRASANYLYLDIPWSDAQIAAEVERTLAAA
GSGDFYIRIVVSRGAEEEISLLPGPQLVPDLVIIVRSISSQLALNEQGVHLAVLPRLRNDARALSPAAKTGNYLNNILAL
IEARRLGADDALLLNSRGEVTEATTSNLWLAKGGTVYTPAVEAGILHGITRHFLLKLLGDWQIPCIETALTKTDLFAVEE
AFLSSSVRLIAPVRRIDDYELPYCPGALTRRLFDGLVVLMQDAVAAPKVPV
>Mature_290_residues
GLLVNFNGTIAPEARVSVLDRGFLYGDGVYEVVRTIGGRPFALQEHLDRLRASANYLYLDIPWSDAQIAAEVERTLAAAG
SGDFYIRIVVSRGAEEEISLLPGPQLVPDLVIIVRSISSQLALNEQGVHLAVLPRLRNDARALSPAAKTGNYLNNILALI
EARRLGADDALLLNSRGEVTEATTSNLWLAKGGTVYTPAVEAGILHGITRHFLLKLLGDWQIPCIETALTKTDLFAVEEA
FLSSSVRLIAPVRRIDDYELPYCPGALTRRLFDGLVVLMQDAVAAPKVPV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=277, Percent_Identity=27.7978339350181, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787338, Length=249, Percent_Identity=27.710843373494, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 31623; Mature: 31492

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLVNFNGTIAPEARVSVLDRGFLYGDGVYEVVRTIGGRPFALQEHLDRLRASANYLYL
CCEEEECCCCCCCHHHHHHHHCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEE
DIPWSDAQIAAEVERTLAAAGSGDFYIRIVVSRGAEEEISLLPGPQLVPDLVIIVRSISS
EECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEECCCCCHHHHHHHHHHHHHH
QLALNEQGVHLAVLPRLRNDARALSPAAKTGNYLNNILALIEARRLGADDALLLNSRGEV
HHHCCCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCE
TEATTSNLWLAKGGTVYTPAVEAGILHGITRHFLLKLLGDWQIPCIETALTKTDLFAVEE
EECCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AFLSSSVRLIAPVRRIDDYELPYCPGALTRRLFDGLVVLMQDAVAAPKVPV
HHHHCCHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLLVNFNGTIAPEARVSVLDRGFLYGDGVYEVVRTIGGRPFALQEHLDRLRASANYLYL
CEEEECCCCCCCHHHHHHHHCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEE
DIPWSDAQIAAEVERTLAAAGSGDFYIRIVVSRGAEEEISLLPGPQLVPDLVIIVRSISS
EECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHEECCCCCHHHHHHHHHHHHHH
QLALNEQGVHLAVLPRLRNDARALSPAAKTGNYLNNILALIEARRLGADDALLLNSRGEV
HHHCCCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCE
TEATTSNLWLAKGGTVYTPAVEAGILHGITRHFLLKLLGDWQIPCIETALTKTDLFAVEE
EECCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AFLSSSVRLIAPVRRIDDYELPYCPGALTRRLFDGLVVLMQDAVAAPKVPV
HHHHCCHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]