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The map label for this gene is metK

Identifier: 37522146

GI number: 37522146

Start: 2729385

End: 2730644

Strand: Reverse

Name: metK

Synonym: gll2577

Alternate gene names: 37522146

Gene position: 2730644-2729385 (Counterclockwise)

Preceding gene: 37522147

Following gene: 37522145

Centisome position: 58.61

GC content: 64.84

Gene sequence:

>1260_bases
TTGTCACGCTACTTGTTTTCCTCAGAGTCGGTCACTGAAGGTCATCCGGACAAGATCTGCGATCAGATCTCCGATACCAT
CCTCGATGCGCTGTTGACCCAAGATCCCCGTTCGCGGGTGGCGGCCGAGGTGGTCGTCAACACCGGCATGGTGGTGGTCA
CCGGCGAAATCACCACCACGGCCAACGTCAATTTCACCAAACTGGTACGCGACAAAATCCGTGAAATCGGCTACACGGAG
GCCGACAACGGCTTTAGCGCCGATTCGTGCGCGGTGTTTCTAGCCCTTGACGAGCAGTCGCCGGAGATTGCCCAGGGGGT
CAGCTGTGCCCTGGAGGTGCGCACCAGCGAGGAGGATGCCCTCGACCGTATCGGGGCGGGGGATCAAGGCTTGATGTTCG
GCTTTGCCTGCACCGAGACGCCGGAGTTGATGCCGCTGCCCATCAGCGTCGCCCACCGGCTCACCCGCCGCCTCGCCCAG
GTGCGCAAGGACGGTACCCTTGCCTACTTGAAACCGGACGGCAAAGCCCAGGTGACCGTCGAATACGAGCGCAAGGACGG
TGTCGATCGGCCCGGGCGCATCGACACGATCTTGATCTCGACCCAGCACGCCGCTGTGATCGACGATCTCAGCGACAACG
ACGCGGTGCAGGCGCGCATCAAAGCCGACCTGCAAACCCACGTGATCGGCCCGGTCTTCGCGGATCTTGACATCCGCCCC
GACGCCCAGACCCGCTTGTTGGTCAATCCCTCCGGCCGGTTTGTAATCGGCGGTCCGCAGGGAGACTCGGGGCTCACCGG
CCGCAAGATCATCGTGGATACCTACGGCGGCTATGCCCGCCACGGCGGCGGCGCCTTCTCCGGCAAAGATCCCACCAAGG
TGGACCGCTCCGCCGCCTACGCGGCGCGCTACGTCGCCAAAAACATCGTGGCGGCGGAACTGGCCGACCGCTGCGAAGTG
CAGGTGGCTTATGCCATCGGGGTTGCCCGACCGGTGAGCATCTTTGTGGAGACCTTCGGCACCGGCCGCGTCTCGGACGA
GGCTTTGATGCTTCTGGTGCGCGAGCACTTCGATTTGCGCCCGGCGGCCATCCTCCGCGACTTCGACCTGTGCCGCCTGC
CCGCCCAGCGCGGTGGCCGCTTCTACCAGGACGTGGCTGCCTACGGCCACCTCGGTCGCCCCGACCTCGACTTGCCCTGG
GAGCACACCGACAAAGCGGCGACCCTCAAGCAGGCGATCCAGACCGCGGCGGCGGTGTAG

Upstream 100 bases:

>100_bases
CTGCGGTTCCTCACGAACCGGAAGATAAGTCAGGCATCCTGATTCTGCAATGATTGCAAGCCAAGAGACGCATCTACCCA
TTTCATTCGGGAGGGGTTTC

Downstream 100 bases:

>100_bases
CTTCCGGTTTTATCTCGATGGCCGCAAATGATTCAATTTGCGGCCATTTCTCGTTTGCTAAGGTATGGTCATGTATCGTC
TGCAAGCAAGAGACACCAGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSRYLFSSESVTEGHPDKICDQISDTILDALLTQDPRSRVAAEVVVNTGMVVVTGEITTTANVNFTKLVRDKIREIGYTE
ADNGFSADSCAVFLALDEQSPEIAQGVSCALEVRTSEEDALDRIGAGDQGLMFGFACTETPELMPLPISVAHRLTRRLAQ
VRKDGTLAYLKPDGKAQVTVEYERKDGVDRPGRIDTILISTQHAAVIDDLSDNDAVQARIKADLQTHVIGPVFADLDIRP
DAQTRLLVNPSGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAELADRCEV
QVAYAIGVARPVSIFVETFGTGRVSDEALMLLVREHFDLRPAAILRDFDLCRLPAQRGGRFYQDVAAYGHLGRPDLDLPW
EHTDKAATLKQAIQTAAAV

Sequences:

>Translated_419_residues
MSRYLFSSESVTEGHPDKICDQISDTILDALLTQDPRSRVAAEVVVNTGMVVVTGEITTTANVNFTKLVRDKIREIGYTE
ADNGFSADSCAVFLALDEQSPEIAQGVSCALEVRTSEEDALDRIGAGDQGLMFGFACTETPELMPLPISVAHRLTRRLAQ
VRKDGTLAYLKPDGKAQVTVEYERKDGVDRPGRIDTILISTQHAAVIDDLSDNDAVQARIKADLQTHVIGPVFADLDIRP
DAQTRLLVNPSGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAELADRCEV
QVAYAIGVARPVSIFVETFGTGRVSDEALMLLVREHFDLRPAAILRDFDLCRLPAQRGGRFYQDVAAYGHLGRPDLDLPW
EHTDKAATLKQAIQTAAAV
>Mature_418_residues
SRYLFSSESVTEGHPDKICDQISDTILDALLTQDPRSRVAAEVVVNTGMVVVTGEITTTANVNFTKLVRDKIREIGYTEA
DNGFSADSCAVFLALDEQSPEIAQGVSCALEVRTSEEDALDRIGAGDQGLMFGFACTETPELMPLPISVAHRLTRRLAQV
RKDGTLAYLKPDGKAQVTVEYERKDGVDRPGRIDTILISTQHAAVIDDLSDNDAVQARIKADLQTHVIGPVFADLDIRPD
AQTRLLVNPSGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAELADRCEVQ
VAYAIGVARPVSIFVETFGTGRVSDEALMLLVREHFDLRPAAILRDFDLCRLPAQRGGRFYQDVAAYGHLGRPDLDLPWE
HTDKAATLKQAIQTAAAV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=398, Percent_Identity=52.2613065326633, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI4557737, Length=398, Percent_Identity=52.2613065326633, Blast_Score=371, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=412, Percent_Identity=57.2815533980583, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17538498, Length=402, Percent_Identity=50.2487562189055, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538494, Length=401, Percent_Identity=49.6259351620948, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145633, Length=404, Percent_Identity=49.009900990099, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551082, Length=400, Percent_Identity=48.5, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565905, Length=401, Percent_Identity=45.3865336658354, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=355, Percent_Identity=48.169014084507, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI25145635, Length=356, Percent_Identity=46.9101123595506, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=328, Percent_Identity=48.1707317073171, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=55.421686746988, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323209, Length=408, Percent_Identity=51.2254901960784, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6320710, Length=402, Percent_Identity=50, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=398, Percent_Identity=50, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580529, Length=398, Percent_Identity=50, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580537, Length=398, Percent_Identity=50, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580533, Length=398, Percent_Identity=50, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580535, Length=398, Percent_Identity=50, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580531, Length=398, Percent_Identity=50, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24580527, Length=398, Percent_Identity=48.2412060301507, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24580525, Length=398, Percent_Identity=48.2412060301507, Blast_Score=347, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24580541, Length=399, Percent_Identity=46.3659147869674, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=50, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_GLOVI (Q7NHG0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925523.1
- ProteinModelPortal:   Q7NHG0
- SMR:   Q7NHG0
- GeneID:   2600978
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll2577
- NMPDR:   fig|251221.1.peg.2577
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   GVIO251221:GLL2577-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 45374; Mature: 45243

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYLFSSESVTEGHPDKICDQISDTILDALLTQDPRSRVAAEVVVNTGMVVVTGEITTT
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCEEEEEECEEEE
ANVNFTKLVRDKIREIGYTEADNGFSADSCAVFLALDEQSPEIAQGVSCALEVRTSEEDA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEEEEEECCCHHH
LDRIGAGDQGLMFGFACTETPELMPLPISVAHRLTRRLAQVRKDGTLAYLKPDGKAQVTV
HHHCCCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEE
EYERKDGVDRPGRIDTILISTQHAAVIDDLSDNDAVQARIKADLQTHVIGPVFADLDIRP
EECCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCEEHHHHHCHHHHCCCCEEECCCCCC
DAQTRLLVNPSGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAY
CCCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
AARYVAKNIVAAELADRCEVQVAYAIGVARPVSIFVETFGTGRVSDEALMLLVREHFDLR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHEECCCCCCCCHHHHHHHHHHHCCCC
PAAILRDFDLCRLPAQRGGRFYQDVAAYGHLGRPDLDLPWEHTDKAATLKQAIQTAAAV
HHHHHHCCHHHCCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRYLFSSESVTEGHPDKICDQISDTILDALLTQDPRSRVAAEVVVNTGMVVVTGEITTT
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCEEEEEECEEEE
ANVNFTKLVRDKIREIGYTEADNGFSADSCAVFLALDEQSPEIAQGVSCALEVRTSEEDA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEEEEEECCCHHH
LDRIGAGDQGLMFGFACTETPELMPLPISVAHRLTRRLAQVRKDGTLAYLKPDGKAQVTV
HHHCCCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEE
EYERKDGVDRPGRIDTILISTQHAAVIDDLSDNDAVQARIKADLQTHVIGPVFADLDIRP
EECCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCEEHHHHHCHHHHCCCCEEECCCCCC
DAQTRLLVNPSGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAY
CCCEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
AARYVAKNIVAAELADRCEVQVAYAIGVARPVSIFVETFGTGRVSDEALMLLVREHFDLR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHEECCCCCCCCHHHHHHHHHHHCCCC
PAAILRDFDLCRLPAQRGGRFYQDVAAYGHLGRPDLDLPWEHTDKAATLKQAIQTAAAV
HHHHHHCCHHHCCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292