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The map label for this gene is sigA [H]

Identifier: 37522141

GI number: 37522141

Start: 2723645

End: 2724847

Strand: Direct

Name: sigA [H]

Synonym: gvip355

Alternate gene names: 37522141

Gene position: 2723645-2724847 (Clockwise)

Preceding gene: 37522140

Following gene: 37522142

Centisome position: 58.46

GC content: 61.85

Gene sequence:

>1203_bases
ATGACGCAGGCCAATAACGTTCTGGACGCTCTCCAGCAGCCGGCGATGGAACTGGAGGCTCTGGCTTTGATGGCCGAGGA
GGACGACCCTTACGCAGCCCAGGCGGCCGAAGCCGCCGATGCGGCTGCCGCCCGCCCGGCCCGGCGCCGCAGCCGCGCCC
AGCGTGCGCATTACACCGAAGATTCCATCCGCGTCTACCTGCAGGAAATTGGCCGCATTCGCCTGTTGCGCGCCGACGAA
GAAATCGAGTTGGCCCGCCAGATCGCCGATTTGCTGACCCTGGAGCGCCTGCGCGCCCGCATGGGAGCTGCCGGGACGGA
CATGCACAGCGATGTGGCCGATTTGCTGGGCGACGACGATCTGCTGACCGAGAATCTGCTTGCTTCGGGCGATCAAGTCA
CCGAAAGTGAGCAAGAACTCAGGCGCTGGGCAGAAGCGGCGGGCATTGCCTACGAACAATTTCGCGACCGCATCGCCTAC
GGCCGGCGGGCCAAAGACAAGATGGTCCAGTCCAATTTGCGTCTGGTGGTTTCAATCGCCAAGAAGTACATGAACCGCGG
TCTGTCCTTTCAAGATCTCATCCAGGAAGGTTCGCTGGGACTCATCCGCGCCGCCGAAAAATTCGATCACGAAAAGGGCT
ACAAATTTTCCACCTACGCCACCTGGTGGATCCGCCAGGCGATCACCCGCGCCATCGCCGACCAATCGCGCACCATCCGC
CTACCGGTGCACCTCTACGAGACCATCTCCCGCATCAAGAAGACCACCAAGCTGCTCTCGCAGGAGCTGGGCCGCAAGCC
TACCGAAGAGGAGATCGCCACCCGCATGGAGATGACGATCGAGAAATTGCGCTTCATCGCCAAGTCCGCCCAGTTGCCCA
TCTCCCTGGAGACCCCGATCGGCAAAGAAGAAGATTCCCGGTTGGGCGATTTTATCGAAGCGGACGGCGAGACCCCCGAG
GACAAAGTTGCCAAGAACCTCCTGCGCGAAGATCTTGAGGCGGTGCTCAACACCCTCTCGCCACGCGAGCGCGACGTGCT
CAGGTTGCGCTACGGCCTCGACGACGGCCGCATGAAGACCCTCGAAGAAATTGGCCAGCTGTTCAACGTCACCCGCGAGC
GTATCCGCCAGATCGAGGCGAAGGCGCTGCGCAAGCTGCGCCATCCCAACCGCAACAGCGTCCTCAAGGAGTACATCCGC
TAG

Upstream 100 bases:

>100_bases
AACCTTGCATCCGGTGCGTGCGACGATTTTTCTTCCGAACGGAAGAGTATAAAGGACACGGTTTGTCTGTTCTGGATCCG
CTATTTATGAGGAGTGCAGC

Downstream 100 bases:

>100_bases
GAGTCCATGGGCGCTCGTGCATTCAAACCCGGTTGGACGAGCGTCGCAGCGGCGCTGTCCAGACTGGATCTTGGATTGCT
GGCGATCGGCTACGCGGTGT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTQANNVLDALQQPAMELEALALMAEEDDPYAAQAAEAADAAAARPARRRSRAQRAHYTEDSIRVYLQEIGRIRLLRADE
EIELARQIADLLTLERLRARMGAAGTDMHSDVADLLGDDDLLTENLLASGDQVTESEQELRRWAEAAGIAYEQFRDRIAY
GRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR
LPVHLYETISRIKKTTKLLSQELGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADGETPE
DKVAKNLLREDLEAVLNTLSPRERDVLRLRYGLDDGRMKTLEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR

Sequences:

>Translated_400_residues
MTQANNVLDALQQPAMELEALALMAEEDDPYAAQAAEAADAAAARPARRRSRAQRAHYTEDSIRVYLQEIGRIRLLRADE
EIELARQIADLLTLERLRARMGAAGTDMHSDVADLLGDDDLLTENLLASGDQVTESEQELRRWAEAAGIAYEQFRDRIAY
GRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR
LPVHLYETISRIKKTTKLLSQELGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADGETPE
DKVAKNLLREDLEAVLNTLSPRERDVLRLRYGLDDGRMKTLEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
>Mature_399_residues
TQANNVLDALQQPAMELEALALMAEEDDPYAAQAAEAADAAAARPARRRSRAQRAHYTEDSIRVYLQEIGRIRLLRADEE
IELARQIADLLTLERLRARMGAAGTDMHSDVADLLGDDDLLTENLLASGDQVTESEQELRRWAEAAGIAYEQFRDRIAYG
RRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRL
PVHLYETISRIKKTTKLLSQELGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEADGETPED
KVAKNLLREDLEAVLNTLSPRERDVLRLRYGLDDGRMKTLEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=285, Percent_Identity=54.3859649122807, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1789098, Length=283, Percent_Identity=39.2226148409894, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 45732; Mature: 45601

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQANNVLDALQQPAMELEALALMAEEDDPYAAQAAEAADAAAARPARRRSRAQRAHYTE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCH
DSIRVYLQEIGRIRLLRADEEIELARQIADLLTLERLRARMGAAGTDMHSDVADLLGDDD
HHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
LLTENLLASGDQVTESEQELRRWAEAAGIAYEQFRDRIAYGRRAKDKMVQSNLRLVVSIA
HHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LPVHLYETISRIKKTTKLLSQELGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPI
EHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
GKEEDSRLGDFIEADGETPEDKVAKNLLREDLEAVLNTLSPRERDVLRLRYGLDDGRMKT
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
LEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
>Mature Secondary Structure 
TQANNVLDALQQPAMELEALALMAEEDDPYAAQAAEAADAAAARPARRRSRAQRAHYTE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCH
DSIRVYLQEIGRIRLLRADEEIELARQIADLLTLERLRARMGAAGTDMHSDVADLLGDDD
HHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
LLTENLLASGDQVTESEQELRRWAEAAGIAYEQFRDRIAYGRRAKDKMVQSNLRLVVSIA
HHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIR
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LPVHLYETISRIKKTTKLLSQELGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPI
EHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
GKEEDSRLGDFIEADGETPEDKVAKNLLREDLEAVLNTLSPRERDVLRLRYGLDDGRMKT
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
LEEIGQLFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]