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The map label for this gene is cobJ [H]

Identifier: 37522115

GI number: 37522115

Start: 2699244

End: 2701010

Strand: Reverse

Name: cobJ [H]

Synonym: gvip349

Alternate gene names: 37522115

Gene position: 2701010-2699244 (Counterclockwise)

Preceding gene: 37522118

Following gene: 37522113

Centisome position: 57.97

GC content: 69.89

Gene sequence:

>1767_bases
GTGACTCAGCCCAAACCTGTGTATCTGATTGCTCCCACGACGGCGGGGCGGAAACTGGCCCAGGCCCTGCAGGCGCTGTT
GCCCGAAGCAATGCTCTGGAAGCGACCGCTCGGCGAACTGGAAAGCGCCTGGCGCGAGGCGGGGCAACTGGTCTTTGTGC
TGGCGGTCGGGGCAGTGGTGCGGCTGATTGCGCCCCTGCTCGCCGACAAGCGCACCGATCCGGGCGTGGTGGTTATTGAC
GAGGCGGGACGCTTTGCTGTGAGCGTGAGCGCGGGCCACATCGGGGGCGCGGACGCCCTGGCCGAGCGAATCGCCGCACT
GCTGAGCGCCACGGCCGTGCTCACCGGCGCCGGCGGCCGCCACGGCCTGCCGCCGGTCGACTTGTTGGGCAAAGCTTATG
GCTGGCAAGCCGGGGAAGCTTCCGACTGGAACCGCGTAGCGGCCCTGGTGGCAGCGGGGGAGCCGGTGCAGGTGGTGCAG
GAGTGCGGCCTCGACCTCTGGCGCGCCGCCCTGCCCGAGAGCCACCCGTTTGTGGATACCGCGGCGGCGGCAGGCAGGCT
GTGGATCAGCCACCGGCTGCCAACCGCCGGCACCCTGCCCACGGTGTGCTGGCATCCGCGGGTGATCTGGCTGGGGGTCG
GCTGCGAGCGGGGGATCGCCGCCGACTTTCTCGAGCAATCGATCCGGCAGGCGCTGGCCGGGGCGGGGCTGGCCTTCGAG
GCGGTGGCGGGGATTGCCAGTCTGGATATCAAAGCCGACGAAGCGGGACTGCTGGCGGTGGCCGAGCGCCACGGCTGGCC
CGTGCGCTTTTTCGACGCCGCCACGCTGGCCCGCTGCCCGGTACCCAACCCTTCGCAGGCCGTGGCGGACTGTGTGGGGA
CTCCCTCGGTGGCCGAGGCTGCGGCCCTGTTGGCGGGCGAGGCTTTGCAACTGGCGGCCCCCAAGCGCGTATTTAAGGCG
GCCGGAGCGGGCGCCTGCACGGTAGCCGCCGCCGTTGCCATTCAGGAATTCAACCCGGCGCCGGGCAGACTGCTGCTGAT
TGGCAGCGGCCCAGGAGCGCTCGATCAGATTACTCCCGCTGCCCGCGCCGCCCTGGGAGCGGCCCAGGTGGTGATTGGCT
ATCAACTCTATATCGATCTGATTCGGCCGCTGCTTGCACCCACCCAGATCGTCGAGACCAGCCCAATTACCCAGGAGGTG
GCGCGCGCCGAGCGGGCTATCGCCCTGGCGAGGCGAGGTCTGGCGGTGGCGGTCATCTCCAGCGGCGATGCGGGTATCTA
CGGCATGGCCGGGCTGGTGTTTGAGCGGCTGGCCCGGGGCGGTTGGAACGGCCAAGCTCCCGCCGTGGATGTCCTGCCCG
GAATTACCGCCCTGCAAGCCGCCGCCGCCCGCGTCGGCGCCCCGCTGATGCACGACTTTTGCGCCATCAGCCTCTCGGAC
TTGCTCACGCCCTGGCAGACGATCTGCACCCGGCTTGAAGCCGCCGCCCGGGCCGATTTTGTCGTCGCCCTCTACAACCC
CCGCTCCGCTTCGCGCACCCAACAAATTGCCCAGGCGCGCGAAATCCTGCTCGCCCACCGCGATCCGGCTACCCCCGTCG
CTCTGGTGCGCTGCGCCTACCGCCCCGACGAGCAGGTCAGCCTGCACACCCTGGCTGATCTCCCTTTGGAATCCGTCGAC
ATGCTTACCACGGTCCTCATCGGCAACCGCGCCACCTTCCGCCGGGGCGACCGGCTGATCACCCCCCGGGGCTACCCGCA
CCCCTAG

Upstream 100 bases:

>100_bases
GTAGACGCAATGCCCGTCAAGCCCCGAGACAGGCAGCCTGGCCGAAAGGTGCCCCGCAGCGGCTGCGTGCTAGAATGCAC
CGCCGAGAAACAAGGCGCCT

Downstream 100 bases:

>100_bases
CGGCGCTCCCCGCCGCTCAGTAGACGAGAATTGCTATTGCTTTTGTTTATCGTGCAGAACATGATAAAAATTGCACAAAC
TCGGACAAAGGAAAAATCCC

Product: precorrin methylase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MTQPKPVYLIAPTTAGRKLAQALQALLPEAMLWKRPLGELESAWREAGQLVFVLAVGAVVRLIAPLLADKRTDPGVVVID
EAGRFAVSVSAGHIGGADALAERIAALLSATAVLTGAGGRHGLPPVDLLGKAYGWQAGEASDWNRVAALVAAGEPVQVVQ
ECGLDLWRAALPESHPFVDTAAAAGRLWISHRLPTAGTLPTVCWHPRVIWLGVGCERGIAADFLEQSIRQALAGAGLAFE
AVAGIASLDIKADEAGLLAVAERHGWPVRFFDAATLARCPVPNPSQAVADCVGTPSVAEAAALLAGEALQLAAPKRVFKA
AGAGACTVAAAVAIQEFNPAPGRLLLIGSGPGALDQITPAARAALGAAQVVIGYQLYIDLIRPLLAPTQIVETSPITQEV
ARAERAIALARRGLAVAVISSGDAGIYGMAGLVFERLARGGWNGQAPAVDVLPGITALQAAAARVGAPLMHDFCAISLSD
LLTPWQTICTRLEAAARADFVVALYNPRSASRTQQIAQAREILLAHRDPATPVALVRCAYRPDEQVSLHTLADLPLESVD
MLTTVLIGNRATFRRGDRLITPRGYPHP

Sequences:

>Translated_588_residues
MTQPKPVYLIAPTTAGRKLAQALQALLPEAMLWKRPLGELESAWREAGQLVFVLAVGAVVRLIAPLLADKRTDPGVVVID
EAGRFAVSVSAGHIGGADALAERIAALLSATAVLTGAGGRHGLPPVDLLGKAYGWQAGEASDWNRVAALVAAGEPVQVVQ
ECGLDLWRAALPESHPFVDTAAAAGRLWISHRLPTAGTLPTVCWHPRVIWLGVGCERGIAADFLEQSIRQALAGAGLAFE
AVAGIASLDIKADEAGLLAVAERHGWPVRFFDAATLARCPVPNPSQAVADCVGTPSVAEAAALLAGEALQLAAPKRVFKA
AGAGACTVAAAVAIQEFNPAPGRLLLIGSGPGALDQITPAARAALGAAQVVIGYQLYIDLIRPLLAPTQIVETSPITQEV
ARAERAIALARRGLAVAVISSGDAGIYGMAGLVFERLARGGWNGQAPAVDVLPGITALQAAAARVGAPLMHDFCAISLSD
LLTPWQTICTRLEAAARADFVVALYNPRSASRTQQIAQAREILLAHRDPATPVALVRCAYRPDEQVSLHTLADLPLESVD
MLTTVLIGNRATFRRGDRLITPRGYPHP
>Mature_587_residues
TQPKPVYLIAPTTAGRKLAQALQALLPEAMLWKRPLGELESAWREAGQLVFVLAVGAVVRLIAPLLADKRTDPGVVVIDE
AGRFAVSVSAGHIGGADALAERIAALLSATAVLTGAGGRHGLPPVDLLGKAYGWQAGEASDWNRVAALVAAGEPVQVVQE
CGLDLWRAALPESHPFVDTAAAAGRLWISHRLPTAGTLPTVCWHPRVIWLGVGCERGIAADFLEQSIRQALAGAGLAFEA
VAGIASLDIKADEAGLLAVAERHGWPVRFFDAATLARCPVPNPSQAVADCVGTPSVAEAAALLAGEALQLAAPKRVFKAA
GAGACTVAAAVAIQEFNPAPGRLLLIGSGPGALDQITPAARAALGAAQVVIGYQLYIDLIRPLLAPTQIVETSPITQEVA
RAERAIALARRGLAVAVISSGDAGIYGMAGLVFERLARGGWNGQAPAVDVLPGITALQAAAARVGAPLMHDFCAISLSDL
LTPWQTICTRLEAAARADFVVALYNPRSASRTQQIAQAREILLAHRDPATPVALVRCAYRPDEQVSLHTLADLPLESVDM
LTTVLIGNRATFRRGDRLITPRGYPHP

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 61367; Mature: 61235

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPKPVYLIAPTTAGRKLAQALQALLPEAMLWKRPLGELESAWREAGQLVFVLAVGAVV
CCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCEEHHHHHHHHH
RLIAPLLADKRTDPGVVVIDEAGRFAVSVSAGHIGGADALAERIAALLSATAVLTGAGGR
HHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
HGLPPVDLLGKAYGWQAGEASDWNRVAALVAAGEPVQVVQECGLDLWRAALPESHPFVDT
CCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHH
AAAAGRLWISHRLPTAGTLPTVCWHPRVIWLGVGCERGIAADFLEQSIRQALAGAGLAFE
HHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
AVAGIASLDIKADEAGLLAVAERHGWPVRFFDAATLARCPVPNPSQAVADCVGTPSVAEA
HHHHHHHEECCCCCCCEEEEHHCCCCCEEEECCHHHHCCCCCCCHHHHHHHCCCCHHHHH
AALLAGEALQLAAPKRVFKAAGAGACTVAAAVAIQEFNPAPGRLLLIGSGPGALDQITPA
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCHH
ARAALGAAQVVIGYQLYIDLIRPLLAPTQIVETSPITQEVARAERAIALARRGLAVAVIS
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
SGDAGIYGMAGLVFERLARGGWNGQAPAVDVLPGITALQAAAARVGAPLMHDFCAISLSD
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LLTPWQTICTRLEAAARADFVVALYNPRSASRTQQIAQAREILLAHRDPATPVALVRCAY
HCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
RPDEQVSLHTLADLPLESVDMLTTVLIGNRATFRRGDRLITPRGYPHP
CCCCCEEHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCC
>Mature Secondary Structure 
TQPKPVYLIAPTTAGRKLAQALQALLPEAMLWKRPLGELESAWREAGQLVFVLAVGAVV
CCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCEEHHHHHHHHH
RLIAPLLADKRTDPGVVVIDEAGRFAVSVSAGHIGGADALAERIAALLSATAVLTGAGGR
HHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
HGLPPVDLLGKAYGWQAGEASDWNRVAALVAAGEPVQVVQECGLDLWRAALPESHPFVDT
CCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHH
AAAAGRLWISHRLPTAGTLPTVCWHPRVIWLGVGCERGIAADFLEQSIRQALAGAGLAFE
HHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHH
AVAGIASLDIKADEAGLLAVAERHGWPVRFFDAATLARCPVPNPSQAVADCVGTPSVAEA
HHHHHHHEECCCCCCCEEEEHHCCCCCEEEECCHHHHCCCCCCCHHHHHHHCCCCHHHHH
AALLAGEALQLAAPKRVFKAAGAGACTVAAAVAIQEFNPAPGRLLLIGSGPGALDQITPA
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCHH
ARAALGAAQVVIGYQLYIDLIRPLLAPTQIVETSPITQEVARAERAIALARRGLAVAVIS
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
SGDAGIYGMAGLVFERLARGGWNGQAPAVDVLPGITALQAAAARVGAPLMHDFCAISLSD
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LLTPWQTICTRLEAAARADFVVALYNPRSASRTQQIAQAREILLAHRDPATPVALVRCAY
HCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
RPDEQVSLHTLADLPLESVDMLTTVLIGNRATFRRGDRLITPRGYPHP
CCCCCEEHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]