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The map label for this gene is rihA [H]

Identifier: 37522048

GI number: 37522048

Start: 2636899

End: 2637810

Strand: Reverse

Name: rihA [H]

Synonym: gll2479

Alternate gene names: 37522048

Gene position: 2637810-2636899 (Counterclockwise)

Preceding gene: 37522051

Following gene: 37522046

Centisome position: 56.62

GC content: 68.2

Gene sequence:

>912_bases
TTGCGAAAGCCGGTCATCCACGACCAAGACGGTCACGCCGACGATCTGGTCGCCACATTGCTGTTGGCGCTCTACCCGGC
TGTGGATCTGAGGGCCGTCGTCGTCAACAGCGGAGACTGTATCCCCCAGGTTTCCGCCGCCGTTCTGGCGGCCCTGCTCG
ATCGCCTGGGAAAAGGCGCGGTGCCGGTGGCCTGGCAGCACCATGCCCTGCCCCATCCCTTTCCCACCGCCTGGCAGCGC
GAGAGCCTCAACTATCTGCGGGCCTTTCCGGAAGCCGAACGGTACTACCCGGCCGCCGCCGGCACGCGGCTGCTCACCGA
GACGATCCTGGCGGCGGGCGAACCGGTGACTTTGCTGGTCACCGGCCCCTGCACGAATCTGGCGGCGGCTCTGCGCGCCG
CTCCCGAGATTGCCCGGCACCTGGAGCGGGTGGTGTTGATGGCCGGGGGCCTGGAGGCGGGCGGCAACGTCGCAGGCGAG
CAAGACCATGACGGCAGCGCCGAGTGGAATCTTTACTGCGACCCTCCTGCCGCCGCCTACTGCCTGCAGGCCGGGCTCGC
CCTGGACTTTGTCAGCCTCGATGTCACCAATTTTGTACCGATGACCGCCCGCTTCGTGGAGACACTGCGCTCGCGCGGCG
GCGCCTGCCGCCTTGCCGCCGAACTGCTCGCAGGCGTCGAGCAGCAGAACTATTTTTTCTGGGACACCCTGGCCGCTCTG
CTGACCTGCGAGCCCGACTTTTTGCCCCGCGGGCGCCGGACGCTCACCGTGCGCACCGACCCACCCGCCGCGGGACGGCT
CGAAAAAACCGCCTCGGGTGTACCCGCGCGGGTCTTCGAGCCCGATGCCGACGTGGAAAGCCTGGGAGCGCGGGTGGAGG
TGCGGGTGGCCGAAATTTTGAGCGCCCTCTAG

Upstream 100 bases:

>100_bases
CTCCCTCCACAGCTGTCCATCTGCATCCGACCTTGGAGGGAATTCTGATCCCCGCCTCTGAAGCGGTAGGCTATGGAAGA
TTTGCCCCGGGAGCGGTGCT

Downstream 100 bases:

>100_bases
CCGTAGAGCCGCTTGAAGACGGCGACGGTCCGCTCCACTCCCGCGTCATCGATATCAAGGTGGGTGACCAACCGCAGACG
CCGGGGACCAAAGACACTCA

Product: nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRKPVIHDQDGHADDLVATLLLALYPAVDLRAVVVNSGDCIPQVSAAVLAALLDRLGKGAVPVAWQHHALPHPFPTAWQR
ESLNYLRAFPEAERYYPAAAGTRLLTETILAAGEPVTLLVTGPCTNLAAALRAAPEIARHLERVVLMAGGLEAGGNVAGE
QDHDGSAEWNLYCDPPAAAYCLQAGLALDFVSLDVTNFVPMTARFVETLRSRGGACRLAAELLAGVEQQNYFFWDTLAAL
LTCEPDFLPRGRRTLTVRTDPPAAGRLEKTASGVPARVFEPDADVESLGARVEVRVAEILSAL

Sequences:

>Translated_303_residues
MRKPVIHDQDGHADDLVATLLLALYPAVDLRAVVVNSGDCIPQVSAAVLAALLDRLGKGAVPVAWQHHALPHPFPTAWQR
ESLNYLRAFPEAERYYPAAAGTRLLTETILAAGEPVTLLVTGPCTNLAAALRAAPEIARHLERVVLMAGGLEAGGNVAGE
QDHDGSAEWNLYCDPPAAAYCLQAGLALDFVSLDVTNFVPMTARFVETLRSRGGACRLAAELLAGVEQQNYFFWDTLAAL
LTCEPDFLPRGRRTLTVRTDPPAAGRLEKTASGVPARVFEPDADVESLGARVEVRVAEILSAL
>Mature_303_residues
MRKPVIHDQDGHADDLVATLLLALYPAVDLRAVVVNSGDCIPQVSAAVLAALLDRLGKGAVPVAWQHHALPHPFPTAWQR
ESLNYLRAFPEAERYYPAAAGTRLLTETILAAGEPVTLLVTGPCTNLAAALRAAPEIARHLERVVLMAGGLEAGGNVAGE
QDHDGSAEWNLYCDPPAAAYCLQAGLALDFVSLDVTNFVPMTARFVETLRSRGGACRLAAELLAGVEQQNYFFWDTLAAL
LTCEPDFLPRGRRTLTVRTDPPAAGRLEKTASGVPARVFEPDADVESLGARVEVRVAEILSAL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=227, Percent_Identity=28.6343612334802, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788486, Length=233, Percent_Identity=25.3218884120172, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786213, Length=105, Percent_Identity=35.2380952380952, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32432; Mature: 32432

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPVIHDQDGHADDLVATLLLALYPAVDLRAVVVNSGDCIPQVSAAVLAALLDRLGKGA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
VPVAWQHHALPHPFPTAWQRESLNYLRAFPEAERYYPAAAGTRLLTETILAAGEPVTLLV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
TGPCTNLAAALRAAPEIARHLERVVLMAGGLEAGGNVAGEQDHDGSAEWNLYCDPPAAAY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHH
CLQAGLALDFVSLDVTNFVPMTARFVETLRSRGGACRLAAELLAGVEQQNYFFWDTLAAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHCCCHHHHHHHHH
LTCEPDFLPRGRRTLTVRTDPPAAGRLEKTASGVPARVFEPDADVESLGARVEVRVAEIL
HHCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCHHHHCCEEEEHHHHHH
SAL
HCC
>Mature Secondary Structure
MRKPVIHDQDGHADDLVATLLLALYPAVDLRAVVVNSGDCIPQVSAAVLAALLDRLGKGA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
VPVAWQHHALPHPFPTAWQRESLNYLRAFPEAERYYPAAAGTRLLTETILAAGEPVTLLV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEE
TGPCTNLAAALRAAPEIARHLERVVLMAGGLEAGGNVAGEQDHDGSAEWNLYCDPPAAAY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHH
CLQAGLALDFVSLDVTNFVPMTARFVETLRSRGGACRLAAELLAGVEQQNYFFWDTLAAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHCCCHHHHHHHHH
LTCEPDFLPRGRRTLTVRTDPPAAGRLEKTASGVPARVFEPDADVESLGARVEVRVAEIL
HHCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCHHHHCCEEEEHHHHHH
SAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA