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The map label for this gene is trmE

Identifier: 37522021

GI number: 37522021

Start: 2607896

End: 2609257

Strand: Direct

Name: trmE

Synonym: glr2452

Alternate gene names: 37522021

Gene position: 2607896-2609257 (Clockwise)

Preceding gene: 37522020

Following gene: 37522031

Centisome position: 55.98

GC content: 65.2

Gene sequence:

>1362_bases
TTGTCCGACACCATCTGCGCCATCGCCACCGCCATCGTCCCCCAGCAAGGCAGCGTCGGGATCGTGCGTTTGTCGGGCAG
TGAGGCGCTCGCCCTCGCCAAACGGGTCTTTCGATTGCGCGGCAAACAAGCTTTCGAGAGCCACCGCATCCACTACGGCC
ACTTTCTGGACGCCGATGGCGGCATTCTCGACGAGTGCCTGGTGCTATATATGCAAGCGCCGCGCTCCTTCACCCGCGAG
GATGTGGTTGAATTTCACTGCCACGGCGGGATCGCTGTCATCCAGCAGATTTTGCGCCGCTGCGTCGAACTGGGAGCGCG
TCTGGCCCGGCCCGGCGAATTTACCCTGCGGGCCTTTTTGAACGGCCGCATCGACCTTACCGAAGCGGAGGCGGTGGCGG
AACTGGTCTCGGCCCGCTCGCCGGAGGCGGCCCGGCTCGCGGTTGGGGGACTGGCGGGCAAACTGGCGGGTGAAGTGCGC
CAACTGCGATCCGGTTGCCTTGACTTGCTGGCTGAACTGGAAGCTCGGCTGGATTTTGAAGACGACCTTCCGCCGCTCGA
TACCGGGGCGGTGCGCGCCAATATCGGCCAGAGCCTCGCACATGCCCGGAGGCTGATGGCCACCAGCACCCGGGGCGAAC
TGCTGCGCACGGGCCTCAAAGTCGCCATCGTCGGGAGACCCAACGTCGGCAAATCGAGCTTGCTCAACGCCTGGAGCCGC
ACCGACCGGGCGATTGTGACCGATTTACCCGGCACCACCCGCGACGTGGTCGAATCGATGCTGAATGTCCAGGGCATTCC
GGTGCAGGTGCTCGATACGGCCGGTATCCGCGAGGCCACCGACACCGTCGAGCGCATCGGCATCGAGCGCTCCCACGCCG
CCGCCCGGGAGGCGGATCTGGTGCTGCTGGTCATCGATCGCACCGAGGGATGGACCTGTGCCGACAGCGACATTTTTGCC
CAGGTGCGCGAGCGACCGGTGATCGTCGTCGTCAACAAATCGGATTTAGAGGGTCCGCCGGTCTACACCCCGTCGCGGGG
GGTGCTGGCGACAGTGCCCACCGCCGCTCCCGTGGGCGACGGGATCGACGCGCTCGAAGGGGCGATCCTGGCTGCTTTGG
GCCAAGAAGCATTTTCTGTGGCCGACGGCGATTTTGCCGTCAACGAGCGGCAGTATGAAGCGCTCGTGCGCGCCGAAGTG
GCCCTGGTGCGGGTGGTGGAGACCGTGGAGGCGGCTCTGCCCATTGACTTTTGGACCATCGATCTGCGCGCCGCCGCCCA
TGCCCTCGGCGAAGTGACCGGCGAATCGGTCACCGAGGCGGTGCTTGAGCGCATCTTCAGCAAATTCTGCATCGGCAAGT
AA

Upstream 100 bases:

>100_bases
TCTACACCCGCCTCGCCCGCAATTACGCAGTCGGCCGTCCGCGGGCCGACAGTACGCCAAACCATCCCGGATAATCGAAG
CACAACGACCCACAAAACCC

Downstream 100 bases:

>100_bases
ATTCCCCAGTGGCGCTCAACGCTCTGAACTACCCGGAAATTCTGCAACAGTTCTGCCGGTGCGCACGGCCCGATCGAGCA
CCCGCAACGGTGCCACGGCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSDTICAIATAIVPQQGSVGIVRLSGSEALALAKRVFRLRGKQAFESHRIHYGHFLDADGGILDECLVLYMQAPRSFTRE
DVVEFHCHGGIAVIQQILRRCVELGARLARPGEFTLRAFLNGRIDLTEAEAVAELVSARSPEAARLAVGGLAGKLAGEVR
QLRSGCLDLLAELEARLDFEDDLPPLDTGAVRANIGQSLAHARRLMATSTRGELLRTGLKVAIVGRPNVGKSSLLNAWSR
TDRAIVTDLPGTTRDVVESMLNVQGIPVQVLDTAGIREATDTVERIGIERSHAAAREADLVLLVIDRTEGWTCADSDIFA
QVRERPVIVVVNKSDLEGPPVYTPSRGVLATVPTAAPVGDGIDALEGAILAALGQEAFSVADGDFAVNERQYEALVRAEV
ALVRVVETVEAALPIDFWTIDLRAAAHALGEVTGESVTEAVLERIFSKFCIGK

Sequences:

>Translated_453_residues
MSDTICAIATAIVPQQGSVGIVRLSGSEALALAKRVFRLRGKQAFESHRIHYGHFLDADGGILDECLVLYMQAPRSFTRE
DVVEFHCHGGIAVIQQILRRCVELGARLARPGEFTLRAFLNGRIDLTEAEAVAELVSARSPEAARLAVGGLAGKLAGEVR
QLRSGCLDLLAELEARLDFEDDLPPLDTGAVRANIGQSLAHARRLMATSTRGELLRTGLKVAIVGRPNVGKSSLLNAWSR
TDRAIVTDLPGTTRDVVESMLNVQGIPVQVLDTAGIREATDTVERIGIERSHAAAREADLVLLVIDRTEGWTCADSDIFA
QVRERPVIVVVNKSDLEGPPVYTPSRGVLATVPTAAPVGDGIDALEGAILAALGQEAFSVADGDFAVNERQYEALVRAEV
ALVRVVETVEAALPIDFWTIDLRAAAHALGEVTGESVTEAVLERIFSKFCIGK
>Mature_452_residues
SDTICAIATAIVPQQGSVGIVRLSGSEALALAKRVFRLRGKQAFESHRIHYGHFLDADGGILDECLVLYMQAPRSFTRED
VVEFHCHGGIAVIQQILRRCVELGARLARPGEFTLRAFLNGRIDLTEAEAVAELVSARSPEAARLAVGGLAGKLAGEVRQ
LRSGCLDLLAELEARLDFEDDLPPLDTGAVRANIGQSLAHARRLMATSTRGELLRTGLKVAIVGRPNVGKSSLLNAWSRT
DRAIVTDLPGTTRDVVESMLNVQGIPVQVLDTAGIREATDTVERIGIERSHAAAREADLVLLVIDRTEGWTCADSDIFAQ
VRERPVIVVVNKSDLEGPPVYTPSRGVLATVPTAAPVGDGIDALEGAILAALGQEAFSVADGDFAVNERQYEALVRAEVA
LVRVVETVEAALPIDFWTIDLRAAAHALGEVTGESVTEAVLERIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=422, Percent_Identity=35.3080568720379, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI193082993, Length=412, Percent_Identity=35.9223300970874, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI193082988, Length=445, Percent_Identity=33.0337078651685, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=38.4782608695652, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI87082120, Length=150, Percent_Identity=40.6666666666667, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=31.3304721030043, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=31.1608961303462, Blast_Score=229, Evalue=8e-61,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=29.7352342158859, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_GLOVI (Q7NHT3)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925398.1
- ProteinModelPortal:   Q7NHT3
- SMR:   Q7NHT3
- GeneID:   2600853
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr2452
- NMPDR:   fig|251221.1.peg.2452
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   GVIO251221:GLR2452-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48588; Mature: 48457

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 84-84 BINDING 123-123 BINDING 453-453

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTICAIATAIVPQQGSVGIVRLSGSEALALAKRVFRLRGKQAFESHRIHYGHFLDADG
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHCCEEECEEECCCC
GILDECLVLYMQAPRSFTREDVVEFHCHGGIAVIQQILRRCVELGARLARPGEFTLRAFL
CHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NGRIDLTEAEAVAELVSARSPEAARLAVGGLAGKLAGEVRQLRSGCLDLLAELEARLDFE
CCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DDLPPLDTGAVRANIGQSLAHARRLMATSTRGELLRTGLKVAIVGRPNVGKSSLLNAWSR
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
TDRAIVTDLPGTTRDVVESMLNVQGIPVQVLDTAGIREATDTVERIGIERSHAAAREADL
CCCEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHCCCCE
VLLVIDRTEGWTCADSDIFAQVRERPVIVVVNKSDLEGPPVYTPSRGVLATVPTAAPVGD
EEEEEECCCCCEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCC
GIDALEGAILAALGQEAFSVADGDFAVNERQYEALVRAEVALVRVVETVEAALPIDFWTI
CHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
DLRAAAHALGEVTGESVTEAVLERIFSKFCIGK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDTICAIATAIVPQQGSVGIVRLSGSEALALAKRVFRLRGKQAFESHRIHYGHFLDADG
CCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHCCEEECEEECCCC
GILDECLVLYMQAPRSFTREDVVEFHCHGGIAVIQQILRRCVELGARLARPGEFTLRAFL
CHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
NGRIDLTEAEAVAELVSARSPEAARLAVGGLAGKLAGEVRQLRSGCLDLLAELEARLDFE
CCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DDLPPLDTGAVRANIGQSLAHARRLMATSTRGELLRTGLKVAIVGRPNVGKSSLLNAWSR
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
TDRAIVTDLPGTTRDVVESMLNVQGIPVQVLDTAGIREATDTVERIGIERSHAAAREADL
CCCEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHCCCCE
VLLVIDRTEGWTCADSDIFAQVRERPVIVVVNKSDLEGPPVYTPSRGVLATVPTAAPVGD
EEEEEECCCCCEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCC
GIDALEGAILAALGQEAFSVADGDFAVNERQYEALVRAEVALVRVVETVEAALPIDFWTI
CHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
DLRAAAHALGEVTGESVTEAVLERIFSKFCIGK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292