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The map label for this gene is cobD [H]

Identifier: 37522017

GI number: 37522017

Start: 2604802

End: 2605746

Strand: Direct

Name: cobD [H]

Synonym: gvip340

Alternate gene names: 37522017

Gene position: 2604802-2605746 (Clockwise)

Preceding gene: 37522016

Following gene: 37522018

Centisome position: 55.91

GC content: 68.78

Gene sequence:

>945_bases
ATGCCCTCCGCCCTGGTCTTTGGCTGCGCCTTTTTGCTCGACGGGCTGCTGGGCGATCCGCCCCGCCTCTACCACCCGGT
GCAGGCGATGGGAGCGGTGATTGCCTCGGTCCAGCGTTGGGGACTGTCTCGCATCCAATCCCCGGGCGGTCGCCGCCTTC
TGGGGGTGGGGCTCACGGTGGGGCTCGCCGGGGGCAGTGCGGCGGGTGGCTGGGCGCTGATCGTCCTGGCGGCAGACATT
CAGCCCTGGTTGGGGGGGGTGGTCGAAGTGGTGCTGATGGCGGCGTGCTTTGCCACGCGCAGTCTGGCGGAGGCGGCCGG
AGCCGTGGCAATCGCCCTTGCCGCCGGGGATCTGCCCACGGCGCGACGGGTTTTGTCGCGCTACGTAGGGCGCGATACCG
AGGATCTGGGCGAGGCCGAAATCGCCCGGGCGGTGGTGGAGACGGTGGCTGAGAATACCACGGACGGGGTGACCGCTCCG
CTTTTTTACGCCGCCCTGGGCGGAGCGCCCCTGGCCCTAGCCTTCAAGGCGGTGAGCACCCTCGATTCGATGATCGGTTA
CCGCGATGAGCCCTACACCCACTTCGGCTGGTTCGCCGCGCGCAGCGACGACTGGCTCAACTGGATGCCGGCGAGGCTCA
CCGCCCTGACGGTCGCCATACTCTCGGGCCGCCCCGGCCGGGTCATCGCGATCGTGCGCCGCGACGCCCACCGCGATCCG
AGCCCCAACTCGGGGGTGAGCATCGCGGCCTACGCGGGTGCTCTGGATATACAACTGGGCGGCTTGAACCGTTACCGGGG
CATTATAAAGAAGAAACCGACCCTGGGCGATGCGCACTTGCCCCTGGACCCCCAGACGATTCGGCGCTCCGTCGGACTGC
TATGGCGAACGGCGGCGGTGTGGGCCGGGGTGAGCGTGGTGTTGTTGGTGGTAGCGCATGGGTAG

Upstream 100 bases:

>100_bases
CGCCATCTGGGGTCCCTGGTTGCGCTAGGGGCGGATGGAAAGTGGGTGCGGCCATCGCATCGGGCATACTGGACGGGGTT
TGTTTTCACACAGTCCGCCC

Downstream 100 bases:

>100_bases
AGCGTTGTTTGTCAAAATCGAGCGCGGTGTGGTCCCCAAGGAAATTTTCGACCGCTCTGTGTCCGCCCATATCGAATACG
TGCGCGCCCTGCGCTCAGAA

Product: cobalamin biosynthetic protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPSALVFGCAFLLDGLLGDPPRLYHPVQAMGAVIASVQRWGLSRIQSPGGRRLLGVGLTVGLAGGSAAGGWALIVLAADI
QPWLGGVVEVVLMAACFATRSLAEAAGAVAIALAAGDLPTARRVLSRYVGRDTEDLGEAEIARAVVETVAENTTDGVTAP
LFYAALGGAPLALAFKAVSTLDSMIGYRDEPYTHFGWFAARSDDWLNWMPARLTALTVAILSGRPGRVIAIVRRDAHRDP
SPNSGVSIAAYAGALDIQLGGLNRYRGIIKKKPTLGDAHLPLDPQTIRRSVGLLWRTAAVWAGVSVVLLVVAHG

Sequences:

>Translated_314_residues
MPSALVFGCAFLLDGLLGDPPRLYHPVQAMGAVIASVQRWGLSRIQSPGGRRLLGVGLTVGLAGGSAAGGWALIVLAADI
QPWLGGVVEVVLMAACFATRSLAEAAGAVAIALAAGDLPTARRVLSRYVGRDTEDLGEAEIARAVVETVAENTTDGVTAP
LFYAALGGAPLALAFKAVSTLDSMIGYRDEPYTHFGWFAARSDDWLNWMPARLTALTVAILSGRPGRVIAIVRRDAHRDP
SPNSGVSIAAYAGALDIQLGGLNRYRGIIKKKPTLGDAHLPLDPQTIRRSVGLLWRTAAVWAGVSVVLLVVAHG
>Mature_313_residues
PSALVFGCAFLLDGLLGDPPRLYHPVQAMGAVIASVQRWGLSRIQSPGGRRLLGVGLTVGLAGGSAAGGWALIVLAADIQ
PWLGGVVEVVLMAACFATRSLAEAAGAVAIALAAGDLPTARRVLSRYVGRDTEDLGEAEIARAVVETVAENTTDGVTAPL
FYAALGGAPLALAFKAVSTLDSMIGYRDEPYTHFGWFAARSDDWLNWMPARLTALTVAILSGRPGRVIAIVRRDAHRDPS
PNSGVSIAAYAGALDIQLGGLNRYRGIIKKKPTLGDAHLPLDPQTIRRSVGLLWRTAAVWAGVSVVLLVVAHG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 32842; Mature: 32711

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSALVFGCAFLLDGLLGDPPRLYHPVQAMGAVIASVQRWGLSRIQSPGGRRLLGVGLTV
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
GLAGGSAAGGWALIVLAADIQPWLGGVVEVVLMAACFATRSLAEAAGAVAIALAAGDLPT
EECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHH
ARRVLSRYVGRDTEDLGEAEIARAVVETVAENTTDGVTAPLFYAALGGAPLALAFKAVST
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LDSMIGYRDEPYTHFGWFAARSDDWLNWMPARLTALTVAILSGRPGRVIAIVRRDAHRDP
HHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
SPNSGVSIAAYAGALDIQLGGLNRYRGIIKKKPTLGDAHLPLDPQTIRRSVGLLWRTAAV
CCCCCEEEEEECCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WAGVSVVLLVVAHG
HHHHHHHHEEEECC
>Mature Secondary Structure 
PSALVFGCAFLLDGLLGDPPRLYHPVQAMGAVIASVQRWGLSRIQSPGGRRLLGVGLTV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
GLAGGSAAGGWALIVLAADIQPWLGGVVEVVLMAACFATRSLAEAAGAVAIALAAGDLPT
EECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHH
ARRVLSRYVGRDTEDLGEAEIARAVVETVAENTTDGVTAPLFYAALGGAPLALAFKAVST
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LDSMIGYRDEPYTHFGWFAARSDDWLNWMPARLTALTVAILSGRPGRVIAIVRRDAHRDP
HHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
SPNSGVSIAAYAGALDIQLGGLNRYRGIIKKKPTLGDAHLPLDPQTIRRSVGLLWRTAAV
CCCCCEEEEEECCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WAGVSVVLLVVAHG
HHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]