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The map label for this gene is pcrA [H]

Identifier: 37521963

GI number: 37521963

Start: 2554866

End: 2557247

Strand: Direct

Name: pcrA [H]

Synonym: glr2394

Alternate gene names: 37521963

Gene position: 2554866-2557247 (Clockwise)

Preceding gene: 37521961

Following gene: 37521964

Centisome position: 54.84

GC content: 63.73

Gene sequence:

>2382_bases
ATGGTCCTCGGTATGGATGCTTTGCTCAATCCTTCGCAGCAAAAAGCGGTGGAGCGCTTCAACGGTCCGCTGTTGGTGGT
GGCGGGTGCGGGTAGCGGCAAGACGCGGGTACTGACCTATCGCATCGCCCATCTCATCGAGACTTACCAGGTGGACCCCG
AGCACATCCTGGCGGTCACCTTCACCAACAAAGCAGCGGGCGAGATGAAGGAGCGCATCTTGCAACTCTTTTGCGAGCGC
ACCGCCAGAAGCCGCGGCGGTGAGGCGTTTGCCGCCCTGCCCGACCCCGAGCGGCGCGCCATCGCAGGCCGGGTGCGCCG
GGAATTGATTCAGCCTTTGTGGGTGGGGACTTTTCATGCCCTGTGCGCCCGTATGCTGCGCTACGACATCGACAAGTACA
CCGACCAACGCGGGCGCACCTGGCAGCGCAACTTCACGATCTTCGACGAATCGGACGTGCAGGATCAGATCAAAGACATC
GTCACCAAGCAGCTCAACCTGGACGACCGCCGCTACGTGCCCCGCTCGGTGCGCTTCGCCATCAGCGGCGCCAAAAACCA
GGGCCTTTCGCCGGAAGAATACCAGCACTCAGAAGGCGGTTCGCTGCGCGCCCGCACGATCGCCCAGGTCTACGAGCGCT
ACCAGGACCAGCTGGCGCGCAACAACGCCCTCGATTTCGACGATCTGATCTGGGTGCCGGTGCAGTTGTTGCGCCGGCGC
CCCGACGTGCTCGATTACTGGCACCGCCGCTTCCGTCACATCCTGGTCGACGAGTACCAGGACACCAACCGCACCCAGTA
CGACCTGATTCGCCTACTCGCCACCAACAACCAACCCAAAAGCGAGTGGGACTGGCGCGACCGGTCGGTCTTTGTGGTGG
GCGACGCGGACCAGGCCATCTACAGCTTTCGCCAGGCGGATTTTCGCATCCTGATGGATTTTCAGTCCGATTTTGGCGAC
GGCCTAGCCGACGACGAGACCGAGACGCTCATCAAGCTCGAAGAGAACTACCGCTCCTCGGCCACGATTCTCGATGTCGC
CAACGAACTGATCGCCAACAACATCGAGCGCATCGACAAGGTGCTGAGGGCCACCCGTCCGGCGGGCTTGCCCGTGTCGC
TGCACGAGGCGGAGGACGAGGTGGCCGAAGCTGAGTACATTGTCGGGCAACTGCGCAAACTCAAAGACGCCGACACTCGT
CCCTGGAACGACTTTGCGGTACTCTACCGCGTCAACGCCCAGTCGCAGCCCATCGAGCAGGCGCTTTCGCGCTGGGGGGT
GCCCTACACGGTGGTGGGCGGCTTGCGCTTCTACGACCGCCGTGAGATCAAGGACGTTCTGAGTTACCTCAAGGCGATCC
ACAATCCCGCCGACTCGGTCGCCCTCAAGCGCTCGATGTCCGCGCCGCGCCGCGGCATCGGCAAAACGACACTGGATAAG
CTCGAAGCCGGGGCCGGGATGCTCCAGACTTCGCTATGGCAACTGCTCACCGACGAGACGAGTATTAAAAATTTGGCCGG
GCGCACCAGCGGACCAATTCTCCAGTTCGTGCGCTTTGTCGAGCGCCTGCAGGAGCTGGCCGCCCGGGTGCGCGTCTCGG
AACTGCTCGATACGGTTCTCAAAGAGTCGGGCTACGTGCAGATGCTTCAGGAAGAAGGCACCGAGGAAGCCGAGGGGCGC
CTCGAAAACGTCCTGGAGTTGCGCTCGGTGGTCCAGCGCTTCGAAGAAGAAAGCGAAGATCCCGAAAACGCGACGCTCGA
AGCCTTTTTGGCCAATGTCTCGCTCGCGTCGGATCTGGACAATCTCGAAGCGGGGGCCGAGCGCATCTCGCTGATGACGC
TGCACGCCGCCAAGGGTCTGGAGTTTCCGGTGGTGTTTTTGGGCGGCCTCGAAGACGGCCTTTTTCCGCACTTTCGGGCG
ATTCAAGACGGTGACAGCGCCGCCATCGAGGAGGAGCGCCGATTGTGCTACGTGGGGATCACCCGCGCCAAGGAGCGTCT
GTTCTTGAGCTATGCCCAGGCCCGCCGCCTCTACGGCGACCGCCAGCCCGCCATCCCGTCGCAGTTTCTCAAGGAACTGC
CCGCCGAGAAACTGGCCGGTACCCGCGTGCGCAAGGGTCTTGCCGACCGGAGGCGCGACCGGGCCGAGCGGATTGCCGCC
CGCCCGGCCGCCGTCCCGCCGCTCGCCCCGCCCGCGCGGCGCAAAAGCCCCGCCCAGAACTGGGCGGTGGGCGACCGGGT
CGAGCACGACCAGTTCGGTCTCGGGCAGATTACCCACGTCCTGGGTGTGGGCGAGAAGCAGTACCTGGCCATCAGCTTCC
CCGGCCAGGGCAAAAAAGTGATCGACCCGCGCCTGGCGCCTCTGCGCAAGCTGGCTGAGTGA

Upstream 100 bases:

>100_bases
ATGTATAAGACACTACTGCATCCTTTGGGCTTTGTCATCGGCTCCACCACCGGCAGCGAGTAGGATAGTGCAGAAAAGAA
ACGACACAAGAGGCGGCCCG

Downstream 100 bases:

>100_bases
CACCTGCTCTTTAGAATCGAAAAGCCCAACAACCCATGCTCTCGCCATGAATTACCGCGAACACATCACCATTGACCCGC
GCATCCGCAGCGGAAAGCCC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MVLGMDALLNPSQQKAVERFNGPLLVVAGAGSGKTRVLTYRIAHLIETYQVDPEHILAVTFTNKAAGEMKERILQLFCER
TARSRGGEAFAALPDPERRAIAGRVRRELIQPLWVGTFHALCARMLRYDIDKYTDQRGRTWQRNFTIFDESDVQDQIKDI
VTKQLNLDDRRYVPRSVRFAISGAKNQGLSPEEYQHSEGGSLRARTIAQVYERYQDQLARNNALDFDDLIWVPVQLLRRR
PDVLDYWHRRFRHILVDEYQDTNRTQYDLIRLLATNNQPKSEWDWRDRSVFVVGDADQAIYSFRQADFRILMDFQSDFGD
GLADDETETLIKLEENYRSSATILDVANELIANNIERIDKVLRATRPAGLPVSLHEAEDEVAEAEYIVGQLRKLKDADTR
PWNDFAVLYRVNAQSQPIEQALSRWGVPYTVVGGLRFYDRREIKDVLSYLKAIHNPADSVALKRSMSAPRRGIGKTTLDK
LEAGAGMLQTSLWQLLTDETSIKNLAGRTSGPILQFVRFVERLQELAARVRVSELLDTVLKESGYVQMLQEEGTEEAEGR
LENVLELRSVVQRFEEESEDPENATLEAFLANVSLASDLDNLEAGAERISLMTLHAAKGLEFPVVFLGGLEDGLFPHFRA
IQDGDSAAIEEERRLCYVGITRAKERLFLSYAQARRLYGDRQPAIPSQFLKELPAEKLAGTRVRKGLADRRRDRAERIAA
RPAAVPPLAPPARRKSPAQNWAVGDRVEHDQFGLGQITHVLGVGEKQYLAISFPGQGKKVIDPRLAPLRKLAE

Sequences:

>Translated_793_residues
MVLGMDALLNPSQQKAVERFNGPLLVVAGAGSGKTRVLTYRIAHLIETYQVDPEHILAVTFTNKAAGEMKERILQLFCER
TARSRGGEAFAALPDPERRAIAGRVRRELIQPLWVGTFHALCARMLRYDIDKYTDQRGRTWQRNFTIFDESDVQDQIKDI
VTKQLNLDDRRYVPRSVRFAISGAKNQGLSPEEYQHSEGGSLRARTIAQVYERYQDQLARNNALDFDDLIWVPVQLLRRR
PDVLDYWHRRFRHILVDEYQDTNRTQYDLIRLLATNNQPKSEWDWRDRSVFVVGDADQAIYSFRQADFRILMDFQSDFGD
GLADDETETLIKLEENYRSSATILDVANELIANNIERIDKVLRATRPAGLPVSLHEAEDEVAEAEYIVGQLRKLKDADTR
PWNDFAVLYRVNAQSQPIEQALSRWGVPYTVVGGLRFYDRREIKDVLSYLKAIHNPADSVALKRSMSAPRRGIGKTTLDK
LEAGAGMLQTSLWQLLTDETSIKNLAGRTSGPILQFVRFVERLQELAARVRVSELLDTVLKESGYVQMLQEEGTEEAEGR
LENVLELRSVVQRFEEESEDPENATLEAFLANVSLASDLDNLEAGAERISLMTLHAAKGLEFPVVFLGGLEDGLFPHFRA
IQDGDSAAIEEERRLCYVGITRAKERLFLSYAQARRLYGDRQPAIPSQFLKELPAEKLAGTRVRKGLADRRRDRAERIAA
RPAAVPPLAPPARRKSPAQNWAVGDRVEHDQFGLGQITHVLGVGEKQYLAISFPGQGKKVIDPRLAPLRKLAE
>Mature_793_residues
MVLGMDALLNPSQQKAVERFNGPLLVVAGAGSGKTRVLTYRIAHLIETYQVDPEHILAVTFTNKAAGEMKERILQLFCER
TARSRGGEAFAALPDPERRAIAGRVRRELIQPLWVGTFHALCARMLRYDIDKYTDQRGRTWQRNFTIFDESDVQDQIKDI
VTKQLNLDDRRYVPRSVRFAISGAKNQGLSPEEYQHSEGGSLRARTIAQVYERYQDQLARNNALDFDDLIWVPVQLLRRR
PDVLDYWHRRFRHILVDEYQDTNRTQYDLIRLLATNNQPKSEWDWRDRSVFVVGDADQAIYSFRQADFRILMDFQSDFGD
GLADDETETLIKLEENYRSSATILDVANELIANNIERIDKVLRATRPAGLPVSLHEAEDEVAEAEYIVGQLRKLKDADTR
PWNDFAVLYRVNAQSQPIEQALSRWGVPYTVVGGLRFYDRREIKDVLSYLKAIHNPADSVALKRSMSAPRRGIGKTTLDK
LEAGAGMLQTSLWQLLTDETSIKNLAGRTSGPILQFVRFVERLQELAARVRVSELLDTVLKESGYVQMLQEEGTEEAEGR
LENVLELRSVVQRFEEESEDPENATLEAFLANVSLASDLDNLEAGAERISLMTLHAAKGLEFPVVFLGGLEDGLFPHFRA
IQDGDSAAIEEERRLCYVGITRAKERLFLSYAQARRLYGDRQPAIPSQFLKELPAEKLAGTRVRKGLADRRRDRAERIAA
RPAAVPPLAPPARRKSPAQNWAVGDRVEHDQFGLGQITHVLGVGEKQYLAISFPGQGKKVIDPRLAPLRKLAE

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=772, Percent_Identity=37.3056994818653, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI48994965, Length=709, Percent_Identity=37.2355430183357, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787196, Length=364, Percent_Identity=29.1208791208791, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=790, Percent_Identity=30.6329113924051, Blast_Score=246, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6324477, Length=476, Percent_Identity=22.6890756302521, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 89987; Mature: 89987

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLGMDALLNPSQQKAVERFNGPLLVVAGAGSGKTRVLTYRIAHLIETYQVDPEHILAVT
CEECCHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
FTNKAAGEMKERILQLFCERTARSRGGEAFAALPDPERRAIAGRVRRELIQPLWVGTFHA
ECCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LCARMLRYDIDKYTDQRGRTWQRNFTIFDESDVQDQIKDIVTKQLNLDDRRYVPRSVRFA
HHHHHHHHHHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHHCCCCCHHCCCHHHEEE
ISGAKNQGLSPEEYQHSEGGSLRARTIAQVYERYQDQLARNNALDFDDLIWVPVQLLRRR
EECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
PDVLDYWHRRFRHILVDEYQDTNRTQYDLIRLLATNNQPKSEWDWRDRSVFVVGDADQAI
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHH
YSFRQADFRILMDFQSDFGDGLADDETETLIKLEENYRSSATILDVANELIANNIERIDK
HHHHHCCHHEEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VLRATRPAGLPVSLHEAEDEVAEAEYIVGQLRKLKDADTRPWNDFAVLYRVNAQSQPIEQ
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHH
ALSRWGVPYTVVGGLRFYDRREIKDVLSYLKAIHNPADSVALKRSMSAPRRGIGKTTLDK
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHH
LEAGAGMLQTSLWQLLTDETSIKNLAGRTSGPILQFVRFVERLQELAARVRVSELLDTVL
HHHCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KESGYVQMLQEEGTEEAEGRLENVLELRSVVQRFEEESEDPENATLEAFLANVSLASDLD
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NLEAGAERISLMTLHAAKGLEFPVVFLGGLEDGLFPHFRAIQDGDSAAIEEERRLCYVGI
HHHHHHHHHHHHEEHHHCCCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHCCEEEEEH
TRAKERLFLSYAQARRLYGDRQPAIPSQFLKELPAEKLAGTRVRKGLADRRRDRAERIAA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
RPAAVPPLAPPARRKSPAQNWAVGDRVEHDQFGLGQITHVLGVGEKQYLAISFPGQGKKV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCC
IDPRLAPLRKLAE
CCCCHHHHHHHCC
>Mature Secondary Structure
MVLGMDALLNPSQQKAVERFNGPLLVVAGAGSGKTRVLTYRIAHLIETYQVDPEHILAVT
CEECCHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
FTNKAAGEMKERILQLFCERTARSRGGEAFAALPDPERRAIAGRVRRELIQPLWVGTFHA
ECCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LCARMLRYDIDKYTDQRGRTWQRNFTIFDESDVQDQIKDIVTKQLNLDDRRYVPRSVRFA
HHHHHHHHHHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHHCCCCCHHCCCHHHEEE
ISGAKNQGLSPEEYQHSEGGSLRARTIAQVYERYQDQLARNNALDFDDLIWVPVQLLRRR
EECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
PDVLDYWHRRFRHILVDEYQDTNRTQYDLIRLLATNNQPKSEWDWRDRSVFVVGDADQAI
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHH
YSFRQADFRILMDFQSDFGDGLADDETETLIKLEENYRSSATILDVANELIANNIERIDK
HHHHHCCHHEEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VLRATRPAGLPVSLHEAEDEVAEAEYIVGQLRKLKDADTRPWNDFAVLYRVNAQSQPIEQ
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHH
ALSRWGVPYTVVGGLRFYDRREIKDVLSYLKAIHNPADSVALKRSMSAPRRGIGKTTLDK
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHH
LEAGAGMLQTSLWQLLTDETSIKNLAGRTSGPILQFVRFVERLQELAARVRVSELLDTVL
HHHCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KESGYVQMLQEEGTEEAEGRLENVLELRSVVQRFEEESEDPENATLEAFLANVSLASDLD
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NLEAGAERISLMTLHAAKGLEFPVVFLGGLEDGLFPHFRAIQDGDSAAIEEERRLCYVGI
HHHHHHHHHHHHEEHHHCCCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHCCEEEEEH
TRAKERLFLSYAQARRLYGDRQPAIPSQFLKELPAEKLAGTRVRKGLADRRRDRAERIAA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
RPAAVPPLAPPARRKSPAQNWAVGDRVEHDQFGLGQITHVLGVGEKQYLAISFPGQGKKV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCC
IDPRLAPLRKLAE
CCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]