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The map label for this gene is alaS [H]

Identifier: 37521918

GI number: 37521918

Start: 2510472

End: 2511821

Strand: Reverse

Name: alaS [H]

Synonym: gll2349

Alternate gene names: 37521918

Gene position: 2511821-2510472 (Counterclockwise)

Preceding gene: 37521919

Following gene: 37521917

Centisome position: 53.91

GC content: 67.33

Gene sequence:

>1350_bases
ATGGAAGAATCGCGCGCCCAGGGACAGCCGGAAGCGGCGGCCACAGCCGACCGCAACGGCGAAGCAAAGACGGTTTTTGT
CGGGCACCGCACCCTTGCCGTTCCCGCGCAGGTGCTAGGCATCCTGGCGGGCGGCAAACCTTGCGCCGAGGCGGTAGCCG
GGGCCGAGGTGGTGGTGGTGCTCAGCCACAGTCCCTTTTTGGCCGAAGGCAGCGGCCAGGCCTGCGACCAGGGGGTGCTC
GCGGCCGAAAATCTGGTCGTGGCCGTCGAGGCGGTGCGCAGACAGGGTGCCGTCTACCTGCACAAAGGCAAAGTGCAGCG
CGGCGAACTGAAAGTCGGCGAGACCGTCCAGGCACAGGTGAACGCCATTCGCCGCCGGCTGGTGCAGACGCACCACAGCG
CCGTCCACCTGCTGCATGCCGCCCTGCGCAGGGTGCTGGGAGCCGAAATCGAGCAGCGCGGAGCGCTTGCAGCCTCTGAC
CGTTTGCGCTTCGAGTTTGCGGCCCCCCAGTCCCTGGGCGGGCAGGAGGTGGCCCGCGTCGAGCAGGTGGTCAATCTCTG
GGTGCTGGAAGGCCACCCTCTGGGGGTGGAGACGATGCCCGCCGAAAAAGCCCGGCAAATCGGGGCCTTGCCCTTTCCGG
GAGAGCGCTACGGCGAGCAGGTGCGTGTTATCGGCACCCCGGAGATCGGTTTCGAACTGTGTGGCGGCACCCACGTCTCC
AACACCATCGAAATTGGACCGTTCAAGATCGTCGGCCACAGCGAGCCGGAGGCGGGTGTACGGCGCCTCGAGGCGGTGGC
GGGGCTGGCCTTGCTCGATTACCTGCGTGCCCACGAGCAGGTCACCCGGATATTGGGCGAACAATTCAAAGCCCCCCTCG
CCCAGTTGCCCGAGCGGCTTGGGGCCGTACTGACCCAGGCGCAGTGGGCGATCTCCCAGGTCGAGGACCTCAAAGTCGCC
CTGGCGCTTGCCCGCTGCGAAGCCTGCCTGGGCGAAGTCGAGCAGTACGGCCAGTACCGGGTGCTGGTGGCCGATCTGGG
CGACACCGAACCCGCAGCACTGGCGGCGGCGGCCGAAGGGACGCTGAGCAAGCTGGGCGAAGGAACGGTGGTGCTGGGAT
CTTCTCCTGGGGCGGACGCGGTGGCATTCACCGTGGCATTTAGCCCAAAGGCGGTGGGCCTCGGTCTCAATGCCGGCCAG
TTTGCCGCCGCCCTCGCCCAACTGACCGGTGGCGGCGACACACAGGGTAACGCCCAAATCGCCCAGGCCGCAGGGCGGCA
GCCCACCAGACTCAAAGCGGCGCTGGATCTGGCCTTTGCGAAGCTCTACGACACCTTCAACGACACGTAG

Upstream 100 bases:

>100_bases
CGAGTGTGGGCGCCGCCGAGGGGTGCGGGGCCGCCTGCTGCCCTTAATACCCGGCGCGCTTGTCCCTTAGAAAAAGGGCA
GTCGCTTTCGGCATCACCAG

Downstream 100 bases:

>100_bases
GGGCGGGAAAATCTCGCTTCACCACCGCTTCCGAGGCATTGATGTTTTTTTAGAATGGGAAGGTAGCTTGCCAGTGCCTC
CGTCATGCCGACCGTCCTGA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MEESRAQGQPEAAATADRNGEAKTVFVGHRTLAVPAQVLGILAGGKPCAEAVAGAEVVVVLSHSPFLAEGSGQACDQGVL
AAENLVVAVEAVRRQGAVYLHKGKVQRGELKVGETVQAQVNAIRRRLVQTHHSAVHLLHAALRRVLGAEIEQRGALAASD
RLRFEFAAPQSLGGQEVARVEQVVNLWVLEGHPLGVETMPAEKARQIGALPFPGERYGEQVRVIGTPEIGFELCGGTHVS
NTIEIGPFKIVGHSEPEAGVRRLEAVAGLALLDYLRAHEQVTRILGEQFKAPLAQLPERLGAVLTQAQWAISQVEDLKVA
LALARCEACLGEVEQYGQYRVLVADLGDTEPAALAAAAEGTLSKLGEGTVVLGSSPGADAVAFTVAFSPKAVGLGLNAGQ
FAAALAQLTGGGDTQGNAQIAQAAGRQPTRLKAALDLAFAKLYDTFNDT

Sequences:

>Translated_449_residues
MEESRAQGQPEAAATADRNGEAKTVFVGHRTLAVPAQVLGILAGGKPCAEAVAGAEVVVVLSHSPFLAEGSGQACDQGVL
AAENLVVAVEAVRRQGAVYLHKGKVQRGELKVGETVQAQVNAIRRRLVQTHHSAVHLLHAALRRVLGAEIEQRGALAASD
RLRFEFAAPQSLGGQEVARVEQVVNLWVLEGHPLGVETMPAEKARQIGALPFPGERYGEQVRVIGTPEIGFELCGGTHVS
NTIEIGPFKIVGHSEPEAGVRRLEAVAGLALLDYLRAHEQVTRILGEQFKAPLAQLPERLGAVLTQAQWAISQVEDLKVA
LALARCEACLGEVEQYGQYRVLVADLGDTEPAALAAAAEGTLSKLGEGTVVLGSSPGADAVAFTVAFSPKAVGLGLNAGQ
FAAALAQLTGGGDTQGNAQIAQAAGRQPTRLKAALDLAFAKLYDTFNDT
>Mature_449_residues
MEESRAQGQPEAAATADRNGEAKTVFVGHRTLAVPAQVLGILAGGKPCAEAVAGAEVVVVLSHSPFLAEGSGQACDQGVL
AAENLVVAVEAVRRQGAVYLHKGKVQRGELKVGETVQAQVNAIRRRLVQTHHSAVHLLHAALRRVLGAEIEQRGALAASD
RLRFEFAAPQSLGGQEVARVEQVVNLWVLEGHPLGVETMPAEKARQIGALPFPGERYGEQVRVIGTPEIGFELCGGTHVS
NTIEIGPFKIVGHSEPEAGVRRLEAVAGLALLDYLRAHEQVTRILGEQFKAPLAQLPERLGAVLTQAQWAISQVEDLKVA
LALARCEACLGEVEQYGQYRVLVADLGDTEPAALAAAAEGTLSKLGEGTVVLGSSPGADAVAFTVAFSPKAVGLGLNAGQ
FAAALAQLTGGGDTQGNAQIAQAAGRQPTRLKAALDLAFAKLYDTFNDT

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=508, Percent_Identity=28.5433070866142, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI38569417, Length=252, Percent_Identity=29.3650793650794, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789048, Length=429, Percent_Identity=32.8671328671329, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17506981, Length=496, Percent_Identity=26.2096774193548, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324911, Length=427, Percent_Identity=25.7611241217799, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24582809, Length=250, Percent_Identity=31.6, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45552267, Length=250, Percent_Identity=31.6, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24658214, Length=240, Percent_Identity=28.75, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 46989; Mature: 46989

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEESRAQGQPEAAATADRNGEAKTVFVGHRTLAVPAQVLGILAGGKPCAEAVAGAEVVVV
CCCCCCCCCCCHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEE
LSHSPFLAEGSGQACDQGVLAAENLVVAVEAVRRQGAVYLHKGKVQRGELKVGETVQAQV
EECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHH
NAIRRRLVQTHHSAVHLLHAALRRVLGAEIEQRGALAASDRLRFEFAAPQSLGGQEVARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCHHHHHHH
EQVVNLWVLEGHPLGVETMPAEKARQIGALPFPGERYGEQVRVIGTPEIGFELCGGTHVS
HHHHHEEEECCCCCCCCCCCHHHHHHCCCCCCCCHHHCCEEEEEECCCCCCEECCCCCCC
NTIEIGPFKIVGHSEPEAGVRRLEAVAGLALLDYLRAHEQVTRILGEQFKAPLAQLPERL
CEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GAVLTQAQWAISQVEDLKVALALARCEACLGEVEQYGQYRVLVADLGDTEPAALAAAAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
TLSKLGEGTVVLGSSPGADAVAFTVAFSPKAVGLGLNAGQFAAALAQLTGGGDTQGNAQI
HHHHCCCCEEEECCCCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHH
AQAAGRQPTRLKAALDLAFAKLYDTFNDT
HHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEESRAQGQPEAAATADRNGEAKTVFVGHRTLAVPAQVLGILAGGKPCAEAVAGAEVVVV
CCCCCCCCCCCHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEE
LSHSPFLAEGSGQACDQGVLAAENLVVAVEAVRRQGAVYLHKGKVQRGELKVGETVQAQV
EECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHH
NAIRRRLVQTHHSAVHLLHAALRRVLGAEIEQRGALAASDRLRFEFAAPQSLGGQEVARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCHHHHHHH
EQVVNLWVLEGHPLGVETMPAEKARQIGALPFPGERYGEQVRVIGTPEIGFELCGGTHVS
HHHHHEEEECCCCCCCCCCCHHHHHHCCCCCCCCHHHCCEEEEEECCCCCCEECCCCCCC
NTIEIGPFKIVGHSEPEAGVRRLEAVAGLALLDYLRAHEQVTRILGEQFKAPLAQLPERL
CEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GAVLTQAQWAISQVEDLKVALALARCEACLGEVEQYGQYRVLVADLGDTEPAALAAAAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
TLSKLGEGTVVLGSSPGADAVAFTVAFSPKAVGLGLNAGQFAAALAQLTGGGDTQGNAQI
HHHHCCCCEEEECCCCCCCEEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHH
AQAAGRQPTRLKAALDLAFAKLYDTFNDT
HHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]