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The map label for this gene is pgk

Identifier: 37521882

GI number: 37521882

Start: 2476563

End: 2477786

Strand: Direct

Name: pgk

Synonym: gvip315

Alternate gene names: 37521882

Gene position: 2476563-2477786 (Clockwise)

Preceding gene: 37521881

Following gene: 37521883

Centisome position: 53.16

GC content: 62.83

Gene sequence:

>1224_bases
GTGGCGAAGAAAACGGTTGAGGCCCTGGGGGCGGCGGACCTCAAGGGTAAGCGGGCTCTGGTGCGGGTTGATTTTAATGT
TCCCCTCGACGAGAGCGGCCAGATCACAGACGATACGCGCATTCGCGCTGCGTTGCCCACCATTGGAATGTTGACCGGTG
GGGGGGCGCGGGTAATCCTGGTGAGCCACCTGGGGCGTCCCAAGGGTTTTGACGATCAGTTGCGCCTCACGCCGGTGGCC
AAGCGCCTGGGCGAACTGCTCGGCCAGACGGTCTACAAAGCCGACGACGTGATTGGCCCGGAAGTCGAAGAAGCGGTCAA
GAAGCTCGAAGACGGCGATGTGCTGCTGTTGGAGAACGTGCGCTTCTACCCGGAAGAAGAAAAAAACAACCCCGAATTTG
CCCAGAAGCTCGCGGGCCTCGCCGAACTTTATGTCAACGACGCCTTCGGCACCGCCCACCGCGCCCACGCCTCCACCGAA
GGCGTGGCGCGCTACTTGCGCCCGGCGGTGGCGGGGCTGCTTTTGCAAAAAGAACTCGAATACCTGGGCAAAGCCCTCGA
AAGCCCGGAACGGCCGGTGCTTGCCATCATGGGCGGCGCCAAAGTGAGCGACAAGATTCAGCTTATCCAGAACATGCTCA
CCAAGGTCGATTCGATCTTGATCGGCGGGGCGATGGCCTACACGTTCCTCAAAGCCCAAGGCGTGGATGTGGGAGCTTCC
CGCTGCGAGACGAGCACCACCAAAAAGGACGGCACGACCGTCGATTTGCTCCAGCTGGCCCTCGACCTGCTTGCCGAGGC
GAAGGAACGGGGCGTCACCTTCTTGCTGCCGGTCGACCACCGCACCAACGATAAGTTTGGTGACCTTGCCGACCCGCCGG
TCACCCCCGATGCCAACATCCCGGAGGGCCAGATGGCCCTGGACATCGGTCCCAAGACCGAGGAACTCTACGTGGCCGAA
GTCCAAAAATCGGGCACGGTGATCTGGAACGGTCCGATGGGCGTCTTCGAACTGCCGGGATTCAGCAAAGGCACCTTCGC
GGTGGCCCATGCCCTGGCTGAGAGCGACAACCTCTCGATCGTGGGCGGCGGCGACAGCGCCTCGGCGGCCGAGAAAGCCG
GTGTGGTCGACAAGCTCAGCCACGTGAGCACCGGTGGCGGTGCCTCGCTCGAATTTCTCGAAGGCAAGACCCTGCCCGGG
GTAGCGGCCCTCGACGAAGCCTGA

Upstream 100 bases:

>100_bases
CCCTCGACTGGCCCGGCGGCCAAAACTGATTCGCGCCGTCTTTGACTCCGGGGCGGGCTGTACCGGAAAATAATCGCGAA
GGACGTTGGGAGAACGGACG

Downstream 100 bases:

>100_bases
GGGGGCTTGCCAGATGTCCGGTGTCAGGGGATATCCTGACACCGAATAGCTTTTTGCAACCAAGGAGGCCCCTGTGAGCC
CATCTGCCGCCGCCCAGGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAKKTVEALGAADLKGKRALVRVDFNVPLDESGQITDDTRIRAALPTIGMLTGGGARVILVSHLGRPKGFDDQLRLTPVA
KRLGELLGQTVYKADDVIGPEVEEAVKKLEDGDVLLLENVRFYPEEEKNNPEFAQKLAGLAELYVNDAFGTAHRAHASTE
GVARYLRPAVAGLLLQKELEYLGKALESPERPVLAIMGGAKVSDKIQLIQNMLTKVDSILIGGAMAYTFLKAQGVDVGAS
RCETSTTKKDGTTVDLLQLALDLLAEAKERGVTFLLPVDHRTNDKFGDLADPPVTPDANIPEGQMALDIGPKTEELYVAE
VQKSGTVIWNGPMGVFELPGFSKGTFAVAHALAESDNLSIVGGGDSASAAEKAGVVDKLSHVSTGGGASLEFLEGKTLPG
VAALDEA

Sequences:

>Translated_407_residues
MAKKTVEALGAADLKGKRALVRVDFNVPLDESGQITDDTRIRAALPTIGMLTGGGARVILVSHLGRPKGFDDQLRLTPVA
KRLGELLGQTVYKADDVIGPEVEEAVKKLEDGDVLLLENVRFYPEEEKNNPEFAQKLAGLAELYVNDAFGTAHRAHASTE
GVARYLRPAVAGLLLQKELEYLGKALESPERPVLAIMGGAKVSDKIQLIQNMLTKVDSILIGGAMAYTFLKAQGVDVGAS
RCETSTTKKDGTTVDLLQLALDLLAEAKERGVTFLLPVDHRTNDKFGDLADPPVTPDANIPEGQMALDIGPKTEELYVAE
VQKSGTVIWNGPMGVFELPGFSKGTFAVAHALAESDNLSIVGGGDSASAAEKAGVVDKLSHVSTGGGASLEFLEGKTLPG
VAALDEA
>Mature_406_residues
AKKTVEALGAADLKGKRALVRVDFNVPLDESGQITDDTRIRAALPTIGMLTGGGARVILVSHLGRPKGFDDQLRLTPVAK
RLGELLGQTVYKADDVIGPEVEEAVKKLEDGDVLLLENVRFYPEEEKNNPEFAQKLAGLAELYVNDAFGTAHRAHASTEG
VARYLRPAVAGLLLQKELEYLGKALESPERPVLAIMGGAKVSDKIQLIQNMLTKVDSILIGGAMAYTFLKAQGVDVGASR
CETSTTKKDGTTVDLLQLALDLLAEAKERGVTFLLPVDHRTNDKFGDLADPPVTPDANIPEGQMALDIGPKTEELYVAEV
QKSGTVIWNGPMGVFELPGFSKGTFAVAHALAESDNLSIVGGGDSASAAEKAGVVDKLSHVSTGGGASLEFLEGKTLPGV
AALDEA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=47.4698795180723, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=45.7831325301205, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1789294, Length=399, Percent_Identity=41.8546365914787, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17508823, Length=422, Percent_Identity=47.1563981042654, Blast_Score=348, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=48.9156626506024, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=48.6810551558753, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24581203, Length=423, Percent_Identity=45.3900709219858, Blast_Score=318, Evalue=6e-87,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_GLOVI (Q7NI70)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925259.1
- ProteinModelPortal:   Q7NI70
- SMR:   Q7NI70
- GeneID:   2600715
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2313
- HOGENOM:   HBG453500
- OMA:   RITEFLP
- BioCyc:   GVIO251221:GLR2313-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43064; Mature: 42932

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 40-40 BINDING 121-121 BINDING 154-154 BINDING 205-205 BINDING 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKTVEALGAADLKGKRALVRVDFNVPLDESGQITDDTRIRAALPTIGMLTGGGARVIL
CCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHEECCCCCEEEECCCCEEEE
VSHLGRPKGFDDQLRLTPVAKRLGELLGQTVYKADDVIGPEVEEAVKKLEDGDVLLLENV
EEECCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECC
RFYPEEEKNNPEFAQKLAGLAELYVNDAFGTAHRAHASTEGVARYLRPAVAGLLLQKELE
EECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YLGKALESPERPVLAIMGGAKVSDKIQLIQNMLTKVDSILIGGAMAYTFLKAQGVDVGAS
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RCETSTTKKDGTTVDLLQLALDLLAEAKERGVTFLLPVDHRTNDKFGDLADPPVTPDANI
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PEGQMALDIGPKTEELYVAEVQKSGTVIWNGPMGVFELPGFSKGTFAVAHALAESDNLSI
CCCCEEEECCCCCCEEEEEEECCCCEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCEEE
VGGGDSASAAEKAGVVDKLSHVSTGGGASLEFLEGKTLPGVAALDEA
EECCCCCHHHHHHCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKTVEALGAADLKGKRALVRVDFNVPLDESGQITDDTRIRAALPTIGMLTGGGARVIL
CCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHEECCCCCEEEECCCCEEEE
VSHLGRPKGFDDQLRLTPVAKRLGELLGQTVYKADDVIGPEVEEAVKKLEDGDVLLLENV
EEECCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECC
RFYPEEEKNNPEFAQKLAGLAELYVNDAFGTAHRAHASTEGVARYLRPAVAGLLLQKELE
EECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YLGKALESPERPVLAIMGGAKVSDKIQLIQNMLTKVDSILIGGAMAYTFLKAQGVDVGAS
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RCETSTTKKDGTTVDLLQLALDLLAEAKERGVTFLLPVDHRTNDKFGDLADPPVTPDANI
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PEGQMALDIGPKTEELYVAEVQKSGTVIWNGPMGVFELPGFSKGTFAVAHALAESDNLSI
CCCCEEEECCCCCCEEEEEEECCCCEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCEEE
VGGGDSASAAEKAGVVDKLSHVSTGGGASLEFLEGKTLPGVAALDEA
EECCCCCHHHHHHCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292