Click here to switch to the map view.

The map label for this gene is sphR [H]

Identifier: 37521843

GI number: 37521843

Start: 2438065

End: 2438769

Strand: Direct

Name: sphR [H]

Synonym: glr2274

Alternate gene names: 37521843

Gene position: 2438065-2438769 (Clockwise)

Preceding gene: 37521842

Following gene: 37521844

Centisome position: 52.33

GC content: 63.26

Gene sequence:

>705_bases
GTGAAGCCGTATCAAATTCTCATCGTTGAAGACAACCACCAGCTGCGCGCGCTGCTTAACTGGCAACTGGAGCAGGACGG
CTATCGGCCCCACGCCGTGGGCACCCTCAAGGACGGACGCGACTGGCTGAGCGGTCACCGGCCGGATCTGGTGGTGCTCG
ATTTGCAGTTGCCCGACGGCGACGGCATCGACTTTTGCCGCTGGCTGCGCCAGCAGGTCGAATCGTACATTCTGATTTTG
TCCTGCCGGGGCACCGAAGGCGAGAAGGTCGTGGGCCTCAGGGCAGGGGCGGATGACTACCTGGCTAAACCCTTCGGCAT
GCAAGAATTTCGAGCGCGCATCGAGGCCCTGGCCCGACGGCTCAGGCAGCGCTCGATGCAGTTCGGGCCGCTGACCATCG
ACCCGGTGCAGCGCCGGGTGCTGCTCGACGGCCACCTCATCGATCTGACCCCGCAGGAATTCAGCCTGCTCTACGTGGTG
GCCAAAAGCCCGGGGGTGCCCTTCACCCGCGAGGAACTGCTCAAACTCGCCTGGCCCGAGCAGGTGGACAACCCCCGCAC
CGTCGATACCCACGTCCTCACGTTGCGCAAAAAAGTCGAATCGGACCCGCGCCACCCGCGCTTTTTGCAGACCGTGCGCG
GCATCGGTTATATGTTCCTCCTTGAGGAGCCTGGTTCCCAGGCGCGCTCACCCCTAAAATCTTGA

Upstream 100 bases:

>100_bases
CGCACAGAGACACAGATGGACAGCGGCCTGCGCCAGGAGTTGCTGGAGGCGATCGAAGGTCAGGCCATCCTGCTGCAGCA
GCGCTTAGAGGAGATCCTCG

Downstream 100 bases:

>100_bases
CAGGGAATCAAAAGGCGCAGCATGGCCCTACTGACCAAAGGCTTTGAAGTCGAGATGTATACCGGTACCGCTGCCGGGGA
TGTTGTCGGTTTGTCGCACC

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKPYQILIVEDNHQLRALLNWQLEQDGYRPHAVGTLKDGRDWLSGHRPDLVVLDLQLPDGDGIDFCRWLRQQVESYILIL
SCRGTEGEKVVGLRAGADDYLAKPFGMQEFRARIEALARRLRQRSMQFGPLTIDPVQRRVLLDGHLIDLTPQEFSLLYVV
AKSPGVPFTREELLKLAWPEQVDNPRTVDTHVLTLRKKVESDPRHPRFLQTVRGIGYMFLLEEPGSQARSPLKS

Sequences:

>Translated_234_residues
MKPYQILIVEDNHQLRALLNWQLEQDGYRPHAVGTLKDGRDWLSGHRPDLVVLDLQLPDGDGIDFCRWLRQQVESYILIL
SCRGTEGEKVVGLRAGADDYLAKPFGMQEFRARIEALARRLRQRSMQFGPLTIDPVQRRVLLDGHLIDLTPQEFSLLYVV
AKSPGVPFTREELLKLAWPEQVDNPRTVDTHVLTLRKKVESDPRHPRFLQTVRGIGYMFLLEEPGSQARSPLKS
>Mature_234_residues
MKPYQILIVEDNHQLRALLNWQLEQDGYRPHAVGTLKDGRDWLSGHRPDLVVLDLQLPDGDGIDFCRWLRQQVESYILIL
SCRGTEGEKVVGLRAGADDYLAKPFGMQEFRARIEALARRLRQRSMQFGPLTIDPVQRRVLLDGHLIDLTPQEFSLLYVV
AKSPGVPFTREELLKLAWPEQVDNPRTVDTHVLTLRKKVESDPRHPRFLQTVRGIGYMFLLEEPGSQARSPLKS

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=39.9103139013453, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789809, Length=237, Percent_Identity=35.4430379746835, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=36.7713004484305, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1788394, Length=219, Percent_Identity=36.0730593607306, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=37.3333333333333, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=33.1838565022422, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=35.3711790393013, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=36.4864864864865, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=34.2222222222222, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=33.4801762114537, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=30.1310043668122, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=32.2033898305085, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=32.4675324675325, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=28.695652173913, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26885; Mature: 26885

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYQILIVEDNHQLRALLNWQLEQDGYRPHAVGTLKDGRDWLSGHRPDLVVLDLQLPDG
CCCEEEEEEECCCCEEEEEEEEECCCCCCCCEEEECCCCHHHHCCCCCCEEEEEEECCCC
DGIDFCRWLRQQVESYILILSCRGTEGEKVVGLRAGADDYLAKPFGMQEFRARIEALARR
CCHHHHHHHHHHHHCEEEEEEECCCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHH
LRQRSMQFGPLTIDPVQRRVLLDGHLIDLTPQEFSLLYVVAKSPGVPFTREELLKLAWPE
HHHHCCCCCCEEECHHHHEEEECCEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHCCHH
QVDNPRTVDTHVLTLRKKVESDPRHPRFLQTVRGIGYMFLLEEPGSQARSPLKS
HCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCHHHCCCCCC
>Mature Secondary Structure
MKPYQILIVEDNHQLRALLNWQLEQDGYRPHAVGTLKDGRDWLSGHRPDLVVLDLQLPDG
CCCEEEEEEECCCCEEEEEEEEECCCCCCCCEEEECCCCHHHHCCCCCCEEEEEEECCCC
DGIDFCRWLRQQVESYILILSCRGTEGEKVVGLRAGADDYLAKPFGMQEFRARIEALARR
CCHHHHHHHHHHHHCEEEEEEECCCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHH
LRQRSMQFGPLTIDPVQRRVLLDGHLIDLTPQEFSLLYVVAKSPGVPFTREELLKLAWPE
HHHHCCCCCCEEECHHHHEEEECCEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHCCHH
QVDNPRTVDTHVLTLRKKVESDPRHPRFLQTVRGIGYMFLLEEPGSQARSPLKS
HCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]