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The map label for this gene is lacA [C]

Identifier: 37521747

GI number: 37521747

Start: 2327887

End: 2329128

Strand: Reverse

Name: lacA [C]

Synonym: gll2178

Alternate gene names: 37521747

Gene position: 2329128-2327887 (Counterclockwise)

Preceding gene: 37521748

Following gene: 37521746

Centisome position: 49.99

GC content: 59.82

Gene sequence:

>1242_bases
ATGAGTGAGACGAATACATTCACGTTGCTGAAGTTGGCGCGCTGGCGCGAGGCGGCCCTCAAAGACGCCTTGGGCTGGCT
GCCCAGGCTTCTGATGGGACCTGCCCTGCGCCGGACGCTCTACCGACAGCTGTTTCGGAAGATGGGAGACGGTGTATTTA
TTGACACCGATGTCGAAATTCTCAATGCCGCCGCGATCGAAATCGGTGACAAAGTCTGTGTACGCGCCGGAGTAAGACTC
GATGCCCGCAACCCCGGCAACCGCCTGGTGCTCAAAAGCAAAGCTTTTCTAGAGCGCGGGGTGATGATCATGGCGATGCG
ACAGACCACTATCGAGGTAGGCGAAGGGACCTTGGTGGGTCCCTACTCTGTATTGGCCGGCCCCGGGCATCTTACGATCG
GCAACAACTGCTTGATCGCTGCCCATGCCGGGATTTTTGCCAACAACCACCGCTTTGCGGATCCGGAGTTGACGATTCGC
GAACAGGGTGTCAGCCGAGAGGGAATCGTCATCGAGGACGACTGCTGGCTCGGCCACGCCGTGAGCGTGCTCGACGGCGT
CACCATCGGCCGGGGCAGCGTGATCGGTGCCGGAGCCGTCGTGACCCAGGACATTCCGCCCTACTCGGTGGCCGTTGGCG
TACCGGCTCGGGTGATCCGGAGGCGCGACGGCCTTTCGGTGACCCCTCAGAGTCGATTGGTGGAACAGGGAATGCCCGAT
ACGCTCAAACAGGCGTTGAGGCGGGCCGAGCAGGCTCTTGAACACCTTCAGAAGTTGCGGCATGAAGTCAGTGCTGTGCT
GTTGCAGGTGGTCTTTGCCGAGTTGCTGCACAGGCTGTTTGAGGAAATCCGCACTGCTCTGGATGTGGACACGGTCACCC
TGCTGCTGCCTAGATCGAGCAGCCGGGATCTCTTTGTGTACAACACCATCGGTCTTGAAGAAGAAATCGAGCAACAGGTG
CGCATTCCCCTTGGACAGGGAGTGGCCGGGCAGATTGCCGCAGACATCCAACCGCTCATCGTTGAGAACCTGGCCCAGGT
GGAGGTGGCCAGCCCCGTTTTGCGCAATAGAGGCGTTCAGTCCCTGCTTGGGGTTCCGCTGCATCTGGAGAAGCAGATGG
TGGGTGTTTTTCATGTGGGCAGTTTTCAGCGGCGGCACTTCACAGGCGAAGACACCTGCACGGTCGAGTCGGTCGCCGAG
CGGATCGGTCCACTGATCCGCATTGCGCAGTACTCTTCTTAG

Upstream 100 bases:

>100_bases
GCACTGCGGGGCGACCAATGGGACCGGTCCGGGCTGGCGCCCTTCATCCGCTCAGAAAGACCGCGTCCTGACCCCTACCC
ACAGCAAGCGGAGAGTCGGA

Downstream 100 bases:

>100_bases
GAGTACTGTAGCCAAGCCGTACCGACTTTGTCGCCCAAGGCCGCCAGAAACCCTAATCGTAGACAGCGGATACGGGTTGG
CTAAATTTAAGGACTCGTCA

Product: hypothetical protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSETNTFTLLKLARWREAALKDALGWLPRLLMGPALRRTLYRQLFRKMGDGVFIDTDVEILNAAAIEIGDKVCVRAGVRL
DARNPGNRLVLKSKAFLERGVMIMAMRQTTIEVGEGTLVGPYSVLAGPGHLTIGNNCLIAAHAGIFANNHRFADPELTIR
EQGVSREGIVIEDDCWLGHAVSVLDGVTIGRGSVIGAGAVVTQDIPPYSVAVGVPARVIRRRDGLSVTPQSRLVEQGMPD
TLKQALRRAEQALEHLQKLRHEVSAVLLQVVFAELLHRLFEEIRTALDVDTVTLLLPRSSSRDLFVYNTIGLEEEIEQQV
RIPLGQGVAGQIAADIQPLIVENLAQVEVASPVLRNRGVQSLLGVPLHLEKQMVGVFHVGSFQRRHFTGEDTCTVESVAE
RIGPLIRIAQYSS

Sequences:

>Translated_413_residues
MSETNTFTLLKLARWREAALKDALGWLPRLLMGPALRRTLYRQLFRKMGDGVFIDTDVEILNAAAIEIGDKVCVRAGVRL
DARNPGNRLVLKSKAFLERGVMIMAMRQTTIEVGEGTLVGPYSVLAGPGHLTIGNNCLIAAHAGIFANNHRFADPELTIR
EQGVSREGIVIEDDCWLGHAVSVLDGVTIGRGSVIGAGAVVTQDIPPYSVAVGVPARVIRRRDGLSVTPQSRLVEQGMPD
TLKQALRRAEQALEHLQKLRHEVSAVLLQVVFAELLHRLFEEIRTALDVDTVTLLLPRSSSRDLFVYNTIGLEEEIEQQV
RIPLGQGVAGQIAADIQPLIVENLAQVEVASPVLRNRGVQSLLGVPLHLEKQMVGVFHVGSFQRRHFTGEDTCTVESVAE
RIGPLIRIAQYSS
>Mature_412_residues
SETNTFTLLKLARWREAALKDALGWLPRLLMGPALRRTLYRQLFRKMGDGVFIDTDVEILNAAAIEIGDKVCVRAGVRLD
ARNPGNRLVLKSKAFLERGVMIMAMRQTTIEVGEGTLVGPYSVLAGPGHLTIGNNCLIAAHAGIFANNHRFADPELTIRE
QGVSREGIVIEDDCWLGHAVSVLDGVTIGRGSVIGAGAVVTQDIPPYSVAVGVPARVIRRRDGLSVTPQSRLVEQGMPDT
LKQALRRAEQALEHLQKLRHEVSAVLLQVVFAELLHRLFEEIRTALDVDTVTLLLPRSSSRDLFVYNTIGLEEEIEQQVR
IPLGQGVAGQIAADIQPLIVENLAQVEVASPVLRNRGVQSLLGVPLHLEKQMVGVFHVGSFQRRHFTGEDTCTVESVAER
IGPLIRIAQYSS

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=105, Percent_Identity=42.8571428571429, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788345, Length=222, Percent_Identity=27.9279279279279, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 45277; Mature: 45146

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETNTFTLLKLARWREAALKDALGWLPRLLMGPALRRTLYRQLFRKMGDGVFIDTDVEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECCHHH
LNAAAIEIGDKVCVRAGVRLDARNPGNRLVLKSKAFLERGVMIMAMRQTTIEVGEGTLVG
HHHHHHHHCCHHHHHCCCEEECCCCCCEEEEECHHHHHCCEEEEEEHHHEEEECCCCEEC
PYSVLAGPGHLTIGNNCLIAAHAGIFANNHRFADPELTIREQGVSREGIVIEDDCWLGHA
CHHHHCCCCEEEECCCEEEEEECCEEECCCCCCCCCEEEHHCCCCCCCEEEECCCCHHHH
VSVLDGVTIGRGSVIGAGAVVTQDIPPYSVAVGVPARVIRRRDGLSVTPQSRLVEQGMPD
HHHHCCCEECCCCEEECCCEEECCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHCCCCH
TLKQALRRAEQALEHLQKLRHEVSAVLLQVVFAELLHRLFEEIRTALDVDTVTLLLPRSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
SRDLFVYNTIGLEEEIEQQVRIPLGQGVAGQIAADIQPLIVENLAQVEVASPVLRNRGVQ
CCCEEEEEECCCHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
SLLGVPLHLEKQMVGVFHVGSFQRRHFTGEDTCTVESVAERIGPLIRIAQYSS
HHHCCCCEEHHHHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SETNTFTLLKLARWREAALKDALGWLPRLLMGPALRRTLYRQLFRKMGDGVFIDTDVEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECCHHH
LNAAAIEIGDKVCVRAGVRLDARNPGNRLVLKSKAFLERGVMIMAMRQTTIEVGEGTLVG
HHHHHHHHCCHHHHHCCCEEECCCCCCEEEEECHHHHHCCEEEEEEHHHEEEECCCCEEC
PYSVLAGPGHLTIGNNCLIAAHAGIFANNHRFADPELTIREQGVSREGIVIEDDCWLGHA
CHHHHCCCCEEEECCCEEEEEECCEEECCCCCCCCCEEEHHCCCCCCCEEEECCCCHHHH
VSVLDGVTIGRGSVIGAGAVVTQDIPPYSVAVGVPARVIRRRDGLSVTPQSRLVEQGMPD
HHHHCCCEECCCCEEECCCEEECCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHCCCCH
TLKQALRRAEQALEHLQKLRHEVSAVLLQVVFAELLHRLFEEIRTALDVDTVTLLLPRSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
SRDLFVYNTIGLEEEIEQQVRIPLGQGVAGQIAADIQPLIVENLAQVEVASPVLRNRGVQ
CCCEEEEEECCCHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
SLLGVPLHLEKQMVGVFHVGSFQRRHFTGEDTCTVESVAERIGPLIRIAQYSS
HHHCCCCEEHHHHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]