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The map label for this gene is trmJ [H]

Identifier: 37521709

GI number: 37521709

Start: 2291337

End: 2292077

Strand: Direct

Name: trmJ [H]

Synonym: glr2140

Alternate gene names: 37521709

Gene position: 2291337-2292077 (Clockwise)

Preceding gene: 37521708

Following gene: 37521710

Centisome position: 49.18

GC content: 62.48

Gene sequence:

>741_bases
TTGAACGTCCGCATCGTCCTGGTTGCAACGCAGGGGGCACGGAACCTGGGGGCGATCGCCCGGGTGATCAAAAATTTTGA
TCTGTCCGAGTTGTGGCTGGTGGCGCCCGAGTGCTCGCCCAAGGACGAGGAGGCGCGCCACATGGCCGTGCACGCAGCGG
ATGTGCTCGATCAAGCCCGGATCGTAGCGAGCCTTTCGCAGGCCCTGGCAGATTGCACTCGGGTGGCCGGCACCACAGCC
CGCACCCGCACGGTCAGCGATCCGCCCCTTTTACCCGCCGAAGGAGCGCGCTGGCTGCGGGCGGACAAAGGAACGGCAGC
CTACGTGTTTGGCCCCGAAGATCGCGGGCTCAGCAATGAAGAGCTGGCCCACTGCCAGCGCACCGTGCAGATTCCCACTG
GTGCAGCTTACCCGTCGCTCAATCTGGCGCAAGCTGTGGGAATCTGCGCCTACGCGCTTTTTACAGATAGCAAGCCTCCC
TCACCCGTCTTTCAGAACGAGCCGATGCCTGAGGCGGAAGTGCTGGAGGGCTTCTATGGCCATCTAGAAAGGACGCTACT
GGCTATTGGCTATCTGCAGGAGCACACGGCGGAGCGCAAACTCGAAAAGTTTCGCCGCCTATTTAACCGGGCGGGCCTCA
CTGCCCAGGAAGTAGCCCTGCTGCGCGGTGTGCTCAGACAGATCGACTGGGCAAGTACCCAAAACCCAGCTCGCCAACTC
CCCCTGACACCTGACACCTGA

Upstream 100 bases:

>100_bases
GAGATTGAGTCGGTCCCCGGTATCCGCAAGGCGTATACGGTCAATCTTTGAACACCACTTTTCGGTGTAGGATGCGCGGA
CAAGCGTAGGATCTGACTAC

Downstream 100 bases:

>100_bases
CACCTGTACCATAGTCCGGATGCTTATCCGTTCGAACCGCCGCGACCTATTGCGCTCCGGCCTGCTGCTCGCTCCGCTGT
TGGCAGGCTGCAGTCGGACG

Product: methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNVRIVLVATQGARNLGAIARVIKNFDLSELWLVAPECSPKDEEARHMAVHAADVLDQARIVASLSQALADCTRVAGTTA
RTRTVSDPPLLPAEGARWLRADKGTAAYVFGPEDRGLSNEELAHCQRTVQIPTGAAYPSLNLAQAVGICAYALFTDSKPP
SPVFQNEPMPEAEVLEGFYGHLERTLLAIGYLQEHTAERKLEKFRRLFNRAGLTAQEVALLRGVLRQIDWASTQNPARQL
PLTPDT

Sequences:

>Translated_246_residues
MNVRIVLVATQGARNLGAIARVIKNFDLSELWLVAPECSPKDEEARHMAVHAADVLDQARIVASLSQALADCTRVAGTTA
RTRTVSDPPLLPAEGARWLRADKGTAAYVFGPEDRGLSNEELAHCQRTVQIPTGAAYPSLNLAQAVGICAYALFTDSKPP
SPVFQNEPMPEAEVLEGFYGHLERTLLAIGYLQEHTAERKLEKFRRLFNRAGLTAQEVALLRGVLRQIDWASTQNPARQL
PLTPDT
>Mature_246_residues
MNVRIVLVATQGARNLGAIARVIKNFDLSELWLVAPECSPKDEEARHMAVHAADVLDQARIVASLSQALADCTRVAGTTA
RTRTVSDPPLLPAEGARWLRADKGTAAYVFGPEDRGLSNEELAHCQRTVQIPTGAAYPSLNLAQAVGICAYALFTDSKPP
SPVFQNEPMPEAEVLEGFYGHLERTLLAIGYLQEHTAERKLEKFRRLFNRAGLTAQEVALLRGVLRQIDWASTQNPARQL
PLTPDT

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=243, Percent_Identity=42.798353909465, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1790865, Length=169, Percent_Identity=36.6863905325444, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26920; Mature: 26920

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRIVLVATQGARNLGAIARVIKNFDLSELWLVAPECSPKDEEARHMAVHAADVLDQAR
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
IVASLSQALADCTRVAGTTARTRTVSDPPLLPAEGARWLRADKGTAAYVFGPEDRGLSNE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEECCCCCEEEEECCCCCCCCHH
ELAHCQRTVQIPTGAAYPSLNLAQAVGICAYALFTDSKPPSPVFQNEPMPEAEVLEGFYG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
HLERTLLAIGYLQEHTAERKLEKFRRLFNRAGLTAQEVALLRGVLRQIDWASTQNPARQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHCC
PLTPDT
CCCCCC
>Mature Secondary Structure
MNVRIVLVATQGARNLGAIARVIKNFDLSELWLVAPECSPKDEEARHMAVHAADVLDQAR
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
IVASLSQALADCTRVAGTTARTRTVSDPPLLPAEGARWLRADKGTAAYVFGPEDRGLSNE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEECCCCCEEEEECCCCCCCCHH
ELAHCQRTVQIPTGAAYPSLNLAQAVGICAYALFTDSKPPSPVFQNEPMPEAEVLEGFYG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
HLERTLLAIGYLQEHTAERKLEKFRRLFNRAGLTAQEVALLRGVLRQIDWASTQNPARQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHCC
PLTPDT
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA