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The map label for this gene is btuB [H]

Identifier: 37521620

GI number: 37521620

Start: 2201644

End: 2203770

Strand: Direct

Name: btuB [H]

Synonym: glr2051

Alternate gene names: 37521620

Gene position: 2201644-2203770 (Clockwise)

Preceding gene: 37521618

Following gene: 37521621

Centisome position: 47.26

GC content: 64.32

Gene sequence:

>2127_bases
ATGCGATTCTCCTGCCTGTCCGTCCTTTTGATCCTGGGTGCGGCTCTCGTTGCGCCGGCCTATGCCCAGCAGACCCCCGA
CCTTCCCGGAGCGCCCGACACCACCCAGGCGGCCGAATCGGACCTCGACGAGGTGACCGTCACCGCGAACCGCCGGGCGA
CGCCGCGCTCGAAGACCCCGGCCACGGTGTCGATCAAGACCCGCTCCGAACTCGATCGCGAGCAGCCGCTGTTGCGCACG
GTGGCCGATGCGCTGCAGACCTTGCCGGGGATCACCATCAACCGCCTGGGCTTGCTGAGCGGGGCGGCGAATATCCGCGG
CCTGACCGGCGAGCGCATCGCGGTGCTGGTAGACGGTGAGCGGCTGCCGAATCTGGAATTCGGCCCGGATCTCGGTTCTG
TGGACCCATTCCGGGTCGAGCGGCTGGAGGTGCTCAAAGGCCCGGCCTCGTCGATCTACGGGGCGGACGCCTTTGGGGGC
GTCATCAACATCATCACGACGCTGCCGCGCTTCGACACGCCGCCCAAGGTGCGCACGTATCTCTACGGCGGCAACTTCTC
AGAAATCGGCGGCAACGTCGAATTTCAGGGGCCGAACGTAGTGGCGGGGTTGTCTTTGCGCACGGCGGGAGACGCCAAAG
ACGGCCTCTCGCGGCCCATTCCCACCAACGGCACCGGCTACGACGCCTTCGACGCCTACGCGAGCGGCCGGATCGACTTC
GATGCGACCAATCACCTGGAGCTGCGCTTCGACCGCTACCGCCAGAGTTACGCCGATCTCAACGGTTTTCCGACTCCGCC
TTTTGTCTTTGCCCAGAACCAATTTCGCAACCGCGACCGCTACTCGATCGCCTACATCAACGACGGGGCGCCGGGAGGAA
CGAGCTTTGCCCTCAGGGCCAACTACCAAAAAAACGAGCGGCGATTCGACAACACCACGGCGGTGACAATCCCGGTGTTT
GCCAGCCCATTTGCTGTCGGGGGGAACGGAACGTCCAGCCTGTTCGGGACGCCCAGCCGGCAGGCCGCATTCTTCCCGCC
CGCGCCGCCGACGTTTATCACCCTCACCACCCCCAGTTCGACCTACAGCCTCACCGAGACCTACGGCCTCTCCGCGCAGG
CCAACACGGCCCTCGGTTCGGCGGTGCTCACCTACGGCTACGACTTCAGCCAGGACGCTTCGACCTCGATCGATCGCTTT
GCTGTGCCTTCGGTGCAATCGCCGCTGGCCACCCGCAGCTTCAACGGCGTCTTTCTGCAGGGTTCCTACGACATTACCCC
GAGCTTCACCGTGGCGGGCGGGGTACGCTACGACACCTACGACCAGAACACGAACACGGGTCTGGTCAACAACGCCAACC
GCGTCACCTTCAACGCCGGGGCCATTTACTCCATCACCCCCGAACTGGCCCTGCGGGCAAATTTTGCCCAGGGCTTCCGG
CCCCCGAGCCTGCTGTTTTTGTTCGGCAGCAGCCCGGAGGGCACCTTCTTCGCCCCGAGCGCCGGCAACGTCCAGGCAAA
TCCCAACTTGCGGCCGGAGCGGGCCGACAACTTCGACATCGGCATCAACTACACGAGTCCCGAGTTTCGCGCCGGGATCA
CTTACTTCAACAACCAGGTCACCGACTTTCTCGGCTTCGGGCCGCTCGTTGCCTTTGGAGCGCCCCCCTTCGGACCGCCG
CCCCCTTCCCAAACCGTGCTCAACAAAAATGTCCAGCTGCAGGGGCTCGAATTTTCGAGCGCTTACTTTTTTTCGCCCCA
GGCTTTTTTTGAAGCCAACCTCACCTACGTCGACGGCCGCGACGGCTCCGGGCTGCCGCTGTCGCAACTGGAAGTTTTTC
CGCTCACGGCCCTGTTGCGCCTGGTCTACGACGACAAGACCTTCAACGCCCTGCTGCAGTCGCGCATCTACGGCGGCCAG
GGTTCGGTGATCGGCAACAACGGTGTGGTCGGCCCCGGCACCCCCCCGGCGGGTGTGCTCGATCTGAGCTTCGGTTACCG
CTTCGTCCCCAATGTGCAGCTGACGGTGAGCGTCGAGAACGTCACCAACGCCCAGTACATCTATCCGACCAGCGGCTTTG
TGGCGCCGGGGGCGAGGCTATTGGGGGCGGTGAGGGCGGAGTTTTGA

Upstream 100 bases:

>100_bases
TCACAGGTGCGGGATTTTCCGGTTTGCCTATCCCCCCTGCCCGGATTGGGTAATCGCTTCCGGTATGGCATCAATCGGAG
GGTGAGCGAGTGAACAAACG

Downstream 100 bases:

>100_bases
TCGCTGTTATGCTGGCTTCAGGGTGATTGAGCCGCCCAGCCGACTTGCCTGGGATGCGTTTTTTCTCGAATTGGGTCAGG
AGAACCGCGATGGAAAAGTC

Product: hypothetical protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MRFSCLSVLLILGAALVAPAYAQQTPDLPGAPDTTQAAESDLDEVTVTANRRATPRSKTPATVSIKTRSELDREQPLLRT
VADALQTLPGITINRLGLLSGAANIRGLTGERIAVLVDGERLPNLEFGPDLGSVDPFRVERLEVLKGPASSIYGADAFGG
VINIITTLPRFDTPPKVRTYLYGGNFSEIGGNVEFQGPNVVAGLSLRTAGDAKDGLSRPIPTNGTGYDAFDAYASGRIDF
DATNHLELRFDRYRQSYADLNGFPTPPFVFAQNQFRNRDRYSIAYINDGAPGGTSFALRANYQKNERRFDNTTAVTIPVF
ASPFAVGGNGTSSLFGTPSRQAAFFPPAPPTFITLTTPSSTYSLTETYGLSAQANTALGSAVLTYGYDFSQDASTSIDRF
AVPSVQSPLATRSFNGVFLQGSYDITPSFTVAGGVRYDTYDQNTNTGLVNNANRVTFNAGAIYSITPELALRANFAQGFR
PPSLLFLFGSSPEGTFFAPSAGNVQANPNLRPERADNFDIGINYTSPEFRAGITYFNNQVTDFLGFGPLVAFGAPPFGPP
PPSQTVLNKNVQLQGLEFSSAYFFSPQAFFEANLTYVDGRDGSGLPLSQLEVFPLTALLRLVYDDKTFNALLQSRIYGGQ
GSVIGNNGVVGPGTPPAGVLDLSFGYRFVPNVQLTVSVENVTNAQYIYPTSGFVAPGARLLGAVRAEF

Sequences:

>Translated_708_residues
MRFSCLSVLLILGAALVAPAYAQQTPDLPGAPDTTQAAESDLDEVTVTANRRATPRSKTPATVSIKTRSELDREQPLLRT
VADALQTLPGITINRLGLLSGAANIRGLTGERIAVLVDGERLPNLEFGPDLGSVDPFRVERLEVLKGPASSIYGADAFGG
VINIITTLPRFDTPPKVRTYLYGGNFSEIGGNVEFQGPNVVAGLSLRTAGDAKDGLSRPIPTNGTGYDAFDAYASGRIDF
DATNHLELRFDRYRQSYADLNGFPTPPFVFAQNQFRNRDRYSIAYINDGAPGGTSFALRANYQKNERRFDNTTAVTIPVF
ASPFAVGGNGTSSLFGTPSRQAAFFPPAPPTFITLTTPSSTYSLTETYGLSAQANTALGSAVLTYGYDFSQDASTSIDRF
AVPSVQSPLATRSFNGVFLQGSYDITPSFTVAGGVRYDTYDQNTNTGLVNNANRVTFNAGAIYSITPELALRANFAQGFR
PPSLLFLFGSSPEGTFFAPSAGNVQANPNLRPERADNFDIGINYTSPEFRAGITYFNNQVTDFLGFGPLVAFGAPPFGPP
PPSQTVLNKNVQLQGLEFSSAYFFSPQAFFEANLTYVDGRDGSGLPLSQLEVFPLTALLRLVYDDKTFNALLQSRIYGGQ
GSVIGNNGVVGPGTPPAGVLDLSFGYRFVPNVQLTVSVENVTNAQYIYPTSGFVAPGARLLGAVRAEF
>Mature_708_residues
MRFSCLSVLLILGAALVAPAYAQQTPDLPGAPDTTQAAESDLDEVTVTANRRATPRSKTPATVSIKTRSELDREQPLLRT
VADALQTLPGITINRLGLLSGAANIRGLTGERIAVLVDGERLPNLEFGPDLGSVDPFRVERLEVLKGPASSIYGADAFGG
VINIITTLPRFDTPPKVRTYLYGGNFSEIGGNVEFQGPNVVAGLSLRTAGDAKDGLSRPIPTNGTGYDAFDAYASGRIDF
DATNHLELRFDRYRQSYADLNGFPTPPFVFAQNQFRNRDRYSIAYINDGAPGGTSFALRANYQKNERRFDNTTAVTIPVF
ASPFAVGGNGTSSLFGTPSRQAAFFPPAPPTFITLTTPSSTYSLTETYGLSAQANTALGSAVLTYGYDFSQDASTSIDRF
AVPSVQSPLATRSFNGVFLQGSYDITPSFTVAGGVRYDTYDQNTNTGLVNNANRVTFNAGAIYSITPELALRANFAQGFR
PPSLLFLFGSSPEGTFFAPSAGNVQANPNLRPERADNFDIGINYTSPEFRAGITYFNNQVTDFLGFGPLVAFGAPPFGPP
PPSQTVLNKNVQLQGLEFSSAYFFSPQAFFEANLTYVDGRDGSGLPLSQLEVFPLTALLRLVYDDKTFNALLQSRIYGGQ
GSVIGNNGVVGPGTPPAGVLDLSFGYRFVPNVQLTVSVENVTNAQYIYPTSGFVAPGARLLGAVRAEF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=136, Percent_Identity=40.4411764705882, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788478, Length=135, Percent_Identity=35.5555555555556, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786798, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 75839; Mature: 75839

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
0.1 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.1 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSCLSVLLILGAALVAPAYAQQTPDLPGAPDTTQAAESDLDEVTVTANRRATPRSKTP
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCC
ATVSIKTRSELDREQPLLRTVADALQTLPGITINRLGLLSGAANIRGLTGERIAVLVDGE
EEEEEECHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCEEEEEECCC
RLPNLEFGPDLGSVDPFRVERLEVLKGPASSIYGADAFGGVINIITTLPRFDTPPKVRTY
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
LYGGNFSEIGGNVEFQGPNVVAGLSLRTAGDAKDGLSRPIPTNGTGYDAFDAYASGRIDF
EECCCHHHHCCEEEECCCCEEEEEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHCCEEEC
DATNHLELRFDRYRQSYADLNGFPTPPFVFAQNQFRNRDRYSIAYINDGAPGGTSFALRA
CCCCCEEEEHHHHHHHHHCCCCCCCCCEEEECHHHCCCCCEEEEEEECCCCCCCEEEEEE
NYQKNERRFDNTTAVTIPVFASPFAVGGNGTSSLFGTPSRQAAFFPPAPPTFITLTTPSS
CCCCCHHHCCCCEEEEEEEEECCEEECCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCC
TYSLTETYGLSAQANTALGSAVLTYGYDFSQDASTSIDRFAVPSVQSPLATRSFNGVFLQ
CEEEEHCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCHHEECCCCCCCCHHCCCCCEEEE
GSYDITPSFTVAGGVRYDTYDQNTNTGLVNNANRVTFNAGAIYSITPELALRANFAQGFR
CCCCCCCCEEEECCEEECCCCCCCCCCEEECCCEEEEECCEEEEECHHHHEEECHHHCCC
PPSLLFLFGSSPEGTFFAPSAGNVQANPNLRPERADNFDIGINYTSPEFRAGITYFNNQV
CCEEEEEECCCCCCCEECCCCCCEECCCCCCCCCCCCEEEEEEECCCHHHCCEEEECCHH
TDFLGFGPLVAFGAPPFGPPPPSQTVLNKNVQLQGLEFSSAYFFSPQAFFEANLTYVDGR
HHHHCCCHHHHCCCCCCCCCCCCHHHHCCCEEEEEEEECCEEEECCHHHEEECEEEEECC
DGSGLPLSQLEVFPLTALLRLVYDDKTFNALLQSRIYGGQGSVIGNNGVVGPGTPPAGVL
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEECCCCEECCCCCCCCEE
DLSFGYRFVPNVQLTVSVENVTNAQYIYPTSGFVAPGARLLGAVRAEF
ECCCCEEECCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRFSCLSVLLILGAALVAPAYAQQTPDLPGAPDTTQAAESDLDEVTVTANRRATPRSKTP
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCC
ATVSIKTRSELDREQPLLRTVADALQTLPGITINRLGLLSGAANIRGLTGERIAVLVDGE
EEEEEECHHHCCHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCEEEEEECCC
RLPNLEFGPDLGSVDPFRVERLEVLKGPASSIYGADAFGGVINIITTLPRFDTPPKVRTY
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
LYGGNFSEIGGNVEFQGPNVVAGLSLRTAGDAKDGLSRPIPTNGTGYDAFDAYASGRIDF
EECCCHHHHCCEEEECCCCEEEEEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHCCEEEC
DATNHLELRFDRYRQSYADLNGFPTPPFVFAQNQFRNRDRYSIAYINDGAPGGTSFALRA
CCCCCEEEEHHHHHHHHHCCCCCCCCCEEEECHHHCCCCCEEEEEEECCCCCCCEEEEEE
NYQKNERRFDNTTAVTIPVFASPFAVGGNGTSSLFGTPSRQAAFFPPAPPTFITLTTPSS
CCCCCHHHCCCCEEEEEEEEECCEEECCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCC
TYSLTETYGLSAQANTALGSAVLTYGYDFSQDASTSIDRFAVPSVQSPLATRSFNGVFLQ
CEEEEHCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCHHEECCCCCCCCHHCCCCCEEEE
GSYDITPSFTVAGGVRYDTYDQNTNTGLVNNANRVTFNAGAIYSITPELALRANFAQGFR
CCCCCCCCEEEECCEEECCCCCCCCCCEEECCCEEEEECCEEEEECHHHHEEECHHHCCC
PPSLLFLFGSSPEGTFFAPSAGNVQANPNLRPERADNFDIGINYTSPEFRAGITYFNNQV
CCEEEEEECCCCCCCEECCCCCCEECCCCCCCCCCCCEEEEEEECCCHHHCCEEEECCHH
TDFLGFGPLVAFGAPPFGPPPPSQTVLNKNVQLQGLEFSSAYFFSPQAFFEANLTYVDGR
HHHHCCCHHHHCCCCCCCCCCCCHHHHCCCEEEEEEEECCEEEECCHHHEEECEEEEECC
DGSGLPLSQLEVFPLTALLRLVYDDKTFNALLQSRIYGGQGSVIGNNGVVGPGTPPAGVL
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEECCCCEECCCCCCCCEE
DLSFGYRFVPNVQLTVSVENVTNAQYIYPTSGFVAPGARLLGAVRAEF
ECCCCEEECCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]