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The map label for this gene is pstS [H]

Identifier: 37521603

GI number: 37521603

Start: 2184850

End: 2185899

Strand: Reverse

Name: pstS [H]

Synonym: gll2034

Alternate gene names: 37521603

Gene position: 2185899-2184850 (Counterclockwise)

Preceding gene: 37521604

Following gene: 37521595

Centisome position: 46.92

GC content: 61.81

Gene sequence:

>1050_bases
ATGCTGGGAGCAAACCTTTTGTTGTCCCAGTGCCAGTCTTCCCCACCGGAGGGTCCCAGTGCCACCGCCCCTAGCCCCGG
CGGCGCACCGGCCGGTGCGGTGACCCTCTCCGGCGCCGGGGCCACCTTCCCGGCACCGCTCTATCAGCGGTGGTTCTACG
ATTATGGCCAGGCCAACCCGAACGTCAAAGTCAGCTACCAGGGCGTGGGCAGCGGCGCCGGCATCAAGCAGTTTCAAAGC
AAAACCGTCGATTTTGCCGCGAGCGACGTGGCAATGAGCGACGAGGAGATTGCCGCGGTGCCGGCGGATCGAGGCGTGGT
GCTATTGCCGATGACCGCCGGTAGCGTCGTGCTCATCTACAACGTACCGGGTGCGCCGGAGAATCTTAAACTCTCCCGCG
CCGTCTACAGCGATATGTTCCTGGGCAAGATTAAAAACTGGAACGACCCGAAGATTGCCGCACTCAACCCGGGTGTCAAA
CTGCCGGATCTGGCCGCCACGGTGGTGCGCCGCGCCGACGGCAGCGGCACGACCGGCGTGTTCACTCGCCACCTGAGTGC
CGTCAGCCCCGAGTGGAAAACGAAAGTCGGCGAAGGCATCGCCGTCAAATGGCCCACGGGTGTAGGCGGCAAGGGCAACG
ACGGTGTGACGGCTCAGGTCCAGCAGAACCCCGGCTCGATCGGCTACGTGGAATACGGCTACGCCACCAACAACAAGTTG
CCCTACGCTTCGCTTGAAAACAAAGAGGGCAACTATGTCCTGCCGACGCTGGAAAATTCCGCCGCGGCCCTCGCCTCGGT
AGAGTTGCCGCCCAACCTGCGCGCTTTTATCGCCGATCCGCCCGGTAAGGATTCGTATCCACTGGTCACTTACACCTGGA
TCCTCGCACCCAAGACAATGGACACCCCCGAAAAGGCGACGGCTTTGAAGGAAGTTCTCAAGTGGTGCCTCACCGAAGGG
CAAAAGTCGAGCGAATCCCTCGGATACATTGAACTGCCCAAACCGGTTACCGAGCGCGTCCTGGCGGCTGTCGAGCAAAT
TGGCAGCTAA

Upstream 100 bases:

>100_bases
GCTTGAGCTCCCCTGCGCGTAGGATCCGAGATAATTTGTGGGTCAAACCATGGTCACAAGAGCAACTTTAATCCGGCGGA
TTTTTCCTGTTTTGGCCGCC

Downstream 100 bases:

>100_bases
ATTGCCACGACAAAGTCGAAGGTATGGACTTGGCCGTTGCGCTTAAATTCGCCCCAGAGCTTGTAAAGGCCGGGTTCAGG
AAAGGTCGTGGCGAAACTGA

Product: phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLGANLLLSQCQSSPPEGPSATAPSPGGAPAGAVTLSGAGATFPAPLYQRWFYDYGQANPNVKVSYQGVGSGAGIKQFQS
KTVDFAASDVAMSDEEIAAVPADRGVVLLPMTAGSVVLIYNVPGAPENLKLSRAVYSDMFLGKIKNWNDPKIAALNPGVK
LPDLAATVVRRADGSGTTGVFTRHLSAVSPEWKTKVGEGIAVKWPTGVGGKGNDGVTAQVQQNPGSIGYVEYGYATNNKL
PYASLENKEGNYVLPTLENSAAALASVELPPNLRAFIADPPGKDSYPLVTYTWILAPKTMDTPEKATALKEVLKWCLTEG
QKSSESLGYIELPKPVTERVLAAVEQIGS

Sequences:

>Translated_349_residues
MLGANLLLSQCQSSPPEGPSATAPSPGGAPAGAVTLSGAGATFPAPLYQRWFYDYGQANPNVKVSYQGVGSGAGIKQFQS
KTVDFAASDVAMSDEEIAAVPADRGVVLLPMTAGSVVLIYNVPGAPENLKLSRAVYSDMFLGKIKNWNDPKIAALNPGVK
LPDLAATVVRRADGSGTTGVFTRHLSAVSPEWKTKVGEGIAVKWPTGVGGKGNDGVTAQVQQNPGSIGYVEYGYATNNKL
PYASLENKEGNYVLPTLENSAAALASVELPPNLRAFIADPPGKDSYPLVTYTWILAPKTMDTPEKATALKEVLKWCLTEG
QKSSESLGYIELPKPVTERVLAAVEQIGS
>Mature_349_residues
MLGANLLLSQCQSSPPEGPSATAPSPGGAPAGAVTLSGAGATFPAPLYQRWFYDYGQANPNVKVSYQGVGSGAGIKQFQS
KTVDFAASDVAMSDEEIAAVPADRGVVLLPMTAGSVVLIYNVPGAPENLKLSRAVYSDMFLGKIKNWNDPKIAALNPGVK
LPDLAATVVRRADGSGTTGVFTRHLSAVSPEWKTKVGEGIAVKWPTGVGGKGNDGVTAQVQQNPGSIGYVEYGYATNNKL
PYASLENKEGNYVLPTLENSAAALASVELPPNLRAFIADPPGKDSYPLVTYTWILAPKTMDTPEKATALKEVLKWCLTEG
QKSSESLGYIELPKPVTERVLAAVEQIGS

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=310, Percent_Identity=46.1290322580645, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36612; Mature: 36612

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGANLLLSQCQSSPPEGPSATAPSPGGAPAGAVTLSGAGATFPAPLYQRWFYDYGQANP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCC
NVKVSYQGVGSGAGIKQFQSKTVDFAASDVAMSDEEIAAVPADRGVVLLPMTAGSVVLIY
CEEEEEECCCCCCCHHHHHHHHHCEEHHCCCCCCCCEEECCCCCCEEEEEECCCCEEEEE
NVPGAPENLKLSRAVYSDMFLGKIKNWNDPKIAALNPGVKLPDLAATVVRRADGSGTTGV
ECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCHH
FTRHLSAVSPEWKTKVGEGIAVKWPTGVGGKGNDGVTAQVQQNPGSIGYVEYGYATNNKL
HHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECEEECCCC
PYASLENKEGNYVLPTLENSAAALASVELPPNLRAFIADPPGKDSYPLVTYTWILAPKTM
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEECCCCC
DTPEKATALKEVLKWCLTEGQKSSESLGYIELPKPVTERVLAAVEQIGS
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLGANLLLSQCQSSPPEGPSATAPSPGGAPAGAVTLSGAGATFPAPLYQRWFYDYGQANP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCC
NVKVSYQGVGSGAGIKQFQSKTVDFAASDVAMSDEEIAAVPADRGVVLLPMTAGSVVLIY
CEEEEEECCCCCCCHHHHHHHHHCEEHHCCCCCCCCEEECCCCCCEEEEEECCCCEEEEE
NVPGAPENLKLSRAVYSDMFLGKIKNWNDPKIAALNPGVKLPDLAATVVRRADGSGTTGV
ECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCHH
FTRHLSAVSPEWKTKVGEGIAVKWPTGVGGKGNDGVTAQVQQNPGSIGYVEYGYATNNKL
HHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECEEECCCC
PYASLENKEGNYVLPTLENSAAALASVELPPNLRAFIADPPGKDSYPLVTYTWILAPKTM
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEECCCCC
DTPEKATALKEVLKWCLTEGQKSSESLGYIELPKPVTERVLAAVEQIGS
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6321434; 6365894; 7686882; 9278503; 9298646; 2215649; 9228942; 9671506 [H]